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The map label for this gene is gltX

Identifier: 50086349

GI number: 50086349

Start: 3280430

End: 3281938

Strand: Direct

Name: gltX

Synonym: ACIAD3371

Alternate gene names: 50086349

Gene position: 3280430-3281938 (Clockwise)

Preceding gene: 50086348

Following gene: 50086350

Centisome position: 91.16

GC content: 44.67

Gene sequence:

>1509_bases
ATGAAAGTCCGTACGCGTATTGCACCTTCTCCGACTGGTTTTCCTCATGTAGGTACTGCTTATATCGCTCTGTTTAATCT
GTGCTTTGCCAAACAGCATGGCGGTGAGTTTATTTTACGTATTGAAGATACCGATCAGCTTCGTTCGACCCCTGAATCAG
AACAAATGATTCTGGATTCATTGCGCTGGTTAGGTCTGAACTGGTCGGAAGGTCCTGATGTGGGTGGTCCACATGCACCT
TACCGTCAGTCAGAGCGCATGGGTATTTATAAACAGTATGCACTTGATCTGATTGAGAAAGGCCATGCCTTTTATTGCTT
TGCCACAGCCGAAGAGCTGGATCAAATGCGTGCAGAACAGCAGGCGCGTGGTGAAACACCAAAGTACGATGGCCGAGGTT
TAAAACTTTCACAAGAAGAAGTTGAACGTCGTCTGGCTGCGGGTGAACCGCATGTGATTCGTATGAAAGTTCCTGAAGAA
GGGATTTGTAAATTCAACGATTTACTCCGCGGTGAAGTCGAAATTCCATGGGCACAAGTCGATATGCAAGTCTTGCTTAA
AACAGACGGCTTACCCACCTATCATTTGGCCAATGTGGTCGATGACCACTTAATGGAAATCACGCATGTTTTGCGTGGTG
AAGAATGGTTACCCTCTGCACCAAAGCATCAATTACTGTATCAATATTTCGGCTGGGACATGCCAACGCTATGTCATATG
CCATTATTGCGTAATCCAGACAAATCAAAACTGTCAAAGCGTAAAAATCCGACTTCCATCAATTATTACCGCGACATTGG
TGTACTTCCAGAAGCCCTGCTGAATTACTTGGGCCGTATGGGTTGGTCAATGCCAGATGAACGTGAAGTGTTTACCTTGA
CTGATATGATTGAGCATTTTGATATTCAACGTGTATCGTTGGGCGGTCCAATCTTTGATGTCGACAAATTGAACTGGCTC
AATGGTCAATGGATCAAATCGTTGACACCAGGTCAGTTACTTGATCGCCTACTCAGCTGGAAAAGTGACCGTCAAACACT
GGAAGATATTGCCACTGCGATTCAACCTCGCATCAATCTGCTTTCAGAAGCAGTCAATTGGGCGGGATTTTATTTCAACC
ATATGCCAAATATTACGGCAGAAATGTTTGAAAGCAAAAAACTCAGCCCTGAACAGGTTCGTCAAAGCTTACAGTTTGCA
ATCTGGCGTCTGGAAAGCCAATTTACTTGGAATAACGAGACGGTGAGCCAGATTCTTATGGATCTTGCCAATCAGATGGA
CATCAAACTCCGTGATTTCATGCCAGCGTTCTTTATTGCGATTGCAGGTTCAACCAGCTCAACGCCTGTCATGCAATCAA
TGGTCACTATCGGCCCAGACTTAACCTTTGCCCGTTTGCGTCGTGCGCTTGAAATTGTGGGTGCACCAAGCAAAAAAGAG
CTTAAAGTGTGGGAAAAACTCAACGAATCGTTGAAATTGCCGAAAAATGAAACAGTTGACAACACATAA

Upstream 100 bases:

>100_bases
AGCTTCTTAAAAGCCTGATCTCTTGCCAAAATAAAAGGTCTAGATATCAGGCTTTTTCCGTTATACTAGATCTCAATTTT
TATCTTCACTTTTAGGCCCT

Downstream 100 bases:

>100_bases
TTCATTTTAGTGTTGACGTGTGAGTACAATGCCCCTAATATTGCGCTCACACAGACTGGGGTCATAGCTCAGTTGGTAGA
GCGCTACAATGGCATTGTAG

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS

Number of amino acids: Translated: 502; Mature: 502

Protein sequence:

>502_residues
MKVRTRIAPSPTGFPHVGTAYIALFNLCFAKQHGGEFILRIEDTDQLRSTPESEQMILDSLRWLGLNWSEGPDVGGPHAP
YRQSERMGIYKQYALDLIEKGHAFYCFATAEELDQMRAEQQARGETPKYDGRGLKLSQEEVERRLAAGEPHVIRMKVPEE
GICKFNDLLRGEVEIPWAQVDMQVLLKTDGLPTYHLANVVDDHLMEITHVLRGEEWLPSAPKHQLLYQYFGWDMPTLCHM
PLLRNPDKSKLSKRKNPTSINYYRDIGVLPEALLNYLGRMGWSMPDEREVFTLTDMIEHFDIQRVSLGGPIFDVDKLNWL
NGQWIKSLTPGQLLDRLLSWKSDRQTLEDIATAIQPRINLLSEAVNWAGFYFNHMPNITAEMFESKKLSPEQVRQSLQFA
IWRLESQFTWNNETVSQILMDLANQMDIKLRDFMPAFFIAIAGSTSSTPVMQSMVTIGPDLTFARLRRALEIVGAPSKKE
LKVWEKLNESLKLPKNETVDNT

Sequences:

>Translated_502_residues
MKVRTRIAPSPTGFPHVGTAYIALFNLCFAKQHGGEFILRIEDTDQLRSTPESEQMILDSLRWLGLNWSEGPDVGGPHAP
YRQSERMGIYKQYALDLIEKGHAFYCFATAEELDQMRAEQQARGETPKYDGRGLKLSQEEVERRLAAGEPHVIRMKVPEE
GICKFNDLLRGEVEIPWAQVDMQVLLKTDGLPTYHLANVVDDHLMEITHVLRGEEWLPSAPKHQLLYQYFGWDMPTLCHM
PLLRNPDKSKLSKRKNPTSINYYRDIGVLPEALLNYLGRMGWSMPDEREVFTLTDMIEHFDIQRVSLGGPIFDVDKLNWL
NGQWIKSLTPGQLLDRLLSWKSDRQTLEDIATAIQPRINLLSEAVNWAGFYFNHMPNITAEMFESKKLSPEQVRQSLQFA
IWRLESQFTWNNETVSQILMDLANQMDIKLRDFMPAFFIAIAGSTSSTPVMQSMVTIGPDLTFARLRRALEIVGAPSKKE
LKVWEKLNESLKLPKNETVDNT
>Mature_502_residues
MKVRTRIAPSPTGFPHVGTAYIALFNLCFAKQHGGEFILRIEDTDQLRSTPESEQMILDSLRWLGLNWSEGPDVGGPHAP
YRQSERMGIYKQYALDLIEKGHAFYCFATAEELDQMRAEQQARGETPKYDGRGLKLSQEEVERRLAAGEPHVIRMKVPEE
GICKFNDLLRGEVEIPWAQVDMQVLLKTDGLPTYHLANVVDDHLMEITHVLRGEEWLPSAPKHQLLYQYFGWDMPTLCHM
PLLRNPDKSKLSKRKNPTSINYYRDIGVLPEALLNYLGRMGWSMPDEREVFTLTDMIEHFDIQRVSLGGPIFDVDKLNWL
NGQWIKSLTPGQLLDRLLSWKSDRQTLEDIATAIQPRINLLSEAVNWAGFYFNHMPNITAEMFESKKLSPEQVRQSLQFA
IWRLESQFTWNNETVSQILMDLANQMDIKLRDFMPAFFIAIAGSTSSTPVMQSMVTIGPDLTFARLRRALEIVGAPSKKE
LKVWEKLNESLKLPKNETVDNT

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=491, Percent_Identity=34.0122199592668, Blast_Score=263, Evalue=2e-70,
Organism=Escherichia coli, GI1788743, Length=482, Percent_Identity=35.6846473029046, Blast_Score=309, Evalue=3e-85,
Organism=Escherichia coli, GI226510925, Length=259, Percent_Identity=33.5907335907336, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI1786895, Length=146, Percent_Identity=27.3972602739726, Blast_Score=63, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI71993394, Length=487, Percent_Identity=34.4969199178645, Blast_Score=266, Evalue=2e-71,
Organism=Saccharomyces cerevisiae, GI6324540, Length=371, Percent_Identity=39.622641509434, Blast_Score=258, Evalue=2e-69,
Organism=Saccharomyces cerevisiae, GI6324742, Length=304, Percent_Identity=25.6578947368421, Blast_Score=74, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6321192, Length=264, Percent_Identity=24.2424242424242, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24665321, Length=494, Percent_Identity=32.9959514170041, Blast_Score=268, Evalue=5e-72,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE_ACIAD (Q6F7C8)

Other databases:

- EMBL:   CR543861
- RefSeq:   YP_047859.1
- ProteinModelPortal:   Q6F7C8
- SMR:   Q6F7C8
- STRING:   Q6F7C8
- GeneID:   2880604
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD3371
- NMPDR:   fig|62977.3.peg.3052
- eggNOG:   COG0008
- HOGENOM:   HBG628189
- OMA:   MAHIPLI
- PhylomeDB:   Q6F7C8
- ProtClustDB:   PRK01406
- BioCyc:   ASP62977:ACIAD3371-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 57623; Mature: 57623

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 253-253

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVRTRIAPSPTGFPHVGTAYIALFNLCFAKQHGGEFILRIEDTDQLRSTPESEQMILDS
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHCCCCHHHHHHHH
LRWLGLNWSEGPDVGGPHAPYRQSERMGIYKQYALDLIEKGHAFYCFATAEELDQMRAEQ
HHHCCCCCCCCCCCCCCCCCCCCHHHCHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHH
QARGETPKYDGRGLKLSQEEVERRLAAGEPHVIRMKVPEEGICKFNDLLRGEVEIPWAQV
HHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHCCCCCCCHHHH
DMQVLLKTDGLPTYHLANVVDDHLMEITHVLRGEEWLPSAPKHQLLYQYFGWDMPTLCHM
HHHHEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCHHHCC
PLLRNPDKSKLSKRKNPTSINYYRDIGVLPEALLNYLGRMGWSMPDEREVFTLTDMIEHF
HHHCCCCHHHHHHCCCCCCCCHHHHHCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHC
DIQRVSLGGPIFDVDKLNWLNGQWIKSLTPGQLLDRLLSWKSDRQTLEDIATAIQPRINL
CCEEEECCCCEEECCCCCCCCCHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
LSEAVNWAGFYFNHMPNITAEMFESKKLSPEQVRQSLQFAIWRLESQFTWNNETVSQILM
HHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
DLANQMDIKLRDFMPAFFIAIAGSTSSTPVMQSMVTIGPDLTFARLRRALEIVGAPSKKE
HHHHHCCCHHHHHHHHHHHEECCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHH
LKVWEKLNESLKLPKNETVDNT
HHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MKVRTRIAPSPTGFPHVGTAYIALFNLCFAKQHGGEFILRIEDTDQLRSTPESEQMILDS
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHCCCCHHHHHHHH
LRWLGLNWSEGPDVGGPHAPYRQSERMGIYKQYALDLIEKGHAFYCFATAEELDQMRAEQ
HHHCCCCCCCCCCCCCCCCCCCCHHHCHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHH
QARGETPKYDGRGLKLSQEEVERRLAAGEPHVIRMKVPEEGICKFNDLLRGEVEIPWAQV
HHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHCCCCCCCHHHH
DMQVLLKTDGLPTYHLANVVDDHLMEITHVLRGEEWLPSAPKHQLLYQYFGWDMPTLCHM
HHHHEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCHHHCC
PLLRNPDKSKLSKRKNPTSINYYRDIGVLPEALLNYLGRMGWSMPDEREVFTLTDMIEHF
HHHCCCCHHHHHHCCCCCCCCHHHHHCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHC
DIQRVSLGGPIFDVDKLNWLNGQWIKSLTPGQLLDRLLSWKSDRQTLEDIATAIQPRINL
CCEEEECCCCEEECCCCCCCCCHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
LSEAVNWAGFYFNHMPNITAEMFESKKLSPEQVRQSLQFAIWRLESQFTWNNETVSQILM
HHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
DLANQMDIKLRDFMPAFFIAIAGSTSSTPVMQSMVTIGPDLTFARLRRALEIVGAPSKKE
HHHHHCCCHHHHHHHHHHHEECCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHH
LKVWEKLNESLKLPKNETVDNT
HHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA