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The map label for this gene is rho [H]

Identifier: 50086051

GI number: 50086051

Start: 2967985

End: 2969253

Strand: Direct

Name: rho [H]

Synonym: ACIAD3038

Alternate gene names: 50086051

Gene position: 2967985-2969253 (Clockwise)

Preceding gene: 50086050

Following gene: 50086052

Centisome position: 82.48

GC content: 43.03

Gene sequence:

>1269_bases
ATGAATTTAACCGAACTCAAGAAAAAACCGATTGGCGAACTCATTAAGATTGCAGAGTTTATGGGCCTTGAAGGTATGGC
TCGTAACCGCAAGCAAGATATTATTTTTGCCATCTTAAAACGTCATGCAATGAACGGCGAAGAAATTTTTGGCGATGGTG
TGCTTGAAATTCTCTCTGATGGTTTTGGTTTCTTACGTTCAGCTGCAGGTTCTTACCTTGCAGGTCCAGACGATATCTAT
GTTAGCCCATCGCAAATTCGTCGTTTCAATTTGCGCACAGGTGACACTATTACAGGAACCATTCGTCCACCTAAAGAAGG
CGAACGTTATTTTGCATTATTAAAGGTTAATCAGATTAATTACGATACGCCAGAAAACTCGCGTAATAAGATTTTATTTG
AAAACCTCACACCGCTTTTCCCAACCGAACAACTCGTGATGGAACTAGGGAACGGTACCACAGAAGATTTAACGACGCGT
GTGGTCGACTTAATCGCACCAATCGGTAAAGGTCAGCGTTCGATTATCGTTGCACCACCCAAAGCTGGTAAAACCATGTT
GTTGCAAAGTATTGCGCAATCGATTGTTCGCAACAACCCAGAGGTGTTCCTGATTGTTCTTCTCATCGACGAGCGTCCAG
AAGAAGTCACCGAAATGGAACGTACTGTTCGTGGTGAAGTCGTTGCCTCAACATTTGATGAATCGCCTGCACGTCACGTT
CAAGTAGCAGAAATGGTGATTGAAAAAGCCAAACGTTTGGTTGAACACAAAAAAGACGTCGTAATTCTACTTGATTCGAT
TACCCGTCTGGCACGTGCTTATAACACTGTTATTCCATCATCTGGCAAAGTATTGACTGGTGGTGTGGATGCACATGCCC
TAGAGCGTCCTAAGCGCTTCTTTGGTGCTGCACGTAATATCGAAGAAGGTGGATCACTGACCATCATCTCGACTGCATTG
ATTGAAACTGGCAGTAAGATGGATGATGTCATTTATGAAGAATTCAAAGGAACAGGTAACCAAGAAATTACGTTAGACCG
CCGTATTGCCGAAAAGCGTGTGTTCCCTGCCATGAATATCAAAAAATCAGGTACACGTCGTGAAGAACGCCTGATGGATG
AAGATAACCTGCGCAAAGTCTGGATCCTGCGTAAGCTTTTACATCCAATGGATGAGTTGGCGGCAATGGAGTTCTTGCTT
GATCGTATGAAAGAAACCAAAACCAATGATGATTTCTTTGATCAAATGAAACGTAAAGCTGCAAACTAA

Upstream 100 bases:

>100_bases
GTGCAAGTGCGATTTTTATCGTCATTTGAATGCTTGCGGCTGACCTTTATATCTTTCCATTTCTGTGCTCTACACACTCC
TTTTATCTATCTGACCACTT

Downstream 100 bases:

>100_bases
GTTTCATCAAAACTATCAGAAGGCTGTCTATAACGACAGCCTTTTTCATTTGGTTGACTTTGATTACATAAATATACAAT
TGCAAAATCTGACTGTTAAA

Product: transcription termination factor Rho

Products: NA

Alternate protein names: ATP-dependent helicase Rho [H]

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MNLTELKKKPIGELIKIAEFMGLEGMARNRKQDIIFAILKRHAMNGEEIFGDGVLEILSDGFGFLRSAAGSYLAGPDDIY
VSPSQIRRFNLRTGDTITGTIRPPKEGERYFALLKVNQINYDTPENSRNKILFENLTPLFPTEQLVMELGNGTTEDLTTR
VVDLIAPIGKGQRSIIVAPPKAGKTMLLQSIAQSIVRNNPEVFLIVLLIDERPEEVTEMERTVRGEVVASTFDESPARHV
QVAEMVIEKAKRLVEHKKDVVILLDSITRLARAYNTVIPSSGKVLTGGVDAHALERPKRFFGAARNIEEGGSLTIISTAL
IETGSKMDDVIYEEFKGTGNQEITLDRRIAEKRVFPAMNIKKSGTRREERLMDEDNLRKVWILRKLLHPMDELAAMEFLL
DRMKETKTNDDFFDQMKRKAAN

Sequences:

>Translated_422_residues
MNLTELKKKPIGELIKIAEFMGLEGMARNRKQDIIFAILKRHAMNGEEIFGDGVLEILSDGFGFLRSAAGSYLAGPDDIY
VSPSQIRRFNLRTGDTITGTIRPPKEGERYFALLKVNQINYDTPENSRNKILFENLTPLFPTEQLVMELGNGTTEDLTTR
VVDLIAPIGKGQRSIIVAPPKAGKTMLLQSIAQSIVRNNPEVFLIVLLIDERPEEVTEMERTVRGEVVASTFDESPARHV
QVAEMVIEKAKRLVEHKKDVVILLDSITRLARAYNTVIPSSGKVLTGGVDAHALERPKRFFGAARNIEEGGSLTIISTAL
IETGSKMDDVIYEEFKGTGNQEITLDRRIAEKRVFPAMNIKKSGTRREERLMDEDNLRKVWILRKLLHPMDELAAMEFLL
DRMKETKTNDDFFDQMKRKAAN
>Mature_422_residues
MNLTELKKKPIGELIKIAEFMGLEGMARNRKQDIIFAILKRHAMNGEEIFGDGVLEILSDGFGFLRSAAGSYLAGPDDIY
VSPSQIRRFNLRTGDTITGTIRPPKEGERYFALLKVNQINYDTPENSRNKILFENLTPLFPTEQLVMELGNGTTEDLTTR
VVDLIAPIGKGQRSIIVAPPKAGKTMLLQSIAQSIVRNNPEVFLIVLLIDERPEEVTEMERTVRGEVVASTFDESPARHV
QVAEMVIEKAKRLVEHKKDVVILLDSITRLARAYNTVIPSSGKVLTGGVDAHALERPKRFFGAARNIEEGGSLTIISTAL
IETGSKMDDVIYEEFKGTGNQEITLDRRIAEKRVFPAMNIKKSGTRREERLMDEDNLRKVWILRKLLHPMDELAAMEFLL
DRMKETKTNDDFFDQMKRKAAN

Specific function: Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template [H]

COG id: COG1158

COG function: function code K; Transcription termination factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Rho family [H]

Homologues:

Organism=Escherichia coli, GI1790217, Length=418, Percent_Identity=77.2727272727273, Blast_Score=674, Evalue=0.0,
Organism=Escherichia coli, GI1788251, Length=241, Percent_Identity=28.6307053941909, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000194
- InterPro:   IPR003593
- InterPro:   IPR011129
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR011112
- InterPro:   IPR011113
- InterPro:   IPR004665 [H]

Pfam domain/function: PF00006 ATP-synt_ab; PF07498 Rho_N; PF07497 Rho_RNA_bind [H]

EC number: NA

Molecular weight: Translated: 47544; Mature: 47544

Theoretical pI: Translated: 7.11; Mature: 7.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLTELKKKPIGELIKIAEFMGLEGMARNRKQDIIFAILKRHAMNGEEIFGDGVLEILSD
CCHHHHHHCHHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHC
GFGFLRSAAGSYLAGPDDIYVSPSQIRRFNLRTGDTITGTIRPPKEGERYFALLKVNQIN
CHHHHHHHCCCCCCCCCCEEECHHHHHEEECCCCCEEEEECCCCCCCCEEEEEEEEEECC
YDTPENSRNKILFENLTPLFPTEQLVMELGNGTTEDLTTRVVDLIAPIGKGQRSIIVAPP
CCCCCCCCCEEEECCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCEEEEECC
KAGKTMLLQSIAQSIVRNNPEVFLIVLLIDERPEEVTEMERTVRGEVVASTFDESPARHV
CCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCHHHH
QVAEMVIEKAKRLVEHKKDVVILLDSITRLARAYNTVIPSSGKVLTGGVDAHALERPKRF
HHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHH
FGAARNIEEGGSLTIISTALIETGSKMDDVIYEEFKGTGNQEITLDRRIAEKRVFPAMNI
HHHHCCCCCCCCEEEEEHHHHHCCCHHHHHHHHHHCCCCCCEEEHHHHHHHHHCCCCCCC
KKSGTRREERLMDEDNLRKVWILRKLLHPMDELAAMEFLLDRMKETKTNDDFFDQMKRKA
CCCCCCHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
AN
CC
>Mature Secondary Structure
MNLTELKKKPIGELIKIAEFMGLEGMARNRKQDIIFAILKRHAMNGEEIFGDGVLEILSD
CCHHHHHHCHHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHC
GFGFLRSAAGSYLAGPDDIYVSPSQIRRFNLRTGDTITGTIRPPKEGERYFALLKVNQIN
CHHHHHHHCCCCCCCCCCEEECHHHHHEEECCCCCEEEEECCCCCCCCEEEEEEEEEECC
YDTPENSRNKILFENLTPLFPTEQLVMELGNGTTEDLTTRVVDLIAPIGKGQRSIIVAPP
CCCCCCCCCEEEECCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCEEEEECC
KAGKTMLLQSIAQSIVRNNPEVFLIVLLIDERPEEVTEMERTVRGEVVASTFDESPARHV
CCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCHHHH
QVAEMVIEKAKRLVEHKKDVVILLDSITRLARAYNTVIPSSGKVLTGGVDAHALERPKRF
HHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHH
FGAARNIEEGGSLTIISTALIETGSKMDDVIYEEFKGTGNQEITLDRRIAEKRVFPAMNI
HHHHCCCCCCCCEEEEEHHHHHCCCHHHHHHHHHHCCCCCCEEEHHHHHHHHHCCCCCCC
KKSGTRREERLMDEDNLRKVWILRKLLHPMDELAAMEFLLDRMKETKTNDDFFDQMKRKA
CCCCCCHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
AN
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]