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The map label for this gene is nemA [H]

Identifier: 50085787

GI number: 50085787

Start: 2671209

End: 2672282

Strand: Reverse

Name: nemA [H]

Synonym: ACIAD2725

Alternate gene names: 50085787

Gene position: 2672282-2671209 (Counterclockwise)

Preceding gene: 50085789

Following gene: 50085786

Centisome position: 74.26

GC content: 40.78

Gene sequence:

>1074_bases
ATGAATCAAATATTTAAACCTATTCTTATCGGTCAGTATCAATTAGCAAACCGACTAGCTATGGCACCAATGACACGATC
ACGTGCTCAGTTTGATGGCACACCTAGCGAATTAGCAGCTGAGTATTATACTCAGCGTGCAAGTGTGGGATTAATCATTG
CTGAAGGTGCACAGCCCTCTGAAGATGGTCAGGGCTATTTAGCAACGCCGGGTATTTATACAGATTCACATATTGCGGGC
TGGAAAAAAATCACCAATGCAATACATGCTAAAGGTGGACGCATTTTCATTCAATTGATGCATGTTGGTCGTATGTCACA
TCCTGATAACACCCCGCATCATCGTCAAGCTGTTGCTCCTTCATCTATTGCTCCAGGTACAGTTACATTCACACCCACTG
GAATGCAAGAAATTCCGAAACCTCGTGCATTGACGATTAAAGAAATTAAACAGACTATCCAGGATTTTAGACATGCTGCT
CGATCTGCAATTGAAGCGGGTGCGGATGGTGTGGAAATTCATGGCGCCAATGCTTATCTATTTCAGCAATTTTTTGCACC
AACGGCAAATACCCGTACAGATGAATATGGTGGTTCTATTGAGAATCGCGCTCGATTTGCTATTGAAGTAGCAACTGCTA
TTGCAGAAGAAATTGGTGCTGATAAAACTGCGATTCGTCTTTCTCCAGGTTCTTTGCTTTGGGGAATTGATGAAGGTCAA
GAAGGCCCAGAATTATATCATTATCTTGTAAAAGAGCTTAATAAACTTGGACTTGTGTATCTACACATTATGCATCTTGG
CAATGAAACATTGTTGCAAGATATACGTCAACTATGGACACAGGCATTAATAGTTAACCGGCCCGGACGTCCGCGTGAAC
AGATTGGAACAGATGTTGAATCAGGTTTAGCAGATTTAGAGTCTTATGGCGAAATGATTCTTGCAAACCCAGATTTTGTA
AATCGTCTGCAACAAAATTTACCAATGAATGAAGCAGATAAAACGACATATTACGGTGGTGATGCAAAAGGTTATGTCGA
TTATCCAGCTTTAAATGCATCTGATAAAATTTAA

Upstream 100 bases:

>100_bases
CTCGTCAGCTTGTTGCAGCAGATGATGGTGAAGTTCGTATCTAAGAAAATTAGATTAAGTTGCATCAAATTATCTAAATC
AATTTATTGGACAGTGAATC

Downstream 100 bases:

>100_bases
AGGCTGCAATTATGTCTATTGTTATCGCTAAAGTTCAACGCGCTAGCCATGGTAATCAATTTTATGGCTATGGATTACGT
GCAGAAGAATCGCTAATTTC

Product: oxidoreductase, NADH-dependent flavin oxidoreductase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MNQIFKPILIGQYQLANRLAMAPMTRSRAQFDGTPSELAAEYYTQRASVGLIIAEGAQPSEDGQGYLATPGIYTDSHIAG
WKKITNAIHAKGGRIFIQLMHVGRMSHPDNTPHHRQAVAPSSIAPGTVTFTPTGMQEIPKPRALTIKEIKQTIQDFRHAA
RSAIEAGADGVEIHGANAYLFQQFFAPTANTRTDEYGGSIENRARFAIEVATAIAEEIGADKTAIRLSPGSLLWGIDEGQ
EGPELYHYLVKELNKLGLVYLHIMHLGNETLLQDIRQLWTQALIVNRPGRPREQIGTDVESGLADLESYGEMILANPDFV
NRLQQNLPMNEADKTTYYGGDAKGYVDYPALNASDKI

Sequences:

>Translated_357_residues
MNQIFKPILIGQYQLANRLAMAPMTRSRAQFDGTPSELAAEYYTQRASVGLIIAEGAQPSEDGQGYLATPGIYTDSHIAG
WKKITNAIHAKGGRIFIQLMHVGRMSHPDNTPHHRQAVAPSSIAPGTVTFTPTGMQEIPKPRALTIKEIKQTIQDFRHAA
RSAIEAGADGVEIHGANAYLFQQFFAPTANTRTDEYGGSIENRARFAIEVATAIAEEIGADKTAIRLSPGSLLWGIDEGQ
EGPELYHYLVKELNKLGLVYLHIMHLGNETLLQDIRQLWTQALIVNRPGRPREQIGTDVESGLADLESYGEMILANPDFV
NRLQQNLPMNEADKTTYYGGDAKGYVDYPALNASDKI
>Mature_357_residues
MNQIFKPILIGQYQLANRLAMAPMTRSRAQFDGTPSELAAEYYTQRASVGLIIAEGAQPSEDGQGYLATPGIYTDSHIAG
WKKITNAIHAKGGRIFIQLMHVGRMSHPDNTPHHRQAVAPSSIAPGTVTFTPTGMQEIPKPRALTIKEIKQTIQDFRHAA
RSAIEAGADGVEIHGANAYLFQQFFAPTANTRTDEYGGSIENRARFAIEVATAIAEEIGADKTAIRLSPGSLLWGIDEGQ
EGPELYHYLVKELNKLGLVYLHIMHLGNETLLQDIRQLWTQALIVNRPGRPREQIGTDVESGLADLESYGEMILANPDFV
NRLQQNLPMNEADKTTYYGGDAKGYVDYPALNASDKI

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=361, Percent_Identity=41.2742382271468, Blast_Score=256, Evalue=1e-69,
Organism=Escherichia coli, GI1789463, Length=228, Percent_Identity=35.9649122807018, Blast_Score=122, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI17559804, Length=142, Percent_Identity=44.3661971830986, Blast_Score=100, Evalue=9e-22,
Organism=Caenorhabditis elegans, GI17565138, Length=223, Percent_Identity=33.6322869955157, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17540738, Length=134, Percent_Identity=40.2985074626866, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI72001454, Length=134, Percent_Identity=39.5522388059701, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17559802, Length=128, Percent_Identity=42.96875, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17564188, Length=143, Percent_Identity=37.7622377622378, Blast_Score=85, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17566914, Length=217, Percent_Identity=28.5714285714286, Blast_Score=80, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6325086, Length=384, Percent_Identity=37.7604166666667, Blast_Score=210, Evalue=2e-55,
Organism=Saccharomyces cerevisiae, GI6321973, Length=386, Percent_Identity=36.0103626943005, Blast_Score=208, Evalue=1e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 39257; Mature: 39257

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQIFKPILIGQYQLANRLAMAPMTRSRAQFDGTPSELAAEYYTQRASVGLIIAEGAQPS
CCCHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHHHCCEEEEEECCCCCC
EDGQGYLATPGIYTDSHIAGWKKITNAIHAKGGRIFIQLMHVGRMSHPDNTPHHRQAVAP
CCCCCEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEHHCCCCCCCCCCCHHHHCCC
SSIAPGTVTFTPTGMQEIPKPRALTIKEIKQTIQDFRHAARSAIEAGADGVEIHGANAYL
CCCCCCEEEECCCCHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHH
FQQFFAPTANTRTDEYGGSIENRARFAIEVATAIAEEIGADKTAIRLSPGSLLWGIDEGQ
HHHHHCCCCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEECCCCC
EGPELYHYLVKELNKLGLVYLHIMHLGNETLLQDIRQLWTQALIVNRPGRPREQIGTDVE
CCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCHH
SGLADLESYGEMILANPDFVNRLQQNLPMNEADKTTYYGGDAKGYVDYPALNASDKI
HHHHHHHHHCCEEEECCHHHHHHHHHCCCCCCCCCEEECCCCCCEECCCCCCCCCCC
>Mature Secondary Structure
MNQIFKPILIGQYQLANRLAMAPMTRSRAQFDGTPSELAAEYYTQRASVGLIIAEGAQPS
CCCHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHHHCCEEEEEECCCCCC
EDGQGYLATPGIYTDSHIAGWKKITNAIHAKGGRIFIQLMHVGRMSHPDNTPHHRQAVAP
CCCCCEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEHHCCCCCCCCCCCHHHHCCC
SSIAPGTVTFTPTGMQEIPKPRALTIKEIKQTIQDFRHAARSAIEAGADGVEIHGANAYL
CCCCCCEEEECCCCHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHH
FQQFFAPTANTRTDEYGGSIENRARFAIEVATAIAEEIGADKTAIRLSPGSLLWGIDEGQ
HHHHHCCCCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEECCCCC
EGPELYHYLVKELNKLGLVYLHIMHLGNETLLQDIRQLWTQALIVNRPGRPREQIGTDVE
CCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCHH
SGLADLESYGEMILANPDFVNRLQQNLPMNEADKTTYYGGDAKGYVDYPALNASDKI
HHHHHHHHHCCEEEECCHHHHHHHHHCCCCCCCCCEEECCCCCCEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]