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The map label for this gene is murI [H]

Identifier: 50086249

GI number: 50086249

Start: 3169717

End: 3170595

Strand: Reverse

Name: murI [H]

Synonym: ACIAD3257

Alternate gene names: 50086249

Gene position: 3170595-3169717 (Counterclockwise)

Preceding gene: 50086250

Following gene: 50086248

Centisome position: 88.11

GC content: 44.48

Gene sequence:

>879_bases
ATGACTGCAATATTTCCCGTTTCCCTAGAGCTTGAACCAATGCCAAAAGCCACAGCCGATGCGCCTATTGGTATTTTTGA
CTCGGGAATTGGTGGGCTGTCGATTGCTCAGGAAATTGCAAATTATTTGCCGAATGAACAGATTGTCTACTATGCAGATA
CTGCATTTGTTCCTTATGGGCCTCGTCCAGATCATGAAATTCGTCAGTTGACGGCACGTGGTATTGAGTGGCTTTATCGT
CAAGGCTGTAAAATGGCGGTTGTGGCCTGCAATACGGCTTCTGCTTTTAGTTTAGATGGTTTACGCGAGCATTATGGCGA
ATCTTTCCCTATTGTCGGGCTGGTTCCTGCATTGAAACCTGCGGTATTGCAAAGCCAGTCCAAAGTCGTTGCTGTACTTG
CCACGCCTGCTACCTTTCGTGGTCAGTTGATCAAGGACGTCATTTATCATTTTGCCTTACCTTCAGGGGTTCAGGTTCTT
CCTGTCACTTGTCTTGAACTGGTACCTTTGATTGAAGCAGGGCAGCAAATGAGTCAACCGTGTCTGGATCTACTCCGACA
GATACTGACTCCTGTGATTGATCAAGGAGCTGATTATCTGGTTCTTGGCTGTACGCACTATCCTTTTCTCAAAGATGCCA
TTCATTATTTATTTGGTAATCGTTTAACATTGATTGACTCAGGCGCACCTGTGGCGAGGCAAACCGCACGTATCTTGATT
AAACATCAATTGTTATGTGAAAAAAAACGAACAGGTCATTTGAGAATAGCATGTTATGTCAGTGGGCAAGAGGTTGAGGC
ATCAGGTAAAGTGATCCAGCATCTCATGCTTCCTCATTTGTCATGGACGATTGAGAAAATTCAGGCTTCTATCAGTTAA

Upstream 100 bases:

>100_bases
AAAGCCATTTTAGTATTAAAAAGTGGTGATTTGCTCTTGCAATTAGACAGTTGAGGTTTAAGCTTTAAGCTCAGATAAAT
CTTGTACTTGAAGTGCTGAA

Downstream 100 bases:

>100_bases
ACGTATTCTTGAAATCGTTTGACTCAGGAAATAATCCTGTTAAAAAGAAGATGAAGCAAACTTATTATGGCGAAACATAA
CTTGATTGGTGGAAAAGGAA

Product: glutamate racemase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 292; Mature: 291

Protein sequence:

>292_residues
MTAIFPVSLELEPMPKATADAPIGIFDSGIGGLSIAQEIANYLPNEQIVYYADTAFVPYGPRPDHEIRQLTARGIEWLYR
QGCKMAVVACNTASAFSLDGLREHYGESFPIVGLVPALKPAVLQSQSKVVAVLATPATFRGQLIKDVIYHFALPSGVQVL
PVTCLELVPLIEAGQQMSQPCLDLLRQILTPVIDQGADYLVLGCTHYPFLKDAIHYLFGNRLTLIDSGAPVARQTARILI
KHQLLCEKKRTGHLRIACYVSGQEVEASGKVIQHLMLPHLSWTIEKIQASIS

Sequences:

>Translated_292_residues
MTAIFPVSLELEPMPKATADAPIGIFDSGIGGLSIAQEIANYLPNEQIVYYADTAFVPYGPRPDHEIRQLTARGIEWLYR
QGCKMAVVACNTASAFSLDGLREHYGESFPIVGLVPALKPAVLQSQSKVVAVLATPATFRGQLIKDVIYHFALPSGVQVL
PVTCLELVPLIEAGQQMSQPCLDLLRQILTPVIDQGADYLVLGCTHYPFLKDAIHYLFGNRLTLIDSGAPVARQTARILI
KHQLLCEKKRTGHLRIACYVSGQEVEASGKVIQHLMLPHLSWTIEKIQASIS
>Mature_291_residues
TAIFPVSLELEPMPKATADAPIGIFDSGIGGLSIAQEIANYLPNEQIVYYADTAFVPYGPRPDHEIRQLTARGIEWLYRQ
GCKMAVVACNTASAFSLDGLREHYGESFPIVGLVPALKPAVLQSQSKVVAVLATPATFRGQLIKDVIYHFALPSGVQVLP
VTCLELVPLIEAGQQMSQPCLDLLRQILTPVIDQGADYLVLGCTHYPFLKDAIHYLFGNRLTLIDSGAPVARQTARILIK
HQLLCEKKRTGHLRIACYVSGQEVEASGKVIQHLMLPHLSWTIEKIQASIS

Specific function: Provides the (R)-glutamate required for cell wall biosynthesis [H]

COG id: COG0796

COG function: function code M; Glutamate racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aspartate/glutamate racemases family [H]

Homologues:

Organism=Escherichia coli, GI87082355, Length=222, Percent_Identity=37.8378378378378, Blast_Score=127, Evalue=8e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015942
- InterPro:   IPR001920
- InterPro:   IPR018187
- InterPro:   IPR004391 [H]

Pfam domain/function: PF01177 Asp_Glu_race [H]

EC number: =5.1.1.3 [H]

Molecular weight: Translated: 31983; Mature: 31852

Theoretical pI: Translated: 6.99; Mature: 6.99

Prosite motif: PS00923 ASP_GLU_RACEMASE_1 ; PS00924 ASP_GLU_RACEMASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAIFPVSLELEPMPKATADAPIGIFDSGIGGLSIAQEIANYLPNEQIVYYADTAFVPYG
CCEEEEEEEEECCCCCCCCCCCCCHHCCCCCHHHHHHHHHHHCCCCEEEEEECCEEECCC
PRPDHEIRQLTARGIEWLYRQGCKMAVVACNTASAFSLDGLREHYGESFPIVGLVPALKP
CCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHCCCCCCCEEEHHHHHH
AVLQSQSKVVAVLATPATFRGQLIKDVIYHFALPSGVQVLPVTCLELVPLIEAGQQMSQP
HHHCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHH
CLDLLRQILTPVIDQGADYLVLGCTHYPFLKDAIHYLFGNRLTLIDSGAPVARQTARILI
HHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHH
KHQLLCEKKRTGHLRIACYVSGQEVEASGKVIQHLMLPHLSWTIEKIQASIS
HHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TAIFPVSLELEPMPKATADAPIGIFDSGIGGLSIAQEIANYLPNEQIVYYADTAFVPYG
CEEEEEEEEECCCCCCCCCCCCCHHCCCCCHHHHHHHHHHHCCCCEEEEEECCEEECCC
PRPDHEIRQLTARGIEWLYRQGCKMAVVACNTASAFSLDGLREHYGESFPIVGLVPALKP
CCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHCCCCCCCEEEHHHHHH
AVLQSQSKVVAVLATPATFRGQLIKDVIYHFALPSGVQVLPVTCLELVPLIEAGQQMSQP
HHHCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHH
CLDLLRQILTPVIDQGADYLVLGCTHYPFLKDAIHYLFGNRLTLIDSGAPVARQTARILI
HHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHH
KHQLLCEKKRTGHLRIACYVSGQEVEASGKVIQHLMLPHLSWTIEKIQASIS
HHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10567266 [H]