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The map label for this gene is mnmC

Identifier: 50086138

GI number: 50086138

Start: 3060030

End: 3061901

Strand: Direct

Name: mnmC

Synonym: ACIAD3135

Alternate gene names: 50086138

Gene position: 3060030-3061901 (Clockwise)

Preceding gene: 50086137

Following gene: 50086140

Centisome position: 85.03

GC content: 43.06

Gene sequence:

>1872_bases
CTGTGCGTGTCTTCAAGCATTCAAACTGCAATTTTAGATTGGCAATGTGTCGATGGAATTGATATTCCAGTTTCTCGTCA
GTTTGGTGATGTCTATTTTTCCAAAGATAATGGTCTTCTCGAAACACGCCATGTATTTTTAAATGGCAATAATCTCACCG
AGCGACTGGCAGACTTAAAGCCGTATCAATATTTTTGTGTCGGAGAGACAGGTTTTGGTACAGGTCTTAATATTCTGGCA
GTATGGCAACTCTGGCAACAGGTACGACCCGATAATCATAGCCATCTGCATGTGATTAGCGTAGAAAAATTTCCTTTGTC
TAAAAGCGATCTGGAACGTGCGCTTCGTGCTTGGCCAGAGTTAATAATACTTGCAGAGCAACTGATTGAGCAGTATCCCT
TTCCAATTGCAGGCTGTCATCGCTTAAATTTTCCAGATGAGCGTTTTAGTCTGGATTTATGGTTAGGAGATGCACACGAT
GTATTTCCATCCATCGTCAAAACCCATTCTGTTGATGCATGGTTCCTAGATGGCTTTGCCCCTTCATGTAATCCTGAACT
CTGGGAAACGCAAGTACTGAACCAAATTGTGCGACTATCAGATTATGGTACGACGTTTGCCTCATTTAGTGTTGCAGGTA
TTTTAAAGCGTGGTCTGAAAGCACATGGTATTCACATTTCACGTCCTCGTGGGTTTGGTCATAAACGCGAAATGCTCAAG
GCAATATGGCCATTTCCCGACCATGAATCAACCACTACGGTAAAAGGCAATCAGCCATCTACACAGCAGCATTTTGCAGT
GATTGGCGCGGGGATCGCTGGTCTTCACTGTGCATGGTCTCTGGCACAGCGCGGCCATCGCGTGACTTTAGTTGATCAGT
CTCAACCTTTGTCAGGGGCTTCTGGTAATCCTTTAGCATTACTCAATCCAAAATTATGCCCGATCACTCAATGCCATGAT
CATCTGATGGTACTGTGCTGGCAGTATGCCCGTCGTTTTTATCAAAACTTCGAAGCCTTCCGCCTCATTCAGGTCAATCA
GCTTGCACTCAAAGCATCTGATTCATTGTTAAATCTGGCTGAGCAATATCCCAAAGATGTACTAGATGCACATAGCGCGC
AACAATCACCTCTTGAAACTCAATATGATTATTTGACACTACATTATGCAGGTGTTATTTCTCCGCAACTGTTTTGCAAT
CAAGTATTACAGCATCCTCTGATCGAATTTAAGCAGTTTAAAATCAATCAGATCATTGAAATCGATGGCACTGTTCAACT
GCTGTCTAGTGAGCAAGCCATTTTGGAGAGCAACGCTGCCATTGTTTGCTGTGCCAGAGAAAGTGGAAAGCTATTTGAAC
AGTATCCAACGCTTAAACCAATTCGTGGTCAGGTGAGTTGGTTTGAGCAACATACTTTTCCTTTTGCACCAGATCAGGCT
TTTAGTTATGGCGGTTACTGTATGCAGCTTCATCCGCAACAATTAATTCTGGGTGCATCCTTCCATCCACAGCGTGATGA
TGATGAAGTGTTGCTTGAGGATCATGTGCATAACTTTAATTTAATCCACAGTGTCTTCCCTGCGTATGCTCAAACATTGC
CACCTGTTGAAGAATGGCATGGACGTGCCTCAGTACGCGCACAGTCACCCGATTATTTTCCACTGGTTGGGCAGCTACAA
GACCATTCAAAGCTGTTTACGCTCGCAGGGCTTGGTTCTAAAGGCTATTTGTTTGCACCTTTAAATAGTGAAATTTTAGT
CGCGCATATTTTAGGGGAAGCTTGTCCTGTATCTGCCAATCTATTACAAAAACTGAATCCCCAGCGTTTCCTCAAAAAAG
TTAAAATCAAAAAACCGTATTATTCTTCATAG

Upstream 100 bases:

>100_bases
CAGTTGGTGTGGTTCTGGGCTTTTTTATTGCCAGTTATGTTTATACCAAACGCCGTCGCCAGTGGTAAACTCACTGGCAT
ATTCTTGTTGTTAAAGGTCT

Downstream 100 bases:

>100_bases
CCCAATGACTCTTTCCATTCAAAAAAAGCGCCCGAAGGCGCTTTTTGAGTTTAGCTATTTTGATCAAGTGGGATGCCAGC
AGCCTGCGCTGCGCGAGTAC

Product: hypothetical protein

Products: NA

Alternate protein names: tRNA mnm(5)s(2)U biosynthesis bifunctional protein; tRNA (mnm(5)s(2)U34)-methyltransferase; FAD-dependent cmnm(5)s(2)U34 oxidoreductase

Number of amino acids: Translated: 623; Mature: 623

Protein sequence:

>623_residues
MCVSSSIQTAILDWQCVDGIDIPVSRQFGDVYFSKDNGLLETRHVFLNGNNLTERLADLKPYQYFCVGETGFGTGLNILA
VWQLWQQVRPDNHSHLHVISVEKFPLSKSDLERALRAWPELIILAEQLIEQYPFPIAGCHRLNFPDERFSLDLWLGDAHD
VFPSIVKTHSVDAWFLDGFAPSCNPELWETQVLNQIVRLSDYGTTFASFSVAGILKRGLKAHGIHISRPRGFGHKREMLK
AIWPFPDHESTTTVKGNQPSTQQHFAVIGAGIAGLHCAWSLAQRGHRVTLVDQSQPLSGASGNPLALLNPKLCPITQCHD
HLMVLCWQYARRFYQNFEAFRLIQVNQLALKASDSLLNLAEQYPKDVLDAHSAQQSPLETQYDYLTLHYAGVISPQLFCN
QVLQHPLIEFKQFKINQIIEIDGTVQLLSSEQAILESNAAIVCCARESGKLFEQYPTLKPIRGQVSWFEQHTFPFAPDQA
FSYGGYCMQLHPQQLILGASFHPQRDDDEVLLEDHVHNFNLIHSVFPAYAQTLPPVEEWHGRASVRAQSPDYFPLVGQLQ
DHSKLFTLAGLGSKGYLFAPLNSEILVAHILGEACPVSANLLQKLNPQRFLKKVKIKKPYYSS

Sequences:

>Translated_623_residues
MCVSSSIQTAILDWQCVDGIDIPVSRQFGDVYFSKDNGLLETRHVFLNGNNLTERLADLKPYQYFCVGETGFGTGLNILA
VWQLWQQVRPDNHSHLHVISVEKFPLSKSDLERALRAWPELIILAEQLIEQYPFPIAGCHRLNFPDERFSLDLWLGDAHD
VFPSIVKTHSVDAWFLDGFAPSCNPELWETQVLNQIVRLSDYGTTFASFSVAGILKRGLKAHGIHISRPRGFGHKREMLK
AIWPFPDHESTTTVKGNQPSTQQHFAVIGAGIAGLHCAWSLAQRGHRVTLVDQSQPLSGASGNPLALLNPKLCPITQCHD
HLMVLCWQYARRFYQNFEAFRLIQVNQLALKASDSLLNLAEQYPKDVLDAHSAQQSPLETQYDYLTLHYAGVISPQLFCN
QVLQHPLIEFKQFKINQIIEIDGTVQLLSSEQAILESNAAIVCCARESGKLFEQYPTLKPIRGQVSWFEQHTFPFAPDQA
FSYGGYCMQLHPQQLILGASFHPQRDDDEVLLEDHVHNFNLIHSVFPAYAQTLPPVEEWHGRASVRAQSPDYFPLVGQLQ
DHSKLFTLAGLGSKGYLFAPLNSEILVAHILGEACPVSANLLQKLNPQRFLKKVKIKKPYYSS
>Mature_623_residues
MCVSSSIQTAILDWQCVDGIDIPVSRQFGDVYFSKDNGLLETRHVFLNGNNLTERLADLKPYQYFCVGETGFGTGLNILA
VWQLWQQVRPDNHSHLHVISVEKFPLSKSDLERALRAWPELIILAEQLIEQYPFPIAGCHRLNFPDERFSLDLWLGDAHD
VFPSIVKTHSVDAWFLDGFAPSCNPELWETQVLNQIVRLSDYGTTFASFSVAGILKRGLKAHGIHISRPRGFGHKREMLK
AIWPFPDHESTTTVKGNQPSTQQHFAVIGAGIAGLHCAWSLAQRGHRVTLVDQSQPLSGASGNPLALLNPKLCPITQCHD
HLMVLCWQYARRFYQNFEAFRLIQVNQLALKASDSLLNLAEQYPKDVLDAHSAQQSPLETQYDYLTLHYAGVISPQLFCN
QVLQHPLIEFKQFKINQIIEIDGTVQLLSSEQAILESNAAIVCCARESGKLFEQYPTLKPIRGQVSWFEQHTFPFAPDQA
FSYGGYCMQLHPQQLILGASFHPQRDDDEVLLEDHVHNFNLIHSVFPAYAQTLPPVEEWHGRASVRAQSPDYFPLVGQLQ
DHSKLFTLAGLGSKGYLFAPLNSEILVAHILGEACPVSANLLQKLNPQRFLKKVKIKKPYYSS

Specific function: Catalyzes the last two steps in the biosynthesis of 5- methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at the wobble position (U34) in tRNA. Catalyzes the FAD-dependent demodification of cmnm(5)s(2)U34 to nm(5)s(2)U34, followed by the transfer of a methyl g

COG id: COG4121

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the DAO family

Homologues:

Organism=Escherichia coli, GI87082084, Length=675, Percent_Identity=33.3333333333333, Blast_Score=293, Evalue=2e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNMC_ACIAD (Q6F7Y9)

Other databases:

- EMBL:   CR543861
- RefSeq:   YP_047648.1
- ProteinModelPortal:   Q6F7Y9
- SMR:   Q6F7Y9
- STRING:   Q6F7Y9
- GeneID:   2880432
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD3135
- NMPDR:   fig|62977.3.peg.2841
- eggNOG:   COG0665
- HOGENOM:   HBG677640
- OMA:   WLILPHL
- PhylomeDB:   Q6F7Y9
- ProtClustDB:   CLSK830736
- BioCyc:   ASP62977:ACIAD3135-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01102
- InterPro:   IPR008471
- InterPro:   IPR006076
- InterPro:   IPR023032
- InterPro:   IPR017610
- TIGRFAMs:   TIGR03197

Pfam domain/function: PF01266 DAO; PF05430 DUF752

EC number: =2.1.1.61

Molecular weight: Translated: 70281; Mature: 70281

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCVSSSIQTAILDWQCVDGIDIPVSRQFGDVYFSKDNGLLETRHVFLNGNNLTERLADLK
CCCCCCCHHEEECEEECCCCCCCCCCCCCCEEEECCCCEEEEEEEEEECCCHHHHHHCCC
PYQYFCVGETGFGTGLNILAVWQLWQQVRPDNHSHLHVISVEKFPLSKSDLERALRAWPE
CCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHH
LIILAEQLIEQYPFPIAGCHRLNFPDERFSLDLWLGDAHDVFPSIVKTHSVDAWFLDGFA
HHHHHHHHHHHCCCCCCCEEECCCCCCCEEEEEEECCHHHHHHHHHHHCCCCEEEECCCC
PSCNPELWETQVLNQIVRLSDYGTTFASFSVAGILKRGLKAHGIHISRPRGFGHKREMLK
CCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHCCEEEECCCCCCHHHHHHH
AIWPFPDHESTTTVKGNQPSTQQHFAVIGAGIAGLHCAWSLAQRGHRVTLVDQSQPLSGA
HHCCCCCCCCCEEEECCCCCCHHHHHHEECCHHHHHHHHHHHHCCCEEEEEECCCCCCCC
SGNPLALLNPKLCPITQCHDHLMVLCWQYARRFYQNFEAFRLIQVNQLALKASDSLLNLA
CCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHEEEEEEHHHHHHHHHHHHHHH
EQYPKDVLDAHSAQQSPLETQYDYLTLHYAGVISPQLFCNQVLQHPLIEFKQFKINQIIE
HHCCHHHHHHHCCCCCCCCCCCCEEEEEEHHHCCHHHHHHHHHHHHHHHHHHCCCCEEEE
IDGTVQLLSSEQAILESNAAIVCCARESGKLFEQYPTLKPIRGQVSWFEQHTFPFAPDQA
ECCEEEECCCCHHHHCCCCEEEEEECCCCCHHHHCCCCCCCCCCHHHHHHCCCCCCCCCC
FSYGGYCMQLHPQQLILGASFHPQRDDDEVLLEDHVHNFNLIHSVFPAYAQTLPPVEEWH
CCCCCEEEEECHHHEEEECCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHC
GRASVRAQSPDYFPLVGQLQDHSKLFTLAGLGSKGYLFAPLNSEILVAHILGEACPVSAN
CCCEEECCCCCCCCCCCCCCCCCEEEEEEECCCCCEEEECCCCCEEEEEHHCCCCCCCHH
LLQKLNPQRFLKKVKIKKPYYSS
HHHHCCHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MCVSSSIQTAILDWQCVDGIDIPVSRQFGDVYFSKDNGLLETRHVFLNGNNLTERLADLK
CCCCCCCHHEEECEEECCCCCCCCCCCCCCEEEECCCCEEEEEEEEEECCCHHHHHHCCC
PYQYFCVGETGFGTGLNILAVWQLWQQVRPDNHSHLHVISVEKFPLSKSDLERALRAWPE
CCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHH
LIILAEQLIEQYPFPIAGCHRLNFPDERFSLDLWLGDAHDVFPSIVKTHSVDAWFLDGFA
HHHHHHHHHHHCCCCCCCEEECCCCCCCEEEEEEECCHHHHHHHHHHHCCCCEEEECCCC
PSCNPELWETQVLNQIVRLSDYGTTFASFSVAGILKRGLKAHGIHISRPRGFGHKREMLK
CCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHCCEEEECCCCCCHHHHHHH
AIWPFPDHESTTTVKGNQPSTQQHFAVIGAGIAGLHCAWSLAQRGHRVTLVDQSQPLSGA
HHCCCCCCCCCEEEECCCCCCHHHHHHEECCHHHHHHHHHHHHCCCEEEEEECCCCCCCC
SGNPLALLNPKLCPITQCHDHLMVLCWQYARRFYQNFEAFRLIQVNQLALKASDSLLNLA
CCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHEEEEEEHHHHHHHHHHHHHHH
EQYPKDVLDAHSAQQSPLETQYDYLTLHYAGVISPQLFCNQVLQHPLIEFKQFKINQIIE
HHCCHHHHHHHCCCCCCCCCCCCEEEEEEHHHCCHHHHHHHHHHHHHHHHHHCCCCEEEE
IDGTVQLLSSEQAILESNAAIVCCARESGKLFEQYPTLKPIRGQVSWFEQHTFPFAPDQA
ECCEEEECCCCHHHHCCCCEEEEEECCCCCHHHHCCCCCCCCCCHHHHHHCCCCCCCCCC
FSYGGYCMQLHPQQLILGASFHPQRDDDEVLLEDHVHNFNLIHSVFPAYAQTLPPVEEWH
CCCCCEEEEECHHHEEEECCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHC
GRASVRAQSPDYFPLVGQLQDHSKLFTLAGLGSKGYLFAPLNSEILVAHILGEACPVSAN
CCCEEECCCCCCCCCCCCCCCCCEEEEEEECCCCCEEEECCCCCEEEEEHHCCCCCCCHH
LLQKLNPQRFLKKVKIKKPYYSS
HHHHCCHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA