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The map label for this gene is ttg2C [H]

Identifier: 50086237

GI number: 50086237

Start: 3156622

End: 3157293

Strand: Reverse

Name: ttg2C [H]

Synonym: ACIAD3242

Alternate gene names: 50086237

Gene position: 3157293-3156622 (Counterclockwise)

Preceding gene: 50086238

Following gene: 50086236

Centisome position: 87.74

GC content: 40.03

Gene sequence:

>672_bases
ATGAAATCACGTACAAGTGAGCTGGCCGCAGGAATTTTTGTCATTATTTTCGGCGTCGCCTTGTTCTTTTTAGCAATGAA
AGTCAGTGGATTGGCAGGTACAAATCTCAGTGATCCTTATACCATGACTGCACAATTTGATAACGTGAATGGTTTAAAAC
CCCGTGCTAAAGTGACGATGAGTGGTGTTACGATTGGTCGAGTAGATGCAATTACACTCGATCCTGTCACACGTTTAGCT
ACAGTCACTTTTGACCTAGATGGCAAACTGACTACATTTAATGCAAAACAGTTAAAAACGGTAGAGCAAAATGCACTAGA
AGAACTACATTACAGTTCTGATTATAAGTCTGCTGAACCAGCGAAACAAAAAGTCATGGAACAGCAACTGATCGATAACA
TGAAATCCATTACCAGTATCGATGAAGATGCTTACATTATGGTATCGACTAACGGTTTATTGGGAGAAAAGTACCTGAAG
ATTGTTCCTGGCGGTGGCTTGAACTATCTCAAACGTGGTGATGCGATTACCAATACACAAGGTACGATGGATCTCGAAGA
TTTAATTAGTAAATTTATTACTGGTGGTGCAGGTAAATCATCAGACAACTCAACTGGAACAGCGGCTCAGTCTTCATCAT
CTGAAGATAAAGAACCTTCTTTCACTGAGTAA

Upstream 100 bases:

>100_bases
AGGGCATTGCAACTTCAATGACACGTACCGTTGTTTATTCTTCTTTGTGTGTATTGGGATCAGATTTTGTTTTGACTGCG
GTCATGTTCGGAGGTATTTA

Downstream 100 bases:

>100_bases
ATTTTAGGAGTTATGTCAATGAAAACATTATTAAAGCAAACGCTTACAGTAAGTGTATTATCTAGCATGCTGATAGGTAC
TGCTTTTGCTGCACCCACTG

Product: toluene tolerance efflux ABC transporter periplasmic substrate-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 223; Mature: 223

Protein sequence:

>223_residues
MKSRTSELAAGIFVIIFGVALFFLAMKVSGLAGTNLSDPYTMTAQFDNVNGLKPRAKVTMSGVTIGRVDAITLDPVTRLA
TVTFDLDGKLTTFNAKQLKTVEQNALEELHYSSDYKSAEPAKQKVMEQQLIDNMKSITSIDEDAYIMVSTNGLLGEKYLK
IVPGGGLNYLKRGDAITNTQGTMDLEDLISKFITGGAGKSSDNSTGTAAQSSSSEDKEPSFTE

Sequences:

>Translated_223_residues
MKSRTSELAAGIFVIIFGVALFFLAMKVSGLAGTNLSDPYTMTAQFDNVNGLKPRAKVTMSGVTIGRVDAITLDPVTRLA
TVTFDLDGKLTTFNAKQLKTVEQNALEELHYSSDYKSAEPAKQKVMEQQLIDNMKSITSIDEDAYIMVSTNGLLGEKYLK
IVPGGGLNYLKRGDAITNTQGTMDLEDLISKFITGGAGKSSDNSTGTAAQSSSSEDKEPSFTE
>Mature_223_residues
MKSRTSELAAGIFVIIFGVALFFLAMKVSGLAGTNLSDPYTMTAQFDNVNGLKPRAKVTMSGVTIGRVDAITLDPVTRLA
TVTFDLDGKLTTFNAKQLKTVEQNALEELHYSSDYKSAEPAKQKVMEQQLIDNMKSITSIDEDAYIMVSTNGLLGEKYLK
IVPGGGLNYLKRGDAITNTQGTMDLEDLISKFITGGAGKSSDNSTGTAAQSSSSEDKEPSFTE

Specific function: Part of the ABC transporter complex mlaFEDB that actively prevents phospholipid accumulation at the cell surface. Probably maintains lipid asymmetry in the outer membrane by retrograde trafficking of phospholipids from the outer membrane to the inner memb

COG id: COG1463

COG function: function code Q; ABC-type transport system involved in resistance to organic solvents, periplasmic component

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein; Periplasmic side (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mlaD family [H]

Homologues:

Organism=Escherichia coli, GI1789584, Length=225, Percent_Identity=30.2222222222222, Blast_Score=90, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003399 [H]

Pfam domain/function: PF02470 MCE [H]

EC number: NA

Molecular weight: Translated: 23958; Mature: 23958

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSRTSELAAGIFVIIFGVALFFLAMKVSGLAGTNLSDPYTMTAQFDNVNGLKPRAKVTM
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCCCCCEEEE
SGVTIGRVDAITLDPVTRLATVTFDLDGKLTTFNAKQLKTVEQNALEELHYSSDYKSAEP
CCEEECCEEEEEECCHHEEEEEEEECCCCEEECCHHHHHHHHHHHHHHHHCCCCCCCCCH
AKQKVMEQQLIDNMKSITSIDEDAYIMVSTNGLLGEKYLKIVPGGGLNYLKRGDAITNTQ
HHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCEEEEECCCCCHHHHCCCCCCCCC
GTMDLEDLISKFITGGAGKSSDNSTGTAAQSSSSEDKEPSFTE
CCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKSRTSELAAGIFVIIFGVALFFLAMKVSGLAGTNLSDPYTMTAQFDNVNGLKPRAKVTM
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCCCCCEEEE
SGVTIGRVDAITLDPVTRLATVTFDLDGKLTTFNAKQLKTVEQNALEELHYSSDYKSAEP
CCEEECCEEEEEECCHHEEEEEEEECCCCEEECCHHHHHHHHHHHHHHHHCCCCCCCCCH
AKQKVMEQQLIDNMKSITSIDEDAYIMVSTNGLLGEKYLKIVPGGGLNYLKRGDAITNTQ
HHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCEEEEECCCCCHHHHCCCCCCCCC
GTMDLEDLISKFITGGAGKSSDNSTGTAAQSSSSEDKEPSFTE
CCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]