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The map label for this gene is betT [H]

Identifier: 50086423

GI number: 50086423

Start: 3384555

End: 3386537

Strand: Reverse

Name: betT [H]

Synonym: ACIAD3460

Alternate gene names: 50086423

Gene position: 3386537-3384555 (Counterclockwise)

Preceding gene: 50086424

Following gene: 50086421

Centisome position: 94.11

GC content: 41.86

Gene sequence:

>1983_bases
ATGCCTTCCAAAACCTCATCTAGGTTTGCAAACATTAATCCAAATGTATTTGTAAGTACGATTATGATCATTGCAATTTT
TCTTGCAATTGTAATTTTAGCTCCAGATGCATTTGAATTGTTGACTCAACAATTAAAAAACTGGATTACAGAATCATTTA
GTTGGTTCTATGTATTATCGGTTGCCTTCTTTCTAATTGTACTCGGTTATATCGCCTGCTCATCGAGCGGAAAAATCAAG
TTAGGTCCAGATCATAGTCAGCCTGATTATAGTAATAGCTCTTGGTTTGCCATGCTATTTACTGCTGGTATGGGCATCGG
ACTCATGTTCTTCGGGATTGCTGAACCGATTATGCATTATGTGAGCCCACCTTCTGGTGAGCCAGAAACAATTTTAGCTG
CCCAGCAATCTATGCGCGTAACCTTCTTCCATTGGGGATTACACGCCTGGGGGATTTACGCGATTGTGGCATTATCTTTG
TCTTATTTTGCTTATCGTCATGATTTACCCTTAAAGATTCGCTCTTCTCTTTATCCTCTTATTGGTAAGAAAATTTATGG
ACCGATGGGAGATGCAGTTGATACCTTTGCGACCATTGGGACAATTTTTGGTGTTGCTACAACGCTCGGCTTTGGGGTTA
CCCAAATTAGCTCTGGCTTAAATTACTTATTCGGATTTGAACCCACTTCTTTTTCTAAGGTTGTTCTGATTATTATCGTG
AGTGCCATGGCTGCCTTGTCGGTCGGGTTGGGTCTGGACAAAGGGGTAAAACGCCTTGCAGAGTTAAATCTGGTCTTGGC
TGTCACGCTTTTAGCGTTTGTTTTCTTCACGAGTGCAACGGTTTATTTGCTCCAAACGACAATCCAGAATACGGGTCAAT
ATATTTCCAATCTTTTTGAAATGACCTTTAATCTCTATGCCTATCAACCCAATGGCTGGATTGGTGGCTGGACAATTATG
TACTGGGCATGGTGGATTTCTTGGTCGCCATTTGTGGGGATGTTTATTGCACGTGTATCACGCGGACGTACCATTCGAGA
GTTTATTATTGGTGTCATGCTGATTCCGACAGGTTTTACCCTGATCTGGATGGGATTCATGGGTAATGCTGGCTTGTATA
GCATTTTGCATGACGGTAATCTCAGCCTGCTTAATGCAGTACAACGTGACTCATCTGTTGCTTTGTTTGAATTTTTACAT
AGTTTACCTTTTTCTGGTGTGATGAGCCTATTGGCGACGGTGCTGGTGGTTTTATTCTTTGTGACCTCTGCAGATTCGGG
CGCATTGGTGGTGGATTATCTGACAGCCAAGTCTGAAGATTCTCCAGTATGGCAGCGTTTATTTTGGATTGTAGTGATGG
CAGGTCTGGCGATTATCTTGCTATTGGCCGGTGGTTTGACTGCGTTACAATCTGCTACGATCATGAGTGCTTTACCATTT
ACCTTTATCATGTTGCTCATTTGTTGGGGACTGATTAAAGCGCTCAGGATCGACTCAACCAAAATGCAAGCGATTCAGGA
AGCGAGAACTACACCACGTGCGATACAAAATCCACGTAGTTGGCAGCAACGCCTTGGATTGATCATGCATTATCCGCACT
CTAAAGTGGAGGTAGATGCTTATATCAAAAAGCATGTTCAGCGTGCTTTTGAAAGTCTGGAACGTGAGTTTAAGCGTCGT
CATCTTACTGTGGCGATTAGTGAAACAGATGATGGCCTACAGCTTAAAGTGGATCATCATGATGAAATCAATTTTATTTA
TCATGTTGTTTCACGTGAAACAATGCCACCAAGTTTTATGCTAGAACAGGAACATAACGCGGATGTTGAAAAATACTTTC
AGGCCGAAGTGTTTTTGAGAGAAGGTGGACAGAATTACGACGTAATGGACTGGACTGAAGAAGATTTAATTCAGGATATT
ATCGATCAGTACGAACGTCATCTCTACTTCTTGAGTGTAATGCGTGCTCAAACTGGAAATTAA

Upstream 100 bases:

>100_bases
AGCTCGCGCAGTTTGTGCTAATTTTACATTTTTCACGATAGTGAAGCTTTCTTTGACATTTTTTTGTCATCCGTTAAATG
AAGAATAACATGGGGGATCT

Downstream 100 bases:

>100_bases
ATATTCAGACGTAAAAAAGGACTCATAAGAGTCCTTTTTTATAGGTCGATCATTTCGAATTAGAAACGAGCTTTAGCATT
CAAACCGATTGTTTGAGTGT

Product: high affinity choline transport protein (Bet-like)

Products: Proton [Cytoplasm]; choline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 660; Mature: 659

Protein sequence:

>660_residues
MPSKTSSRFANINPNVFVSTIMIIAIFLAIVILAPDAFELLTQQLKNWITESFSWFYVLSVAFFLIVLGYIACSSSGKIK
LGPDHSQPDYSNSSWFAMLFTAGMGIGLMFFGIAEPIMHYVSPPSGEPETILAAQQSMRVTFFHWGLHAWGIYAIVALSL
SYFAYRHDLPLKIRSSLYPLIGKKIYGPMGDAVDTFATIGTIFGVATTLGFGVTQISSGLNYLFGFEPTSFSKVVLIIIV
SAMAALSVGLGLDKGVKRLAELNLVLAVTLLAFVFFTSATVYLLQTTIQNTGQYISNLFEMTFNLYAYQPNGWIGGWTIM
YWAWWISWSPFVGMFIARVSRGRTIREFIIGVMLIPTGFTLIWMGFMGNAGLYSILHDGNLSLLNAVQRDSSVALFEFLH
SLPFSGVMSLLATVLVVLFFVTSADSGALVVDYLTAKSEDSPVWQRLFWIVVMAGLAIILLLAGGLTALQSATIMSALPF
TFIMLLICWGLIKALRIDSTKMQAIQEARTTPRAIQNPRSWQQRLGLIMHYPHSKVEVDAYIKKHVQRAFESLEREFKRR
HLTVAISETDDGLQLKVDHHDEINFIYHVVSRETMPPSFMLEQEHNADVEKYFQAEVFLREGGQNYDVMDWTEEDLIQDI
IDQYERHLYFLSVMRAQTGN

Sequences:

>Translated_660_residues
MPSKTSSRFANINPNVFVSTIMIIAIFLAIVILAPDAFELLTQQLKNWITESFSWFYVLSVAFFLIVLGYIACSSSGKIK
LGPDHSQPDYSNSSWFAMLFTAGMGIGLMFFGIAEPIMHYVSPPSGEPETILAAQQSMRVTFFHWGLHAWGIYAIVALSL
SYFAYRHDLPLKIRSSLYPLIGKKIYGPMGDAVDTFATIGTIFGVATTLGFGVTQISSGLNYLFGFEPTSFSKVVLIIIV
SAMAALSVGLGLDKGVKRLAELNLVLAVTLLAFVFFTSATVYLLQTTIQNTGQYISNLFEMTFNLYAYQPNGWIGGWTIM
YWAWWISWSPFVGMFIARVSRGRTIREFIIGVMLIPTGFTLIWMGFMGNAGLYSILHDGNLSLLNAVQRDSSVALFEFLH
SLPFSGVMSLLATVLVVLFFVTSADSGALVVDYLTAKSEDSPVWQRLFWIVVMAGLAIILLLAGGLTALQSATIMSALPF
TFIMLLICWGLIKALRIDSTKMQAIQEARTTPRAIQNPRSWQQRLGLIMHYPHSKVEVDAYIKKHVQRAFESLEREFKRR
HLTVAISETDDGLQLKVDHHDEINFIYHVVSRETMPPSFMLEQEHNADVEKYFQAEVFLREGGQNYDVMDWTEEDLIQDI
IDQYERHLYFLSVMRAQTGN
>Mature_659_residues
PSKTSSRFANINPNVFVSTIMIIAIFLAIVILAPDAFELLTQQLKNWITESFSWFYVLSVAFFLIVLGYIACSSSGKIKL
GPDHSQPDYSNSSWFAMLFTAGMGIGLMFFGIAEPIMHYVSPPSGEPETILAAQQSMRVTFFHWGLHAWGIYAIVALSLS
YFAYRHDLPLKIRSSLYPLIGKKIYGPMGDAVDTFATIGTIFGVATTLGFGVTQISSGLNYLFGFEPTSFSKVVLIIIVS
AMAALSVGLGLDKGVKRLAELNLVLAVTLLAFVFFTSATVYLLQTTIQNTGQYISNLFEMTFNLYAYQPNGWIGGWTIMY
WAWWISWSPFVGMFIARVSRGRTIREFIIGVMLIPTGFTLIWMGFMGNAGLYSILHDGNLSLLNAVQRDSSVALFEFLHS
LPFSGVMSLLATVLVVLFFVTSADSGALVVDYLTAKSEDSPVWQRLFWIVVMAGLAIILLLAGGLTALQSATIMSALPFT
FIMLLICWGLIKALRIDSTKMQAIQEARTTPRAIQNPRSWQQRLGLIMHYPHSKVEVDAYIKKHVQRAFESLEREFKRRH
LTVAISETDDGLQLKVDHHDEINFIYHVVSRETMPPSFMLEQEHNADVEKYFQAEVFLREGGQNYDVMDWTEEDLIQDII
DQYERHLYFLSVMRAQTGN

Specific function: High-affinity uptake of choline driven by a proton- motive force [H]

COG id: COG1292

COG function: function code M; Choline-glycine betaine transporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BCCT transporter (TC 2.A.15) family [H]

Homologues:

Organism=Escherichia coli, GI1786506, Length=667, Percent_Identity=39.5802098950525, Blast_Score=516, Evalue=1e-147,
Organism=Escherichia coli, GI1786224, Length=505, Percent_Identity=27.7227722772277, Blast_Score=189, Evalue=5e-49,
Organism=Escherichia coli, GI1788102, Length=454, Percent_Identity=28.8546255506608, Blast_Score=173, Evalue=3e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000060
- InterPro:   IPR018093 [H]

Pfam domain/function: PF02028 BCCT [H]

EC number: NA

Molecular weight: Translated: 74118; Mature: 73987

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS01303 BCCT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSKTSSRFANINPNVFVSTIMIIAIFLAIVILAPDAFELLTQQLKNWITESFSWFYVLS
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
VAFFLIVLGYIACSSSGKIKLGPDHSQPDYSNSSWFAMLFTAGMGIGLMFFGIAEPIMHY
HHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VSPPSGEPETILAAQQSMRVTFFHWGLHAWGIYAIVALSLSYFAYRHDLPLKIRSSLYPL
CCCCCCCCCHHHHHHHCCEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
IGKKIYGPMGDAVDTFATIGTIFGVATTLGFGVTQISSGLNYLFGFEPTSFSKVVLIIIV
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHH
SAMAALSVGLGLDKGVKRLAELNLVLAVTLLAFVFFTSATVYLLQTTIQNTGQYISNLFE
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MTFNLYAYQPNGWIGGWTIMYWAWWISWSPFVGMFIARVSRGRTIREFIIGVMLIPTGFT
HHHHEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHH
LIWMGFMGNAGLYSILHDGNLSLLNAVQRDSSVALFEFLHSLPFSGVMSLLATVLVVLFF
HHHHHHCCCCCCEEHEECCCHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHH
VTSADSGALVVDYLTAKSEDSPVWQRLFWIVVMAGLAIILLLAGGLTALQSATIMSALPF
HHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
TFIMLLICWGLIKALRIDSTKMQAIQEARTTPRAIQNPRSWQQRLGLIMHYPHSKVEVDA
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHCCHHHHHHHCCHHEECCCCCHHHHH
YIKKHVQRAFESLEREFKRRHLTVAISETDDGLQLKVDHHDEINFIYHVVSRETMPPSFM
HHHHHHHHHHHHHHHHHHHCEEEEEEEECCCCEEEEECCCCCHHHHHHHHHCCCCCCCCE
LEQEHNADVEKYFQAEVFLREGGQNYDVMDWTEEDLIQDIIDQYERHLYFLSVMRAQTGN
ECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PSKTSSRFANINPNVFVSTIMIIAIFLAIVILAPDAFELLTQQLKNWITESFSWFYVLS
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
VAFFLIVLGYIACSSSGKIKLGPDHSQPDYSNSSWFAMLFTAGMGIGLMFFGIAEPIMHY
HHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VSPPSGEPETILAAQQSMRVTFFHWGLHAWGIYAIVALSLSYFAYRHDLPLKIRSSLYPL
CCCCCCCCCHHHHHHHCCEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
IGKKIYGPMGDAVDTFATIGTIFGVATTLGFGVTQISSGLNYLFGFEPTSFSKVVLIIIV
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHH
SAMAALSVGLGLDKGVKRLAELNLVLAVTLLAFVFFTSATVYLLQTTIQNTGQYISNLFE
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MTFNLYAYQPNGWIGGWTIMYWAWWISWSPFVGMFIARVSRGRTIREFIIGVMLIPTGFT
HHHHEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHH
LIWMGFMGNAGLYSILHDGNLSLLNAVQRDSSVALFEFLHSLPFSGVMSLLATVLVVLFF
HHHHHHCCCCCCEEHEECCCHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHH
VTSADSGALVVDYLTAKSEDSPVWQRLFWIVVMAGLAIILLLAGGLTALQSATIMSALPF
HHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
TFIMLLICWGLIKALRIDSTKMQAIQEARTTPRAIQNPRSWQQRLGLIMHYPHSKVEVDA
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHCCHHHHHHHCCHHEECCCCCHHHHH
YIKKHVQRAFESLEREFKRRHLTVAISETDDGLQLKVDHHDEINFIYHVVSRETMPPSFM
HHHHHHHHHHHHHHHHHHHCEEEEEEEECCCCEEEEECCCCCHHHHHHHHHCCCCCCCCE
LEQEHNADVEKYFQAEVFLREGGQNYDVMDWTEEDLIQDIIDQYERHLYFLSVMRAQTGN
ECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; choline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + choline [Periplasm] = Proton [Cytoplasm] + choline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]