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The map label for this gene is hprA [H]

Identifier: 50086267

GI number: 50086267

Start: 3189088

End: 3190044

Strand: Reverse

Name: hprA [H]

Synonym: ACIAD3278

Alternate gene names: 50086267

Gene position: 3190044-3189088 (Counterclockwise)

Preceding gene: 50086269

Following gene: 50086263

Centisome position: 88.65

GC content: 45.14

Gene sequence:

>957_bases
TTGAATGCAGCATTTTTAGATTACGGTTCGCTAGACATGAATGATCTGGATTTTAGTCCGTTGCAAGCATGTTTTAATGA
TCTACAGCTTTATTCTGCGAGTACTCCAGATCAGGTTATCCCTCGTTTAAAGGATGTTCAGGTCGCAATTACCAATAAAG
TCGTTATTAATGCCGAAGCATTAACACGGCTTCCCAAGCTCAAACTGATTTTGGTCTCTGCAACAGGTACCAATAATGTT
GATTTGAGGGCTGCCAAAGCACAGGGCATTGTGGTATGTAACTGTCAGGGCTACGGGACAGCTTCGGTGGCTCAACATAC
GCTCACCTTAATGCTGGCTCTTGCGACCTCGCTGCTGCGTTACGACCATGCTGTAGCACAAGGGCGTTGGCAGCAGGCAT
CACAGTTTTGTTTTTTGGATTATCCAATTATTGAATTATCTGGAAAAACACTCGGGATTGTTGGATATGGCGAGCTTGGG
AAAGAGGTGGCGCGTTTAGCTCAAGCCTTTGGTATGAAAATACTCATTGCCAATTTGCCTCAGCGTCCAAAACATGAAGA
CCGCTTGGAGCTTGAAGCACTTCTGCCACAGGTAGATTTCCTGAGTTTACATTGCCCATTGACTGAACACACTCAGCATT
TAATTGATGCTCACGCTTTTGCACTCATGAAGCCAAGTGCATTTCTGATTAACTGCGCACGTGGTGGCATCGTGCATGAA
CAAGCTTTACTCGATGCTCTAAAGCAGGGGAGAATTGCAGGGGCCGCGACAGATGTTTTGAGTATCGAACCACCAAAAAA
CGGCAATCCTTTGCTTGATGAAAAATTACCCAATCTGATTATTACCCCACATTCGGCATGGGGAAGTGTTCAGTCTCGAC
AACGTATGGTTGAGCAACTGGCTGAAAATGCAAAAGCGTTTAGTATTGGTCAGACCATACGTCAGGTCAATGCCTAA

Upstream 100 bases:

>100_bases
TCAGTCGGATAGTCATGATCTATGTATGAACAAAGTTTGCCAGCAAATAAAGAGCAGACGTTATTTTCTTCATAAATTTA
CAAAAGAGTCGAAGATGAGT

Downstream 100 bases:

>100_bases
GAATTAAGGGTTGGCACTTTCAGGCAGTGGAGTCGTTGTCGCCGATGGTGTAACACTCGATGTTTGTGGAACCAAGTTGT
TGGTCGCCTGAGATGATTGA

Product: glycerate dehydrogenase

Products: NA

Alternate protein names: GDH; Glyoxylate reductase; Hydroxypyruvate dehydrogenase; NADH-dependent hydroxypyruvate reductase; HPR; HPR-A [H]

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MNAAFLDYGSLDMNDLDFSPLQACFNDLQLYSASTPDQVIPRLKDVQVAITNKVVINAEALTRLPKLKLILVSATGTNNV
DLRAAKAQGIVVCNCQGYGTASVAQHTLTLMLALATSLLRYDHAVAQGRWQQASQFCFLDYPIIELSGKTLGIVGYGELG
KEVARLAQAFGMKILIANLPQRPKHEDRLELEALLPQVDFLSLHCPLTEHTQHLIDAHAFALMKPSAFLINCARGGIVHE
QALLDALKQGRIAGAATDVLSIEPPKNGNPLLDEKLPNLIITPHSAWGSVQSRQRMVEQLAENAKAFSIGQTIRQVNA

Sequences:

>Translated_318_residues
MNAAFLDYGSLDMNDLDFSPLQACFNDLQLYSASTPDQVIPRLKDVQVAITNKVVINAEALTRLPKLKLILVSATGTNNV
DLRAAKAQGIVVCNCQGYGTASVAQHTLTLMLALATSLLRYDHAVAQGRWQQASQFCFLDYPIIELSGKTLGIVGYGELG
KEVARLAQAFGMKILIANLPQRPKHEDRLELEALLPQVDFLSLHCPLTEHTQHLIDAHAFALMKPSAFLINCARGGIVHE
QALLDALKQGRIAGAATDVLSIEPPKNGNPLLDEKLPNLIITPHSAWGSVQSRQRMVEQLAENAKAFSIGQTIRQVNA
>Mature_318_residues
MNAAFLDYGSLDMNDLDFSPLQACFNDLQLYSASTPDQVIPRLKDVQVAITNKVVINAEALTRLPKLKLILVSATGTNNV
DLRAAKAQGIVVCNCQGYGTASVAQHTLTLMLALATSLLRYDHAVAQGRWQQASQFCFLDYPIIELSGKTLGIVGYGELG
KEVARLAQAFGMKILIANLPQRPKHEDRLELEALLPQVDFLSLHCPLTEHTQHLIDAHAFALMKPSAFLINCARGGIVHE
QALLDALKQGRIAGAATDVLSIEPPKNGNPLLDEKLPNLIITPHSAWGSVQSRQRMVEQLAENAKAFSIGQTIRQVNA

Specific function: Plays a central role in assimilation of carbon. It converts hydroxypyruvate to glycerate as a key step in the serine cycle, and may also play an important role in C2 reactions, by interconverting glyoxylate and glycolate [H]

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308577, Length=273, Percent_Identity=34.0659340659341, Blast_Score=151, Evalue=6e-37,
Organism=Homo sapiens, GI145580578, Length=278, Percent_Identity=35.9712230215827, Blast_Score=149, Evalue=4e-36,
Organism=Homo sapiens, GI4557499, Length=278, Percent_Identity=35.9712230215827, Blast_Score=149, Evalue=4e-36,
Organism=Homo sapiens, GI61743967, Length=272, Percent_Identity=36.0294117647059, Blast_Score=147, Evalue=1e-35,
Organism=Homo sapiens, GI4557497, Length=272, Percent_Identity=36.0294117647059, Blast_Score=147, Evalue=1e-35,
Organism=Homo sapiens, GI145580575, Length=268, Percent_Identity=36.9402985074627, Blast_Score=146, Evalue=2e-35,
Organism=Homo sapiens, GI6912396, Length=237, Percent_Identity=30.8016877637131, Blast_Score=102, Evalue=4e-22,
Organism=Escherichia coli, GI87082289, Length=254, Percent_Identity=33.0708661417323, Blast_Score=124, Evalue=9e-30,
Organism=Escherichia coli, GI1789279, Length=303, Percent_Identity=28.7128712871287, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1787645, Length=261, Percent_Identity=28.735632183908, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1788660, Length=282, Percent_Identity=27.3049645390071, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI87081824, Length=172, Percent_Identity=31.3953488372093, Blast_Score=63, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17532191, Length=258, Percent_Identity=34.1085271317829, Blast_Score=144, Evalue=4e-35,
Organism=Caenorhabditis elegans, GI25147481, Length=251, Percent_Identity=31.4741035856574, Blast_Score=112, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6320925, Length=306, Percent_Identity=31.6993464052288, Blast_Score=149, Evalue=6e-37,
Organism=Saccharomyces cerevisiae, GI6322116, Length=334, Percent_Identity=31.1377245508982, Blast_Score=149, Evalue=7e-37,
Organism=Saccharomyces cerevisiae, GI6324055, Length=319, Percent_Identity=27.8996865203762, Blast_Score=112, Evalue=6e-26,
Organism=Saccharomyces cerevisiae, GI6324964, Length=259, Percent_Identity=27.4131274131274, Blast_Score=94, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24646446, Length=266, Percent_Identity=36.8421052631579, Blast_Score=155, Evalue=3e-38,
Organism=Drosophila melanogaster, GI24646448, Length=266, Percent_Identity=36.8421052631579, Blast_Score=155, Evalue=3e-38,
Organism=Drosophila melanogaster, GI24646452, Length=266, Percent_Identity=36.8421052631579, Blast_Score=155, Evalue=3e-38,
Organism=Drosophila melanogaster, GI24646450, Length=266, Percent_Identity=36.8421052631579, Blast_Score=155, Evalue=3e-38,
Organism=Drosophila melanogaster, GI62472511, Length=266, Percent_Identity=36.8421052631579, Blast_Score=154, Evalue=8e-38,
Organism=Drosophila melanogaster, GI19921140, Length=242, Percent_Identity=34.297520661157, Blast_Score=141, Evalue=7e-34,
Organism=Drosophila melanogaster, GI28574286, Length=247, Percent_Identity=30.3643724696356, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI28571528, Length=264, Percent_Identity=31.4393939393939, Blast_Score=108, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24585514, Length=256, Percent_Identity=28.515625, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI28574282, Length=256, Percent_Identity=28.515625, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI28574284, Length=256, Percent_Identity=28.515625, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI45551003, Length=256, Percent_Identity=28.515625, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI45552429, Length=256, Percent_Identity=28.515625, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI24585516, Length=231, Percent_Identity=28.1385281385281, Blast_Score=97, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.29 [H]

Molecular weight: Translated: 34634; Mature: 34634

Theoretical pI: Translated: 6.99; Mature: 6.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAAFLDYGSLDMNDLDFSPLQACFNDLQLYSASTPDQVIPRLKDVQVAITNKVVINAEA
CCCEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHCCHHCEEEEEECEEEEEHHH
LTRLPKLKLILVSATGTNNVDLRAAKAQGIVVCNCQGYGTASVAQHTLTLMLALATSLLR
HHCCCCEEEEEEEECCCCCCEEEEECCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHH
YDHAVAQGRWQQASQFCFLDYPIIELSGKTLGIVGYGELGKEVARLAQAFGMKILIANLP
HHHHHHHCCHHHHCCEEEECCCEEEECCCEEEEEECHHHHHHHHHHHHHHCCEEEEECCC
QRPKHEDRLELEALLPQVDFLSLHCPLTEHTQHLIDAHAFALMKPSAFLINCARGGIVHE
CCCCCCHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHH
QALLDALKQGRIAGAATDVLSIEPPKNGNPLLDEKLPNLIITPHSAWGSVQSRQRMVEQL
HHHHHHHHHCCCCCCCCCEEEECCCCCCCCCHHHHCCCEEEECCCCCCCHHHHHHHHHHH
AENAKAFSIGQTIRQVNA
HHCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MNAAFLDYGSLDMNDLDFSPLQACFNDLQLYSASTPDQVIPRLKDVQVAITNKVVINAEA
CCCEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHCCHHCEEEEEECEEEEEHHH
LTRLPKLKLILVSATGTNNVDLRAAKAQGIVVCNCQGYGTASVAQHTLTLMLALATSLLR
HHCCCCEEEEEEEECCCCCCEEEEECCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHH
YDHAVAQGRWQQASQFCFLDYPIIELSGKTLGIVGYGELGKEVARLAQAFGMKILIANLP
HHHHHHHCCHHHHCCEEEECCCEEEECCCEEEEEECHHHHHHHHHHHHHHCCEEEEECCC
QRPKHEDRLELEALLPQVDFLSLHCPLTEHTQHLIDAHAFALMKPSAFLINCARGGIVHE
CCCCCCHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHH
QALLDALKQGRIAGAATDVLSIEPPKNGNPLLDEKLPNLIITPHSAWGSVQSRQRMVEQL
HHHHHHHHHCCCCCCCCCEEEECCCCCCCCCHHHHCCCEEEECCCCCCCHHHHHHHHHHH
AENAKAFSIGQTIRQVNA
HHCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8144463; 1729225; 1657886 [H]