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The map label for this gene is poxB [H]

Identifier: 50086357

GI number: 50086357

Start: 3291936

End: 3293657

Strand: Reverse

Name: poxB [H]

Synonym: ACIAD3381

Alternate gene names: 50086357

Gene position: 3293657-3291936 (Counterclockwise)

Preceding gene: 50086360

Following gene: 50086356

Centisome position: 91.53

GC content: 45.3

Gene sequence:

>1722_bases
ATGAGTAATCAAAAAGTATCCGATATTATCATCGAAGTGCTTGAGCAAGCAGGCGTTCAGCGCTGTTACGGTATTGTGGG
TGACACCTTAAACCATGTCACCGACTCCATGTCAAAAAGCAAGATTGAATGGATTCATGTGCGCCATGAAGAAGTTGGTG
GCTTTGCAGCAGGTACAGATGCATTACTCAGTGGTCACTTAACCGCATGTGCTGGTTCTTGCGGGCCGGGCAGTCTGCAC
TTTATTAATGGCTTGTATGAATCACACCGAAATCGTGCGCCCGTTATTTTGATTGCGAGCCAGATTTCGACCGAAATGGC
AGGATTTATCGACTTTCCGCAATACGTCGATTTTAAATCGGTTTATGCGAAAAATTCGGTCTTCTGTGAAGAAATTACTC
AACCCTCACAAGCTAGACATATCATGAGCATGGCCTGTCAGGCAGCACTCAATAAACGCGGTGTCGCTGTGGTCATCGTG
CCAGCCAATATCAGTGAAGCATCGGCTGAAGCGGGTCTACCTTTCGTGCCACGTCATGTTGAACCTGATATTTTGCCAAA
CAAAGCTGAACTGCATCAAATGGTTGAGCTGATTTCCCAGCATCAAAAAATTGGAATTTATGCAGGTGCGGGGTGTGAAG
GCGCACATGATCAGTTAATTGCGTTTGCTGAAAAGTTAAAAGCCCCTGTGGCGCATACCTCACGTGCCAAAGATTTTGTG
GAGTACGATAACCCATACAACATGGGCATGACGGGTATTTTTGGCAATAAAGCAGGTTATCACACGCTCATGGATTGTGA
TTTACTGATTTTGCTGGGTGCCGATTTTGCATGGGCACAGTACTATCCAAGTCATGCCAAAATCTTGCAGATTGATATTG
ATCCGACACATTTAGGGCGACGTCACCCAATTACACTGGGTGCGGTAGGTAAAATCTCATCGACGCTCGATGCCTTATTA
CCATTGCTTGAAACGCGTCAAGAACGCGCATTTTTAGATCATTGTCTTGAACTGAAACATCACAGTGATGAAACACGGCA
TAAAGAAGAACGTGTCGGAAAAGACGGGCTGATTCATCCACAGTATCTGGTGTCGTTGCTTAACCGTTATGCCGATCAGG
ATGCAATTTTCTTTGGTGATGGGGGCTCACCGATGGTGTGGGTACTCAGACATATCGATGTGAATGGCAAACGTCGAACC
TTTACCAGTTTGTTGCATGGCACGATGGCCAATGCCATGCCCCAGGCGCTTGGCGCACAAAAAGCTTTTCCGAATCGCCA
GATTATTGCGCTGTGTGGCGATGGTGGATTAGCCATGTTACTTGGTGATTTACTCACTACGATTCAGGAAAAATTACCGA
TTAAAATTGTGGTATTTAACAACAGCTCGCTTAATTTTGTTGAGCTTGAACAAAAAGTCGAAGGCTTGCTTGATCATTAT
ACCGACTTGCTCAATCCTGATTTTGGCAAGCTTGCCAGTGTGATCGGGCTACATGGGCAGACTGTGACGCATGGCGATGG
CTTGGAGCAGGCAGTTGAAAACTTCTTAAAGCATGATGGTCCAGCATTACTCAATGTGCATACCAATCCGATGGAACTGG
TGATGCCGCCAGATCCGAATCTGAATCAAGTCTCGTCCACTTCACTTTATGCAATTAAGGCCTTGATGTCGGGTCGAGTA
GATGACGTTAAAAATTTGTTGGTCAATAATTTCATTAAATAA

Upstream 100 bases:

>100_bases
AGAACGCCACAAAAACTTACTGGCAGTCTGGGGCTAGCAAGACCACTATAAAAAACAAAAGTAATTCAAATCATGATAAA
AAAGCTGGAGAATTGACCTA

Downstream 100 bases:

>100_bases
ATTTAATGAACACAAGTCATCTTAGGGCACAACTATAAGTTAAAAATGCATGATCTAGCGTCATGCATTTTTTATATCAG
GAAACAATTTTGACAACATT

Product: pyruvate dehydrogenase (cytochrome)

Products: NA

Alternate protein names: Pyruvate oxidase; POX [H]

Number of amino acids: Translated: 573; Mature: 572

Protein sequence:

>573_residues
MSNQKVSDIIIEVLEQAGVQRCYGIVGDTLNHVTDSMSKSKIEWIHVRHEEVGGFAAGTDALLSGHLTACAGSCGPGSLH
FINGLYESHRNRAPVILIASQISTEMAGFIDFPQYVDFKSVYAKNSVFCEEITQPSQARHIMSMACQAALNKRGVAVVIV
PANISEASAEAGLPFVPRHVEPDILPNKAELHQMVELISQHQKIGIYAGAGCEGAHDQLIAFAEKLKAPVAHTSRAKDFV
EYDNPYNMGMTGIFGNKAGYHTLMDCDLLILLGADFAWAQYYPSHAKILQIDIDPTHLGRRHPITLGAVGKISSTLDALL
PLLETRQERAFLDHCLELKHHSDETRHKEERVGKDGLIHPQYLVSLLNRYADQDAIFFGDGGSPMVWVLRHIDVNGKRRT
FTSLLHGTMANAMPQALGAQKAFPNRQIIALCGDGGLAMLLGDLLTTIQEKLPIKIVVFNNSSLNFVELEQKVEGLLDHY
TDLLNPDFGKLASVIGLHGQTVTHGDGLEQAVENFLKHDGPALLNVHTNPMELVMPPDPNLNQVSSTSLYAIKALMSGRV
DDVKNLLVNNFIK

Sequences:

>Translated_573_residues
MSNQKVSDIIIEVLEQAGVQRCYGIVGDTLNHVTDSMSKSKIEWIHVRHEEVGGFAAGTDALLSGHLTACAGSCGPGSLH
FINGLYESHRNRAPVILIASQISTEMAGFIDFPQYVDFKSVYAKNSVFCEEITQPSQARHIMSMACQAALNKRGVAVVIV
PANISEASAEAGLPFVPRHVEPDILPNKAELHQMVELISQHQKIGIYAGAGCEGAHDQLIAFAEKLKAPVAHTSRAKDFV
EYDNPYNMGMTGIFGNKAGYHTLMDCDLLILLGADFAWAQYYPSHAKILQIDIDPTHLGRRHPITLGAVGKISSTLDALL
PLLETRQERAFLDHCLELKHHSDETRHKEERVGKDGLIHPQYLVSLLNRYADQDAIFFGDGGSPMVWVLRHIDVNGKRRT
FTSLLHGTMANAMPQALGAQKAFPNRQIIALCGDGGLAMLLGDLLTTIQEKLPIKIVVFNNSSLNFVELEQKVEGLLDHY
TDLLNPDFGKLASVIGLHGQTVTHGDGLEQAVENFLKHDGPALLNVHTNPMELVMPPDPNLNQVSSTSLYAIKALMSGRV
DDVKNLLVNNFIK
>Mature_572_residues
SNQKVSDIIIEVLEQAGVQRCYGIVGDTLNHVTDSMSKSKIEWIHVRHEEVGGFAAGTDALLSGHLTACAGSCGPGSLHF
INGLYESHRNRAPVILIASQISTEMAGFIDFPQYVDFKSVYAKNSVFCEEITQPSQARHIMSMACQAALNKRGVAVVIVP
ANISEASAEAGLPFVPRHVEPDILPNKAELHQMVELISQHQKIGIYAGAGCEGAHDQLIAFAEKLKAPVAHTSRAKDFVE
YDNPYNMGMTGIFGNKAGYHTLMDCDLLILLGADFAWAQYYPSHAKILQIDIDPTHLGRRHPITLGAVGKISSTLDALLP
LLETRQERAFLDHCLELKHHSDETRHKEERVGKDGLIHPQYLVSLLNRYADQDAIFFGDGGSPMVWVLRHIDVNGKRRTF
TSLLHGTMANAMPQALGAQKAFPNRQIIALCGDGGLAMLLGDLLTTIQEKLPIKIVVFNNSSLNFVELEQKVEGLLDHYT
DLLNPDFGKLASVIGLHGQTVTHGDGLEQAVENFLKHDGPALLNVHTNPMELVMPPDPNLNQVSSTSLYAIKALMSGRVD
DVKNLLVNNFIK

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=568, Percent_Identity=22.1830985915493, Blast_Score=90, Evalue=6e-18,
Organism=Escherichia coli, GI1787096, Length=580, Percent_Identity=43.2758620689655, Blast_Score=486, Evalue=1e-138,
Organism=Escherichia coli, GI1790104, Length=550, Percent_Identity=26.9090909090909, Blast_Score=166, Evalue=3e-42,
Organism=Escherichia coli, GI87081685, Length=533, Percent_Identity=24.202626641651, Blast_Score=149, Evalue=5e-37,
Organism=Escherichia coli, GI1786717, Length=496, Percent_Identity=24.1935483870968, Blast_Score=119, Evalue=5e-28,
Organism=Escherichia coli, GI1788716, Length=560, Percent_Identity=24.1071428571429, Blast_Score=107, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17531301, Length=554, Percent_Identity=23.4657039711191, Blast_Score=108, Evalue=9e-24,
Organism=Caenorhabditis elegans, GI17531299, Length=554, Percent_Identity=23.4657039711191, Blast_Score=108, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6323755, Length=565, Percent_Identity=22.4778761061947, Blast_Score=122, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6320816, Length=505, Percent_Identity=25.1485148514851, Blast_Score=104, Evalue=5e-23,
Organism=Drosophila melanogaster, GI19922626, Length=569, Percent_Identity=23.1985940246046, Blast_Score=105, Evalue=8e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =1.2.5.1 [H]

Molecular weight: Translated: 62772; Mature: 62641

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNQKVSDIIIEVLEQAGVQRCYGIVGDTLNHVTDSMSKSKIEWIHVRHEEVGGFAAGTD
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEHHHCCCCCCCCH
ALLSGHLTACAGSCGPGSLHFINGLYESHRNRAPVILIASQISTEMAGFIDFPQYVDFKS
HHHHCCHHHHCCCCCCCCHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHCCCCHHCCHHH
VYAKNSVFCEEITQPSQARHIMSMACQAALNKRGVAVVIVPANISEASAEAGLPFVPRHV
HHHCCCHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHCCCCCCCCCCC
EPDILPNKAELHQMVELISQHQKIGIYAGAGCEGAHDQLIAFAEKLKAPVAHTSRAKDFV
CCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHH
EYDNPYNMGMTGIFGNKAGYHTLMDCDLLILLGADFAWAQYYPSHAKILQIDIDPTHLGR
HCCCCCCCCCEEECCCCCCCCHHHHCCEEEEECCCHHHHHHCCCCCEEEEEECCHHHCCC
RHPITLGAVGKISSTLDALLPLLETRQERAFLDHCLELKHHSDETRHKEERVGKDGLIHP
CCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCH
QYLVSLLNRYADQDAIFFGDGGSPMVWVLRHIDVNGKRRTFTSLLHGTMANAMPQALGAQ
HHHHHHHHHHCCCCEEEECCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHH
KAFPNRQIIALCGDGGLAMLLGDLLTTIQEKLPIKIVVFNNSSLNFVELEQKVEGLLDHY
HCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHH
TDLLNPDFGKLASVIGLHGQTVTHGDGLEQAVENFLKHDGPALLNVHTNPMELVMPPDPN
HHHCCCCHHHHHHHHHCCCCEEECCCCHHHHHHHHHHCCCCEEEEEECCCEEEEECCCCC
LNQVSSTSLYAIKALMSGRVDDVKNLLVNNFIK
CCCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
SNQKVSDIIIEVLEQAGVQRCYGIVGDTLNHVTDSMSKSKIEWIHVRHEEVGGFAAGTD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEHHHCCCCCCCCH
ALLSGHLTACAGSCGPGSLHFINGLYESHRNRAPVILIASQISTEMAGFIDFPQYVDFKS
HHHHCCHHHHCCCCCCCCHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHCCCCHHCCHHH
VYAKNSVFCEEITQPSQARHIMSMACQAALNKRGVAVVIVPANISEASAEAGLPFVPRHV
HHHCCCHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHCCCCCCCCCCC
EPDILPNKAELHQMVELISQHQKIGIYAGAGCEGAHDQLIAFAEKLKAPVAHTSRAKDFV
CCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHH
EYDNPYNMGMTGIFGNKAGYHTLMDCDLLILLGADFAWAQYYPSHAKILQIDIDPTHLGR
HCCCCCCCCCEEECCCCCCCCHHHHCCEEEEECCCHHHHHHCCCCCEEEEEECCHHHCCC
RHPITLGAVGKISSTLDALLPLLETRQERAFLDHCLELKHHSDETRHKEERVGKDGLIHP
CCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCH
QYLVSLLNRYADQDAIFFGDGGSPMVWVLRHIDVNGKRRTFTSLLHGTMANAMPQALGAQ
HHHHHHHHHHCCCCEEEECCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHH
KAFPNRQIIALCGDGGLAMLLGDLLTTIQEKLPIKIVVFNNSSLNFVELEQKVEGLLDHY
HCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHH
TDLLNPDFGKLASVIGLHGQTVTHGDGLEQAVENFLKHDGPALLNVHTNPMELVMPPDPN
HHHCCCCHHHHHHHHHCCCCEEECCCCHHHHHHHHHHCCCCEEEEEECCCEEEEECCCCC
LNQVSSTSLYAIKALMSGRVDDVKNLLVNNFIK
CCCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3016647; 8905232; 9278503; 2663858; 3902830; 8022274 [H]