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The map label for this gene is mdh

Identifier: 50086156

GI number: 50086156

Start: 3081535

End: 3082521

Strand: Reverse

Name: mdh

Synonym: ACIAD3155

Alternate gene names: 50086156

Gene position: 3082521-3081535 (Counterclockwise)

Preceding gene: 50086157

Following gene: 50086155

Centisome position: 85.66

GC content: 44.68

Gene sequence:

>987_bases
ATGAAGCAACCCGTTCGTGTTGCCGTTACTGGCGCTGCAGGTCAAATTGGTTATAGCTTGTTATTCCGTATTGCAAGCGG
AGAGATGTTAGGGAAAGATCAACCAGTTATTTTGCAATTGCTTGAAATTCCAGTTGAGAAAGCTCAACAAGCGCTTAAAG
GCGTAATGATGGAACTTGACGACTGTGCTTTCCCATTATTGGCTGGCATGATCGGTACAGATGATCCAAAAGTTGCATTT
AAAGATGCTGACTATGCATTACTTGTGGGTTCTCGTCCGCGTGGTCCTGGCATGGAACGCGCTGACTTATTGAAAGTAAA
TGGTGAAATCTTCATTGGTCAAGGTCAAGCCCTGAATGAAGTGGCAAGCCGTGACGTTAAAGTATTGGTTGTAGGTAACC
CTGCCAACACTAACGCTTACATCGCAATGAAATCTGCACCAGACCTTCCAGCGAAAAACTTCACTGCAATGTTGCGTCTA
GATCATAACCGTGCGTTGACTCAAATTGCTCAAAAAGCAGGTGTTGCTGTAGCGGACATCGAGAAGCTAACCGTTTGGGG
TAACCACTCTCCAACTATGTATGCTGACTATCGCTTTGCAACTGCAAATGGCGAAAACCTAAAAGACAAAATCAACGATG
CGGAATGGAACAAAGATGTGTTCCTTCCAACTGTTGGTAAACGTGGTGCTGCGATCATCGAAGCGCGTGGTTTGTCTTCT
GCTGCATCTGCTGCGAACGCTGCAATCGACCATATGCGTGACTGGGCACTTGGTACCAATGGTAAATGGGTCACTATGGG
TATTCCATCTGATGGTTCTTATGGTATTCCAGAAGGTGTTATTTACGGTGTACCTGTAACTTGCGAAAATGGCGAATACA
AACGTGTTGAAGGTCTTGAAATTGACGAGTTCAGCCGTGAACGTATGGATAAAACACTTCAGGAACTTGAAGAAGAACGT
GCTGCAATTGCTGACATGTTAAAATAA

Upstream 100 bases:

>100_bases
CTGTTTAAGGTTTGGAGTACAATTCTTGCCGTTTATGATTATTAGATTAAATGGACTATCTGTTTAATCATCTTAACGAT
CCTCAATTGGAGATAATTAC

Downstream 100 bases:

>100_bases
GATCATCTGATGAAGATGGAAAGGCACTTCAAATGAAGTGCCTTTTTTTATCCAATACAAGTTTAAAAAGCCTAAAGTAA
ACAAACCATAACAAGAATAT

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MKQPVRVAVTGAAGQIGYSLLFRIASGEMLGKDQPVILQLLEIPVEKAQQALKGVMMELDDCAFPLLAGMIGTDDPKVAF
KDADYALLVGSRPRGPGMERADLLKVNGEIFIGQGQALNEVASRDVKVLVVGNPANTNAYIAMKSAPDLPAKNFTAMLRL
DHNRALTQIAQKAGVAVADIEKLTVWGNHSPTMYADYRFATANGENLKDKINDAEWNKDVFLPTVGKRGAAIIEARGLSS
AASAANAAIDHMRDWALGTNGKWVTMGIPSDGSYGIPEGVIYGVPVTCENGEYKRVEGLEIDEFSRERMDKTLQELEEER
AAIADMLK

Sequences:

>Translated_328_residues
MKQPVRVAVTGAAGQIGYSLLFRIASGEMLGKDQPVILQLLEIPVEKAQQALKGVMMELDDCAFPLLAGMIGTDDPKVAF
KDADYALLVGSRPRGPGMERADLLKVNGEIFIGQGQALNEVASRDVKVLVVGNPANTNAYIAMKSAPDLPAKNFTAMLRL
DHNRALTQIAQKAGVAVADIEKLTVWGNHSPTMYADYRFATANGENLKDKINDAEWNKDVFLPTVGKRGAAIIEARGLSS
AASAANAAIDHMRDWALGTNGKWVTMGIPSDGSYGIPEGVIYGVPVTCENGEYKRVEGLEIDEFSRERMDKTLQELEEER
AAIADMLK
>Mature_328_residues
MKQPVRVAVTGAAGQIGYSLLFRIASGEMLGKDQPVILQLLEIPVEKAQQALKGVMMELDDCAFPLLAGMIGTDDPKVAF
KDADYALLVGSRPRGPGMERADLLKVNGEIFIGQGQALNEVASRDVKVLVVGNPANTNAYIAMKSAPDLPAKNFTAMLRL
DHNRALTQIAQKAGVAVADIEKLTVWGNHSPTMYADYRFATANGENLKDKINDAEWNKDVFLPTVGKRGAAIIEARGLSS
AASAANAAIDHMRDWALGTNGKWVTMGIPSDGSYGIPEGVIYGVPVTCENGEYKRVEGLEIDEFSRERMDKTLQELEEER
AAIADMLK

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 2 family

Homologues:

Organism=Homo sapiens, GI5174539, Length=333, Percent_Identity=52.5525525525526, Blast_Score=336, Evalue=2e-92,
Organism=Homo sapiens, GI89886456, Length=337, Percent_Identity=25.2225519287834, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17561064, Length=326, Percent_Identity=52.1472392638037, Blast_Score=320, Evalue=8e-88,
Organism=Caenorhabditis elegans, GI32566798, Length=264, Percent_Identity=51.8939393939394, Blast_Score=249, Evalue=2e-66,
Organism=Caenorhabditis elegans, GI32566800, Length=169, Percent_Identity=48.5207100591716, Blast_Score=154, Evalue=7e-38,
Organism=Drosophila melanogaster, GI24583394, Length=325, Percent_Identity=53.2307692307692, Blast_Score=320, Evalue=1e-87,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): MDH_ACIAD (Q6F7X1)

Other databases:

- EMBL:   CR543861
- RefSeq:   YP_047666.1
- ProteinModelPortal:   Q6F7X1
- SMR:   Q6F7X1
- STRING:   Q6F7X1
- GeneID:   2880577
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD3155
- NMPDR:   fig|62977.3.peg.2859
- eggNOG:   COG0039
- HOGENOM:   HBG289884
- OMA:   MIIWGNH
- ProtClustDB:   PRK05442
- BioCyc:   ASP62977:ACIAD3155-MONOMER
- GO:   GO:0005488
- GO:   GO:0006096
- HAMAP:   MF_01517
- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR010945
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.90.110.10
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR23382
- PIRSF:   PIRSF000102
- TIGRFAMs:   TIGR01759

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N; SSF56327 Lactate_DH/Glyco_hydro_4_C

EC number: =1.1.1.37

Molecular weight: Translated: 35449; Mature: 35449

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS00068 MDH

Important sites: ACT_SITE 189-189 BINDING 94-94 BINDING 100-100 BINDING 107-107 BINDING 114-114 BINDING 133-133 BINDING 164-164

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQPVRVAVTGAAGQIGYSLLFRIASGEMLGKDQPVILQLLEIPVEKAQQALKGVMMELD
CCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHH
DCAFPLLAGMIGTDDPKVAFKDADYALLVGSRPRGPGMERADLLKVNGEIFIGQGQALNE
HHHHHHHHHHCCCCCCCEEEECCCEEEEECCCCCCCCCCCCEEEEECCEEEEECCCHHHH
VASRDVKVLVVGNPANTNAYIAMKSAPDLPAKNFTAMLRLDHNRALTQIAQKAGVAVADI
HHCCCEEEEEECCCCCCCEEEEECCCCCCCCCCEEEEEEECCHHHHHHHHHHHCCEEEEE
EKLTVWGNHSPTMYADYRFATANGENLKDKINDAEWNKDVFLPTVGKRGAAIIEARGLSS
EEEEEECCCCCCEEEEEEEEECCCCCHHHHCCCCCCCCCEEEECCCCCCCEEEEECCCCH
AASAANAAIDHMRDWALGTNGKWVTMGIPSDGSYGIPEGVIYGVPVTCENGEYKRVEGLE
HHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCEEEEEEEEECCCCEEEECCCC
IDEFSRERMDKTLQELEEERAAIADMLK
HHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKQPVRVAVTGAAGQIGYSLLFRIASGEMLGKDQPVILQLLEIPVEKAQQALKGVMMELD
CCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHH
DCAFPLLAGMIGTDDPKVAFKDADYALLVGSRPRGPGMERADLLKVNGEIFIGQGQALNE
HHHHHHHHHHCCCCCCCEEEECCCEEEEECCCCCCCCCCCCEEEEECCEEEEECCCHHHH
VASRDVKVLVVGNPANTNAYIAMKSAPDLPAKNFTAMLRLDHNRALTQIAQKAGVAVADI
HHCCCEEEEEECCCCCCCEEEEECCCCCCCCCCEEEEEEECCHHHHHHHHHHHCCEEEEE
EKLTVWGNHSPTMYADYRFATANGENLKDKINDAEWNKDVFLPTVGKRGAAIIEARGLSS
EEEEEECCCCCCEEEEEEEEECCCCCHHHHCCCCCCCCCEEEECCCCCCCEEEEECCCCH
AASAANAAIDHMRDWALGTNGKWVTMGIPSDGSYGIPEGVIYGVPVTCENGEYKRVEGLE
HHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCEEEEEEEEECCCCEEEECCCC
IDEFSRERMDKTLQELEEERAAIADMLK
HHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA