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The map label for this gene is aceE [H]

Identifier: 50086465

GI number: 50086465

Start: 3433625

End: 3436339

Strand: Reverse

Name: aceE [H]

Synonym: ACIAD3507

Alternate gene names: 50086465

Gene position: 3436339-3433625 (Counterclockwise)

Preceding gene: 50086466

Following gene: 50086464

Centisome position: 95.49

GC content: 44.24

Gene sequence:

>2715_bases
ATGGCGTTTTATGGTGATTCCGACGCACAAGAAACCAAAGAATGGCAAGATGCATTTGACTCGGTTCTACAGCACATGGG
AACTGAGCGCGCTGCATTCTTATTGGAAAAGCTTTACCAACGTGCGATTGCAAAGCATGTTCCCATTCAACGTCTAAATA
CGCCTTATTTAAATACGATTTCGGTTGCTGAAGAACCTGCGATGCCAGGCGATCAGGATATGGAACGCCGTATTCGTGCA
TTGATCCGCTGGAACGCTTTGGCGATGGTATTGCGTGCGAATAAGACAGGTGATGATTTAGGTGGTCACTTGGCCAGCTT
TGCATCTTCAGCAACGTTATATGATGTTGGTTTTAACCACTTCTTCCGTGCCAATAGTGATAACTTTGGCGGCGACATGA
TCTATTATCAAGGTCACTGCGCACCAGGTATTTATGCACGTTCATTCCTTGAAGGCCGTTTGACTGAAGACCAGCTCAAT
AACTTCCGTCGTGAAGTGAATGGTAACGGTTTATCAAGCTACCCACATCCATATTTAATGCCTGATTACTGGCAGTTCCC
AACAGTATCGATGGGTCTTGGCCCAATCATGTCGATTTATCAAGCGCATATTCAAAAATATTTGATGAATCGTGGCTTGA
TTAAAGAAGAAGATCGTAAAGTTTGGGCATATCTTGGCGATGGCGAGATGGATGAACCTGAAAGTACAGGTGCAATCTCA
CTTGCAGGTCGTGAAAAATTAGACAACCTGATTTGGGTGGTGAACTGTAACTTACAGCGTCTTGATGGTCCTGTACGTGG
TAATGGTAAAATCATTCAAGAACTTGAATCTTTATTCCGTGGTGCGGGTTGGCGTGTCATTAAAGTGGTTTGGGGTCGTC
ATTGGGATCCGCTATTGGCCAAAGATAATTCAGGCGCACTTAAAGCCATCATGGAAGAAGCGGTTGATGGTGATTATCAA
CGTTATCAAGTAAAAGGTGGTGCATATACGCGCGCGCACTTCTTTGGTAAATATCCTGAAGCTGAAGACTTAGTGAAAAA
TCTAAGCGATGAAGATATTGATAATCTGAATCGTGGTGGTCATGACCCGTACAAAGTATACGCAGCGTATGCAGAAGCGA
TGAAGAGCACAGGCCAGCCTACGGTAATTTTGGCAAAAACTGTCAAAGGTTATGGTTTGTCTGACGAAATCGAAGCGGTT
AACAAAACCCATCAGATCAAAAAGATGCATGTTGATTCACTTAAATATGTACGTGATCGCTTCAATCTGCCATTTACCAA
TGAGCAACTTGAAGAACTACCGTTATATCGTCCAGCAGAAAACTCGCCAGAATTAAAATACATGAAGGCACGCCGTGAAG
CATTGGGTGGTTATCTGCCTGCACGTCGTAAAGAAAGTGAAGCACTGGCGATTCCAGATATTTCAATTTTTGATAGCGTT
TTAAGCGGTAGTAACGGAAAAGAACAATCCACCACGATGGTGATGGTACGTTTGATTGCTGCTTTACTTAAAGAAAAAGC
AATTAAAGACCGTGTTGTGCCAATTGTTCCAGATGAAGCACGTACCTTTGGTCTGGAAGGTATGTTCCGTCAATTGGGTA
TTTATGCTGCGCATGGTCAGAAATATACACCAGAAGACAATGAACAGCTCATGAACTATCGTGAAGCCAAAGATGGTCAC
ATGTTGCAAGAAGGGATCAATGAAGCGGGTGCGATGAGTGCATGGGCTGCGCTTGGTACCAGTTATTCAACCAATAACTT
GCCAATGATCCCAATGTACATGTACTACTCAATGTTTGGTTTCCAGCGTATTGGCGATATTGCGTGGGCAGCAGGTGATG
CGCAGGCGCAAGGTTTCTTGCTCGGTGCTACAGCGGGTCGTACCACATTAAACGGTGAAGGCTTACAGCATCAGGACGGT
CACTCACACATTCTGGCAAATACTATTCCAAACTGTATTTCTTACGATCCATGCTTTGGTTATGAATTGGCTGTTGTGGT
GCATGATGGTTTACAGCGTATGTATGTCAATCAGGAACGTGTGTTTTATTACTTAACGGTCATGAATGAAAACTATGAAC
ATCCAGAAATGCCAAAAGGTGCTGAAGAAGGTATCAAGCGCGGGATGTATTTGTTTGAAAAAGACGACAAAGCCACTGTA
CAGCTTCTTGGTTCGGGTGTGATTCTGCGTGAAGTGATTAAAGCAGCGAAAATTTTGCGTGAAGAATATCAGATCCACTC
AAATATCTGGAGTGTCACCAGCTTCAATGAGTTGTCACGTGATGGTATGGCGTGTGATGAATACAATCGCTTGCATCCGC
TTGCAGAAGAAACCAAAGAGTCTTGGGTATCTCAGCAGTTACGCGGTACAGATGGTATCGTGGTATCAGCAACAGATCAT
ATGCGTGCGTATAGCGAACAAATTCGTGCTTACCTTCCAGACAATCGTCCTTATGTCACATTGGGTACAGATGGTTATGG
TCGTTCGGATACTCGCGGCAATCTGCGTAGTTACTTTGGTGTAGATGCAGCTCACATCGTGGTCGCAACATTGAAGAAAC
TGGCAGATGAAGGTGAAGTTGAAAACCGTTTGGTCAAAGACGCGATTTCAAGCTTTGAATTGGAAACTGATCGTCCAGTC
GCATGGGCACCTCAGGCACATCCTGAAGTTCAGCCTGTTGCAGCTTACGAAGAACACTCAGGTGAGGGGAACTAA

Upstream 100 bases:

>100_bases
AATAATGATGCCAATCTGTATTCACCTGTGGGGATAACTTGCATCCCCCTAAATATGATAAAAATAGACCCCATACAAAA
GACTAAGGAAAAGGTGAGTT

Downstream 100 bases:

>100_bases
GACATGCAAGTGAAAACACCTGATATTGGCGTAGATAAAGCAACCGTTGCTGAAATTCTGGTAAAAGTCGGCGATACTAT
TTCAGAAAATGACAGTCTGA

Product: pyruvate dehydrogenase subunit E1

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 904; Mature: 903

Protein sequence:

>904_residues
MAFYGDSDAQETKEWQDAFDSVLQHMGTERAAFLLEKLYQRAIAKHVPIQRLNTPYLNTISVAEEPAMPGDQDMERRIRA
LIRWNALAMVLRANKTGDDLGGHLASFASSATLYDVGFNHFFRANSDNFGGDMIYYQGHCAPGIYARSFLEGRLTEDQLN
NFRREVNGNGLSSYPHPYLMPDYWQFPTVSMGLGPIMSIYQAHIQKYLMNRGLIKEEDRKVWAYLGDGEMDEPESTGAIS
LAGREKLDNLIWVVNCNLQRLDGPVRGNGKIIQELESLFRGAGWRVIKVVWGRHWDPLLAKDNSGALKAIMEEAVDGDYQ
RYQVKGGAYTRAHFFGKYPEAEDLVKNLSDEDIDNLNRGGHDPYKVYAAYAEAMKSTGQPTVILAKTVKGYGLSDEIEAV
NKTHQIKKMHVDSLKYVRDRFNLPFTNEQLEELPLYRPAENSPELKYMKARREALGGYLPARRKESEALAIPDISIFDSV
LSGSNGKEQSTTMVMVRLIAALLKEKAIKDRVVPIVPDEARTFGLEGMFRQLGIYAAHGQKYTPEDNEQLMNYREAKDGH
MLQEGINEAGAMSAWAALGTSYSTNNLPMIPMYMYYSMFGFQRIGDIAWAAGDAQAQGFLLGATAGRTTLNGEGLQHQDG
HSHILANTIPNCISYDPCFGYELAVVVHDGLQRMYVNQERVFYYLTVMNENYEHPEMPKGAEEGIKRGMYLFEKDDKATV
QLLGSGVILREVIKAAKILREEYQIHSNIWSVTSFNELSRDGMACDEYNRLHPLAEETKESWVSQQLRGTDGIVVSATDH
MRAYSEQIRAYLPDNRPYVTLGTDGYGRSDTRGNLRSYFGVDAAHIVVATLKKLADEGEVENRLVKDAISSFELETDRPV
AWAPQAHPEVQPVAAYEEHSGEGN

Sequences:

>Translated_904_residues
MAFYGDSDAQETKEWQDAFDSVLQHMGTERAAFLLEKLYQRAIAKHVPIQRLNTPYLNTISVAEEPAMPGDQDMERRIRA
LIRWNALAMVLRANKTGDDLGGHLASFASSATLYDVGFNHFFRANSDNFGGDMIYYQGHCAPGIYARSFLEGRLTEDQLN
NFRREVNGNGLSSYPHPYLMPDYWQFPTVSMGLGPIMSIYQAHIQKYLMNRGLIKEEDRKVWAYLGDGEMDEPESTGAIS
LAGREKLDNLIWVVNCNLQRLDGPVRGNGKIIQELESLFRGAGWRVIKVVWGRHWDPLLAKDNSGALKAIMEEAVDGDYQ
RYQVKGGAYTRAHFFGKYPEAEDLVKNLSDEDIDNLNRGGHDPYKVYAAYAEAMKSTGQPTVILAKTVKGYGLSDEIEAV
NKTHQIKKMHVDSLKYVRDRFNLPFTNEQLEELPLYRPAENSPELKYMKARREALGGYLPARRKESEALAIPDISIFDSV
LSGSNGKEQSTTMVMVRLIAALLKEKAIKDRVVPIVPDEARTFGLEGMFRQLGIYAAHGQKYTPEDNEQLMNYREAKDGH
MLQEGINEAGAMSAWAALGTSYSTNNLPMIPMYMYYSMFGFQRIGDIAWAAGDAQAQGFLLGATAGRTTLNGEGLQHQDG
HSHILANTIPNCISYDPCFGYELAVVVHDGLQRMYVNQERVFYYLTVMNENYEHPEMPKGAEEGIKRGMYLFEKDDKATV
QLLGSGVILREVIKAAKILREEYQIHSNIWSVTSFNELSRDGMACDEYNRLHPLAEETKESWVSQQLRGTDGIVVSATDH
MRAYSEQIRAYLPDNRPYVTLGTDGYGRSDTRGNLRSYFGVDAAHIVVATLKKLADEGEVENRLVKDAISSFELETDRPV
AWAPQAHPEVQPVAAYEEHSGEGN
>Mature_903_residues
AFYGDSDAQETKEWQDAFDSVLQHMGTERAAFLLEKLYQRAIAKHVPIQRLNTPYLNTISVAEEPAMPGDQDMERRIRAL
IRWNALAMVLRANKTGDDLGGHLASFASSATLYDVGFNHFFRANSDNFGGDMIYYQGHCAPGIYARSFLEGRLTEDQLNN
FRREVNGNGLSSYPHPYLMPDYWQFPTVSMGLGPIMSIYQAHIQKYLMNRGLIKEEDRKVWAYLGDGEMDEPESTGAISL
AGREKLDNLIWVVNCNLQRLDGPVRGNGKIIQELESLFRGAGWRVIKVVWGRHWDPLLAKDNSGALKAIMEEAVDGDYQR
YQVKGGAYTRAHFFGKYPEAEDLVKNLSDEDIDNLNRGGHDPYKVYAAYAEAMKSTGQPTVILAKTVKGYGLSDEIEAVN
KTHQIKKMHVDSLKYVRDRFNLPFTNEQLEELPLYRPAENSPELKYMKARREALGGYLPARRKESEALAIPDISIFDSVL
SGSNGKEQSTTMVMVRLIAALLKEKAIKDRVVPIVPDEARTFGLEGMFRQLGIYAAHGQKYTPEDNEQLMNYREAKDGHM
LQEGINEAGAMSAWAALGTSYSTNNLPMIPMYMYYSMFGFQRIGDIAWAAGDAQAQGFLLGATAGRTTLNGEGLQHQDGH
SHILANTIPNCISYDPCFGYELAVVVHDGLQRMYVNQERVFYYLTVMNENYEHPEMPKGAEEGIKRGMYLFEKDDKATVQ
LLGSGVILREVIKAAKILREEYQIHSNIWSVTSFNELSRDGMACDEYNRLHPLAEETKESWVSQQLRGTDGIVVSATDHM
RAYSEQIRAYLPDNRPYVTLGTDGYGRSDTRGNLRSYFGVDAAHIVVATLKKLADEGEVENRLVKDAISSFELETDRPVA
WAPQAHPEVQPVAAYEEHSGEGN

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG2609

COG function: function code C; Pyruvate dehydrogenase complex, dehydrogenase (E1) component

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786304, Length=882, Percent_Identity=57.1428571428571, Blast_Score=1056, Evalue=0.0,

Paralogues:

None

Copy number: 1140 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 400 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004660
- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005474 [H]

Pfam domain/function: PF00456 Transketolase_N [H]

EC number: =1.2.4.1 [H]

Molecular weight: Translated: 101705; Mature: 101574

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: PS50003 PH_DOMAIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFYGDSDAQETKEWQDAFDSVLQHMGTERAAFLLEKLYQRAIAKHVPIQRLNTPYLNTI
CCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCEE
SVAEEPAMPGDQDMERRIRALIRWNALAMVLRANKTGDDLGGHLASFASSATLYDVGFNH
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCEEEECCCHH
FFRANSDNFGGDMIYYQGHCAPGIYARSFLEGRLTEDQLNNFRREVNGNGLSSYPHPYLM
HEECCCCCCCCCEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCC
PDYWQFPTVSMGLGPIMSIYQAHIQKYLMNRGLIKEEDRKVWAYLGDGEMDEPESTGAIS
CCCCCCCCCHHCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCEEE
LAGREKLDNLIWVVNCNLQRLDGPVRGNGKIIQELESLFRGAGWRVIKVVWGRHWDPLLA
ECCHHHHCCEEEEEECCHHHCCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCEE
KDNSGALKAIMEEAVDGDYQRYQVKGGAYTRAHFFGKYPEAEDLVKNLSDEDIDNLNRGG
CCCCHHHHHHHHHHHCCCCEEEEECCCCEEEHHHCCCCCCHHHHHHCCCCCCHHHHCCCC
HDPYKVYAAYAEAMKSTGQPTVILAKTVKGYGLSDEIEAVNKTHQIKKMHVDSLKYVRDR
CCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
FNLPFTNEQLEELPLYRPAENSPELKYMKARREALGGYLPARRKESEALAIPDISIFDSV
CCCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEECCCHHHHHHH
LSGSNGKEQSTTMVMVRLIAALLKEKAIKDRVVPIVPDEARTFGLEGMFRQLGIYAAHGQ
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCEEEECCC
KYTPEDNEQLMNYREAKDGHMLQEGINEAGAMSAWAALGTSYSTNNLPMIPMYMYYSMFG
CCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
FQRIGDIAWAAGDAQAQGFLLGATAGRTTLNGEGLQHQDGHSHILANTIPNCISYDPCFG
HHHHHHHHEECCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCEEHHHHCCCCCCCCCCCC
YELAVVVHDGLQRMYVNQERVFYYLTVMNENYEHPEMPKGAEEGIKRGMYLFEKDDKATV
CEEEEEEHHHHHHHHCCCCEEEEEEEEECCCCCCCCCCCCHHHHHHCCCEEEECCCCHHH
QLLGSGVILREVIKAAKILREEYQIHSNIWSVTSFNELSRDGMACDEYNRLHPLAEETKE
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCHHHHHCCCHHHHHHH
SWVSQQLRGTDGIVVSATDHMRAYSEQIRAYLPDNRPYVTLGTDGYGRSDTRGNLRSYFG
HHHHHHHCCCCCEEEECHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCHHHHHC
VDAAHIVVATLKKLADEGEVENRLVKDAISSFELETDRPVAWAPQAHPEVQPVAAYEEHS
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCHHHHCCC
GEGN
CCCC
>Mature Secondary Structure 
AFYGDSDAQETKEWQDAFDSVLQHMGTERAAFLLEKLYQRAIAKHVPIQRLNTPYLNTI
CCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCEE
SVAEEPAMPGDQDMERRIRALIRWNALAMVLRANKTGDDLGGHLASFASSATLYDVGFNH
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCEEEECCCHH
FFRANSDNFGGDMIYYQGHCAPGIYARSFLEGRLTEDQLNNFRREVNGNGLSSYPHPYLM
HEECCCCCCCCCEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCC
PDYWQFPTVSMGLGPIMSIYQAHIQKYLMNRGLIKEEDRKVWAYLGDGEMDEPESTGAIS
CCCCCCCCCHHCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCEEE
LAGREKLDNLIWVVNCNLQRLDGPVRGNGKIIQELESLFRGAGWRVIKVVWGRHWDPLLA
ECCHHHHCCEEEEEECCHHHCCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCEE
KDNSGALKAIMEEAVDGDYQRYQVKGGAYTRAHFFGKYPEAEDLVKNLSDEDIDNLNRGG
CCCCHHHHHHHHHHHCCCCEEEEECCCCEEEHHHCCCCCCHHHHHHCCCCCCHHHHCCCC
HDPYKVYAAYAEAMKSTGQPTVILAKTVKGYGLSDEIEAVNKTHQIKKMHVDSLKYVRDR
CCHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
FNLPFTNEQLEELPLYRPAENSPELKYMKARREALGGYLPARRKESEALAIPDISIFDSV
CCCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEECCCHHHHHHH
LSGSNGKEQSTTMVMVRLIAALLKEKAIKDRVVPIVPDEARTFGLEGMFRQLGIYAAHGQ
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCEEEECCC
KYTPEDNEQLMNYREAKDGHMLQEGINEAGAMSAWAALGTSYSTNNLPMIPMYMYYSMFG
CCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
FQRIGDIAWAAGDAQAQGFLLGATAGRTTLNGEGLQHQDGHSHILANTIPNCISYDPCFG
HHHHHHHHEECCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCEEHHHHCCCCCCCCCCCC
YELAVVVHDGLQRMYVNQERVFYYLTVMNENYEHPEMPKGAEEGIKRGMYLFEKDDKATV
CEEEEEEHHHHHHHHCCCCEEEEEEEEECCCCCCCCCCCCHHHHHHCCCEEEECCCCHHH
QLLGSGVILREVIKAAKILREEYQIHSNIWSVTSFNELSRDGMACDEYNRLHPLAEETKE
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCHHHHHCCCHHHHHHH
SWVSQQLRGTDGIVVSATDHMRAYSEQIRAYLPDNRPYVTLGTDGYGRSDTRGNLRSYFG
HHHHHHHCCCCCEEEECHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCHHHHHC
VDAAHIVVATLKKLADEGEVENRLVKDAISSFELETDRPVAWAPQAHPEVQPVAAYEEHS
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCHHHHCCC
GEGN
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9171401; 10984043 [H]