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The map label for this gene is pheS

Identifier: 50086055

GI number: 50086055

Start: 2972798

End: 2973790

Strand: Reverse

Name: pheS

Synonym: ACIAD3042

Alternate gene names: 50086055

Gene position: 2973790-2972798 (Counterclockwise)

Preceding gene: 50086057

Following gene: 50086054

Centisome position: 82.64

GC content: 43.81

Gene sequence:

>993_bases
TTGAGAGTTACTATGTCACTGGAAGCCCTGACCACCGAAGCGCTTGCTGCTATAGCAGCAGCTCAAGACCTTGCTGCACT
CGATCAAGTTAGAGTTCTGTTTACAGGGAAAAAAAGCCAGCTTGCAGAACAATCGAAAGCATTAGGAAAAATGGATCCTG
AGCAACGTAAAGTGCAAGGTGCAGCCATTCATGCTGTCCGTGAAGCAATTAACGCTGCCTTAACTGAGCGTCAGACTGCA
TTGCAGCAAGCCGCATTACAACAAAAACTGGCAAGTGAAACCATCGATATTACGCTGCCTGGGCGTGGTCAGCATATGGG
CAGTATTCACCCAGTTACTCAAGTACAAGAACGTATTTGCCAATTTTTTACCAAAGCAGGCTTTAGTGTCGCGACAGGGC
CAGAAGTTGAAGATGACTACCACAACTTTGAAGCATTGAATATTCCAGGTCATCATCCTGCGCGTGCCATGCACGATACA
TTCTATTTTGATGTGAATCATTTATTGCGTACACATACCTCTGGTGTACAGATTCGTACAATGGAAACCACGCAACCACC
AATTCGTATTGTGTGCCCAGGGCGTGTATATCGTTGTGATTCAGATCAGACACATTCTCCAATGTTCCATCAGATTGAAG
GTCTTTACGTTGCCGAAAATACCAGTTTTGCAGAGCTTAAAGGCCTGCTGATCAATCTATTGAATGAATTTTTTGAAAAA
GACCTCAAGGTCCGTTTCCGTCCATCTTACTTTCCGTTTACAGAACCAAGTGCCGAAGTGGATATTATGGATGAGCGTGG
TCGCTGGTTAGAAGTTTTAGGCTGTGGCATGGTACATCCAAATGTATTACGTGCTGCTGGCATTGATCCTGAAAAATATA
AAGGTTTTGCCTTTGGTTTAGGGGTAGAGCGTTTTGCAATGTTGCGTTATGGCATCAATGATTTGCGAATGTTCTATCAA
AATGATGTGCGTTTCTTACGCCAATTTGCTTAA

Upstream 100 bases:

>100_bases
TATAAATCTACTGCCTAGTAATTTTCCTGTGTTTATGCTTCATCAATCCATTTAAAAACTGTTAGAATGCAGAGTTTTAT
TTTATTTATAAATTGTTGCT

Downstream 100 bases:

>100_bases
ACATTTTCTGGATTAAATTTAAGGTTTTATAAAGATGAAAATTAGTGAAAGTTGGTTACGCACTTGGGTAAATCCTGCAA
TTGATAGCACAACATTGGCC

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS

Number of amino acids: Translated: 330; Mature: 330

Protein sequence:

>330_residues
MRVTMSLEALTTEALAAIAAAQDLAALDQVRVLFTGKKSQLAEQSKALGKMDPEQRKVQGAAIHAVREAINAALTERQTA
LQQAALQQKLASETIDITLPGRGQHMGSIHPVTQVQERICQFFTKAGFSVATGPEVEDDYHNFEALNIPGHHPARAMHDT
FYFDVNHLLRTHTSGVQIRTMETTQPPIRIVCPGRVYRCDSDQTHSPMFHQIEGLYVAENTSFAELKGLLINLLNEFFEK
DLKVRFRPSYFPFTEPSAEVDIMDERGRWLEVLGCGMVHPNVLRAAGIDPEKYKGFAFGLGVERFAMLRYGINDLRMFYQ
NDVRFLRQFA

Sequences:

>Translated_330_residues
MRVTMSLEALTTEALAAIAAAQDLAALDQVRVLFTGKKSQLAEQSKALGKMDPEQRKVQGAAIHAVREAINAALTERQTA
LQQAALQQKLASETIDITLPGRGQHMGSIHPVTQVQERICQFFTKAGFSVATGPEVEDDYHNFEALNIPGHHPARAMHDT
FYFDVNHLLRTHTSGVQIRTMETTQPPIRIVCPGRVYRCDSDQTHSPMFHQIEGLYVAENTSFAELKGLLINLLNEFFEK
DLKVRFRPSYFPFTEPSAEVDIMDERGRWLEVLGCGMVHPNVLRAAGIDPEKYKGFAFGLGVERFAMLRYGINDLRMFYQ
NDVRFLRQFA
>Mature_330_residues
MRVTMSLEALTTEALAAIAAAQDLAALDQVRVLFTGKKSQLAEQSKALGKMDPEQRKVQGAAIHAVREAINAALTERQTA
LQQAALQQKLASETIDITLPGRGQHMGSIHPVTQVQERICQFFTKAGFSVATGPEVEDDYHNFEALNIPGHHPARAMHDT
FYFDVNHLLRTHTSGVQIRTMETTQPPIRIVCPGRVYRCDSDQTHSPMFHQIEGLYVAENTSFAELKGLLINLLNEFFEK
DLKVRFRPSYFPFTEPSAEVDIMDERGRWLEVLGCGMVHPNVLRAAGIDPEKYKGFAFGLGVERFAMLRYGINDLRMFYQ
NDVRFLRQFA

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily

Homologues:

Organism=Homo sapiens, GI5729820, Length=279, Percent_Identity=33.6917562724014, Blast_Score=143, Evalue=2e-34,
Organism=Homo sapiens, GI4758340, Length=259, Percent_Identity=32.046332046332, Blast_Score=118, Evalue=6e-27,
Organism=Escherichia coli, GI1788007, Length=323, Percent_Identity=63.1578947368421, Blast_Score=441, Evalue=1e-125,
Organism=Caenorhabditis elegans, GI32566635, Length=275, Percent_Identity=36.3636363636364, Blast_Score=148, Evalue=4e-36,
Organism=Caenorhabditis elegans, GI32563657, Length=265, Percent_Identity=31.6981132075472, Blast_Score=122, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17508957, Length=265, Percent_Identity=31.6981132075472, Blast_Score=122, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6321087, Length=258, Percent_Identity=29.8449612403101, Blast_Score=120, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6325304, Length=91, Percent_Identity=47.2527472527472, Blast_Score=98, Evalue=2e-21,
Organism=Drosophila melanogaster, GI17137424, Length=288, Percent_Identity=30.9027777777778, Blast_Score=133, Evalue=1e-31,
Organism=Drosophila melanogaster, GI18858079, Length=262, Percent_Identity=32.0610687022901, Blast_Score=114, Evalue=9e-26,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYFA_ACIAD (Q6F872)

Other databases:

- EMBL:   CR543861
- RefSeq:   YP_047565.1
- ProteinModelPortal:   Q6F872
- SMR:   Q6F872
- STRING:   Q6F872
- GeneID:   2880188
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD3042
- NMPDR:   fig|62977.3.peg.2758
- eggNOG:   COG0016
- HOGENOM:   HBG284353
- OMA:   FRASYFP
- PhylomeDB:   Q6F872
- ProtClustDB:   PRK00488
- BioCyc:   ASP62977:ACIAD3042-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00281
- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978
- PANTHER:   PTHR11538
- TIGRFAMs:   TIGR00468

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d; SSF46589 tRNA_binding_arm

EC number: =6.1.1.20

Molecular weight: Translated: 37154; Mature: 37154

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVTMSLEALTTEALAAIAAAQDLAALDQVRVLFTGKKSQLAEQSKALGKMDPEQRKVQG
CEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCHHHHHHHHHHHCCCCHHHHHHHH
AAIHAVREAINAALTERQTALQQAALQQKLASETIDITLPGRGQHMGSIHPVTQVQERIC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCCCCCCHHHHHHHHH
QFFTKAGFSVATGPEVEDDYHNFEALNIPGHHPARAMHDTFYFDVNHLLRTHTSGVQIRT
HHHHHCCCCCCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEE
METTQPPIRIVCPGRVYRCDSDQTHSPMFHQIEGLYVAENTSFAELKGLLINLLNEFFEK
EECCCCCEEEECCCEEEECCCCCCCCCHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHHC
DLKVRFRPSYFPFTEPSAEVDIMDERGRWLEVLGCGMVHPNVLRAAGIDPEKYKGFAFGL
CCEEEECCCCCCCCCCCCCEEEECCCCCEEEHHCCCCCCCHHHHHCCCCHHHHCCEEEEC
GVERFAMLRYGINDLRMFYQNDVRFLRQFA
CHHHHHHHHHCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRVTMSLEALTTEALAAIAAAQDLAALDQVRVLFTGKKSQLAEQSKALGKMDPEQRKVQG
CEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCHHHHHHHHHHHCCCCHHHHHHHH
AAIHAVREAINAALTERQTALQQAALQQKLASETIDITLPGRGQHMGSIHPVTQVQERIC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCCCCCCHHHHHHHHH
QFFTKAGFSVATGPEVEDDYHNFEALNIPGHHPARAMHDTFYFDVNHLLRTHTSGVQIRT
HHHHHCCCCCCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEE
METTQPPIRIVCPGRVYRCDSDQTHSPMFHQIEGLYVAENTSFAELKGLLINLLNEFFEK
EECCCCCEEEECCCEEEECCCCCCCCCHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHHC
DLKVRFRPSYFPFTEPSAEVDIMDERGRWLEVLGCGMVHPNVLRAAGIDPEKYKGFAFGL
CCEEEECCCCCCCCCCCCCEEEECCCCCEEEHHCCCCCCCHHHHHCCCCHHHHCCEEEEC
GVERFAMLRYGINDLRMFYQNDVRFLRQFA
CHHHHHHHHHCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA