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The map label for this gene is lipB

Identifier: 50085954

GI number: 50085954

Start: 2856887

End: 2857546

Strand: Direct

Name: lipB

Synonym: ACIAD2927

Alternate gene names: 50085954

Gene position: 2856887-2857546 (Clockwise)

Preceding gene: 50085953

Following gene: 50085955

Centisome position: 79.39

GC content: 40.91

Gene sequence:

>660_bases
GTGAATGATACTGTTCTCAAACCCAATCTCATCATTCGTCATTATAAAAATCTGCACAATTATGCATCGTTATTCCAAGA
AATGAGAAGCTATACAGATCAGCGGGATGAACATTCTCCAGATCAGCTTTGGATACTACAGCATCATCCTGTTCTCACTC
AGGGACAGGCAGGTAAACCTGAACATATTCTCATACCCAGTGACCTTCCCGTGGTTCAGTCAGATCGTGGCGGTCAAGTC
ACTTGGCATGGCCCTGGTCAAATGGTGATTTATTTTATGTTTGATTTAAATCGCCTGAAATGGAATGTACGTACACTGGT
GTCATTTGCCGAACAGCTGATGATTGATTTGATTAAAAAATACAATATAGACGCTTATGCCAAAGCCGATGCACCTGGCG
TTTACGTAGCAGAACGCAAAATTGGCTCTTTAGGTTTTAAAATCCGCCGTGGCCGAAGCTATCATGGTCTGGCGCTGAAT
ATCGATTGTGATTTATCTGGCTTTCAAACCATTAATCCATGTGGTTACGCAGGATTAGAAATGGTCAGAATCTGTGATCT
TGTCGAAAATTATCCTCGATTTGATCAGCTTGCTCAGGATGTTACAGCTTACTTCAAAGAAAAAAGTGACTTTAATGACG
TTCAAGTCATCTTGCAATAA

Upstream 100 bases:

>100_bases
GCTTGCCCACATATTCGTACTGCCCTCTAAGATAAAGACACGCCTTCTGGCGTGTTTTTTATTGGCATTTCTTTTTGCTT
TTTAATCAAGGACACGATCT

Downstream 100 bases:

>100_bases
ATGACTTTCAGTTCGAGAAAAAATCAATTAGAGTAATTACTCTAAATAAAATTAGAATCTGTAAAAAGGGATAAGCACGT
GATTTCAACCAAAGCCATGA

Product: lipoate-protein ligase B

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acylcarrier-protein]-protein N-octanoyltransferase

Number of amino acids: Translated: 219; Mature: 219

Protein sequence:

>219_residues
MNDTVLKPNLIIRHYKNLHNYASLFQEMRSYTDQRDEHSPDQLWILQHHPVLTQGQAGKPEHILIPSDLPVVQSDRGGQV
TWHGPGQMVIYFMFDLNRLKWNVRTLVSFAEQLMIDLIKKYNIDAYAKADAPGVYVAERKIGSLGFKIRRGRSYHGLALN
IDCDLSGFQTINPCGYAGLEMVRICDLVENYPRFDQLAQDVTAYFKEKSDFNDVQVILQ

Sequences:

>Translated_219_residues
MNDTVLKPNLIIRHYKNLHNYASLFQEMRSYTDQRDEHSPDQLWILQHHPVLTQGQAGKPEHILIPSDLPVVQSDRGGQV
TWHGPGQMVIYFMFDLNRLKWNVRTLVSFAEQLMIDLIKKYNIDAYAKADAPGVYVAERKIGSLGFKIRRGRSYHGLALN
IDCDLSGFQTINPCGYAGLEMVRICDLVENYPRFDQLAQDVTAYFKEKSDFNDVQVILQ
>Mature_219_residues
MNDTVLKPNLIIRHYKNLHNYASLFQEMRSYTDQRDEHSPDQLWILQHHPVLTQGQAGKPEHILIPSDLPVVQSDRGGQV
TWHGPGQMVIYFMFDLNRLKWNVRTLVSFAEQLMIDLIKKYNIDAYAKADAPGVYVAERKIGSLGFKIRRGRSYHGLALN
IDCDLSGFQTINPCGYAGLEMVRICDLVENYPRFDQLAQDVTAYFKEKSDFNDVQVILQ

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family

Homologues:

Organism=Homo sapiens, GI221554520, Length=136, Percent_Identity=32.3529411764706, Blast_Score=64, Evalue=8e-11,
Organism=Escherichia coli, GI87081767, Length=167, Percent_Identity=56.8862275449102, Blast_Score=215, Evalue=2e-57,
Organism=Saccharomyces cerevisiae, GI6323268, Length=183, Percent_Identity=32.7868852459016, Blast_Score=87, Evalue=2e-18,
Organism=Drosophila melanogaster, GI21355483, Length=106, Percent_Identity=33.0188679245283, Blast_Score=75, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPB_ACIAD (Q6F8H3)

Other databases:

- EMBL:   CR543861
- RefSeq:   YP_047464.1
- ProteinModelPortal:   Q6F8H3
- SMR:   Q6F8H3
- STRING:   Q6F8H3
- GeneID:   2881111
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD2927
- NMPDR:   fig|62977.3.peg.2657
- eggNOG:   COG0321
- HOGENOM:   HBG716160
- OMA:   RDELISC
- PhylomeDB:   Q6F8H3
- ProtClustDB:   PRK14342
- BioCyc:   ASP62977:ACIAD2927-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00013
- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605
- PIRSF:   PIRSF016262
- TIGRFAMs:   TIGR00214

Pfam domain/function: PF03099 BPL_LipA_LipB

EC number: =2.3.1.181

Molecular weight: Translated: 25216; Mature: 25216

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: PS01313 LIPB

Important sites: ACT_SITE 174-174

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDTVLKPNLIIRHYKNLHNYASLFQEMRSYTDQRDEHSPDQLWILQHHPVLTQGQAGKP
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCEEECCCCCCC
EHILIPSDLPVVQSDRGGQVTWHGPGQMVIYFMFDLNRLKWNVRTLVSFAEQLMIDLIKK
CEEECCCCCCEEECCCCCEEEEECCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHH
YNIDAYAKADAPGVYVAERKIGSLGFKIRRGRSYHGLALNIDCDLSGFQTINPCGYAGLE
CCCCEEECCCCCCEEEEHHHHCCCCEEEECCCCCCCEEEEEECCCCCCCCCCCCCHHHHH
MVRICDLVENYPRFDQLAQDVTAYFKEKSDFNDVQVILQ
HHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCEEEEEC
>Mature Secondary Structure
MNDTVLKPNLIIRHYKNLHNYASLFQEMRSYTDQRDEHSPDQLWILQHHPVLTQGQAGKP
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCEEECCCCCCC
EHILIPSDLPVVQSDRGGQVTWHGPGQMVIYFMFDLNRLKWNVRTLVSFAEQLMIDLIKK
CEEECCCCCCEEECCCCCEEEEECCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHH
YNIDAYAKADAPGVYVAERKIGSLGFKIRRGRSYHGLALNIDCDLSGFQTINPCGYAGLE
CCCCEEECCCCCCEEEEHHHHCCCCEEEECCCCCCCEEEEEECCCCCCCCCCCCCHHHHH
MVRICDLVENYPRFDQLAQDVTAYFKEKSDFNDVQVILQ
HHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA