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The map label for this gene is thcR [H]

Identifier: 50085739

GI number: 50085739

Start: 2626535

End: 2627671

Strand: Direct

Name: thcR [H]

Synonym: ACIAD2672

Alternate gene names: 50085739

Gene position: 2626535-2627671 (Clockwise)

Preceding gene: 50085738

Following gene: 50085759

Centisome position: 72.99

GC content: 40.46

Gene sequence:

>1137_bases
TTGCTGGCAACATTTCAATCTGGTCATTTGGTCGACACAGTTGTGATGTTTTCAGTAAGCCAGCCAAAAGGAATATGTAT
GAGTCAGGTCAATCCCGCTATGGAATTAAATGGACGCCATCTACAACAATGTTCCATTTTTTCATCACAAAACTTGGTGT
TTCAACATCAGGACTTAGGCGAAACCTGCAAAAGTGTGGGACAAATCTTTAAGCCACATCTGTTAAAAATTGTGCAACAA
CGCAACGATTTTTACTCATCTATGCACCATCTTAAGGCAGGTCGACTATCCATTAGTCGTCTTGAATACGGCGCCGACGT
TTATATTGAGCCTGATCACTTAGAAAAATTTTATTTGATCCAGATTCCGATGCAGGGCTATGCCGAAATTGAAATTAATC
GGCAAAAATTTATTTCTTATACACAAGTTGCATCACTCATTTCTCCCGATCAATCTTTGAAAATGCGCTGGCATGCAAAC
TCTCCTCAGTTAATTCTCAAAATTGATAAAGAAGATCTTTTACAGCATTGTCGTCAGCATCTTCCAGATGCACCACATCA
TTTGCCCTTATTTGAGCCTAAACTTGATTTTTCGACTCAGGGCGGAGCCTACTTCCTACAACTGATGCGTACCCTCATCG
ATGCCTTAGGCTGTGAACAACATCCGTTGCATCATCCAATGGCCTTCAAACAATTTGAATCCAGTCTGTTTAATGCGCTG
ATTTATGGACAACAAAATACTTTATTAGAACGTATTAAACAGGCCAAAGAACAAGCAATTTCTCCTTATTTTATCAAACG
CACCGAAGCGTTTATGCGTGAAAATCTGCATGCATCGCTCACCATTGAGCAATTGGCCGAACATGCTGGTGTCAGTGTAC
GTACCTTATTTGCAGGATTTAAAAGCTTTTTGAACACCACTCCAATGGCTTATCTCAAAGAATTGCGTTTTGAACAGGCA
CATACTGAATTGTTACGTAACGAGGATGTTTCAGTCACTGATGTTGCATTCAAATGGGGTTTTACTCATCTTGGTCGTTT
TTCTCAAGAATATAAACGCCGCTATGGTGAATTACCATCGTGCACGCGCCGTGCCACCATAGAACATCAGGATATGGGCT
TAAGAGCATATTCATAA

Upstream 100 bases:

>100_bases
CATGCCTAAGCAGGTTTAAAAATAGTATTCCAACTGCTTTAGTTGGTCTATTTTTTGCTTAATTCTTTATGTCATTGCGA
GCCACTTAAATGTTTAAGTA

Downstream 100 bases:

>100_bases
TCAAACTAAAATATTTGACGACATCGAAATAAATCATCTGTTCTAAATTTATTTTGAGTAGAGGTTTTGCCTCAAGCCCA
TGATCTATAGACCCAATTTT

Product: transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 378; Mature: 378

Protein sequence:

>378_residues
MLATFQSGHLVDTVVMFSVSQPKGICMSQVNPAMELNGRHLQQCSIFSSQNLVFQHQDLGETCKSVGQIFKPHLLKIVQQ
RNDFYSSMHHLKAGRLSISRLEYGADVYIEPDHLEKFYLIQIPMQGYAEIEINRQKFISYTQVASLISPDQSLKMRWHAN
SPQLILKIDKEDLLQHCRQHLPDAPHHLPLFEPKLDFSTQGGAYFLQLMRTLIDALGCEQHPLHHPMAFKQFESSLFNAL
IYGQQNTLLERIKQAKEQAISPYFIKRTEAFMRENLHASLTIEQLAEHAGVSVRTLFAGFKSFLNTTPMAYLKELRFEQA
HTELLRNEDVSVTDVAFKWGFTHLGRFSQEYKRRYGELPSCTRRATIEHQDMGLRAYS

Sequences:

>Translated_378_residues
MLATFQSGHLVDTVVMFSVSQPKGICMSQVNPAMELNGRHLQQCSIFSSQNLVFQHQDLGETCKSVGQIFKPHLLKIVQQ
RNDFYSSMHHLKAGRLSISRLEYGADVYIEPDHLEKFYLIQIPMQGYAEIEINRQKFISYTQVASLISPDQSLKMRWHAN
SPQLILKIDKEDLLQHCRQHLPDAPHHLPLFEPKLDFSTQGGAYFLQLMRTLIDALGCEQHPLHHPMAFKQFESSLFNAL
IYGQQNTLLERIKQAKEQAISPYFIKRTEAFMRENLHASLTIEQLAEHAGVSVRTLFAGFKSFLNTTPMAYLKELRFEQA
HTELLRNEDVSVTDVAFKWGFTHLGRFSQEYKRRYGELPSCTRRATIEHQDMGLRAYS
>Mature_378_residues
MLATFQSGHLVDTVVMFSVSQPKGICMSQVNPAMELNGRHLQQCSIFSSQNLVFQHQDLGETCKSVGQIFKPHLLKIVQQ
RNDFYSSMHHLKAGRLSISRLEYGADVYIEPDHLEKFYLIQIPMQGYAEIEINRQKFISYTQVASLISPDQSLKMRWHAN
SPQLILKIDKEDLLQHCRQHLPDAPHHLPLFEPKLDFSTQGGAYFLQLMRTLIDALGCEQHPLHHPMAFKQFESSLFNAL
IYGQQNTLLERIKQAKEQAISPYFIKRTEAFMRENLHASLTIEQLAEHAGVSVRTLFAGFKSFLNTTPMAYLKELRFEQA
HTELLRNEDVSVTDVAFKWGFTHLGRFSQEYKRRYGELPSCTRRATIEHQDMGLRAYS

Specific function: Probably involved in the positive regulation of the thc operon for the degradation of the thiocarbamate herbicide EPTC [H]

COG id: COG2207

COG function: function code K; AraC-type DNA-binding domain-containing proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788778, Length=190, Percent_Identity=26.8421052631579, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060 [H]

Pfam domain/function: PF00165 HTH_AraC [H]

EC number: NA

Molecular weight: Translated: 43616; Mature: 43616

Theoretical pI: Translated: 7.96; Mature: 7.96

Prosite motif: PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLATFQSGHLVDTVVMFSVSQPKGICMSQVNPAMELNGRHLQQCSIFSSQNLVFQHQDLG
CCCCCCCCCHHHHHHEEECCCCCCCHHHHCCCCHHCCCCHHHHHHHCCCCCEEEEHHHHH
ETCKSVGQIFKPHLLKIVQQRNDFYSSMHHLKAGRLSISRLEYGADVYIEPDHLEKFYLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEHHHHHCCCCEEECHHCCCEEEEE
QIPMQGYAEIEINRQKFISYTQVASLISPDQSLKMRWHANSPQLILKIDKEDLLQHCRQH
EECCCCCEEEEECHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEECHHHHHHHHHHH
LPDAPHHLPLFEPKLDFSTQGGAYFLQLMRTLIDALGCEQHPLHHPMAFKQFESSLFNAL
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
IYGQQNTLLERIKQAKEQAISPYFIKRTEAFMRENLHASLTIEQLAEHAGVSVRTLFAGF
HHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEHHHHHHHCCCHHHHHHHHH
KSFLNTTPMAYLKELRFEQAHTELLRNEDVSVTDVAFKWGFTHLGRFSQEYKRRYGELPS
HHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
CTRRATIEHQDMGLRAYS
CHHHCCCCHHCCCCCCCC
>Mature Secondary Structure
MLATFQSGHLVDTVVMFSVSQPKGICMSQVNPAMELNGRHLQQCSIFSSQNLVFQHQDLG
CCCCCCCCCHHHHHHEEECCCCCCCHHHHCCCCHHCCCCHHHHHHHCCCCCEEEEHHHHH
ETCKSVGQIFKPHLLKIVQQRNDFYSSMHHLKAGRLSISRLEYGADVYIEPDHLEKFYLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEHHHHHCCCCEEECHHCCCEEEEE
QIPMQGYAEIEINRQKFISYTQVASLISPDQSLKMRWHANSPQLILKIDKEDLLQHCRQH
EECCCCCEEEEECHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEEEECHHHHHHHHHHH
LPDAPHHLPLFEPKLDFSTQGGAYFLQLMRTLIDALGCEQHPLHHPMAFKQFESSLFNAL
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
IYGQQNTLLERIKQAKEQAISPYFIKRTEAFMRENLHASLTIEQLAEHAGVSVRTLFAGF
HHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCEEEHHHHHHHCCCHHHHHHHHH
KSFLNTTPMAYLKELRFEQAHTELLRNEDVSVTDVAFKWGFTHLGRFSQEYKRRYGELPS
HHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
CTRRATIEHQDMGLRAYS
CHHHCCCCHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7836301 [H]