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The map label for this gene is dnaG [H]

Identifier: 50085933

GI number: 50085933

Start: 2837360

End: 2839249

Strand: Direct

Name: dnaG [H]

Synonym: ACIAD2899

Alternate gene names: 50085933

Gene position: 2837360-2839249 (Clockwise)

Preceding gene: 50085932

Following gene: 50085935

Centisome position: 78.85

GC content: 41.85

Gene sequence:

>1890_bases
ATGGCTATTCCTCAGCATACCATTGATCAAATTCTAGATCGGACTGATCTGGTTGAGTTAATAGGCCAGAGAGTCAAACT
AAAAAAAACAGGTAGAACCTATTCGGGTTGCTGTCCATTTCATCAAGAAAAAAGCCCTTCTTTCCATGTATATCGAGACA
AGGGCTATTACCATTGCTTTGGCTGTCAGGCCAACGGTAATGCCATCCGTTTTTTGATGGATATTGATGGGCGTAACTTT
ATTGATGTCATGCAGGAACTGTCTAGTAAATCGGGCGTTGAACTTCCTAAAGATAACTTTGAACAAAAGAAACTTTCCTA
TAAGCGCAATACACAAAAACCAGAACCGAAACCTGTTGTAAATACTGAAAAATCTGCACCATCTCACCATGCTGCAATAT
CCGAATCATCGGAACAGATTGACGGTTTACAAGCACCCTCTTACTTTGACGATCCATTCGCACAGTTTGATCAAAGCTAT
ATGGGTTTTGAAGATGCCCCTCAAGAAGGCAATCTTTATGACCTATTGGAAAATGTAGCTCAGTTTTATGAAAAACAGCT
GCCAAATAGTAATAAGGCACAGCAATATTTTAAACAACGTGGCTTGAGTGCAGAAACTATTCAATTCTGGCGTTTGGGTT
ATGCACCCGAAGACTGGCAGCACCTTGAAAAAGCCTTTCCTTATGATATCGAAGGATTAAAGCAAGTGGGACTCATCCGC
TCAAGTGATAGTGGACGTGACTTTGACCTGCTACGTGAGCGTGTCATCTTCCCGATTCGTGATCATAAAGGGCGTGTGGT
CGGTTTTGGCGGTCGTGCGCTGAATGATGAAATCAAGCCTAAATATATCAACTCACCAGACTCCGAGATTTTCCATAAAA
ACCAGTTACTTTATGGTCTATATGAAGGCAGAAAACTCAAGGCAAAAGACTGGTTAATGGTAGAAGGCTATATGGACGTG
ATTGCTTTACAGCAATATGGCGTCTATGGTGCGGTAGCAACCTTAGGCACAGCCAGTAATGCTGATCACTTAAATACATT
ATTTAAACAGAACTCTAGAATCACGATTGCCTTTGATGGCGATGCAGCAGGGCAAAAAGCGGCACGTCGAACCTTGGAAA
TTGCCCTTCCCTCACTCAACGATGGTCGAGAACTCAAGTTTTTTGTACTTCCCAAAGAGCATGACCCAGACTCGCTCATT
CGCCGTGAAGGATTGGAACATTTTCAGCGCTTACTTGAGCAAGCACCCTTACTTTCCGATTTTATTTTTGCACATTTAAC
ACAGCAACATGACATCTCTACGCCTGAGGGCAAAAGTCAGGTCATGGGCGAGCTGCGTGAACTCACAGAGTTATTACCCA
AACAAGGCTCTTTTCGCTACCTGCTCACACAATCTTTCCGTGAAAAGCTCGGTTTGGGGCGTAAATGGACGCCTCAAATC
AGTCATGATGCCTCGTTATCCTTTAATATCCAGACCAAAGATGAAGATTTTGCTGTAGCATTATTGATGCAGCATCCTTA
TCTTTATATTCACTTCGAGGCTTTGCGGGCTTTTATTGATCCACAACAATTACTGGCTCAAATATTAGCCATCATTGACC
GTGTCTTTGATGAATTACCCGATGATCAGGAACTGGCCACTTATTACGTTTTAGGCGCCTGCAATCAGTTTAGTCATGAA
ATTGCAGATATCATTGAACGTACCGACATTCAAACCTTAATGAATGCACCAGAAACTGCCGATAAACATGCCAAAGAATA
TGCGCTGGGCTTGAAAGAAAAATATTTGCGTCAAAAGTTAAAGGCACCTATTTCATTGATTGAATCACGCAATCTACGTC
AACAGCTCAATGAATTAAGCAAACAGATCAACTTAAGATTATTATCTTGA

Upstream 100 bases:

>100_bases
TATTTTTGCTTACGATCAAAATTCTTTTTTGGCTTTTCATCATATACACTAATAAATTGTTATTTTAAAAACTGCGCTTG
TCGTTAGGATATTTCTCGTT

Downstream 100 bases:

>100_bases
ATTATCGATCCAGATTGTTTTTAGGCTTGCGCTGATGCTCAAATACGTCTGTCAGTTGTTCTTTCATCCACTCAAAATAA
TCTGGACTTTCATGCTGTGC

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 629; Mature: 628

Protein sequence:

>629_residues
MAIPQHTIDQILDRTDLVELIGQRVKLKKTGRTYSGCCPFHQEKSPSFHVYRDKGYYHCFGCQANGNAIRFLMDIDGRNF
IDVMQELSSKSGVELPKDNFEQKKLSYKRNTQKPEPKPVVNTEKSAPSHHAAISESSEQIDGLQAPSYFDDPFAQFDQSY
MGFEDAPQEGNLYDLLENVAQFYEKQLPNSNKAQQYFKQRGLSAETIQFWRLGYAPEDWQHLEKAFPYDIEGLKQVGLIR
SSDSGRDFDLLRERVIFPIRDHKGRVVGFGGRALNDEIKPKYINSPDSEIFHKNQLLYGLYEGRKLKAKDWLMVEGYMDV
IALQQYGVYGAVATLGTASNADHLNTLFKQNSRITIAFDGDAAGQKAARRTLEIALPSLNDGRELKFFVLPKEHDPDSLI
RREGLEHFQRLLEQAPLLSDFIFAHLTQQHDISTPEGKSQVMGELRELTELLPKQGSFRYLLTQSFREKLGLGRKWTPQI
SHDASLSFNIQTKDEDFAVALLMQHPYLYIHFEALRAFIDPQQLLAQILAIIDRVFDELPDDQELATYYVLGACNQFSHE
IADIIERTDIQTLMNAPETADKHAKEYALGLKEKYLRQKLKAPISLIESRNLRQQLNELSKQINLRLLS

Sequences:

>Translated_629_residues
MAIPQHTIDQILDRTDLVELIGQRVKLKKTGRTYSGCCPFHQEKSPSFHVYRDKGYYHCFGCQANGNAIRFLMDIDGRNF
IDVMQELSSKSGVELPKDNFEQKKLSYKRNTQKPEPKPVVNTEKSAPSHHAAISESSEQIDGLQAPSYFDDPFAQFDQSY
MGFEDAPQEGNLYDLLENVAQFYEKQLPNSNKAQQYFKQRGLSAETIQFWRLGYAPEDWQHLEKAFPYDIEGLKQVGLIR
SSDSGRDFDLLRERVIFPIRDHKGRVVGFGGRALNDEIKPKYINSPDSEIFHKNQLLYGLYEGRKLKAKDWLMVEGYMDV
IALQQYGVYGAVATLGTASNADHLNTLFKQNSRITIAFDGDAAGQKAARRTLEIALPSLNDGRELKFFVLPKEHDPDSLI
RREGLEHFQRLLEQAPLLSDFIFAHLTQQHDISTPEGKSQVMGELRELTELLPKQGSFRYLLTQSFREKLGLGRKWTPQI
SHDASLSFNIQTKDEDFAVALLMQHPYLYIHFEALRAFIDPQQLLAQILAIIDRVFDELPDDQELATYYVLGACNQFSHE
IADIIERTDIQTLMNAPETADKHAKEYALGLKEKYLRQKLKAPISLIESRNLRQQLNELSKQINLRLLS
>Mature_628_residues
AIPQHTIDQILDRTDLVELIGQRVKLKKTGRTYSGCCPFHQEKSPSFHVYRDKGYYHCFGCQANGNAIRFLMDIDGRNFI
DVMQELSSKSGVELPKDNFEQKKLSYKRNTQKPEPKPVVNTEKSAPSHHAAISESSEQIDGLQAPSYFDDPFAQFDQSYM
GFEDAPQEGNLYDLLENVAQFYEKQLPNSNKAQQYFKQRGLSAETIQFWRLGYAPEDWQHLEKAFPYDIEGLKQVGLIRS
SDSGRDFDLLRERVIFPIRDHKGRVVGFGGRALNDEIKPKYINSPDSEIFHKNQLLYGLYEGRKLKAKDWLMVEGYMDVI
ALQQYGVYGAVATLGTASNADHLNTLFKQNSRITIAFDGDAAGQKAARRTLEIALPSLNDGRELKFFVLPKEHDPDSLIR
REGLEHFQRLLEQAPLLSDFIFAHLTQQHDISTPEGKSQVMGELRELTELLPKQGSFRYLLTQSFREKLGLGRKWTPQIS
HDASLSFNIQTKDEDFAVALLMQHPYLYIHFEALRAFIDPQQLLAQILAIIDRVFDELPDDQELATYYVLGACNQFSHEI
ADIIERTDIQTLMNAPETADKHAKEYALGLKEKYLRQKLKAPISLIESRNLRQQLNELSKQINLRLLS

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=477, Percent_Identity=39.832285115304, Blast_Score=333, Evalue=1e-92,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016136
- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR013173
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF08278 DnaG_DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 72103; Mature: 71971

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIPQHTIDQILDRTDLVELIGQRVKLKKTGRTYSGCCPFHQEKSPSFHVYRDKGYYHCF
CCCCHHHHHHHHCHHHHHHHHHHHEEEHHCCCCCCCCCCCCCCCCCCEEEEECCCEEEEE
GCQANGNAIRFLMDIDGRNFIDVMQELSSKSGVELPKDNFEQKKLSYKRNTQKPEPKPVV
EEECCCCEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCC
NTEKSAPSHHAAISESSEQIDGLQAPSYFDDPFAQFDQSYMGFEDAPQEGNLYDLLENVA
CCCCCCCCHHHHHCCCHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
QFYEKQLPNSNKAQQYFKQRGLSAETIQFWRLGYAPEDWQHLEKAFPYDIEGLKQVGLIR
HHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHCCEE
SSDSGRDFDLLRERVIFPIRDHKGRVVGFGGRALNDEIKPKYINSPDSEIFHKNQLLYGL
CCCCCCCHHHHHHHHEEEEECCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHCCEEEEE
YEGRKLKAKDWLMVEGYMDVIALQQYGVYGAVATLGTASNADHLNTLFKQNSRITIAFDG
CCCCEECCCCEEEEHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHCCCEEEEEECC
DAAGQKAARRTLEIALPSLNDGRELKFFVLPKEHDPDSLIRREGLEHFQRLLEQAPLLSD
CCCCHHHHHHHHEEECCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCHHHH
FIFAHLTQQHDISTPEGKSQVMGELRELTELLPKQGSFRYLLTQSFREKLGLGRKWTPQI
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCC
SHDASLSFNIQTKDEDFAVALLMQHPYLYIHFEALRAFIDPQQLLAQILAIIDRVFDELP
CCCCCEEEEEEECCCCCEEEHHHHCCEEEEEHHHHHHHCCHHHHHHHHHHHHHHHHHHCC
DDQELATYYVLGACNQFSHEIADIIERTDIQTLMNAPETADKHAKEYALGLKEKYLRQKL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
KAPISLIESRNLRQQLNELSKQINLRLLS
HHHHHHHHHHHHHHHHHHHHHHHCCEECC
>Mature Secondary Structure 
AIPQHTIDQILDRTDLVELIGQRVKLKKTGRTYSGCCPFHQEKSPSFHVYRDKGYYHCF
CCCHHHHHHHHCHHHHHHHHHHHEEEHHCCCCCCCCCCCCCCCCCCEEEEECCCEEEEE
GCQANGNAIRFLMDIDGRNFIDVMQELSSKSGVELPKDNFEQKKLSYKRNTQKPEPKPVV
EEECCCCEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCC
NTEKSAPSHHAAISESSEQIDGLQAPSYFDDPFAQFDQSYMGFEDAPQEGNLYDLLENVA
CCCCCCCCHHHHHCCCHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
QFYEKQLPNSNKAQQYFKQRGLSAETIQFWRLGYAPEDWQHLEKAFPYDIEGLKQVGLIR
HHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHCCEE
SSDSGRDFDLLRERVIFPIRDHKGRVVGFGGRALNDEIKPKYINSPDSEIFHKNQLLYGL
CCCCCCCHHHHHHHHEEEEECCCCCEEEECCCCCCCCCCCCCCCCCCHHHHHHCCEEEEE
YEGRKLKAKDWLMVEGYMDVIALQQYGVYGAVATLGTASNADHLNTLFKQNSRITIAFDG
CCCCEECCCCEEEEHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHCCCEEEEEECC
DAAGQKAARRTLEIALPSLNDGRELKFFVLPKEHDPDSLIRREGLEHFQRLLEQAPLLSD
CCCCHHHHHHHHEEECCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCHHHH
FIFAHLTQQHDISTPEGKSQVMGELRELTELLPKQGSFRYLLTQSFREKLGLGRKWTPQI
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCC
SHDASLSFNIQTKDEDFAVALLMQHPYLYIHFEALRAFIDPQQLLAQILAIIDRVFDELP
CCCCCEEEEEEECCCCCEEEHHHHCCEEEEEHHHHHHHCCHHHHHHHHHHHHHHHHHHCC
DDQELATYYVLGACNQFSHEIADIIERTDIQTLMNAPETADKHAKEYALGLKEKYLRQKL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
KAPISLIESRNLRQQLNELSKQINLRLLS
HHHHHHHHHHHHHHHHHHHHHHHCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8294018; 7542800; 8316085 [H]