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The map label for this gene is truB

Identifier: 50086292

GI number: 50086292

Start: 3213800

End: 3214756

Strand: Reverse

Name: truB

Synonym: ACIAD3307

Alternate gene names: 50086292

Gene position: 3214756-3213800 (Counterclockwise)

Preceding gene: 50086295

Following gene: 50086291

Centisome position: 89.33

GC content: 42.42

Gene sequence:

>957_bases
ATGAATGAATTTTTTAACATTATATTTAATTTATTTGATGTAGAGCACATTATGACTGCAAAATCCAGCAAGATATCGAG
ACGCCCTGTGAGTGGTGTATTTCTTTTGAATAAACCTTTGGGACTCAGCTCAAATGGTGTACTTCAAAAAGTCCGCTGGT
TGTTTCGTGCGCAAAAGGCTGGTCATACAGGTGCACTTGATCCACTGGCGACTGGTTTATTGCCGATTTGTTTAGGGGAG
GCGACCAAGTTTTCACATTATTTGCTTGACTCGACCAAGCGTTATCAAACCGTGGTCAAACTGGGACAAGTGACGACTAC
AGGTGATGTTGAAGGGAAAGTTGTACAGTCGCGTTCAGTCCCACCACTCACCCGTGACATGCTCGAATTGGTATTGGATG
GGTTTCGCGGCGATATCCAGCAAGTTCCGCCAATGTATTCTGCTTTAAAGCGTGATGGCCGCCCATTATATGAATTGGCA
CGTCAAGGAATTGAGATTGAGCGTGAAGCACGCCCAGTCACAATTCATGCTCTCAAACTTGTCGATTTTGATACAGAAAG
CCTAACGTTAGATGTGACATGTTCTAAAGGCACTTATATTCGCGTATTGGGTGAAGATATTGGTGAGGCATTAGGCTGTG
GTGGGCATTTAACTGCCTTGCATCGTGTACAGACTGGACATTTTGAACTTGTACCTGAATATACCCTTGAATATTTAGAA
CAACTGAATGAAACAGAGCGGGATATGCTATTATTGGCGCCCTACGCACCAGTCGATTATTTACCACAGGTTCAAATTCC
AGAAGGTCGTCTTAAATATTTCTGCAATGGACAAGAAAGCAATATTGACCACGATGCAGTACCAGAAGTTCTGGTATTTG
AGGGTGAACGTTGTTTAGGACTTGCTGAAATCACAGATAAAAAAAGATTGGTGCCGAAACGTCTTTTAAATTTATAG

Upstream 100 bases:

>100_bases
ATTCATGTTTTTATATTCCAATACGACGCAATTTTTATTTCATCATTGAATTGAATGATTATAAAAATAGCATAACTTTG
GACAAATAATACAAAAACGG

Downstream 100 bases:

>100_bases
GCATGACATTAGATGGAAATTGAAGTTCTTCTTAGCTTAATCTTTTTTGCGTTTTGTGCAGGTGCAATTGATGCGGCAGT
AGGAGGCGGAGGATTAATTC

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MNEFFNIIFNLFDVEHIMTAKSSKISRRPVSGVFLLNKPLGLSSNGVLQKVRWLFRAQKAGHTGALDPLATGLLPICLGE
ATKFSHYLLDSTKRYQTVVKLGQVTTTGDVEGKVVQSRSVPPLTRDMLELVLDGFRGDIQQVPPMYSALKRDGRPLYELA
RQGIEIEREARPVTIHALKLVDFDTESLTLDVTCSKGTYIRVLGEDIGEALGCGGHLTALHRVQTGHFELVPEYTLEYLE
QLNETERDMLLLAPYAPVDYLPQVQIPEGRLKYFCNGQESNIDHDAVPEVLVFEGERCLGLAEITDKKRLVPKRLLNL

Sequences:

>Translated_318_residues
MNEFFNIIFNLFDVEHIMTAKSSKISRRPVSGVFLLNKPLGLSSNGVLQKVRWLFRAQKAGHTGALDPLATGLLPICLGE
ATKFSHYLLDSTKRYQTVVKLGQVTTTGDVEGKVVQSRSVPPLTRDMLELVLDGFRGDIQQVPPMYSALKRDGRPLYELA
RQGIEIEREARPVTIHALKLVDFDTESLTLDVTCSKGTYIRVLGEDIGEALGCGGHLTALHRVQTGHFELVPEYTLEYLE
QLNETERDMLLLAPYAPVDYLPQVQIPEGRLKYFCNGQESNIDHDAVPEVLVFEGERCLGLAEITDKKRLVPKRLLNL
>Mature_318_residues
MNEFFNIIFNLFDVEHIMTAKSSKISRRPVSGVFLLNKPLGLSSNGVLQKVRWLFRAQKAGHTGALDPLATGLLPICLGE
ATKFSHYLLDSTKRYQTVVKLGQVTTTGDVEGKVVQSRSVPPLTRDMLELVLDGFRGDIQQVPPMYSALKRDGRPLYELA
RQGIEIEREARPVTIHALKLVDFDTESLTLDVTCSKGTYIRVLGEDIGEALGCGGHLTALHRVQTGHFELVPEYTLEYLE
QLNETERDMLLLAPYAPVDYLPQVQIPEGRLKYFCNGQESNIDHDAVPEVLVFEGERCLGLAEITDKKRLVPKRLLNL

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI21040257, Length=235, Percent_Identity=36.1702127659575, Blast_Score=138, Evalue=7e-33,
Organism=Homo sapiens, GI4503337, Length=198, Percent_Identity=31.3131313131313, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI215599015, Length=198, Percent_Identity=31.3131313131313, Blast_Score=75, Evalue=8e-14,
Organism=Escherichia coli, GI2367200, Length=302, Percent_Identity=46.3576158940397, Blast_Score=265, Evalue=3e-72,
Organism=Caenorhabditis elegans, GI17553978, Length=198, Percent_Identity=27.7777777777778, Blast_Score=82, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6324037, Length=191, Percent_Identity=35.6020942408377, Blast_Score=107, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6323204, Length=198, Percent_Identity=31.3131313131313, Blast_Score=85, Evalue=1e-17,
Organism=Drosophila melanogaster, GI281364189, Length=199, Percent_Identity=30.6532663316583, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI281364187, Length=199, Percent_Identity=30.6532663316583, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI281364185, Length=199, Percent_Identity=30.6532663316583, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI281364183, Length=199, Percent_Identity=30.6532663316583, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI62471759, Length=199, Percent_Identity=30.6532663316583, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI17975520, Length=199, Percent_Identity=30.6532663316583, Blast_Score=83, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUB_ACIAD (Q6F7I5)

Other databases:

- EMBL:   CR543861
- RefSeq:   YP_047802.1
- ProteinModelPortal:   Q6F7I5
- SMR:   Q6F7I5
- STRING:   Q6F7I5
- GeneID:   2880742
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD3307
- NMPDR:   fig|62977.3.peg.2995
- eggNOG:   COG0130
- HOGENOM:   HBG397258
- OMA:   YPAHMSK
- PhylomeDB:   Q6F7I5
- ProtClustDB:   PRK05033
- BioCyc:   ASP62977:ACIAD3307-MONOMER
- HAMAP:   MF_01080
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR015947
- InterPro:   IPR014780
- InterPro:   IPR015240
- TIGRFAMs:   TIGR00431

Pfam domain/function: PF09157 TruB-C_2; PF01509 TruB_N; SSF55120 PsdUridine_synth_cat_dom; SSF88697 PUA-like

EC number: 4.2.1.70

Molecular weight: Translated: 35617; Mature: 35617

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS00070 ALDEHYDE_DEHYDR_CYS

Important sites: ACT_SITE 67-67

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEFFNIIFNLFDVEHIMTAKSSKISRRPVSGVFLLNKPLGLSSNGVLQKVRWLFRAQKA
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHC
GHTGALDPLATGLLPICLGEATKFSHYLLDSTKRYQTVVKLGQVTTTGDVEGKVVQSRSV
CCCCCCCHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHCCEEECCCCCCEEECCCCC
PPLTRDMLELVLDGFRGDIQQVPPMYSALKRDGRPLYELARQGIEIEREARPVTIHALKL
CHHHHHHHHHHHHHHCCCHHHCCHHHHHHHHCCCHHHHHHHCCCCEECCCCCEEEEEEEE
VDFDTESLTLDVTCSKGTYIRVLGEDIGEALGCGGHLTALHRVQTGHFELVPEYTLEYLE
EECCCCCEEEEEEECCCCEEEEECHHHHHHHCCCCHHHHHHHCCCCCEEECHHHHHHHHH
QLNETERDMLLLAPYAPVDYLPQVQIPEGRLKYFCNGQESNIDHDAVPEVLVFEGERCLG
HHCCCCCCEEEECCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHCCCEEEECCCCCEE
LAEITDKKRLVPKRLLNL
HHHHCCHHHHHHHHHHCC
>Mature Secondary Structure
MNEFFNIIFNLFDVEHIMTAKSSKISRRPVSGVFLLNKPLGLSSNGVLQKVRWLFRAQKA
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHC
GHTGALDPLATGLLPICLGEATKFSHYLLDSTKRYQTVVKLGQVTTTGDVEGKVVQSRSV
CCCCCCCHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHCCEEECCCCCCEEECCCCC
PPLTRDMLELVLDGFRGDIQQVPPMYSALKRDGRPLYELARQGIEIEREARPVTIHALKL
CHHHHHHHHHHHHHHCCCHHHCCHHHHHHHHCCCHHHHHHHCCCCEECCCCCEEEEEEEE
VDFDTESLTLDVTCSKGTYIRVLGEDIGEALGCGGHLTALHRVQTGHFELVPEYTLEYLE
EECCCCCEEEEEEECCCCEEEEECHHHHHHHCCCCHHHHHHHCCCCCEEECHHHHHHHHH
QLNETERDMLLLAPYAPVDYLPQVQIPEGRLKYFCNGQESNIDHDAVPEVLVFEGERCLG
HHCCCCCCEEEECCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHCCCEEEECCCCCEE
LAEITDKKRLVPKRLLNL
HHHHCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA