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The map label for this gene is lolA [H]

Identifier: 50085964

GI number: 50085964

Start: 2866380

End: 2867072

Strand: Reverse

Name: lolA [H]

Synonym: ACIAD2937

Alternate gene names: 50085964

Gene position: 2867072-2866380 (Counterclockwise)

Preceding gene: 50085965

Following gene: 50085963

Centisome position: 79.67

GC content: 41.99

Gene sequence:

>693_bases
ATGACGATGCTTCGTAAAACCGTGTGTGCCATCGCAATGAGTGCAGCAACACTTGCACCTGTTATGTCAACGACAGTATT
TGCTGCTACCCCAGCATCGGAGCAACAAGCAACTTCAAATCTGGTAAAACAGCTCAGTGGGATTCAAAGTCTGAGTGCCA
GTTTTGAACAAACAACGAAAACGACTAATGCTAAAGCACCACAAAAGAAAGGTTTGTCTGCACAGCATATGAACCAAACC
TTTAAAGGTGAAATGAAGGTTGCACGACCAGGAAAGTTTTTCTGGGAAACCACTTCGCCTTCAAAGCAAACGATTGCCAC
GACAGGTAAAACCGTCTGGATTTATGATCCTGACTTACAACAAGCCGTTCGTCAAAATCTGGATGATCAGGTGGCCAATA
CACCTGCTTTATTATTATCTGGAAATACCAGTCAGATTATGCAGTCGTATCGCGTAACTCAGCCAGATCGTAGTAAAACG
TACTACACTTTATATCCTAAAAATAGTGAAGGGGCTTTCCAGAGCCTAACCATTAGTTTTGGTGCAAACAAAGCTCCAAG
CCTGATGATTTTGCAGGATTCTTTAGGTCAAACAACTTACGTGCGGTTTAACAATGTCAAACTAAATCCTGCTATTCCAG
CATCAACATTTAACTTCACGCCACCTAAAGGCACGGATATTATTGATCAATAA

Upstream 100 bases:

>100_bases
AAAACTACAAAATGTCTTATTTTTTCAGTTCTTAGCATATAATTTGTCGGTTAATATAAAAATCAAATAGATCGAATACA
GTGAAAGACAAGGTGATTCT

Downstream 100 bases:

>100_bases
TATCTTTAAGATGTCGAAAATATTATTTCAATTAATCGCTCATTTAGGGCGATTAATTTTTTATGGTGTCATTAAATAGA
TACATTTCTTCAATGTTCAG

Product: outer-membrane lipoproteins carrier protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 230; Mature: 229

Protein sequence:

>230_residues
MTMLRKTVCAIAMSAATLAPVMSTTVFAATPASEQQATSNLVKQLSGIQSLSASFEQTTKTTNAKAPQKKGLSAQHMNQT
FKGEMKVARPGKFFWETTSPSKQTIATTGKTVWIYDPDLQQAVRQNLDDQVANTPALLLSGNTSQIMQSYRVTQPDRSKT
YYTLYPKNSEGAFQSLTISFGANKAPSLMILQDSLGQTTYVRFNNVKLNPAIPASTFNFTPPKGTDIIDQ

Sequences:

>Translated_230_residues
MTMLRKTVCAIAMSAATLAPVMSTTVFAATPASEQQATSNLVKQLSGIQSLSASFEQTTKTTNAKAPQKKGLSAQHMNQT
FKGEMKVARPGKFFWETTSPSKQTIATTGKTVWIYDPDLQQAVRQNLDDQVANTPALLLSGNTSQIMQSYRVTQPDRSKT
YYTLYPKNSEGAFQSLTISFGANKAPSLMILQDSLGQTTYVRFNNVKLNPAIPASTFNFTPPKGTDIIDQ
>Mature_229_residues
TMLRKTVCAIAMSAATLAPVMSTTVFAATPASEQQATSNLVKQLSGIQSLSASFEQTTKTTNAKAPQKKGLSAQHMNQTF
KGEMKVARPGKFFWETTSPSKQTIATTGKTVWIYDPDLQQAVRQNLDDQVANTPALLLSGNTSQIMQSYRVTQPDRSKTY
YTLYPKNSEGAFQSLTISFGANKAPSLMILQDSLGQTTYVRFNNVKLNPAIPASTFNFTPPKGTDIIDQ

Specific function: Participates in the translocation of lipoproteins from the inner membrane to the outer membrane. Only forms a complex with a lipoprotein if the residue after the N-terminal Cys is not an aspartate (The Asp acts as a targeting signal to indicate that the l

COG id: COG2834

COG function: function code M; Outer membrane lipoprotein-sorting protein

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lolA family [H]

Homologues:

Organism=Escherichia coli, GI145693108, Length=220, Percent_Identity=29.0909090909091, Blast_Score=96, Evalue=3e-21,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004564
- InterPro:   IPR018323 [H]

Pfam domain/function: PF03548 LolA [H]

EC number: NA

Molecular weight: Translated: 25089; Mature: 24958

Theoretical pI: Translated: 10.25; Mature: 10.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMLRKTVCAIAMSAATLAPVMSTTVFAATPASEQQATSNLVKQLSGIQSLSASFEQTTK
CCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
TTNAKAPQKKGLSAQHMNQTFKGEMKVARPGKFFWETTSPSKQTIATTGKTVWIYDPDLQ
CCCCCCCHHHCCCHHHHCHHHCCCEEEECCCCEEEECCCCCCCEEEECCCEEEEECCCHH
QAVRQNLDDQVANTPALLLSGNTSQIMQSYRVTQPDRSKTYYTLYPKNSEGAFQSLTISF
HHHHHCCHHHHHCCCEEEEECCHHHHHHHHCCCCCCCCCEEEEEECCCCCCCEEEEEEEE
GANKAPSLMILQDSLGQTTYVRFNNVKLNPAIPASTFNFTPPKGTDIIDQ
CCCCCCEEEEEECCCCCEEEEEEECEEECCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TMLRKTVCAIAMSAATLAPVMSTTVFAATPASEQQATSNLVKQLSGIQSLSASFEQTTK
CHHHHHHHHHHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
TTNAKAPQKKGLSAQHMNQTFKGEMKVARPGKFFWETTSPSKQTIATTGKTVWIYDPDLQ
CCCCCCCHHHCCCHHHHCHHHCCCEEEECCCCEEEECCCCCCCEEEECCCEEEEECCCHH
QAVRQNLDDQVANTPALLLSGNTSQIMQSYRVTQPDRSKTYYTLYPKNSEGAFQSLTISF
HHHHHCCHHHHHCCCEEEEECCHHHHHHHHCCCCCCCCCEEEEEECCCCCCCEEEEEEEE
GANKAPSLMILQDSLGQTTYVRFNNVKLNPAIPASTFNFTPPKGTDIIDQ
CCCCCCEEEEEECCCCCEEEEEEECEEECCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA