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The map label for this gene is glyS

Identifier: 50086259

GI number: 50086259

Start: 3182243

End: 3184312

Strand: Direct

Name: glyS

Synonym: ACIAD3269

Alternate gene names: 50086259

Gene position: 3182243-3184312 (Clockwise)

Preceding gene: 50086258

Following gene: 50086260

Centisome position: 88.43

GC content: 43.04

Gene sequence:

>2070_bases
ATGAGCAAACATACTGTTTTATTCGAACTTGGCTGTGAAGAACTTCCACCAAAAAGCCTGAAAACTTTACGTGATGCATT
ACAGGCAGAAACGGTAAAAGGCTTAAAAGATGCAGGTTTAGCATTTGCATCAATTGAAGCTTATGCAGCGCCACGTCGTT
TAGCGCTTAAAATTGTCGATGTAGACGGCGCACAAGCGGACACACAGAAGCGTTTTGATGGCCCTGCTGTTCAAGCTGCG
TATGATGCTGAAGGCAAACCAACCAAAGCTCTTGAAGGCTTTATGCGTGGTCAAGGTATTACCGCTGATCAAGTTTCAAC
ATTCCAAGCAGGTAAAGTTGAAAAAGTTTGCTATTTAAAAGATGTGAAAGGTCAAACTCTTGATGCTTTACTTCCACAGA
TTTTACAGACTGCATTAGACAATTTACCAATTGCTAAGCGTATGCGCTCTGCGGCAAGCCGTACCGAATTTGTACGTCCT
GTAAAATGGGTAGTATTACTTAAAGATGATCAAATCATTGAAGCAACCATTCAAGACCATCAAGCAGGTAACGTGACGTA
TGGTCACCGTTTCCATGCACCTGAAGCTGTAACTTTAGCGCATGCAAATGACTATTTAGCGGCTTTAGAAAAAGCTTACG
TTGTTGCGAACTTTGAAAAACGTCAAGCAACCATTCAAGAACAAGTGAAAAAGTTAGCTGATGAAGTGAACGCGACTGCG
ATTGTTCCAGCTGACTTACTTGATGAAGTAACAAGCCTCGTTGAATGGCCAGTTGCTCTTCGTGCAAACTTTGAAGAGCG
CTTCCTTGCTGTTCCTCAAGAAGCACTCATTACAACGATGCAAGACAACCAGAAGTATTTCTGCTTGATCAATAGCGAAG
GTAAATTACAGCCTTACTTCATTACTGTTTCAAATATTGAGTCTAAAGACCCGACTCAAATTATTGAAGGTAATGAAAAA
GTTGTTCGCCCTCGTTTATCTGATGCTGAATTCTTCTTCTTACAAGATCAAAAGCAACCGCTTGCATCTCGTAAAGAAAA
ATTAGCAAACATGGTATTCCAAGCACAGCTTGGTACGCTTTGGGATAAATCAACACGTATTGCTAAACTTGCTGTTGCGT
TGTCTCCGATCACTGGTGCAAACCCAGCAGACGCTGAAAAAGCAGCCTTACTTGCTAAATGCGATTTAACTTCTGAGCTT
GTTGGTGAGTTCCCAGAACTTCAAGGGATTGCGGGTACTTACTACGCACGTATTGAAGGCGAAAACACTGAAGTTTCAGA
AGCTTTAGGTGAGCAATATTTACCAAAATTTGCGGGTGATGTTTTACCAAAAACCAAAACAGGTACAACGATTGCCCTTG
CTGACCGTTTAGATACGCTTGTTGGTATTTTCGGTATTGGACAAGCACCGACAGGTTCTAAAGACCCATTTGCCCTTCGC
CGTTCAGCAATTGGTATCTTGCGTTTAATCATTGAAAATGAGCTAGACGTAACCATTGAAGAATTGGTTAATCTTGCACT
TCAAGGCTATGGCGATATCGTGAAAGACCACGACAAGACTCTTGCTGATGCGGTTGCATTCTTGGAAGGTCGTTACCGTG
CCAAGTACGAAGACCAAGGCGTTGCGGTTGACGTACTTCAAGCGGTTCAAGCACTTGCACCGAAGTCTCCACTTGATTTT
GACAAGCGTGTAAATGCTGTTAACCACTTCCGTGCGCTACCTGAAGCTGCCGCACTTGCTGCTGCGAACAAACGTGTTGC
GAATATTCTTGCAAAAGAAGCGGCACCAGAAGGTTCTGTGGTTGAAGCAAACTTGGTTGAAGATGCTGAAAAAGCATTAT
TCGCTGAACTTCAAGCAGTTACCCCTGCTGTTGAACCATTACTTGCAGCAAAAGATTATACTGCTGCACTTTCTAAGCTT
GCAGCACTTCGCGCGCCGATTGATGCATTCTTTGATGGTGTAATGGTTATGGCAGATGATGCTGACCTAAAAGCAAACCG
TTTACGTTTATTGGCTCAGTTGCGTCACTTGTTTACTGTTGTGGCTGATGTGAGTGTGTTGCAGGGTTAA

Upstream 100 bases:

>100_bases
AGCTTGGCTTCCCAATGGCAGAACCACATTTGCGTGATGAAGTATTGGCACAGTTAAAAGCACAAGCTGAAAGTGAAGCA
GCAAAGGCGGAGAAAAACTA

Downstream 100 bases:

>100_bases
GTATTTATAATACTTGATCTTTAAAATAAACCCCGCTATAAAATTAGTGGGGTTTTTATTTAAATTTTTATAAAGTTAAT
ATAATTATGAATTCAGTAGC

Product: glycyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS

Number of amino acids: Translated: 689; Mature: 688

Protein sequence:

>689_residues
MSKHTVLFELGCEELPPKSLKTLRDALQAETVKGLKDAGLAFASIEAYAAPRRLALKIVDVDGAQADTQKRFDGPAVQAA
YDAEGKPTKALEGFMRGQGITADQVSTFQAGKVEKVCYLKDVKGQTLDALLPQILQTALDNLPIAKRMRSAASRTEFVRP
VKWVVLLKDDQIIEATIQDHQAGNVTYGHRFHAPEAVTLAHANDYLAALEKAYVVANFEKRQATIQEQVKKLADEVNATA
IVPADLLDEVTSLVEWPVALRANFEERFLAVPQEALITTMQDNQKYFCLINSEGKLQPYFITVSNIESKDPTQIIEGNEK
VVRPRLSDAEFFFLQDQKQPLASRKEKLANMVFQAQLGTLWDKSTRIAKLAVALSPITGANPADAEKAALLAKCDLTSEL
VGEFPELQGIAGTYYARIEGENTEVSEALGEQYLPKFAGDVLPKTKTGTTIALADRLDTLVGIFGIGQAPTGSKDPFALR
RSAIGILRLIIENELDVTIEELVNLALQGYGDIVKDHDKTLADAVAFLEGRYRAKYEDQGVAVDVLQAVQALAPKSPLDF
DKRVNAVNHFRALPEAAALAAANKRVANILAKEAAPEGSVVEANLVEDAEKALFAELQAVTPAVEPLLAAKDYTAALSKL
AALRAPIDAFFDGVMVMADDADLKANRLRLLAQLRHLFTVVADVSVLQG

Sequences:

>Translated_689_residues
MSKHTVLFELGCEELPPKSLKTLRDALQAETVKGLKDAGLAFASIEAYAAPRRLALKIVDVDGAQADTQKRFDGPAVQAA
YDAEGKPTKALEGFMRGQGITADQVSTFQAGKVEKVCYLKDVKGQTLDALLPQILQTALDNLPIAKRMRSAASRTEFVRP
VKWVVLLKDDQIIEATIQDHQAGNVTYGHRFHAPEAVTLAHANDYLAALEKAYVVANFEKRQATIQEQVKKLADEVNATA
IVPADLLDEVTSLVEWPVALRANFEERFLAVPQEALITTMQDNQKYFCLINSEGKLQPYFITVSNIESKDPTQIIEGNEK
VVRPRLSDAEFFFLQDQKQPLASRKEKLANMVFQAQLGTLWDKSTRIAKLAVALSPITGANPADAEKAALLAKCDLTSEL
VGEFPELQGIAGTYYARIEGENTEVSEALGEQYLPKFAGDVLPKTKTGTTIALADRLDTLVGIFGIGQAPTGSKDPFALR
RSAIGILRLIIENELDVTIEELVNLALQGYGDIVKDHDKTLADAVAFLEGRYRAKYEDQGVAVDVLQAVQALAPKSPLDF
DKRVNAVNHFRALPEAAALAAANKRVANILAKEAAPEGSVVEANLVEDAEKALFAELQAVTPAVEPLLAAKDYTAALSKL
AALRAPIDAFFDGVMVMADDADLKANRLRLLAQLRHLFTVVADVSVLQG
>Mature_688_residues
SKHTVLFELGCEELPPKSLKTLRDALQAETVKGLKDAGLAFASIEAYAAPRRLALKIVDVDGAQADTQKRFDGPAVQAAY
DAEGKPTKALEGFMRGQGITADQVSTFQAGKVEKVCYLKDVKGQTLDALLPQILQTALDNLPIAKRMRSAASRTEFVRPV
KWVVLLKDDQIIEATIQDHQAGNVTYGHRFHAPEAVTLAHANDYLAALEKAYVVANFEKRQATIQEQVKKLADEVNATAI
VPADLLDEVTSLVEWPVALRANFEERFLAVPQEALITTMQDNQKYFCLINSEGKLQPYFITVSNIESKDPTQIIEGNEKV
VRPRLSDAEFFFLQDQKQPLASRKEKLANMVFQAQLGTLWDKSTRIAKLAVALSPITGANPADAEKAALLAKCDLTSELV
GEFPELQGIAGTYYARIEGENTEVSEALGEQYLPKFAGDVLPKTKTGTTIALADRLDTLVGIFGIGQAPTGSKDPFALRR
SAIGILRLIIENELDVTIEELVNLALQGYGDIVKDHDKTLADAVAFLEGRYRAKYEDQGVAVDVLQAVQALAPKSPLDFD
KRVNAVNHFRALPEAAALAAANKRVANILAKEAAPEGSVVEANLVEDAEKALFAELQAVTPAVEPLLAAKDYTAALSKLA
ALRAPIDAFFDGVMVMADDADLKANRLRLLAQLRHLFTVVADVSVLQG

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Escherichia coli, GI1789982, Length=690, Percent_Identity=48.8405797101449, Blast_Score=619, Evalue=1e-178,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYGB_ACIAD (Q6F7L8)

Other databases:

- EMBL:   CR543861
- RefSeq:   YP_047769.1
- ProteinModelPortal:   Q6F7L8
- STRING:   Q6F7L8
- GeneID:   2880608
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD3269
- NMPDR:   fig|62977.3.peg.2962
- eggNOG:   COG0751
- HOGENOM:   HBG586599
- OMA:   FIRPVHT
- PhylomeDB:   Q6F7L8
- ProtClustDB:   PRK01233
- BioCyc:   ASP62977:ACIAD3269-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00255
- InterPro:   IPR008909
- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311
- PRINTS:   PR01045
- TIGRFAMs:   TIGR00211

Pfam domain/function: PF05746 DALR_1; PF02092 tRNA_synt_2f

EC number: =6.1.1.14

Molecular weight: Translated: 74936; Mature: 74805

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKHTVLFELGCEELPPKSLKTLRDALQAETVKGLKDAGLAFASIEAYAAPRRLALKIVD
CCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCEEEEEEEE
VDGAQADTQKRFDGPAVQAAYDAEGKPTKALEGFMRGQGITADQVSTFQAGKVEKVCYLK
CCCCCCCHHHHCCCCCEEEEECCCCCCHHHHHHHHCCCCCCHHHHHHCCCCCCCCEEEEE
DVKGQTLDALLPQILQTALDNLPIAKRMRSAASRTEFVRPVKWVVLLKDDQIIEATIQDH
CCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCEEEHHHHCC
QAGNVTYGHRFHAPEAVTLAHANDYLAALEKAYVVANFEKRQATIQEQVKKLADEVNATA
CCCCCCCCCEECCCCEEEEECCHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHHHCCCEE
IVPADLLDEVTSLVEWPVALRANFEERFLAVPQEALITTMQDNQKYFCLINSEGKLQPYF
EECHHHHHHHHHHHHCCHHHHCCHHHHHHHCCHHHHHHHEECCCEEEEEECCCCCEEEEE
ITVSNIESKDPTQIIEGNEKVVRPRLSDAEFFFLQDQKQPLASRKEKLANMVFQAQLGTL
EEEECCCCCCCHHHHCCCCCEECCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHH
WDKSTRIAKLAVALSPITGANPADAEKAALLAKCDLTSELVGEFPELQGIAGTYYARIEG
HCCCHHHHHHHHEECCCCCCCCCCHHHHHHHHHCCCCHHHHCCCHHHCCCCCEEEEEEEC
ENTEVSEALGEQYLPKFAGDVLPKTKTGTTIALADRLDTLVGIFGIGQAPTGSKDPFALR
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHCCCCCCCCCCCCHHHH
RSAIGILRLIIENELDVTIEELVNLALQGYGDIVKDHDKTLADAVAFLEGRYRAKYEDQG
HHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
VAVDVLQAVQALAPKSPLDFDKRVNAVNHFRALPEAAALAAANKRVANILAKEAAPEGSV
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCE
VEANLVEDAEKALFAELQAVTPAVEPLLAAKDYTAALSKLAALRAPIDAFFDGVMVMADD
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCEEEEECC
ADLKANRLRLLAQLRHLFTVVADVSVLQG
CCCHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SKHTVLFELGCEELPPKSLKTLRDALQAETVKGLKDAGLAFASIEAYAAPRRLALKIVD
CCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCEEEEEEEE
VDGAQADTQKRFDGPAVQAAYDAEGKPTKALEGFMRGQGITADQVSTFQAGKVEKVCYLK
CCCCCCCHHHHCCCCCEEEEECCCCCCHHHHHHHHCCCCCCHHHHHHCCCCCCCCEEEEE
DVKGQTLDALLPQILQTALDNLPIAKRMRSAASRTEFVRPVKWVVLLKDDQIIEATIQDH
CCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCEEEHHHHCC
QAGNVTYGHRFHAPEAVTLAHANDYLAALEKAYVVANFEKRQATIQEQVKKLADEVNATA
CCCCCCCCCEECCCCEEEEECCHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHHHCCCEE
IVPADLLDEVTSLVEWPVALRANFEERFLAVPQEALITTMQDNQKYFCLINSEGKLQPYF
EECHHHHHHHHHHHHCCHHHHCCHHHHHHHCCHHHHHHHEECCCEEEEEECCCCCEEEEE
ITVSNIESKDPTQIIEGNEKVVRPRLSDAEFFFLQDQKQPLASRKEKLANMVFQAQLGTL
EEEECCCCCCCHHHHCCCCCEECCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHH
WDKSTRIAKLAVALSPITGANPADAEKAALLAKCDLTSELVGEFPELQGIAGTYYARIEG
HCCCHHHHHHHHEECCCCCCCCCCHHHHHHHHHCCCCHHHHCCCHHHCCCCCEEEEEEEC
ENTEVSEALGEQYLPKFAGDVLPKTKTGTTIALADRLDTLVGIFGIGQAPTGSKDPFALR
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHCCCCCCCCCCCCHHHH
RSAIGILRLIIENELDVTIEELVNLALQGYGDIVKDHDKTLADAVAFLEGRYRAKYEDQG
HHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
VAVDVLQAVQALAPKSPLDFDKRVNAVNHFRALPEAAALAAANKRVANILAKEAAPEGSV
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCE
VEANLVEDAEKALFAELQAVTPAVEPLLAAKDYTAALSKLAALRAPIDAFFDGVMVMADD
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCEEEEECC
ADLKANRLRLLAQLRHLFTVVADVSVLQG
CCCHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA