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The map label for this gene is ynfL [H]

Identifier: 50086451

GI number: 50086451

Start: 3417471

End: 3418457

Strand: Reverse

Name: ynfL [H]

Synonym: ACIAD3490

Alternate gene names: 50086451

Gene position: 3418457-3417471 (Counterclockwise)

Preceding gene: 50086452

Following gene: 50086450

Centisome position: 94.99

GC content: 41.03

Gene sequence:

>987_bases
GTGCTAAATCGTATTTCATTGCGTCAAATACAATATTTTGTTGCCACTGCTGAAAGTGGCAGCATTATCATTGCCTCTGA
AAAAATTCATGTTTCTTCTCCTTCCATTTCAGCCGCGATTGCGCATATAGAATCAGAACTTCAGGCACAACTATTTATTC
GCCAGCATGCCAAAGGGTTAATTTTGACCGATATTGGTGAAAAGGTGATGCAGGAGTGCAAGGTGCTTCTGAGTCAGGCA
GATAATCTTTACGCACTGGTTGCAGACTTTTCTGGTTCAGTACGAGGGCCGTTGAAACTTGGTTGTTTTACAGCATTTGC
GCCGATGATTCATGCTGAGGTCATTCAGGGATTTTGTAACTTATATAAAAATGTAACGCTGAATGTTTCTGAAGACGATC
AAATGGTGCTGATGAATCGTTTAATCCGTAATGAACTGGATGTAGCGCTTACCTATGATTTAAATCTGGATGATGATTTG
ATTAACTTTGAATCACTGGCGATTTTACCGCCGCATGTTCTGGTCAGCGAATCCCACCCATTAGCACAGAACAGTGCTGT
GACATTAGAGGAATTGACAGAACTGCCGATGGTGCTCTTAGACATGCCATATAGTAATGATTATTTTATTTCATTATTTA
GAAAGTATAAATTAAGTCCCAAAGTCACTCATTCTTCCCGGCATGTCGATGTTGTTCGCTCTATGGTCGCGAATAATTTG
GGCTACACCATTTTAAATGTAAGGCCAAAAAACAATTATTCATTAGATGGTAAGCGTTTGGTGCGGTTACGGATCGCGGG
TTCACATCGTCCTATGCATATTGGCCTGGCAACTGCAAAAAATGCCACATTAAACACGGTGGCACAGGCATTTATTAGCC
GCTGTCGTGCTTTTATTTCCAATCATTATATTTCAGGCATGACCGATGCCCATTTCTTTGACCCACATATTAAATCTCAT
GCACTCGATGTTCAGGAACCTGAGTAG

Upstream 100 bases:

>100_bases
TTATGTTATTCGGGCACTGAATCCAAGCGACTCTCCGAGCTATGCTGTTCCAGGCGATGAATGATACATTGATGGTGTTC
ACTGATAAGGTTTAAAACAC

Downstream 100 bases:

>100_bases
ATGAATAAGCAGATTAGAAGTTTAGCGTTATTGGAACGGTTGATTGCATTTGATACGACATCTTATAAAAGTAATATCGA
ACTGATTGATGATGTAAAGC

Product: transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MLNRISLRQIQYFVATAESGSIIIASEKIHVSSPSISAAIAHIESELQAQLFIRQHAKGLILTDIGEKVMQECKVLLSQA
DNLYALVADFSGSVRGPLKLGCFTAFAPMIHAEVIQGFCNLYKNVTLNVSEDDQMVLMNRLIRNELDVALTYDLNLDDDL
INFESLAILPPHVLVSESHPLAQNSAVTLEELTELPMVLLDMPYSNDYFISLFRKYKLSPKVTHSSRHVDVVRSMVANNL
GYTILNVRPKNNYSLDGKRLVRLRIAGSHRPMHIGLATAKNATLNTVAQAFISRCRAFISNHYISGMTDAHFFDPHIKSH
ALDVQEPE

Sequences:

>Translated_328_residues
MLNRISLRQIQYFVATAESGSIIIASEKIHVSSPSISAAIAHIESELQAQLFIRQHAKGLILTDIGEKVMQECKVLLSQA
DNLYALVADFSGSVRGPLKLGCFTAFAPMIHAEVIQGFCNLYKNVTLNVSEDDQMVLMNRLIRNELDVALTYDLNLDDDL
INFESLAILPPHVLVSESHPLAQNSAVTLEELTELPMVLLDMPYSNDYFISLFRKYKLSPKVTHSSRHVDVVRSMVANNL
GYTILNVRPKNNYSLDGKRLVRLRIAGSHRPMHIGLATAKNATLNTVAQAFISRCRAFISNHYISGMTDAHFFDPHIKSH
ALDVQEPE
>Mature_328_residues
MLNRISLRQIQYFVATAESGSIIIASEKIHVSSPSISAAIAHIESELQAQLFIRQHAKGLILTDIGEKVMQECKVLLSQA
DNLYALVADFSGSVRGPLKLGCFTAFAPMIHAEVIQGFCNLYKNVTLNVSEDDQMVLMNRLIRNELDVALTYDLNLDDDL
INFESLAILPPHVLVSESHPLAQNSAVTLEELTELPMVLLDMPYSNDYFISLFRKYKLSPKVTHSSRHVDVVRSMVANNL
GYTILNVRPKNNYSLDGKRLVRLRIAGSHRPMHIGLATAKNATLNTVAQAFISRCRAFISNHYISGMTDAHFFDPHIKSH
ALDVQEPE

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787879, Length=251, Percent_Identity=30.6772908366534, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1787530, Length=249, Percent_Identity=23.6947791164659, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI157672245, Length=243, Percent_Identity=28.3950617283951, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI2367136, Length=245, Percent_Identity=27.7551020408163, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI145693105, Length=309, Percent_Identity=24.9190938511327, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1787601, Length=181, Percent_Identity=28.7292817679558, Blast_Score=75, Evalue=9e-15,
Organism=Escherichia coli, GI1789204, Length=280, Percent_Identity=22.8571428571429, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788297, Length=154, Percent_Identity=27.9220779220779, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1788296, Length=219, Percent_Identity=25.1141552511416, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1788887, Length=253, Percent_Identity=25.296442687747, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1790399, Length=309, Percent_Identity=22.6537216828479, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 36613; Mature: 36613

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNRISLRQIQYFVATAESGSIIIASEKIHVSSPSISAAIAHIESELQAQLFIRQHAKGL
CCCCHHHHHEEEEEEECCCCCEEEEECEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCE
ILTDIGEKVMQECKVLLSQADNLYALVADFSGSVRGPLKLGCFTAFAPMIHAEVIQGFCN
EEEHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LYKNVTLNVSEDDQMVLMNRLIRNELDVALTYDLNLDDDLINFESLAILPPHVLVSESHP
HHCCEEEEECCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCEEECCCEEEEECCCC
LAQNSAVTLEELTELPMVLLDMPYSNDYFISLFRKYKLSPKVTHSSRHVDVVRSMVANNL
CCCCCCEEHHHHHHCCEEEEECCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCC
GYTILNVRPKNNYSLDGKRLVRLRIAGSHRPMHIGLATAKNATLNTVAQAFISRCRAFIS
CEEEEEEECCCCCCCCCEEEEEEEEECCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHH
NHYISGMTDAHFFDPHIKSHALDVQEPE
CCCCCCCCCCCCCCCCHHHCCCCCCCCC
>Mature Secondary Structure
MLNRISLRQIQYFVATAESGSIIIASEKIHVSSPSISAAIAHIESELQAQLFIRQHAKGL
CCCCHHHHHEEEEEEECCCCCEEEEECEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCE
ILTDIGEKVMQECKVLLSQADNLYALVADFSGSVRGPLKLGCFTAFAPMIHAEVIQGFCN
EEEHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LYKNVTLNVSEDDQMVLMNRLIRNELDVALTYDLNLDDDLINFESLAILPPHVLVSESHP
HHCCEEEEECCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCEEECCCEEEEECCCC
LAQNSAVTLEELTELPMVLLDMPYSNDYFISLFRKYKLSPKVTHSSRHVDVVRSMVANNL
CCCCCCEEHHHHHHCCEEEEECCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCC
GYTILNVRPKNNYSLDGKRLVRLRIAGSHRPMHIGLATAKNATLNTVAQAFISRCRAFIS
CEEEEEEECCCCCCCCCEEEEEEEEECCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHH
NHYISGMTDAHFFDPHIKSHALDVQEPE
CCCCCCCCCCCCCCCCHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]