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The map label for this gene is oprM [H]

Identifier: 50085972

GI number: 50085972

Start: 2876603

End: 2878057

Strand: Direct

Name: oprM [H]

Synonym: ACIAD2945

Alternate gene names: 50085972

Gene position: 2876603-2878057 (Clockwise)

Preceding gene: 50085971

Following gene: 50085973

Centisome position: 79.94

GC content: 43.44

Gene sequence:

>1455_bases
GTGATGCAAAAAGTATGGTCTATTTCAGGTCGTAGCATTGCGGTATCTGCACTTGCGCTTGCTTTGGCTGCATGCCAAAG
CATGCGTGGCCCAGAACCAGTTGTCAAAGCCGACATTCCGACAAGTTATACCTATAACGCGTCAGGTCAATCTATTGCAG
AGCAAGGCTATAAAGACTTTTTCTCTGACCCTCGCTTATTGCAAGTGATTGAGTTGGCTCTCAACAATAACCGTGATCTA
CGTACCGCTGCATTGAATATTCAGCGTGCACAACAACAGTATCAAATTACTGAAAATAACCAGCTTCCAACAATTGGAGC
AAGTGGTAGTGCAATCCGTCAGGTAAGCATCACACGTGATCCCAATAATCCATACTCCACGTTTCAAGTTGGACTAGGCG
TGACTGCCTATGAGCTTGATTTCTGGGGGCGAGTACGCAGTCTGAAGGATGCAGCACTCGATAATTATCTGGCGACACAA
AGCACACGTGACACCACTCAGATCAGTCTAGTGAGTCAGGTGGCTCAGGCGTGGTTAAGCTACTCTTTCGCCGTTGCAAA
TTTGAAGCTGGCTGATCAAACACTGAAAGCTCAACTCGATTCTTATAATCTGAACAAAAAACGTTTTGATGTAGGTATTG
ATAGTGAAGTTCCTTTACGTCAAGCTCAGATTTCGGTCGAAACTGCACGTAACGATGTAGCCAACTATAAAACGCAAATC
GTTCAGGCTCAGAACTTACTCAATCTTTTAGTAGGTCAGTCTGTTCCTCAGAATTTGCTACCGAATCAACCTGTCAAATC
TATTACACGTCAAAATGTATTTAGTACAGGCTTACCAAGTGACTTGTTAAACAATCGTCCAGATGTAAAATCTGCGGAAT
ATCAACTGAGTGCGGCAGGTGCCAATATTGGTGCTGCCAAAGCTGCGTTATTTCCAACAATTAGCTTAACAGGTTCAGCA
GGCTATGCATCTACACAATTAAGCGACCTGTTTAAATCTGGCGCTGGTGTCTGGTCAATTGGCCCAAGTCTTGAATTACC
AATTTTTGATTGGGGTACGCGTCGTGCCAATATCAAAATTTCTGAAACTGACCAAAAAATTGCTCTTTCTAATTATGAAA
AGTCAATTCAGTCAGCTTTCCGTGAAGTGAATGATGCTTTAGCAACGCGTGCCAATATTGGTGACCGTATCTCTGCCCAG
CGCCGTCTGGTAGATGCAACTAATACCACATATAGCTTATCAAATGCCCGCTTCCGTGCAGGTATCGATAACTATTTGAC
TGTATTAGATGCACAACGTACCTCCTACTCGGCTGAACAAGGTCTTTTACTGCTTGAACAAGCGAATCTGAATAACCAGA
TTGAACTCTACAAAACCTTAGGTGGTGGTCTAAAAGCCAACACCACGGATACTGTGGTTCATCAGCCTTCAAGTGCAGAT
CTGAAAAAGAAATAA

Upstream 100 bases:

>100_bases
CAACGCTTTTAGGTATCTTCTTTATCCCAATTTTCTTTGTGTGGATTCGGGGTGTCTTTAAGTACAAACCAAAAACCGTT
AATAATCAGGAGCACAGATC

Downstream 100 bases:

>100_bases
CGATTTATTCGATACTTAAAGCTCACTTCGGTGGGCTTTTTTATTGGAGATTTTCATTGCATTGACGATGCTTTTTTCAT
ATCTTGAATGGACATTCATT

Product: major intrinsic multiple antibiotic resistance efflux outer membrane protein precursor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 484; Mature: 484

Protein sequence:

>484_residues
MMQKVWSISGRSIAVSALALALAACQSMRGPEPVVKADIPTSYTYNASGQSIAEQGYKDFFSDPRLLQVIELALNNNRDL
RTAALNIQRAQQQYQITENNQLPTIGASGSAIRQVSITRDPNNPYSTFQVGLGVTAYELDFWGRVRSLKDAALDNYLATQ
STRDTTQISLVSQVAQAWLSYSFAVANLKLADQTLKAQLDSYNLNKKRFDVGIDSEVPLRQAQISVETARNDVANYKTQI
VQAQNLLNLLVGQSVPQNLLPNQPVKSITRQNVFSTGLPSDLLNNRPDVKSAEYQLSAAGANIGAAKAALFPTISLTGSA
GYASTQLSDLFKSGAGVWSIGPSLELPIFDWGTRRANIKISETDQKIALSNYEKSIQSAFREVNDALATRANIGDRISAQ
RRLVDATNTTYSLSNARFRAGIDNYLTVLDAQRTSYSAEQGLLLLEQANLNNQIELYKTLGGGLKANTTDTVVHQPSSAD
LKKK

Sequences:

>Translated_484_residues
MMQKVWSISGRSIAVSALALALAACQSMRGPEPVVKADIPTSYTYNASGQSIAEQGYKDFFSDPRLLQVIELALNNNRDL
RTAALNIQRAQQQYQITENNQLPTIGASGSAIRQVSITRDPNNPYSTFQVGLGVTAYELDFWGRVRSLKDAALDNYLATQ
STRDTTQISLVSQVAQAWLSYSFAVANLKLADQTLKAQLDSYNLNKKRFDVGIDSEVPLRQAQISVETARNDVANYKTQI
VQAQNLLNLLVGQSVPQNLLPNQPVKSITRQNVFSTGLPSDLLNNRPDVKSAEYQLSAAGANIGAAKAALFPTISLTGSA
GYASTQLSDLFKSGAGVWSIGPSLELPIFDWGTRRANIKISETDQKIALSNYEKSIQSAFREVNDALATRANIGDRISAQ
RRLVDATNTTYSLSNARFRAGIDNYLTVLDAQRTSYSAEQGLLLLEQANLNNQIELYKTLGGGLKANTTDTVVHQPSSAD
LKKK
>Mature_484_residues
MMQKVWSISGRSIAVSALALALAACQSMRGPEPVVKADIPTSYTYNASGQSIAEQGYKDFFSDPRLLQVIELALNNNRDL
RTAALNIQRAQQQYQITENNQLPTIGASGSAIRQVSITRDPNNPYSTFQVGLGVTAYELDFWGRVRSLKDAALDNYLATQ
STRDTTQISLVSQVAQAWLSYSFAVANLKLADQTLKAQLDSYNLNKKRFDVGIDSEVPLRQAQISVETARNDVANYKTQI
VQAQNLLNLLVGQSVPQNLLPNQPVKSITRQNVFSTGLPSDLLNNRPDVKSAEYQLSAAGANIGAAKAALFPTISLTGSA
GYASTQLSDLFKSGAGVWSIGPSLELPIFDWGTRRANIKISETDQKIALSNYEKSIQSAFREVNDALATRANIGDRISAQ
RRLVDATNTTYSLSNARFRAGIDNYLTVLDAQRTSYSAEQGLLLLEQANLNNQIELYKTLGGGLKANTTDTVVHQPSSAD
LKKK

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=456, Percent_Identity=37.719298245614, Blast_Score=280, Evalue=1e-76,
Organism=Escherichia coli, GI2367348, Length=420, Percent_Identity=24.7619047619048, Blast_Score=120, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 52780; Mature: 52780

Theoretical pI: Translated: 9.47; Mature: 9.47

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMQKVWSISGRSIAVSALALALAACQSMRGPEPVVKADIPTSYTYNASGQSIAEQGYKDF
CCCCHHCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCEEECCCCHHHHHHHHHHH
FSDPRLLQVIELALNNNRDLRTAALNIQRAQQQYQITENNQLPTIGASGSAIRQVSITRD
HCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHEECCCCCCCEECCCCCEEEEEEEEEC
PNNPYSTFQVGLGVTAYELDFWGRVRSLKDAALDNYLATQSTRDTTQISLVSQVAQAWLS
CCCCCCEEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
YSFAVANLKLADQTLKAQLDSYNLNKKRFDVGIDSEVPLRQAQISVETARNDVANYKTQI
HHHHEEEEHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHEEHHHHHHHHHHHHHHH
VQAQNLLNLLVGQSVPQNLLPNQPVKSITRQNVFSTGLPSDLLNNRPDVKSAEYQLSAAG
HHHHHHHHHHHCCCCCHHCCCCCHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCEEEEECC
ANIGAAKAALFPTISLTGSAGYASTQLSDLFKSGAGVWSIGPSLELPIFDWGTRRANIKI
CCCCHHHHHHEEEEEECCCCCCCHHHHHHHHHCCCCEEECCCCCCCEEEECCCCEEEEEE
SETDQKIALSNYEKSIQSAFREVNDALATRANIGDRISAQRRLVDATNTTYSLSNARFRA
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHEECCCCEEEECCCHHHC
GIDNYLTVLDAQRTSYSAEQGLLLLEQANLNNQIELYKTLGGGLKANTTDTVVHQPSSAD
CCHHHHEEEECHHCCCCHHCCEEEEEECCCCCCEEEEEHHCCCCCCCCCCCEEECCCCCC
LKKK
CCCC
>Mature Secondary Structure
MMQKVWSISGRSIAVSALALALAACQSMRGPEPVVKADIPTSYTYNASGQSIAEQGYKDF
CCCCHHCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCEEECCCCHHHHHHHHHHH
FSDPRLLQVIELALNNNRDLRTAALNIQRAQQQYQITENNQLPTIGASGSAIRQVSITRD
HCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHEECCCCCCCEECCCCCEEEEEEEEEC
PNNPYSTFQVGLGVTAYELDFWGRVRSLKDAALDNYLATQSTRDTTQISLVSQVAQAWLS
CCCCCCEEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
YSFAVANLKLADQTLKAQLDSYNLNKKRFDVGIDSEVPLRQAQISVETARNDVANYKTQI
HHHHEEEEHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHEEHHHHHHHHHHHHHHH
VQAQNLLNLLVGQSVPQNLLPNQPVKSITRQNVFSTGLPSDLLNNRPDVKSAEYQLSAAG
HHHHHHHHHHHCCCCCHHCCCCCHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCEEEEECC
ANIGAAKAALFPTISLTGSAGYASTQLSDLFKSGAGVWSIGPSLELPIFDWGTRRANIKI
CCCCHHHHHHEEEEEECCCCCCCHHHHHHHHHCCCCEEECCCCCCCEEEECCCCEEEEEE
SETDQKIALSNYEKSIQSAFREVNDALATRANIGDRISAQRRLVDATNTTYSLSNARFRA
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHEECCCCEEEECCCHHHC
GIDNYLTVLDAQRTSYSAEQGLLLLEQANLNNQIELYKTLGGGLKANTTDTVVHQPSSAD
CCHHHHEEEECHHCCCCHHCCEEEEEECCCCCCEEEEEHHCCCCCCCCCCCEEECCCCCC
LKKK
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]