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The map label for this gene is hsdS [H]

Identifier: 50086399

GI number: 50086399

Start: 3350154

End: 3351500

Strand: Reverse

Name: hsdS [H]

Synonym: ACIAD3431

Alternate gene names: 50086399

Gene position: 3351500-3350154 (Counterclockwise)

Preceding gene: 50086400

Following gene: 50086398

Centisome position: 93.13

GC content: 32.67

Gene sequence:

>1347_bases
ATGAGTCAGTTACCATGTTACGAAAGTTATAAGAATAGTGGCGTGCAATGGTTAGGGGAAATCCCTAGCCATTGGGAAGT
AAAACGCATGAAGTTTTTACTATCAGAAAAGTTAAAGTATGGAGCTAATGAATCTGCTGAATCTGAGGATAAAGATCAAC
CTAGATATATCCGAATTACAGATATCAATGACTCAGGTACTTTACGTGAAGATACATTTAAATCTTTAGAGATTGAAAAA
GCACAAGAATATCTACTGAATGACTTAGATATTTTACTTGCAAGAAGTGGGGCAACTGTTGGTAAAAGTTACTTGCATAA
AAAGGACAAAGTTAATGTGGCTTGTTATGCAGGTTATTTAATTCGAGCTAGATTCAATAAAGAAAATTATGATCCTCAAT
TCATAAATCTTTTTTTGCAGTCTAAGGCTTATTGGAGTTGGATTGAATCTGTAAATATTCAAGCTACTATTCAGAATGTA
AGCGCTGAAAAATATAATGATTTGGCTTTATCTATTCCGTCATTAGCAGAACAAAAAATCATTGCAGATTTTTTAGATAA
ACGCCTTGCCCAAGTGGATGCTTTAATCGCTAAGCAAGAAACCTTATTGGAAAAATTGGCTGAACAACGTGTAGCTTTAA
TTTCTCATGCGGTGACTAAGGGTTTAAATCCTGATGTAGAGATGAAAGAGAGTGACGTTGTACTTTTGGGTAATATTCCA
AATACATGGAATATTAAACGGCTTAAATTTCTATTATCTGAAAAATTGAAGTATGGAGCAAATGAATCAGCTGAATCAGA
GGATAAAGAAAATCCACGATATATACGAATCACTGATATTGATGATTCTGGAAATTTGAAGGATGAAACATTTAAATCTT
TAGAGTCAGAAAAAGCACAAGAATATCTATTGGATGACTTAGATATTTTACTAGCAAGAAGTGGGGCAACAGTTGGTAAA
AGTTATCTTTACAAAGCTGAATCAGTAGGTATTGCCTGTTATGCGGGTTATCTAATTCGTGCTAGGTTAGATCAGGAAAA
TTATAATCCTGAATTTGTTAATTATTTTCTTCAATCTAAACAATATTGGGATTGGATTAGTTCCATTAATATCCAAGCTA
CGATTCAGAATGTAAGTGCTGAAAAGTATAATGATTTAACTTTGGCTATTCCTTCCTTGGAAGAGCAAAAGCAATTAATT
GAATATTTAAAAAATGAAGATGAAAAGTTTAATAGAGCTATTAGTAAAGGCAAAAAACTCGTTCATCTTTTAAATGAATA
TCGCTCAACCTTAATCACGCAAGTGGTGACAGGGAAGATCGATGTTCAAAATCTTAAAGTAAACTAA

Upstream 100 bases:

>100_bases
ATTTTTATATTTATGAGCCACCACGTCCATTGGAAGAAATTAAGGCTGAGATTGTTCAGCTTGAGCAGGAGATTATGCAG
ATGTTAGGAGGTTTGTCTGC

Downstream 100 bases:

>100_bases
AAAACATTGGGTCATATTTTTTGGGGGGGAATGCCATGCATAGCGAATATTATTTTGAACAAGCCATCGAACAAAGCTTA
ACAACGGCTGGCGGTTATGT

Product: type I restriction-modification system specificity determinant for hsdM and hsdR (HsdS)

Products: NA

Alternate protein names: S.StySPI; Type I restriction enzyme StySPI specificity protein; S protein [H]

Number of amino acids: Translated: 448; Mature: 447

Protein sequence:

>448_residues
MSQLPCYESYKNSGVQWLGEIPSHWEVKRMKFLLSEKLKYGANESAESEDKDQPRYIRITDINDSGTLREDTFKSLEIEK
AQEYLLNDLDILLARSGATVGKSYLHKKDKVNVACYAGYLIRARFNKENYDPQFINLFLQSKAYWSWIESVNIQATIQNV
SAEKYNDLALSIPSLAEQKIIADFLDKRLAQVDALIAKQETLLEKLAEQRVALISHAVTKGLNPDVEMKESDVVLLGNIP
NTWNIKRLKFLLSEKLKYGANESAESEDKENPRYIRITDIDDSGNLKDETFKSLESEKAQEYLLDDLDILLARSGATVGK
SYLYKAESVGIACYAGYLIRARLDQENYNPEFVNYFLQSKQYWDWISSINIQATIQNVSAEKYNDLTLAIPSLEEQKQLI
EYLKNEDEKFNRAISKGKKLVHLLNEYRSTLITQVVTGKIDVQNLKVN

Sequences:

>Translated_448_residues
MSQLPCYESYKNSGVQWLGEIPSHWEVKRMKFLLSEKLKYGANESAESEDKDQPRYIRITDINDSGTLREDTFKSLEIEK
AQEYLLNDLDILLARSGATVGKSYLHKKDKVNVACYAGYLIRARFNKENYDPQFINLFLQSKAYWSWIESVNIQATIQNV
SAEKYNDLALSIPSLAEQKIIADFLDKRLAQVDALIAKQETLLEKLAEQRVALISHAVTKGLNPDVEMKESDVVLLGNIP
NTWNIKRLKFLLSEKLKYGANESAESEDKENPRYIRITDIDDSGNLKDETFKSLESEKAQEYLLDDLDILLARSGATVGK
SYLYKAESVGIACYAGYLIRARLDQENYNPEFVNYFLQSKQYWDWISSINIQATIQNVSAEKYNDLTLAIPSLEEQKQLI
EYLKNEDEKFNRAISKGKKLVHLLNEYRSTLITQVVTGKIDVQNLKVN
>Mature_447_residues
SQLPCYESYKNSGVQWLGEIPSHWEVKRMKFLLSEKLKYGANESAESEDKDQPRYIRITDINDSGTLREDTFKSLEIEKA
QEYLLNDLDILLARSGATVGKSYLHKKDKVNVACYAGYLIRARFNKENYDPQFINLFLQSKAYWSWIESVNIQATIQNVS
AEKYNDLALSIPSLAEQKIIADFLDKRLAQVDALIAKQETLLEKLAEQRVALISHAVTKGLNPDVEMKESDVVLLGNIPN
TWNIKRLKFLLSEKLKYGANESAESEDKENPRYIRITDIDDSGNLKDETFKSLESEKAQEYLLDDLDILLARSGATVGKS
YLYKAESVGIACYAGYLIRARLDQENYNPEFVNYFLQSKQYWDWISSINIQATIQNVSAEKYNDLTLAIPSLEEQKQLIE
YLKNEDEKFNRAISKGKKLVHLLNEYRSTLITQVVTGKIDVQNLKVN

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to

COG id: COG0732

COG function: function code V; Restriction endonuclease S subunits

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type-I restriction system S methylase family [H]

Homologues:

Organism=Escherichia coli, GI1790807, Length=366, Percent_Identity=26.5027322404372, Blast_Score=81, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000055 [H]

Pfam domain/function: PF01420 Methylase_S [H]

EC number: NA

Molecular weight: Translated: 51383; Mature: 51252

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQLPCYESYKNSGVQWLGEIPSHWEVKRMKFLLSEKLKYGANESAESEDKDQPRYIRIT
CCCCCCHHHHHCCCCHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEE
DINDSGTLREDTFKSLEIEKAQEYLLNDLDILLARSGATVGKSYLHKKDKVNVACYAGYL
ECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCEEEEEEEE
IRARFNKENYDPQFINLFLQSKAYWSWIESVNIQATIQNVSAEKYNDLALSIPSLAEQKI
EEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHCCCEEECHHHHHHHH
IADFLDKRLAQVDALIAKQETLLEKLAEQRVALISHAVTKGLNPDVEMKESDVVLLGNIP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCC
NTWNIKRLKFLLSEKLKYGANESAESEDKENPRYIRITDIDDSGNLKDETFKSLESEKAQ
CCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHH
EYLLDDLDILLARSGATVGKSYLYKAESVGIACYAGYLIRARLDQENYNPEFVNYFLQSK
HHHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHEEECCCCCCHHHHHHHHHCC
QYWDWISSINIQATIQNVSAEKYNDLTLAIPSLEEQKQLIEYLKNEDEKFNRAISKGKKL
HHHHHHHCCCEEEEEECCCCCCCCCEEEECCCHHHHHHHHHHHHCCHHHHHHHHHHHHHH
VHLLNEYRSTLITQVVTGKIDVQNLKVN
HHHHHHHHHHHHHHHHHCCEEEEEEECC
>Mature Secondary Structure 
SQLPCYESYKNSGVQWLGEIPSHWEVKRMKFLLSEKLKYGANESAESEDKDQPRYIRIT
CCCCCHHHHHCCCCHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEE
DINDSGTLREDTFKSLEIEKAQEYLLNDLDILLARSGATVGKSYLHKKDKVNVACYAGYL
ECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCEEEEEEEE
IRARFNKENYDPQFINLFLQSKAYWSWIESVNIQATIQNVSAEKYNDLALSIPSLAEQKI
EEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHCCCEEECHHHHHHHH
IADFLDKRLAQVDALIAKQETLLEKLAEQRVALISHAVTKGLNPDVEMKESDVVLLGNIP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCC
NTWNIKRLKFLLSEKLKYGANESAESEDKENPRYIRITDIDDSGNLKDETFKSLESEKAQ
CCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHH
EYLLDDLDILLARSGATVGKSYLYKAESVGIACYAGYLIRARLDQENYNPEFVNYFLQSK
HHHHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHEEECCCCCCHHHHHHHHHCC
QYWDWISSINIQATIQNVSAEKYNDLTLAIPSLEEQKQLIEYLKNEDEKFNRAISKGKKL
HHHHHHHCCCEEEEEECCCCCCCCCEEEECCCHHHHHHHHHHHHCCHHHHHHHHHHHHHH
VHLLNEYRSTLITQVVTGKIDVQNLKVN
HHHHHHHHHHHHHHHHHCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3025838 [H]