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The map label for this gene is folD

Identifier: 50085890

GI number: 50085890

Start: 2785081

End: 2785929

Strand: Reverse

Name: folD

Synonym: ACIAD2847

Alternate gene names: 50085890

Gene position: 2785929-2785081 (Counterclockwise)

Preceding gene: 50085891

Following gene: 50085889

Centisome position: 77.42

GC content: 45.7

Gene sequence:

>849_bases
GTGGCATTGGTTTTAGATGGTCGTGCATTAGCGAAAAAAATTGAAGCTGATTTATTGGTGCGTGTAGAAGCACTTAAAGC
AAAGTCAGGTCGTACCCCAATTTTAGCCACAATTTTAGTTGGAGATGATGGTGCATCTGCAACTTATGTGCGTATGAAAG
GTAATGCCTGCCGTCGAGTGGGAATGGATTCATTAAAAATCGAACTCTCTCAGGAAACCACGACAGAGCAGTTACTTGCA
GAAATCGAGAAGCTCAATGCCAATCCAGATGTTCATGGTATTTTGCTTCAACATCCTGTTCCAGCTCAAATTGATGAGCG
TGCGTGCTTTGATGCGATTTCTTTAGCCAAAGATGTCGATGGTGTGACTTGTTTAGGTTATGGTCGTATGGCAATGGGAG
AAGCAGCGTACGGTTCTGCGACGCCTGCGGGAATCATGACTATTCTAAAAGAAAACAACATTGAGATTGCTGGTAAGCAT
GCTGTAGTGGTAGGCCGTTCTGCAATTTTAGGTAAGCCAATGGCCGCCATGTTGCTTGAGGCCAATGCAACTGTAACCAT
TTGCCATTCACGTACTCAAAATCTAGCCGAATTTGTTAAACAAGCAGATATTATCGTGGGTGCTGTTGGTAAGGCAGAGT
TGATTCAAAAAGACTGGATTAAACCAGGTGCTGTGGTGGTCGATGCGGGCTTCCACCCTCGTGATGGCGGTGGGGTTGGT
GATATCCAGTTACAGGGGATTGAAGACGTTGCCTCTGCTTATACACCTGTACCAGGTGGTGTCGGACCAATGACCATTAC
GACATTGATCCGTCAAACGGTTGAGGCTGCAGAAAAAGCGCTGGCTTAA

Upstream 100 bases:

>100_bases
TTTGCGAATATGCACAGAAAAACAACATCTAAAATAAAATTGACCCAATTATCATGTATCATTTGCAAAAATTGATTCAT
ATCTACTTTGGAGATGTGCT

Downstream 100 bases:

>100_bases
TTTTTGATTGAAATGCTCAGAAGACGTAGTAGGGTTTTGTTACTAGGATCTGTGATCTAAGAAGTTGGTTGCTTTTGTTT
TGGCAAAAGTAACCAAAATT

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase

Number of amino acids: Translated: 282; Mature: 281

Protein sequence:

>282_residues
MALVLDGRALAKKIEADLLVRVEALKAKSGRTPILATILVGDDGASATYVRMKGNACRRVGMDSLKIELSQETTTEQLLA
EIEKLNANPDVHGILLQHPVPAQIDERACFDAISLAKDVDGVTCLGYGRMAMGEAAYGSATPAGIMTILKENNIEIAGKH
AVVVGRSAILGKPMAAMLLEANATVTICHSRTQNLAEFVKQADIIVGAVGKAELIQKDWIKPGAVVVDAGFHPRDGGGVG
DIQLQGIEDVASAYTPVPGGVGPMTITTLIRQTVEAAEKALA

Sequences:

>Translated_282_residues
MALVLDGRALAKKIEADLLVRVEALKAKSGRTPILATILVGDDGASATYVRMKGNACRRVGMDSLKIELSQETTTEQLLA
EIEKLNANPDVHGILLQHPVPAQIDERACFDAISLAKDVDGVTCLGYGRMAMGEAAYGSATPAGIMTILKENNIEIAGKH
AVVVGRSAILGKPMAAMLLEANATVTICHSRTQNLAEFVKQADIIVGAVGKAELIQKDWIKPGAVVVDAGFHPRDGGGVG
DIQLQGIEDVASAYTPVPGGVGPMTITTLIRQTVEAAEKALA
>Mature_281_residues
ALVLDGRALAKKIEADLLVRVEALKAKSGRTPILATILVGDDGASATYVRMKGNACRRVGMDSLKIELSQETTTEQLLAE
IEKLNANPDVHGILLQHPVPAQIDERACFDAISLAKDVDGVTCLGYGRMAMGEAAYGSATPAGIMTILKENNIEIAGKHA
VVVGRSAILGKPMAAMLLEANATVTICHSRTQNLAEFVKQADIIVGAVGKAELIQKDWIKPGAVVVDAGFHPRDGGGVGD
IQLQGIEDVASAYTPVPGGVGPMTITTLIRQTVEAAEKALA

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family

Homologues:

Organism=Homo sapiens, GI222136639, Length=292, Percent_Identity=40.4109589041096, Blast_Score=210, Evalue=1e-54,
Organism=Homo sapiens, GI222418558, Length=297, Percent_Identity=38.3838383838384, Blast_Score=177, Evalue=9e-45,
Organism=Homo sapiens, GI94721354, Length=300, Percent_Identity=39.6666666666667, Blast_Score=172, Evalue=2e-43,
Organism=Homo sapiens, GI36796743, Length=210, Percent_Identity=27.1428571428571, Blast_Score=81, Evalue=1e-15,
Organism=Escherichia coli, GI1786741, Length=277, Percent_Identity=41.5162454873646, Blast_Score=204, Evalue=4e-54,
Organism=Caenorhabditis elegans, GI17568735, Length=291, Percent_Identity=36.0824742268041, Blast_Score=175, Evalue=3e-44,
Organism=Saccharomyces cerevisiae, GI6319558, Length=288, Percent_Identity=39.9305555555556, Blast_Score=211, Evalue=8e-56,
Organism=Saccharomyces cerevisiae, GI6321643, Length=296, Percent_Identity=36.4864864864865, Blast_Score=189, Evalue=4e-49,
Organism=Drosophila melanogaster, GI17136816, Length=298, Percent_Identity=43.2885906040268, Blast_Score=211, Evalue=6e-55,
Organism=Drosophila melanogaster, GI17136818, Length=298, Percent_Identity=43.2885906040268, Blast_Score=211, Evalue=6e-55,
Organism=Drosophila melanogaster, GI24645718, Length=291, Percent_Identity=40.893470790378, Blast_Score=208, Evalue=3e-54,
Organism=Drosophila melanogaster, GI17137370, Length=291, Percent_Identity=40.893470790378, Blast_Score=208, Evalue=3e-54,
Organism=Drosophila melanogaster, GI62472483, Length=291, Percent_Identity=40.893470790378, Blast_Score=208, Evalue=3e-54,
Organism=Drosophila melanogaster, GI45551871, Length=291, Percent_Identity=40.893470790378, Blast_Score=208, Evalue=3e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOLD2_ACIAD (Q6F8N7)

Other databases:

- EMBL:   CR543861
- RefSeq:   YP_047400.1
- HSSP:   P11586
- ProteinModelPortal:   Q6F8N7
- SMR:   Q6F8N7
- STRING:   Q6F8N7
- GeneID:   2878592
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD2847
- NMPDR:   fig|62977.3.peg.2593
- eggNOG:   COG0190
- HOGENOM:   HBG328751
- OMA:   GPMTINT
- ProtClustDB:   PRK14192
- BioCyc:   ASP62977:ACIAD2847-MONOMER
- GO:   GO:0005488
- HAMAP:   MF_01576
- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR00085

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C

EC number: =1.5.1.5; =3.5.4.9

Molecular weight: Translated: 29541; Mature: 29409

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: PS00766 THF_DHG_CYH_1; PS00767 THF_DHG_CYH_2; PS00136 SUBTILASE_ASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALVLDGRALAKKIEADLLVRVEALKAKSGRTPILATILVGDDGASATYVRMKGNACRRV
CEEEECCHHHHHHHHHHHHEEEEEHHCCCCCCCEEEEEEEECCCCCEEEEEECCCHHHHC
GMDSLKIELSQETTTEQLLAEIEKLNANPDVHGILLQHPVPAQIDERACFDAISLAKDVD
CCCEEEEEECCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCC
GVTCLGYGRMAMGEAAYGSATPAGIMTILKENNIEIAGKHAVVVGRSAILGKPMAAMLLE
CEEEECCCCHHCCCCCCCCCCCHHHHEEEECCCEEEECCEEEEEECHHHHCCCHHHHEEE
ANATVTICHSRTQNLAEFVKQADIIVGAVGKAELIQKDWIKPGAVVVDAGFHPRDGGGVG
CCCEEEEECCCHHHHHHHHHHCCEEEECCCHHHHHHHHCCCCCCEEEECCCCCCCCCCCC
DIQLQGIEDVASAYTPVPGGVGPMTITTLIRQTVEAAEKALA
CEEECCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ALVLDGRALAKKIEADLLVRVEALKAKSGRTPILATILVGDDGASATYVRMKGNACRRV
EEEECCHHHHHHHHHHHHEEEEEHHCCCCCCCEEEEEEEECCCCCEEEEEECCCHHHHC
GMDSLKIELSQETTTEQLLAEIEKLNANPDVHGILLQHPVPAQIDERACFDAISLAKDVD
CCCEEEEEECCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCC
GVTCLGYGRMAMGEAAYGSATPAGIMTILKENNIEIAGKHAVVVGRSAILGKPMAAMLLE
CEEEECCCCHHCCCCCCCCCCCHHHHEEEECCCEEEECCEEEEEECHHHHCCCHHHHEEE
ANATVTICHSRTQNLAEFVKQADIIVGAVGKAELIQKDWIKPGAVVVDAGFHPRDGGGVG
CCCEEEEECCCHHHHHHHHHHCCEEEECCCHHHHHHHHCCCCCCEEEECCCCCCCCCCCC
DIQLQGIEDVASAYTPVPGGVGPMTITTLIRQTVEAAEKALA
CEEECCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA