Click here to switch to the map view.

The map label for this gene is kef [H]

Identifier: 50086405

GI number: 50086405

Start: 3359090

End: 3360931

Strand: Reverse

Name: kef [H]

Synonym: ACIAD3441

Alternate gene names: 50086405

Gene position: 3360931-3359090 (Counterclockwise)

Preceding gene: 50086406

Following gene: 50086403

Centisome position: 93.39

GC content: 46.69

Gene sequence:

>1842_bases
ATGAGTGAAGAAGCCCATTCGATCAGCTTGTTGGCGCCTGTGGTATTACTCACTGCCGCAGTCATTGCTGTCCCATTATT
TAAACGTATTGGACTAGGGTCTGTACTGGGGTATCTGATTGCAGGTTTGGTGATTGGGCCATTTGGTCTGGCATTTTTTT
CAGATTCGGCCTCCATTCTGCATATTGCCGAACTGGGTATTGTGATGTACCTGTTTATTATTGGTCTGGAAATGCAGCCA
TCGCATTTGTGGGGGTTACGCCGTGAGATTTTTGGTCTGGGCACCTTCCAGATTGTGGTTTGTGCGCTAGGGCTTACCGC
AACAGGTATGCTGTTTGGTTTTAGCTGGCAAATTGCTTTTATTGCGGCGGCGGGTTTTGTACTGACCTCAACTGCAATTG
TGATGCAGCTTTTGGGAGATCGTGGCGATATCACGCAACCACGCGGACAAAAAATTGTAGCGATTCTTTTATTTGAAGAT
CTGCTGATTGTTCCGTTGCTGGCGATTGTGGCGTTTATGGCACCGAATCAGGTGGTTGAATCTACCACGTCGCGCTTTGA
AAATATCGGCATTGGTTTACTTGCGATTGCAGGATTGATTGCAGCAGGTTTCTGGTTGCTTAATCCACTGTTTAGATTAC
TGGCCGCATCCAAAGCGCGTGAAGTGATGACCGCAGCAGCCTTGCTGGTGGTACTGGGTGCCGCGCTGTTGATGCAGGTC
AGTGGTTTGTCGATGGCGATGGGCGCATTTCTGGCGGGTGTGTTGTTGTCTGAATCGACTTTTCGGCATCAGATTGAAGC
CGATATTGAGCCGTTCCGTGGAATTTTGCTGGGTCTATTCTTCTTGGGCGTGGGGATGTCGCTGGATTTAAAAGTGGTCG
CGCAAAACTGGCCACTAATCTTAAGTTCGGTGGTTGCCTTCATGTTTGTTAAGGCACTCATGATTTATATCGTTGCGCGT
ACCACCAAAAGCTCGCATCAAGAGGCGCTGGATCGTGCACTACTTATGGCTCAAGGGGGCGAGTTTGCATTTGTACTGTT
TGCTGCTGCGACCAGTGCGCAAGTGATTGACAGTACCATTAAATCTAACCTGACCGCGATTGTGGTGCTTTCGATGGTAC
TTACGCCGATTATTGGAATTTTATTTGGACGCTTTACCAAAAGCAGCAAGCAGGTCTCACTTGAAAATGTAGATATTGCA
GAAAACTTAAAAGGCAATGTGTTACTGATTGGCTTTGGGCGCTTTGGACAAGTGGCCAGTCAGTTACTTTTAGCGCGTGG
TGTGGATGTCACGATTATCGATACCGATATCGACATGATTCAAAACGCCGAAAAATTTGGATTTAAAATTTATTATGGTG
ATGGCTCGCGTCTGGATATTTTGCATGCATCGGGTGCAGATCAGGCCGAAGCCATTTTGGTGTGTGTCGATCAAAAAGAA
ATGACCAATAAAATTGTGGATCTGGTACAGCATGAATTTCCACTGGCCAAGCTACTGGTACGTTCTTATGACCGTGAACA
TTCGCTGTATCTGGTCAAGCAGAATGTTGATTATATTATGCGCGAAACCTTCGAGTCTGCCATTAAGTTTGGTGGTGCTG
TACTGGAAGAGCTGGGCGTAGATGAGCAAGAGATTCGACAGATTACCCGCGAAATTCGTGAGCGTGATGAAGAACGTTAT
GAAACCGAGATTGCCGCCGATGATGTATATGCAGGTGGTGGGTTGCAATATACCAATCAGCATCCGCGTCCGACTGCACC
ACTGATTCAACCCAAGCAATCTGCCAAGATCTTAAATGATAAAGATACGCAAGATCTGCTGGATAAAGACGAACAGCCTT
AA

Upstream 100 bases:

>100_bases
ATCTGAAGTATATAGGAAGCTTTGTTCTATCTGCAACGATGTGATTTCTGTCTCAGCATTGTTAAGATGAGCCTACAAAT
AAACAAAAAAAGAGATTCAT

Downstream 100 bases:

>100_bases
TGCGGTAAAGAAGTGATCCAGAAGGGTCACTTCTTGTTTTTACAGGCGATCACGCAATAAAAGCGGAGTTACTTTGAGGG
TGGGCCAAAAGCATTGGCGC

Product: glutathione-regulated potassium-efflux system protein

Products: Proton [Cytoplasm]; K (I) [Periplasm] [C]

Alternate protein names: K(+)/H(+) antiporter [H]

Number of amino acids: Translated: 613; Mature: 612

Protein sequence:

>613_residues
MSEEAHSISLLAPVVLLTAAVIAVPLFKRIGLGSVLGYLIAGLVIGPFGLAFFSDSASILHIAELGIVMYLFIIGLEMQP
SHLWGLRREIFGLGTFQIVVCALGLTATGMLFGFSWQIAFIAAAGFVLTSTAIVMQLLGDRGDITQPRGQKIVAILLFED
LLIVPLLAIVAFMAPNQVVESTTSRFENIGIGLLAIAGLIAAGFWLLNPLFRLLAASKAREVMTAAALLVVLGAALLMQV
SGLSMAMGAFLAGVLLSESTFRHQIEADIEPFRGILLGLFFLGVGMSLDLKVVAQNWPLILSSVVAFMFVKALMIYIVAR
TTKSSHQEALDRALLMAQGGEFAFVLFAAATSAQVIDSTIKSNLTAIVVLSMVLTPIIGILFGRFTKSSKQVSLENVDIA
ENLKGNVLLIGFGRFGQVASQLLLARGVDVTIIDTDIDMIQNAEKFGFKIYYGDGSRLDILHASGADQAEAILVCVDQKE
MTNKIVDLVQHEFPLAKLLVRSYDREHSLYLVKQNVDYIMRETFESAIKFGGAVLEELGVDEQEIRQITREIRERDEERY
ETEIAADDVYAGGGLQYTNQHPRPTAPLIQPKQSAKILNDKDTQDLLDKDEQP

Sequences:

>Translated_613_residues
MSEEAHSISLLAPVVLLTAAVIAVPLFKRIGLGSVLGYLIAGLVIGPFGLAFFSDSASILHIAELGIVMYLFIIGLEMQP
SHLWGLRREIFGLGTFQIVVCALGLTATGMLFGFSWQIAFIAAAGFVLTSTAIVMQLLGDRGDITQPRGQKIVAILLFED
LLIVPLLAIVAFMAPNQVVESTTSRFENIGIGLLAIAGLIAAGFWLLNPLFRLLAASKAREVMTAAALLVVLGAALLMQV
SGLSMAMGAFLAGVLLSESTFRHQIEADIEPFRGILLGLFFLGVGMSLDLKVVAQNWPLILSSVVAFMFVKALMIYIVAR
TTKSSHQEALDRALLMAQGGEFAFVLFAAATSAQVIDSTIKSNLTAIVVLSMVLTPIIGILFGRFTKSSKQVSLENVDIA
ENLKGNVLLIGFGRFGQVASQLLLARGVDVTIIDTDIDMIQNAEKFGFKIYYGDGSRLDILHASGADQAEAILVCVDQKE
MTNKIVDLVQHEFPLAKLLVRSYDREHSLYLVKQNVDYIMRETFESAIKFGGAVLEELGVDEQEIRQITREIRERDEERY
ETEIAADDVYAGGGLQYTNQHPRPTAPLIQPKQSAKILNDKDTQDLLDKDEQP
>Mature_612_residues
SEEAHSISLLAPVVLLTAAVIAVPLFKRIGLGSVLGYLIAGLVIGPFGLAFFSDSASILHIAELGIVMYLFIIGLEMQPS
HLWGLRREIFGLGTFQIVVCALGLTATGMLFGFSWQIAFIAAAGFVLTSTAIVMQLLGDRGDITQPRGQKIVAILLFEDL
LIVPLLAIVAFMAPNQVVESTTSRFENIGIGLLAIAGLIAAGFWLLNPLFRLLAASKAREVMTAAALLVVLGAALLMQVS
GLSMAMGAFLAGVLLSESTFRHQIEADIEPFRGILLGLFFLGVGMSLDLKVVAQNWPLILSSVVAFMFVKALMIYIVART
TKSSHQEALDRALLMAQGGEFAFVLFAAATSAQVIDSTIKSNLTAIVVLSMVLTPIIGILFGRFTKSSKQVSLENVDIAE
NLKGNVLLIGFGRFGQVASQLLLARGVDVTIIDTDIDMIQNAEKFGFKIYYGDGSRLDILHASGADQAEAILVCVDQKEM
TNKIVDLVQHEFPLAKLLVRSYDREHSLYLVKQNVDYIMRETFESAIKFGGAVLEELGVDEQEIRQITREIRERDEERYE
TEIAADDVYAGGGLQYTNQHPRPTAPLIQPKQSAKILNDKDTQDLLDKDEQP

Specific function: Transport system that facilitate potassium-efflux, possibly by potassium-proton antiport [H]

COG id: COG0475

COG function: function code P; Kef-type K+ transport systems, membrane components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RCK N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI157388921, Length=380, Percent_Identity=26.5789473684211, Blast_Score=78, Evalue=3e-14,
Organism=Escherichia coli, GI1786232, Length=545, Percent_Identity=40.7339449541284, Blast_Score=418, Evalue=1e-118,
Organism=Escherichia coli, GI1789749, Length=551, Percent_Identity=41.923774954628, Blast_Score=402, Evalue=1e-113,
Organism=Escherichia coli, GI1786685, Length=510, Percent_Identity=24.9019607843137, Blast_Score=145, Evalue=1e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006153
- InterPro:   IPR004771
- InterPro:   IPR016040
- InterPro:   IPR003148 [H]

Pfam domain/function: PF00999 Na_H_Exchanger; PF02254 TrkA_N [H]

EC number: NA

Molecular weight: Translated: 66666; Mature: 66535

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS00664 VINCULIN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEEAHSISLLAPVVLLTAAVIAVPLFKRIGLGSVLGYLIAGLVIGPFGLAFFSDSASIL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
HIAELGIVMYLFIIGLEMQPSHLWGLRREIFGLGTFQIVVCALGLTATGMLFGFSWQIAF
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
IAAAGFVLTSTAIVMQLLGDRGDITQPRGQKIVAILLFEDLLIVPLLAIVAFMAPNQVVE
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCHHHHH
STTSRFENIGIGLLAIAGLIAAGFWLLNPLFRLLAASKAREVMTAAALLVVLGAALLMQV
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGLSMAMGAFLAGVLLSESTFRHQIEADIEPFRGILLGLFFLGVGMSLDLKVVAQNWPLI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHH
LSSVVAFMFVKALMIYIVARTTKSSHQEALDRALLMAQGGEFAFVLFAAATSAQVIDSTI
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHH
KSNLTAIVVLSMVLTPIIGILFGRFTKSSKQVSLENVDIAENLKGNVLLIGFGRFGQVAS
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCHHHCCCCCEEEEECCHHHHHHH
QLLLARGVDVTIIDTDIDMIQNAEKFGFKIYYGDGSRLDILHASGADQAEAILVCVDQKE
HHHHHCCCCEEEEECCHHHHHCHHHCCEEEEECCCCEEEEEECCCCCCCCEEEEEECCHH
MTNKIVDLVQHEFPLAKLLVRSYDREHSLYLVKQNVDYIMRETFESAIKFGGAVLEELGV
HHHHHHHHHHHCCHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCC
DEQEIRQITREIRERDEERYETEIAADDVYAGGGLQYTNQHPRPTAPLIQPKQSAKILND
CHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCCCCCCCCCCCCCCCCCHHHHCCCC
KDTQDLLDKDEQP
CCHHHHHCCCCCC
>Mature Secondary Structure 
SEEAHSISLLAPVVLLTAAVIAVPLFKRIGLGSVLGYLIAGLVIGPFGLAFFSDSASIL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
HIAELGIVMYLFIIGLEMQPSHLWGLRREIFGLGTFQIVVCALGLTATGMLFGFSWQIAF
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
IAAAGFVLTSTAIVMQLLGDRGDITQPRGQKIVAILLFEDLLIVPLLAIVAFMAPNQVVE
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCHHHHH
STTSRFENIGIGLLAIAGLIAAGFWLLNPLFRLLAASKAREVMTAAALLVVLGAALLMQV
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGLSMAMGAFLAGVLLSESTFRHQIEADIEPFRGILLGLFFLGVGMSLDLKVVAQNWPLI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHH
LSSVVAFMFVKALMIYIVARTTKSSHQEALDRALLMAQGGEFAFVLFAAATSAQVIDSTI
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHH
KSNLTAIVVLSMVLTPIIGILFGRFTKSSKQVSLENVDIAENLKGNVLLIGFGRFGQVAS
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCHHHCCCCCEEEEECCHHHHHHH
QLLLARGVDVTIIDTDIDMIQNAEKFGFKIYYGDGSRLDILHASGADQAEAILVCVDQKE
HHHHHCCCCEEEEECCHHHHHCHHHCCEEEEECCCCEEEEEECCCCCCCCEEEEEECCHH
MTNKIVDLVQHEFPLAKLLVRSYDREHSLYLVKQNVDYIMRETFESAIKFGGAVLEELGV
HHHHHHHHHHHCCHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCC
DEQEIRQITREIRERDEERYETEIAADDVYAGGGLQYTNQHPRPTAPLIQPKQSAKILND
CHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCCCCCCCCCCCCCCCCCHHHHCCCC
KDTQDLLDKDEQP
CCHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; K (I) [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + K (I) [Cytoplasm] = Proton [Cytoplasm] + K (I) [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]