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The map label for this gene is acrA [H]

Identifier: 50085970

GI number: 50085970

Start: 2872149

End: 2873414

Strand: Direct

Name: acrA [H]

Synonym: ACIAD2943

Alternate gene names: 50085970

Gene position: 2872149-2873414 (Clockwise)

Preceding gene: 50085969

Following gene: 50085971

Centisome position: 79.81

GC content: 44.47

Gene sequence:

>1266_bases
ATGACGTCGGCTAAGCTTTGGGTACCTGCCCTTACTGCTTGCGCATTGGCAACAAGTATTGCACTTGTTGGTTGTAGTAA
AGATCCTAAAAATGCTGAACAGGCTGGTGCTGCTCAGCAAATGCCACCACCACAGGTGGGTGTGATCGTTGCTCAACCGC
AAAGCGTAGAACAATCAGTAGAACTTTCTGGACGAACATCTGCTTACGAGATTTCTGAAGTTCGACCACAAACAAGCGGA
ATCATTTTAAAACGCTTGTTTGCAGAAGGTAGCTACGTTCGTGAAGGACAGGCGCTTTACGAACTAGATTCACGTACCAA
TCGTGCGACGCTCGACAATGCAAAGGCAGCACTTTTGCAACAACGGGCAAATCTAAACTCGCTTAAAACCAAGATGAATC
GTTATAAACAATTGGTTTCAAGTAATGCGGTTTCCAAACAGGAATACGATGATCTGGTCGGTCAGGTCAATGTAGCTGAG
GCACAAGTTGCAGCGGCACAGGCACAAGTGCAAAATGCGCAGGTTGATTTAGGTTACTCTACCATTCGCTCACCAATCTC
GGGTCAATCTGGTAAATCATCCGTAACTGCTGGTGCATTGGTTACAGCAAATCAGACCACACCACTGGTGACCATTCAAC
GAATGGATCCGATTTATGTAGATATCAATCAATCAAGTACAGAATTACTTCGCTTGCGTCAGCAATTGAGTAAAGGTAGT
CTGGACAACAGTAACAATACTAAGGTTAAACTTCGTCTGGAAGATGGTTCTATCTATCCAGTTGAAGGGCGCTTGGCATT
TTCTGATGCAAGTGTAAGCACTGATACAGGTACTGTTACCCTACGTGCTGTTTTCCCGAATCCTAATCATTTATTGCTTC
CTGGTATGTATGCAACTGCACAAATTGCTCAAGCTGTCATTCCAAATGCATATCTGATTCCTCAAGCTGCGGTTACACGT
ACACCAACGGGTCAAGCAACTGCAATGGTAGTCAATGCTAAAAATACCGCAGAAACACGTCAAATTCAGACTGCTGGTGT
ACAGGGTAAAGACTGGATTGTCACTGAAGGGCTTAAATCAGGTGATAAAGTGATTGTGGATGGTATTGCCAAGGTTAAAG
AAGGTCAGCAAGTTACTGCTCAACCTTACCAAGCACAACCACAACAGCAATCGCCTACTCAACCGCAAAATGCGGACAGC
GAAAAATCAAAGCCAGCGCAAGCAGATAAAGCCCAATCTGAACAAAAAGCTACTTCACATGCATAA

Upstream 100 bases:

>100_bases
TGGTTTTATTTGGATCTCATTGCTTTGGCTAGGGCTGTTAAAAATTAAGTTAGACAAAAACAATTTATTTTTTAGATAAT
TATCTAAATGAGGTGGAAGA

Downstream 100 bases:

>100_bases
GGAGTAGACTGAATGGCTAAATTTTTTATTCATCGCCCCATCTTTGCGTGGGTGATCGCACTGGTCATTATGCTGGCGGG
TATTCTTACTCTCACCAAAA

Product: acriflavine resistance protein A precursor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 421; Mature: 420

Protein sequence:

>421_residues
MTSAKLWVPALTACALATSIALVGCSKDPKNAEQAGAAQQMPPPQVGVIVAQPQSVEQSVELSGRTSAYEISEVRPQTSG
IILKRLFAEGSYVREGQALYELDSRTNRATLDNAKAALLQQRANLNSLKTKMNRYKQLVSSNAVSKQEYDDLVGQVNVAE
AQVAAAQAQVQNAQVDLGYSTIRSPISGQSGKSSVTAGALVTANQTTPLVTIQRMDPIYVDINQSSTELLRLRQQLSKGS
LDNSNNTKVKLRLEDGSIYPVEGRLAFSDASVSTDTGTVTLRAVFPNPNHLLLPGMYATAQIAQAVIPNAYLIPQAAVTR
TPTGQATAMVVNAKNTAETRQIQTAGVQGKDWIVTEGLKSGDKVIVDGIAKVKEGQQVTAQPYQAQPQQQSPTQPQNADS
EKSKPAQADKAQSEQKATSHA

Sequences:

>Translated_421_residues
MTSAKLWVPALTACALATSIALVGCSKDPKNAEQAGAAQQMPPPQVGVIVAQPQSVEQSVELSGRTSAYEISEVRPQTSG
IILKRLFAEGSYVREGQALYELDSRTNRATLDNAKAALLQQRANLNSLKTKMNRYKQLVSSNAVSKQEYDDLVGQVNVAE
AQVAAAQAQVQNAQVDLGYSTIRSPISGQSGKSSVTAGALVTANQTTPLVTIQRMDPIYVDINQSSTELLRLRQQLSKGS
LDNSNNTKVKLRLEDGSIYPVEGRLAFSDASVSTDTGTVTLRAVFPNPNHLLLPGMYATAQIAQAVIPNAYLIPQAAVTR
TPTGQATAMVVNAKNTAETRQIQTAGVQGKDWIVTEGLKSGDKVIVDGIAKVKEGQQVTAQPYQAQPQQQSPTQPQNADS
EKSKPAQADKAQSEQKATSHA
>Mature_420_residues
TSAKLWVPALTACALATSIALVGCSKDPKNAEQAGAAQQMPPPQVGVIVAQPQSVEQSVELSGRTSAYEISEVRPQTSGI
ILKRLFAEGSYVREGQALYELDSRTNRATLDNAKAALLQQRANLNSLKTKMNRYKQLVSSNAVSKQEYDDLVGQVNVAEA
QVAAAQAQVQNAQVDLGYSTIRSPISGQSGKSSVTAGALVTANQTTPLVTIQRMDPIYVDINQSSTELLRLRQQLSKGSL
DNSNNTKVKLRLEDGSIYPVEGRLAFSDASVSTDTGTVTLRAVFPNPNHLLLPGMYATAQIAQAVIPNAYLIPQAAVTRT
PTGQATAMVVNAKNTAETRQIQTAGVQGKDWIVTEGLKSGDKVIVDGIAKVKEGQQVTAQPYQAQPQQQSPTQPQNADSE
KSKPAQADKAQSEQKATSHA

Specific function: AcrAB is a drug efflux protein with a broad substrate specificity [H]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1786668, Length=403, Percent_Identity=47.1464019851117, Blast_Score=338, Evalue=3e-94,
Organism=Escherichia coli, GI1789665, Length=371, Percent_Identity=45.822102425876, Blast_Score=316, Evalue=2e-87,
Organism=Escherichia coli, GI1789929, Length=372, Percent_Identity=43.8172043010753, Blast_Score=306, Evalue=1e-84,
Organism=Escherichia coli, GI87082036, Length=322, Percent_Identity=28.2608695652174, Blast_Score=123, Evalue=3e-29,
Organism=Escherichia coli, GI87081796, Length=343, Percent_Identity=24.7813411078717, Blast_Score=73, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 44881; Mature: 44750

Theoretical pI: Translated: 9.34; Mature: 9.34

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSAKLWVPALTACALATSIALVGCSKDPKNAEQAGAAQQMPPPQVGVIVAQPQSVEQSV
CCCCCHHHHHHHHHHHHHHHHEEECCCCCCCHHHCCCCCCCCCCCEEEEEECCCCHHHHH
ELSGRTSAYEISEVRPQTSGIILKRLFAEGSYVREGQALYELDSRTNRATLDNAKAALLQ
HCCCCCCEEEHHHCCCCHHHHHHHHHHHCCCEEECCCHHHHHHCCCCCCHHHHHHHHHHH
QRANLNSLKTKMNRYKQLVSSNAVSKQEYDDLVGQVNVAEAQVAAAQAQVQNAQVDLGYS
HHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCEEEECHH
TIRSPISGQSGKSSVTAGALVTANQTTPLVTIQRMDPIYVDINQSSTELLRLRQQLSKGS
HHCCCCCCCCCCCCEEECEEEECCCCCCEEEEECCCCEEEEECCCHHHHHHHHHHHHCCC
LDNSNNTKVKLRLEDGSIYPVEGRLAFSDASVSTDTGTVTLRAVFPNPNHLLLPGMYATA
CCCCCCCEEEEEEECCCEEEECCEEEECCCCCCCCCCEEEEEEECCCCCEEEECCCHHHH
QIAQAVIPNAYLIPQAAVTRTPTGQATAMVVNAKNTAETRQIQTAGVQGKDWIVTEGLKS
HHHHHHCCCCEECCCHHHCCCCCCCEEEEEEECCCCCHHHHHHHCCCCCCCEEEECCCCC
GDKVIVDGIAKVKEGQQVTAQPYQAQPQQQSPTQPQNADSEKSKPAQADKAQSEQKATSH
CCEEEEHHHHHHCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCC
A
C
>Mature Secondary Structure 
TSAKLWVPALTACALATSIALVGCSKDPKNAEQAGAAQQMPPPQVGVIVAQPQSVEQSV
CCCCHHHHHHHHHHHHHHHHEEECCCCCCCHHHCCCCCCCCCCCEEEEEECCCCHHHHH
ELSGRTSAYEISEVRPQTSGIILKRLFAEGSYVREGQALYELDSRTNRATLDNAKAALLQ
HCCCCCCEEEHHHCCCCHHHHHHHHHHHCCCEEECCCHHHHHHCCCCCCHHHHHHHHHHH
QRANLNSLKTKMNRYKQLVSSNAVSKQEYDDLVGQVNVAEAQVAAAQAQVQNAQVDLGYS
HHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCEEEECHH
TIRSPISGQSGKSSVTAGALVTANQTTPLVTIQRMDPIYVDINQSSTELLRLRQQLSKGS
HHCCCCCCCCCCCCEEECEEEECCCCCCEEEEECCCCEEEEECCCHHHHHHHHHHHHCCC
LDNSNNTKVKLRLEDGSIYPVEGRLAFSDASVSTDTGTVTLRAVFPNPNHLLLPGMYATA
CCCCCCCEEEEEEECCCEEEECCEEEECCCCCCCCCCEEEEEEECCCCCEEEECCCHHHH
QIAQAVIPNAYLIPQAAVTRTPTGQATAMVVNAKNTAETRQIQTAGVQGKDWIVTEGLKS
HHHHHHCCCCEECCCHHHCCCCCCCEEEEEEECCCCCHHHHHHHCCCCCCCEEEECCCCC
GDKVIVDGIAKVKEGQQVTAQPYQAQPQQQSPTQPQNADSEKSKPAQADKAQSEQKATSH
CCEEEEHHHHHHCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCC
A
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]