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The map label for this gene is aceF [C]

Identifier: 50086464

GI number: 50086464

Start: 3431636

End: 3433621

Strand: Reverse

Name: aceF [C]

Synonym: ACIAD3506

Alternate gene names: 50086464

Gene position: 3433621-3431636 (Counterclockwise)

Preceding gene: 50086465

Following gene: 50086463

Centisome position: 95.41

GC content: 46.32

Gene sequence:

>1986_bases
ATGCAAGTGAAAACACCTGATATTGGCGTAGATAAAGCAACCGTTGCTGAAATTCTGGTAAAAGTCGGCGATACTATTTC
AGAAAATGACAGTCTGATTCTACTCGAATCGGACAAGGCTTCTGTTGAAGTACCAAGTACAGCTTCTGGCGTGGTAAAAA
GCATTTTGGTGAGCCTTGGTGATGAAGTTTCGGAAGGAACAACACTGATTGAGCTTGAAAGCGGCGACAATACTGACAAA
ACTGAATCTGAATCGGCACCAGCCCAAACTGAAGTTAAAGCAGAAACACCTGTTGAGCAAGTTGCTCCACAGGAAACCAA
ACCAGCGACTTCAACTTCGGCTGCATCATCTGTAGTTGAAGTTCAAGTGCCAGATATTGGTGTAGAAAAAGCCACTGTCG
CGGAATTGTTAGTCAGTGTCGGCGATGAAATCGCTGAAAATGACAGTCTGGTTCTACTTGAGTCGGATAAAGCTTCGGTT
GAAGTGCCAAGCACGGTTTCTGGTACGATTGAAAGTATCGAAGTGAAAGCAGGCGATACCATTCAAGAAGGTGTTCTGCT
ACTGAAAGTCAAAACAGCAGGTGCTTCGAATGCTGCACCAGCGAAACAAGAAGCTGTTGTACCTACAGCGGCACCTGTTG
CAACCAAAGCAGAAACTCAGGCTGAGACGCCAGCAGTTCAAAGCGCACCAGCTGGGGCAGTAGATGTACTCGTGCCTGAT
TTGGGCGTAGACAAGGCTGCCGTGGCTGAGATTTTGGTCAACGTGGGTGACAAGATTACCAAAGATGAAAGTATCGTTGT
GGTGGAATCAGACAAGGCGACTGTTGAAGTTCCAAGTACGGTATCTGGTATTGTCAAAGCTATTCACGTAAAAGCGGGTC
AGGATGTCAAAGAAGGCATTCTTTTAGTGACTGTAGAAGCTGAAGGTGCCGTTGCAAGCGCGCCAAAGGCACCTGTAGCA
AAAGCTGAAGCAGCTCCTGCGCCAGCGGCACAGAAAGCTGAAGCACCTGCGGCTAAAGTTGAAACAGCACCACAAGCAGG
TGCGGACAAGCTCACCAAAGAGCAAGAAGCTGAAAATTCGAAAGTCTATGCAGGTCCAGCGGTGCGTAAATTGGCACGTG
AGTTGGGTGTTGTGTTGGCTCAGGTTAAAGCATCTGGCGAGCATGGGCGTTTGATGAAAGAGGACATCTATGCGTATGTC
AAACAACGTCTAACAGCACCTGTTGCTGCGCCAAAAGCTGCGGCTGCGGCAGTTGCATCAGGCTTGCCAACATTGCCAGA
TTTCAGTGCATTTGGTGGTGTGGAAGAGAAAACGCTGACACGTTTACAGCAAGTATCGATTCCACAACTCTCTTTAAATA
ACTTTATTCCTCAAGTGACTCAGTTTGACCTTGCGGACATTACTGAACTTGAAGCATGGCGTAATGATTTAAAGGGTAAC
TTCAAAAAAGAAGGGATTAGCCTGACCATTATGGCATTTATCATTAAAGCGGTTGCGCATCTTTTGAAAGAAGAGCGTGA
ATTTGCTGGACATTTGTCTGATGATGGTAAGTCTGTATTGTTGCGTAAAGAGATTCACATGGGTATCGCGGTCGCGACAC
CAGATGGCTTGACTGTGCCTGTGCTTCGCAATCCTGATCAGAAAACGATTAAGCAAATTGCGGTTGAACTGGGTGTAATT
GGTCAAAAAGCACGTGATAAGAAGCTTTCACCAAAAGATTTACAAGGTGCAAACTTCACTATTTCAAGCCTGGGCGCGAT
TGGTGGTACGGCATTTACACCTTTGGTGAATTGGCCGCAAGTGGCAATCTTGGGTATTTCACCTGCAACTATGCAGCCTG
TATGGAATGGTAAAGACTTTGACCCGCGCTTAATGTTGCCACTGTCATTGTCTTATGATCACCGTGTAATCAATGGTGCT
GACGCTGCGCGTTTCACCAATAAGTTGACCAAGTTACTTCAGGACATCCGTAGCTTGCTTATTTAA

Upstream 100 bases:

>100_bases
ATTGGAAACTGATCGTCCAGTCGCATGGGCACCTCAGGCACATCCTGAAGTTCAGCCTGTTGCAGCTTACGAAGAACACT
CAGGTGAGGGGAACTAAGAC

Downstream 100 bases:

>100_bases
TGCATAATATTTCTTAATTCTGTTATTTCTCTAAAAACCTCGCTCTGGCGGGGTTTTTTAATGCAGTTTCATTTATCGGA
AGCGCATTAAGAACAGAAAA

Product: dihydrolipoamide S-acetyltransferase, E2 component of the pyruvate dehydrogenase complex

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 661; Mature: 661

Protein sequence:

>661_residues
MQVKTPDIGVDKATVAEILVKVGDTISENDSLILLESDKASVEVPSTASGVVKSILVSLGDEVSEGTTLIELESGDNTDK
TESESAPAQTEVKAETPVEQVAPQETKPATSTSAASSVVEVQVPDIGVEKATVAELLVSVGDEIAENDSLVLLESDKASV
EVPSTVSGTIESIEVKAGDTIQEGVLLLKVKTAGASNAAPAKQEAVVPTAAPVATKAETQAETPAVQSAPAGAVDVLVPD
LGVDKAAVAEILVNVGDKITKDESIVVVESDKATVEVPSTVSGIVKAIHVKAGQDVKEGILLVTVEAEGAVASAPKAPVA
KAEAAPAPAAQKAEAPAAKVETAPQAGADKLTKEQEAENSKVYAGPAVRKLARELGVVLAQVKASGEHGRLMKEDIYAYV
KQRLTAPVAAPKAAAAAVASGLPTLPDFSAFGGVEEKTLTRLQQVSIPQLSLNNFIPQVTQFDLADITELEAWRNDLKGN
FKKEGISLTIMAFIIKAVAHLLKEEREFAGHLSDDGKSVLLRKEIHMGIAVATPDGLTVPVLRNPDQKTIKQIAVELGVI
GQKARDKKLSPKDLQGANFTISSLGAIGGTAFTPLVNWPQVAILGISPATMQPVWNGKDFDPRLMLPLSLSYDHRVINGA
DAARFTNKLTKLLQDIRSLLI

Sequences:

>Translated_661_residues
MQVKTPDIGVDKATVAEILVKVGDTISENDSLILLESDKASVEVPSTASGVVKSILVSLGDEVSEGTTLIELESGDNTDK
TESESAPAQTEVKAETPVEQVAPQETKPATSTSAASSVVEVQVPDIGVEKATVAELLVSVGDEIAENDSLVLLESDKASV
EVPSTVSGTIESIEVKAGDTIQEGVLLLKVKTAGASNAAPAKQEAVVPTAAPVATKAETQAETPAVQSAPAGAVDVLVPD
LGVDKAAVAEILVNVGDKITKDESIVVVESDKATVEVPSTVSGIVKAIHVKAGQDVKEGILLVTVEAEGAVASAPKAPVA
KAEAAPAPAAQKAEAPAAKVETAPQAGADKLTKEQEAENSKVYAGPAVRKLARELGVVLAQVKASGEHGRLMKEDIYAYV
KQRLTAPVAAPKAAAAAVASGLPTLPDFSAFGGVEEKTLTRLQQVSIPQLSLNNFIPQVTQFDLADITELEAWRNDLKGN
FKKEGISLTIMAFIIKAVAHLLKEEREFAGHLSDDGKSVLLRKEIHMGIAVATPDGLTVPVLRNPDQKTIKQIAVELGVI
GQKARDKKLSPKDLQGANFTISSLGAIGGTAFTPLVNWPQVAILGISPATMQPVWNGKDFDPRLMLPLSLSYDHRVINGA
DAARFTNKLTKLLQDIRSLLI
>Mature_661_residues
MQVKTPDIGVDKATVAEILVKVGDTISENDSLILLESDKASVEVPSTASGVVKSILVSLGDEVSEGTTLIELESGDNTDK
TESESAPAQTEVKAETPVEQVAPQETKPATSTSAASSVVEVQVPDIGVEKATVAELLVSVGDEIAENDSLVLLESDKASV
EVPSTVSGTIESIEVKAGDTIQEGVLLLKVKTAGASNAAPAKQEAVVPTAAPVATKAETQAETPAVQSAPAGAVDVLVPD
LGVDKAAVAEILVNVGDKITKDESIVVVESDKATVEVPSTVSGIVKAIHVKAGQDVKEGILLVTVEAEGAVASAPKAPVA
KAEAAPAPAAQKAEAPAAKVETAPQAGADKLTKEQEAENSKVYAGPAVRKLARELGVVLAQVKASGEHGRLMKEDIYAYV
KQRLTAPVAAPKAAAAAVASGLPTLPDFSAFGGVEEKTLTRLQQVSIPQLSLNNFIPQVTQFDLADITELEAWRNDLKGN
FKKEGISLTIMAFIIKAVAHLLKEEREFAGHLSDDGKSVLLRKEIHMGIAVATPDGLTVPVLRNPDQKTIKQIAVELGVI
GQKARDKKLSPKDLQGANFTISSLGAIGGTAFTPLVNWPQVAILGISPATMQPVWNGKDFDPRLMLPLSLSYDHRVINGA
DAARFTNKLTKLLQDIRSLLI

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 lipoyl-binding domains [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=432, Percent_Identity=30.0925925925926, Blast_Score=152, Evalue=1e-36,
Organism=Homo sapiens, GI19923748, Length=229, Percent_Identity=34.061135371179, Blast_Score=129, Evalue=8e-30,
Organism=Homo sapiens, GI31711992, Length=442, Percent_Identity=29.185520361991, Blast_Score=122, Evalue=2e-27,
Organism=Homo sapiens, GI203098816, Length=468, Percent_Identity=24.7863247863248, Blast_Score=104, Evalue=3e-22,
Organism=Homo sapiens, GI203098753, Length=459, Percent_Identity=24.8366013071895, Blast_Score=102, Evalue=2e-21,
Organism=Homo sapiens, GI260898739, Length=139, Percent_Identity=33.8129496402878, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI1786305, Length=667, Percent_Identity=41.5292353823088, Blast_Score=434, Evalue=1e-123,
Organism=Escherichia coli, GI1786946, Length=442, Percent_Identity=32.1266968325792, Blast_Score=159, Evalue=7e-40,
Organism=Caenorhabditis elegans, GI17537937, Length=432, Percent_Identity=29.8611111111111, Blast_Score=161, Evalue=9e-40,
Organism=Caenorhabditis elegans, GI25146366, Length=211, Percent_Identity=36.4928909952607, Blast_Score=128, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17560088, Length=432, Percent_Identity=28.2407407407407, Blast_Score=113, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17538894, Length=321, Percent_Identity=26.4797507788162, Blast_Score=91, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6320352, Length=427, Percent_Identity=29.03981264637, Blast_Score=132, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6324258, Length=182, Percent_Identity=29.6703296703297, Blast_Score=85, Evalue=4e-17,
Organism=Drosophila melanogaster, GI18859875, Length=426, Percent_Identity=29.1079812206573, Blast_Score=142, Evalue=7e-34,
Organism=Drosophila melanogaster, GI24645909, Length=197, Percent_Identity=32.994923857868, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24582497, Length=202, Percent_Identity=31.6831683168317, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI20129315, Length=202, Percent_Identity=31.6831683168317, Blast_Score=100, Evalue=4e-21,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006256
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 68975; Mature: 68975

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQVKTPDIGVDKATVAEILVKVGDTISENDSLILLESDKASVEVPSTASGVVKSILVSLG
CCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCCEECCCCHHHHHHHHHHHHC
DEVSEGTTLIELESGDNTDKTESESAPAQTEVKAETPVEQVAPQETKPATSTSAASSVVE
CCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCHHHHCCEEE
VQVPDIGVEKATVAELLVSVGDEIAENDSLVLLESDKASVEVPSTVSGTIESIEVKAGDT
EECCCCCCCHHHHHHHHHHHCHHHCCCCCEEEEECCCCCEECCCCCCCCEEEEEECCCCC
IQEGVLLLKVKTAGASNAAPAKQEAVVPTAAPVATKAETQAETPAVQSAPAGAVDVLVPD
HHCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCEEEEEECC
LGVDKAAVAEILVNVGDKITKDESIVVVESDKATVEVPSTVSGIVKAIHVKAGQDVKEGI
CCCCHHHHHHHHHHCCCCCCCCCEEEEEECCCCEEECCHHHHHHHHHHHHCCCCCHHCCE
LLVTVEAEGAVASAPKAPVAKAEAAPAPAAQKAEAPAAKVETAPQAGADKLTKEQEAENS
EEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHCCCCHHHHCCCCCCCHHHHHHHHHCCCC
KVYAGPAVRKLARELGVVLAQVKASGEHGRLMKEDIYAYVKQRLTAPVAAPKAAAAAVAS
EEEECHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHC
GLPTLPDFSAFGGVEEKTLTRLQQVSIPQLSLNNFIPQVTQFDLADITELEAWRNDLKGN
CCCCCCCHHHHCCCCHHHHHHHHHCCCCCCHHHHHCCHHHHCCHHHHHHHHHHHHHHCCC
FKKEGISLTIMAFIIKAVAHLLKEEREFAGHLSDDGKSVLLRKEIHMGIAVATPDGLTVP
CHHCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCEEEECCCCCEEE
VLRNPDQKTIKQIAVELGVIGQKARDKKLSPKDLQGANFTISSLGAIGGTAFTPLVNWPQ
EECCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEHHHHHCCCCCHHCCCCCCCC
VAILGISPATMQPVWNGKDFDPRLMLPLSLSYDHRVINGADAARFTNKLTKLLQDIRSLL
EEEEECCCHHCCCCCCCCCCCCEEEEEEECCCCCEEECCCHHHHHHHHHHHHHHHHHHHH
I
C
>Mature Secondary Structure
MQVKTPDIGVDKATVAEILVKVGDTISENDSLILLESDKASVEVPSTASGVVKSILVSLG
CCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCCEECCCCHHHHHHHHHHHHC
DEVSEGTTLIELESGDNTDKTESESAPAQTEVKAETPVEQVAPQETKPATSTSAASSVVE
CCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCHHHHCCEEE
VQVPDIGVEKATVAELLVSVGDEIAENDSLVLLESDKASVEVPSTVSGTIESIEVKAGDT
EECCCCCCCHHHHHHHHHHHCHHHCCCCCEEEEECCCCCEECCCCCCCCEEEEEECCCCC
IQEGVLLLKVKTAGASNAAPAKQEAVVPTAAPVATKAETQAETPAVQSAPAGAVDVLVPD
HHCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCEEEEEECC
LGVDKAAVAEILVNVGDKITKDESIVVVESDKATVEVPSTVSGIVKAIHVKAGQDVKEGI
CCCCHHHHHHHHHHCCCCCCCCCEEEEEECCCCEEECCHHHHHHHHHHHHCCCCCHHCCE
LLVTVEAEGAVASAPKAPVAKAEAAPAPAAQKAEAPAAKVETAPQAGADKLTKEQEAENS
EEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHCCCCHHHHCCCCCCCHHHHHHHHHCCCC
KVYAGPAVRKLARELGVVLAQVKASGEHGRLMKEDIYAYVKQRLTAPVAAPKAAAAAVAS
EEEECHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHC
GLPTLPDFSAFGGVEEKTLTRLQQVSIPQLSLNNFIPQVTQFDLADITELEAWRNDLKGN
CCCCCCCHHHHCCCCHHHHHHHHHCCCCCCHHHHHCCHHHHCCHHHHHHHHHHHHHHCCC
FKKEGISLTIMAFIIKAVAHLLKEEREFAGHLSDDGKSVLLRKEIHMGIAVATPDGLTVP
CHHCCCEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCEEEECCCCCEEE
VLRNPDQKTIKQIAVELGVIGQKARDKKLSPKDLQGANFTISSLGAIGGTAFTPLVNWPQ
EECCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEHHHHHCCCCCHHCCCCCCCC
VAILGISPATMQPVWNGKDFDPRLMLPLSLSYDHRVINGADAARFTNKLTKLLQDIRSLL
EEEEECCCHHCCCCCCCCCCCCEEEEEEECCCCCEEECCCHHHHHHHHHHHHHHHHHHHH
I
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3292237; 3691494; 3191993; 1549782; 8068086; 9119000 [H]