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The map label for this gene is pntA-1 [H]

Identifier: 50086091

GI number: 50086091

Start: 3006647

End: 3007774

Strand: Reverse

Name: pntA-1 [H]

Synonym: ACIAD3080

Alternate gene names: 50086091

Gene position: 3007774-3006647 (Counterclockwise)

Preceding gene: 50086093

Following gene: 50086090

Centisome position: 83.58

GC content: 46.1

Gene sequence:

>1128_bases
ATGCAGATCGGAATCCCAACAGAAACGATCGCTGGTGAAAATCGTGTTGCTGCGACACCAGAGACTGTTAAAAAGTTGAT
TAGTGCTGGCCATCATGTCGTTATTGAACGTGGAGCAGGGGTAAAAGCTGCTTACATCGATAGTGCTTATGAACAGGTGG
GCGCCACCATTACGGATAATGCCTATCAAGGTAGCCAATTGATTTTGAAAGTTCGTGCACCACACAGTGACGAAATTCAA
AAGCTTTCAGCCAATACGACCGTTGTGGCAATGTTTGATCCTTATCGTAATCCTGATCTGGATGCATTTGCGGATCAAAA
TGTGTCTGCATTTGCGCTAGAGCTATTACCACGTACCTTGTCGCGTGCACAAAATATGGATGTTTTATCCTCTCAAGCCA
ACTTGGCTGGATACAAATCTGTATTACTCGCTGCTGCTGAATATCAACGTATGTTCCCAATGCTCATGACCGCAGCAGGT
ACAGTAAAACCTGCACGTGTGGTGATTATGGGCGTTGGTGTTGCAGGTCTTCAAGCGATTGCAACTGCAAAGCGTTTGGG
TGCAGTGGTTGAAGCAACCGATTTGCGTCCAACGGCTAAAGAGCAAGTCGAATCGCTTGGGGGGAAGTGGTTAGACGTGC
CAATGTCACCTGAAGAGCAAGCGCGTGCCGATGAAGCTGCCAAAAATGGATATGGCTGGACACCAGGTGAGCAGTACATC
AAGGATCAGGCAGCCATTGTCGATAAAGCCGTATCCAATGCCGATATCGTCATTACGACTGCGCTATTACCGGGTCGAAA
TGCACCGCGTCTGATTAAAGCGGAAACGGTGGCCAAAATGAAACCAGGTTCGGTGATTTTGGATATGGCAGTTGAAACAG
GTGGTAACGTTGAAGGTTCTAAAGTCGGCGAAACGGTATTCACCGAAAATGGTGTGAAGATTTTAGGTGTTCCCAATATT
CCATCGACCTTGTCGACAGAAGCTTCAGCACTTTATGCACGTAATGTGTTCAATTTTGTTGAAACACTGTTTGATCAAGA
CAAAAATTTTGCCATCAATCCAGAAGAAGAAATTCAGAAAGCCCTATTGGTCACTCATGGCGGTCAAGTGCTGCTTAAGC
GTGGTTAA

Upstream 100 bases:

>100_bases
AAGTTCAATTAGAATCCAAAAAATGAACAAAACACAACCTGGGGGAGAACATCGTTTTTTACGATGTTTGTACTTAAGAA
AATTAGCTTGAGGAACGCTC

Downstream 100 bases:

>100_bases
GGAGAATACTCATGGTTGAAACGATTACAATTTTCGTCCTTGCCATCTTCGTAGGTTATTACGTGGTATGGGGGGTAACA
CCTGCATTACATACGCCGCT

Product: pyridine nucleotide transhydrogenase (proton pump), alpha subunit (part1)

Products: NA

Alternate protein names: Nicotinamide nucleotide transhydrogenase subunit alpha 1; Proton-translocating transhydrogenase component 1; Pyridine nucleotide transhydrogenase subunit alpha 1; dI [H]

Number of amino acids: Translated: 375; Mature: 375

Protein sequence:

>375_residues
MQIGIPTETIAGENRVAATPETVKKLISAGHHVVIERGAGVKAAYIDSAYEQVGATITDNAYQGSQLILKVRAPHSDEIQ
KLSANTTVVAMFDPYRNPDLDAFADQNVSAFALELLPRTLSRAQNMDVLSSQANLAGYKSVLLAAAEYQRMFPMLMTAAG
TVKPARVVIMGVGVAGLQAIATAKRLGAVVEATDLRPTAKEQVESLGGKWLDVPMSPEEQARADEAAKNGYGWTPGEQYI
KDQAAIVDKAVSNADIVITTALLPGRNAPRLIKAETVAKMKPGSVILDMAVETGGNVEGSKVGETVFTENGVKILGVPNI
PSTLSTEASALYARNVFNFVETLFDQDKNFAINPEEEIQKALLVTHGGQVLLKRG

Sequences:

>Translated_375_residues
MQIGIPTETIAGENRVAATPETVKKLISAGHHVVIERGAGVKAAYIDSAYEQVGATITDNAYQGSQLILKVRAPHSDEIQ
KLSANTTVVAMFDPYRNPDLDAFADQNVSAFALELLPRTLSRAQNMDVLSSQANLAGYKSVLLAAAEYQRMFPMLMTAAG
TVKPARVVIMGVGVAGLQAIATAKRLGAVVEATDLRPTAKEQVESLGGKWLDVPMSPEEQARADEAAKNGYGWTPGEQYI
KDQAAIVDKAVSNADIVITTALLPGRNAPRLIKAETVAKMKPGSVILDMAVETGGNVEGSKVGETVFTENGVKILGVPNI
PSTLSTEASALYARNVFNFVETLFDQDKNFAINPEEEIQKALLVTHGGQVLLKRG
>Mature_375_residues
MQIGIPTETIAGENRVAATPETVKKLISAGHHVVIERGAGVKAAYIDSAYEQVGATITDNAYQGSQLILKVRAPHSDEIQ
KLSANTTVVAMFDPYRNPDLDAFADQNVSAFALELLPRTLSRAQNMDVLSSQANLAGYKSVLLAAAEYQRMFPMLMTAAG
TVKPARVVIMGVGVAGLQAIATAKRLGAVVEATDLRPTAKEQVESLGGKWLDVPMSPEEQARADEAAKNGYGWTPGEQYI
KDQAAIVDKAVSNADIVITTALLPGRNAPRLIKAETVAKMKPGSVILDMAVETGGNVEGSKVGETVFTENGVKILGVPNI
PSTLSTEASALYARNVFNFVETLFDQDKNFAINPEEEIQKALLVTHGGQVLLKRG

Specific function: The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane [H]

COG id: COG3288

COG function: function code C; NAD/NADP transhydrogenase alpha subunit

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AlaDH/PNT family [H]

Homologues:

Organism=Homo sapiens, GI122939155, Length=366, Percent_Identity=34.9726775956284, Blast_Score=222, Evalue=5e-58,
Organism=Homo sapiens, GI122939153, Length=366, Percent_Identity=34.9726775956284, Blast_Score=222, Evalue=5e-58,
Organism=Escherichia coli, GI1787887, Length=373, Percent_Identity=42.627345844504, Blast_Score=287, Evalue=6e-79,
Organism=Caenorhabditis elegans, GI17550456, Length=372, Percent_Identity=38.9784946236559, Blast_Score=260, Evalue=7e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007698
- InterPro:   IPR008142
- InterPro:   IPR008143
- InterPro:   IPR007886 [H]

Pfam domain/function: PF01262 AlaDh_PNT_C; PF05222 AlaDh_PNT_N [H]

EC number: =1.6.1.2 [H]

Molecular weight: Translated: 39957; Mature: 39957

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: PS00836 ALADH_PNT_1 ; PS00837 ALADH_PNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIGIPTETIAGENRVAATPETVKKLISAGHHVVIERGAGVKAAYIDSAYEQVGATITDN
CCCCCCCHHCCCCCCEECCHHHHHHHHHCCCEEEEECCCCCEEHHHHHHHHHHCCEEECC
AYQGSQLILKVRAPHSDEIQKLSANTTVVAMFDPYRNPDLDAFADQNVSAFALELLPRTL
CCCCCEEEEEEECCCCHHHHHHCCCCEEEEEECCCCCCCCHHHCCCCHHHHHHHHHHHHH
SRAQNMDVLSSQANLAGYKSVLLAAAEYQRMFPMLMTAAGTVKPARVVIMGVGVAGLQAI
HHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCHHHHHHH
ATAKRLGAVVEATDLRPTAKEQVESLGGKWLDVPMSPEEQARADEAAKNGYGWTPGEQYI
HHHHHHHHHEEECCCCCCHHHHHHHCCCCEEECCCCCHHHHHHHHHHHCCCCCCCHHHHH
KDQAAIVDKAVSNADIVITTALLPGRNAPRLIKAETVAKMKPGSVILDMAVETGGNVEGS
HHHHHHHHHHCCCCCEEEEEEECCCCCCCCEEEHHHHHHCCCCCEEEEEEECCCCCCCCC
KVGETVFTENGVKILGVPNIPSTLSTEASALYARNVFNFVETLFDQDKNFAINPEEEIQK
CCCCEEEECCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
ALLVTHGGQVLLKRG
HHHHCCCCEEEEECC
>Mature Secondary Structure
MQIGIPTETIAGENRVAATPETVKKLISAGHHVVIERGAGVKAAYIDSAYEQVGATITDN
CCCCCCCHHCCCCCCEECCHHHHHHHHHCCCEEEEECCCCCEEHHHHHHHHHHCCEEECC
AYQGSQLILKVRAPHSDEIQKLSANTTVVAMFDPYRNPDLDAFADQNVSAFALELLPRTL
CCCCCEEEEEEECCCCHHHHHHCCCCEEEEEECCCCCCCCHHHCCCCHHHHHHHHHHHHH
SRAQNMDVLSSQANLAGYKSVLLAAAEYQRMFPMLMTAAGTVKPARVVIMGVGVAGLQAI
HHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCHHHHHHH
ATAKRLGAVVEATDLRPTAKEQVESLGGKWLDVPMSPEEQARADEAAKNGYGWTPGEQYI
HHHHHHHHHEEECCCCCCHHHHHHHCCCCEEECCCCCHHHHHHHHHHHCCCCCCCHHHHH
KDQAAIVDKAVSNADIVITTALLPGRNAPRLIKAETVAKMKPGSVILDMAVETGGNVEGS
HHHHHHHHHHCCCCCEEEEEEECCCCCCCCEEEHHHHHHCCCCCEEEEEEECCCCCCCCC
KVGETVFTENGVKILGVPNIPSTLSTEASALYARNVFNFVETLFDQDKNFAINPEEEIQK
CCCCEEEECCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
ALLVTHGGQVLLKRG
HHHHCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8075801 [H]