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The map label for this gene is ttg2B [H]

Identifier: 50086238

GI number: 50086238

Start: 3157293

End: 3158069

Strand: Reverse

Name: ttg2B [H]

Synonym: ACIAD3243

Alternate gene names: 50086238

Gene position: 3158069-3157293 (Counterclockwise)

Preceding gene: 50086239

Following gene: 50086237

Centisome position: 87.76

GC content: 45.56

Gene sequence:

>777_bases
ATGAATGCGATTGCCTGGTTAGGCCGGCTGGTGATTGAACGTGTTCGAGGGATCGGTGTTGCCGCCCTCATGTTGTTACA
GATTTTATTTTCGATGCCCTCTACGGGTGGGTTTCGGCGCTTTATTTATCAGATGTATCGTGTCGGTGTGATGTCCTTAT
TGATTATCACTGTTTCAGGCTTGTTTATTGGTCTGGTGATTGGCCTGCAAGGCTATTCCATTCTGGTCAATGTTGGCAGT
GAAGCGATGCTGGGTACAATGGTTTCACTTACGCTGCTGCGTGAGTTGGCACCTGTAGTTGCTGCTTTACTATTTGCTGG
ACGGGCAGGTTCTGCTTTAACTGCCGAAATTGGTTCCATGAAGCAAAGTGAACAACTGGCCAGTATGGAAATGATTGGTG
TTGATCCGCTTAAGCAGATCGTTTCACCGCGCTTATGGGCAGGTATTGTGAGCCTACCCATGCTCACCGTGATTTTTGCC
GCGATTGGAATTTTAGGCGGCAAAATGGTAGGTGTGGATTTTCTTGGCGTGGATGAAGGATCTTTCTGGAGCGGTATGCA
AAGTAATGTTCAGTTTGGACACGATGTGGTCAATGGCATTATTAAAAGTATTGTATTTGCCTTAGTGTGTACTTGGATTG
CAGTTTATCAAGGATATGCCTGTGATCCGACACCTGAGGGCATTGCAACTTCAATGACACGTACCGTTGTTTATTCTTCT
TTGTGTGTATTGGGATCAGATTTTGTTTTGACTGCGGTCATGTTCGGAGGTATTTAA

Upstream 100 bases:

>100_bases
ATGCTTCCCCATTTGTGAAACAATTTCTGACGGGTTCAGAGGAAGGACCTGTTGAATATCAGTTTAGTCAACGCGCTTAT
TTAGATAACGAGGTATATCC

Downstream 100 bases:

>100_bases
TGAAATCACGTACAAGTGAGCTGGCCGCAGGAATTTTTGTCATTATTTTCGGCGTCGCCTTGTTCTTTTTAGCAATGAAA
GTCAGTGGATTGGCAGGTAC

Product: toluene tolerance efflux ABC transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MNAIAWLGRLVIERVRGIGVAALMLLQILFSMPSTGGFRRFIYQMYRVGVMSLLIITVSGLFIGLVIGLQGYSILVNVGS
EAMLGTMVSLTLLRELAPVVAALLFAGRAGSALTAEIGSMKQSEQLASMEMIGVDPLKQIVSPRLWAGIVSLPMLTVIFA
AIGILGGKMVGVDFLGVDEGSFWSGMQSNVQFGHDVVNGIIKSIVFALVCTWIAVYQGYACDPTPEGIATSMTRTVVYSS
LCVLGSDFVLTAVMFGGI

Sequences:

>Translated_258_residues
MNAIAWLGRLVIERVRGIGVAALMLLQILFSMPSTGGFRRFIYQMYRVGVMSLLIITVSGLFIGLVIGLQGYSILVNVGS
EAMLGTMVSLTLLRELAPVVAALLFAGRAGSALTAEIGSMKQSEQLASMEMIGVDPLKQIVSPRLWAGIVSLPMLTVIFA
AIGILGGKMVGVDFLGVDEGSFWSGMQSNVQFGHDVVNGIIKSIVFALVCTWIAVYQGYACDPTPEGIATSMTRTVVYSS
LCVLGSDFVLTAVMFGGI
>Mature_258_residues
MNAIAWLGRLVIERVRGIGVAALMLLQILFSMPSTGGFRRFIYQMYRVGVMSLLIITVSGLFIGLVIGLQGYSILVNVGS
EAMLGTMVSLTLLRELAPVVAALLFAGRAGSALTAEIGSMKQSEQLASMEMIGVDPLKQIVSPRLWAGIVSLPMLTVIFA
AIGILGGKMVGVDFLGVDEGSFWSGMQSNVQFGHDVVNGIIKSIVFALVCTWIAVYQGYACDPTPEGIATSMTRTVVYSS
LCVLGSDFVLTAVMFGGI

Specific function: Part of the ABC transporter complex mlaFEDB that actively prevents phospholipid accumulation at the cell surface. Probably maintains lipid asymmetry in the outer membrane by retrograde trafficking of phospholipids from the outer membrane to the inner memb

COG id: COG0767

COG function: function code Q; ABC-type transport system involved in resistance to organic solvents, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mlaE permease family [H]

Homologues:

Organism=Escherichia coli, GI1789585, Length=261, Percent_Identity=57.4712643678161, Blast_Score=292, Evalue=2e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003453 [H]

Pfam domain/function: PF02405 DUF140 [H]

EC number: NA

Molecular weight: Translated: 27398; Mature: 27398

Theoretical pI: Translated: 7.18; Mature: 7.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
5.8 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
5.8 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHCCHHHHHHHHHCCC
>Mature Secondary Structure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HHHHCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]