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The map label for this gene is ttcA

Identifier: 50085874

GI number: 50085874

Start: 2765736

End: 2766641

Strand: Reverse

Name: ttcA

Synonym: ACIAD2829

Alternate gene names: 50085874

Gene position: 2766641-2765736 (Counterclockwise)

Preceding gene: 50085877

Following gene: 50085873

Centisome position: 76.88

GC content: 40.62

Gene sequence:

>906_bases
ATGTACTCGCCAGTTGAGTCCGAACAGGGATTTAATTTTAAGCCAGAGCTTCCTACAAGCTCAGCCTATTATCGCTTATT
GAAAAAGCTTCGTCGTCAAGTGGGTCACGCAATTCGTGATTTCAAAATGATTGAAGACGGAGACAAGGTAATGGTCTGCG
TCTCTGGTGGTAAGGACAGCTACACCTTGCTCGATATTTTGTTGCAGTTTAAACGCATTGCACCGATCAATTTCGATATT
GTTGCGGTTAACCTTGACCAGAAACAACCTGGTTTTCCAGAAGATGTTTTACCACGTTATATGGAAGAGAATAATATTCC
TTACTATATCCTGGAAAAAGACACCTATACCATTACCAAGCGTTTAACACCTGAAGGCAAAACCTATTGTGCGGTGTGTT
CTCGTTTGCGTCGTGGCTCGCTTTATGGTTTTGCACAAGAGATTGGTGCAACCAAAGTAGCATTGGGTCATCATCGTGAT
GATATTATTGCCACGTTCTTCCTGAATTTGTTCCATGGTGGTAGTTTAAAGGCAATGCCGCCAAAATTATTATCTTCGGA
TAAAAAGAATATATTGATTCGTCCTTTGGCATATGTCGAAGAAAAAGACATTATCAAGTATGCAGATTTACGTAAATTCC
CAATCATTCCTTGTAACTTATGTGGTTCACAGGAAAATCTGCAACGTGCCATGCTCAATGAGATGCTGCGTGAATGGGAT
AAACAGTATCCAAAGCGTTTGCATAGCATATTCGGTGCCTTGCAAAATGTTTCGCCGTCTCAACTGGCAGATCGTGATCT
ATTCGATTTTGAAATACTTGATTCCCAGCGTGAACTTGATTTTAAAGATCCCGAAGAAATGAAAAAACGCCTAGACATCG
TAAACTTAAGCTTTGCTGCTGACTAA

Upstream 100 bases:

>100_bases
TAAATATGACAATCATAAGAATTGCATAATCTTTAGGTGCGATTCACGTGTGAATCATATAAAATCGTGCGGTATTTTTA
TTTATGGGTCAGGTTTATCT

Downstream 100 bases:

>100_bases
TCAGTCAAAGCGCTAACTTATTTATAAGTTGGCGCTTTTTTTATACGAGTGATATTAAAAAAAAACAAAAAATGAATGAC
AGGTACAAAAAACGTTAACT

Product: C32 tRNA thiolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MYSPVESEQGFNFKPELPTSSAYYRLLKKLRRQVGHAIRDFKMIEDGDKVMVCVSGGKDSYTLLDILLQFKRIAPINFDI
VAVNLDQKQPGFPEDVLPRYMEENNIPYYILEKDTYTITKRLTPEGKTYCAVCSRLRRGSLYGFAQEIGATKVALGHHRD
DIIATFFLNLFHGGSLKAMPPKLLSSDKKNILIRPLAYVEEKDIIKYADLRKFPIIPCNLCGSQENLQRAMLNEMLREWD
KQYPKRLHSIFGALQNVSPSQLADRDLFDFEILDSQRELDFKDPEEMKKRLDIVNLSFAAD

Sequences:

>Translated_301_residues
MYSPVESEQGFNFKPELPTSSAYYRLLKKLRRQVGHAIRDFKMIEDGDKVMVCVSGGKDSYTLLDILLQFKRIAPINFDI
VAVNLDQKQPGFPEDVLPRYMEENNIPYYILEKDTYTITKRLTPEGKTYCAVCSRLRRGSLYGFAQEIGATKVALGHHRD
DIIATFFLNLFHGGSLKAMPPKLLSSDKKNILIRPLAYVEEKDIIKYADLRKFPIIPCNLCGSQENLQRAMLNEMLREWD
KQYPKRLHSIFGALQNVSPSQLADRDLFDFEILDSQRELDFKDPEEMKKRLDIVNLSFAAD
>Mature_301_residues
MYSPVESEQGFNFKPELPTSSAYYRLLKKLRRQVGHAIRDFKMIEDGDKVMVCVSGGKDSYTLLDILLQFKRIAPINFDI
VAVNLDQKQPGFPEDVLPRYMEENNIPYYILEKDTYTITKRLTPEGKTYCAVCSRLRRGSLYGFAQEIGATKVALGHHRD
DIIATFFLNLFHGGSLKAMPPKLLSSDKKNILIRPLAYVEEKDIIKYADLRKFPIIPCNLCGSQENLQRAMLNEMLREWD
KQYPKRLHSIFGALQNVSPSQLADRDLFDFEILDSQRELDFKDPEEMKKRLDIVNLSFAAD

Specific function: Required for the thiolation of cytidine in position 32 of tRNA, to form 2-thiocytidine (s(2)C32)

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TtcA family

Homologues:

Organism=Escherichia coli, GI1787606, Length=255, Percent_Identity=62.3529411764706, Blast_Score=350, Evalue=5e-98,
Organism=Caenorhabditis elegans, GI193211090, Length=233, Percent_Identity=32.618025751073, Blast_Score=130, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI17540090, Length=234, Percent_Identity=26.9230769230769, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24651972, Length=278, Percent_Identity=24.4604316546763, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TTCA_ACIAD (Q6F8Q3)

Other databases:

- EMBL:   CR543861
- RefSeq:   YP_047384.1
- ProteinModelPortal:   Q6F8Q3
- SMR:   Q6F8Q3
- STRING:   Q6F8Q3
- GeneID:   2878101
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD2829
- NMPDR:   fig|62977.3.peg.2577
- eggNOG:   COG0037
- HOGENOM:   HBG347038
- OMA:   EHILPEY
- PhylomeDB:   Q6F8Q3
- ProtClustDB:   PRK10696
- BioCyc:   ASP62977:ACIAD2829-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01850
- InterPro:   IPR012089
- InterPro:   IPR014729
- InterPro:   IPR011063
- Gene3D:   G3DSA:3.40.50.620
- PIRSF:   PIRSF004976

Pfam domain/function: PF01171 ATP_bind_3

EC number: NA

Molecular weight: Translated: 34714; Mature: 34714

Theoretical pI: Translated: 7.87; Mature: 7.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYSPVESEQGFNFKPELPTSSAYYRLLKKLRRQVGHAIRDFKMIEDGDKVMVCVSGGKDS
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCEEEEEEECCCCC
YTLLDILLQFKRIAPINFDIVAVNLDQKQPGFPEDVLPRYMEENNIPYYILEKDTYTITK
HHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCHHHHHHHHHCCCCCEEEECCCCEEEEE
RLTPEGKTYCAVCSRLRRGSLYGFAQEIGATKVALGHHRDDIIATFFLNLFHGGSLKAMP
ECCCCCCHHHHHHHHHHCCCHHHHHHHHCCHHEECCCCHHHHHHHHHHHHHCCCCCCCCC
PKLLSSDKKNILIRPLAYVEEKDIIKYADLRKFPIIPCNLCGSQENLQRAMLNEMLREWD
HHHHCCCCCCEEECHHHHHCHHHHHHHHCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHH
KQYPKRLHSIFGALQNVSPSQLADRDLFDFEILDSQRELDFKDPEEMKKRLDIVNLSFAA
HHHHHHHHHHHHHHHCCCHHHHHCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHEEEEEC
D
C
>Mature Secondary Structure
MYSPVESEQGFNFKPELPTSSAYYRLLKKLRRQVGHAIRDFKMIEDGDKVMVCVSGGKDS
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCEEEEEEECCCCC
YTLLDILLQFKRIAPINFDIVAVNLDQKQPGFPEDVLPRYMEENNIPYYILEKDTYTITK
HHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCHHHHHHHHHCCCCCEEEECCCCEEEEE
RLTPEGKTYCAVCSRLRRGSLYGFAQEIGATKVALGHHRDDIIATFFLNLFHGGSLKAMP
ECCCCCCHHHHHHHHHHCCCHHHHHHHHCCHHEECCCCHHHHHHHHHHHHHCCCCCCCCC
PKLLSSDKKNILIRPLAYVEEKDIIKYADLRKFPIIPCNLCGSQENLQRAMLNEMLREWD
HHHHCCCCCCEEECHHHHHCHHHHHHHHCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHH
KQYPKRLHSIFGALQNVSPSQLADRDLFDFEILDSQRELDFKDPEEMKKRLDIVNLSFAA
HHHHHHHHHHHHHHHCCCHHHHHCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHEEEEEC
D
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA