Click here to switch to the map view.

The map label for this gene is radA [H]

Identifier: 50085731

GI number: 50085731

Start: 2617574

End: 2618953

Strand: Direct

Name: radA [H]

Synonym: ACIAD2664

Alternate gene names: 50085731

Gene position: 2617574-2618953 (Clockwise)

Preceding gene: 50085720

Following gene: 50085732

Centisome position: 72.74

GC content: 45.94

Gene sequence:

>1380_bases
ATGAGCAAAGTCAAAACCGTTTATCGCTGTGAGCAATGTGGAGCCGATCACCCTAAATGGGCTGGACAATGTTCTGAGTG
TGGAGAATGGAACAGTTTGACTGAAGTTCGGCTTGAAACCAGTACCAATCATCGCGCCAAACCCAAACTCGGTGGTTATG
CAGGAGAAATTTCAACTGTTACTACCTTAAATCAGATCTCTGTTTCACACGAAACACGGGTGGCCACTGGAATTGGTGAG
TTTGATCGAGTGCTGGGTGGGGGGTTAGTCACAGGCTCAGTTGTACTGATTGGCGGAGATCCTGGTATTGGTAAATCGAC
CATTTTGCTGCAAACAGCAACTTATATGGCAAGCAGCAATAGCTCTGCACTTTATGTGACAGGCGAAGAATCATTATCTC
AAGTGGCTTTACGTGCCCAAAGGCTCGACTTACCGACAGACAAATTGAAAGTCATGGCTGAAACTTGTGTCGAGCGTATT
TGTGAAATATTAGCACATGAACGCCCTACCGTTGCTATATTAGATTCGATTCAAACACTTTACACCGAAACATTACAGTC
CGCACCAGGTGGCGTATCTCAAATCCGAGAATCGGCAGCCTTACTGACTCGTTTTGCAAAACATAGCGGTACTGCGCTGT
TTATTGTGGGGCATGTGACTAAAGAAGGTGCTCTTGCTGGTCCGCGCGTGCTTGAACATATGGTCGATTGTGTCCTATAT
TTTGAAGGACAGTCAGATTCAAGATATCGCATGATTCGTGCTGTTAAGAACCGTTTTGGTGCGGTGAATGAACTGGGCGT
TTTTGGTATGACCGATAAGGGACTTCGCGAAGTTGCCAATCCTTCTGCTATTTTTCTGAGTCGTTACGATGAGGCAATTC
CAGGTTCAATTGTGATGATTAGCCGTGAAGGTACTCGACCACTCTTAGTTGAAGTACAAGCACTGGTGGATGATGCTCAA
GGACAACCGCGCCGTGTCGCTTTGGGCTTGGAGCAAAATCGTCTCAATATGCTTTTGGCTGTGATGCATCGTCATGGAGG
AGTACAAACTTCTGGTCAGGATGTCTATGTCAATGTCGTGGGTGGATTAAAAATTACCGAAACGGGTTCTGATCTTGCAG
TCCTTCTCGCCTGTGCCTCTAGTTTAAGAGGAAAAGCTTTGCCTCAACAGCTTGCTGTATTTGGTGAAGTTGGCCTCTCT
GGTGAAATTCGTCCTGTCCCTAATGGTCAGGAGCGCTTAAAAGAAGCGACAAAACATGGTTTCACACACATCATTTTACC
TCGTGGTAATGCACCTCAGAAAAAAATTGAAGGTGTACACGTCATTGCGGTAGCGCGCTTACATGAAGCCTTGCAAACTG
CAATGGAGCTTTCTTCATGA

Upstream 100 bases:

>100_bases
CGTGCCAAACTTAAATTAGGTGATGTTAAGCTAAAAATTTGTATTTCTGGCACGCTATTGCTACATTTCGTCATCAACGC
CATCTAAATATGCACCAAAT

Downstream 100 bases:

>100_bases
GATAAGGATGTGGTTCAAGTTCTCAAATTCGGTGAGTTATTTTCATCATGTTATGCACTTCTTAAGCTATAGCATGATGA
GGTCTTTAGATCATTTGCTA

Product: DNA repair protein RadA

Products: NA

Alternate protein names: DNA repair protein sms homolog [H]

Number of amino acids: Translated: 459; Mature: 458

Protein sequence:

>459_residues
MSKVKTVYRCEQCGADHPKWAGQCSECGEWNSLTEVRLETSTNHRAKPKLGGYAGEISTVTTLNQISVSHETRVATGIGE
FDRVLGGGLVTGSVVLIGGDPGIGKSTILLQTATYMASSNSSALYVTGEESLSQVALRAQRLDLPTDKLKVMAETCVERI
CEILAHERPTVAILDSIQTLYTETLQSAPGGVSQIRESAALLTRFAKHSGTALFIVGHVTKEGALAGPRVLEHMVDCVLY
FEGQSDSRYRMIRAVKNRFGAVNELGVFGMTDKGLREVANPSAIFLSRYDEAIPGSIVMISREGTRPLLVEVQALVDDAQ
GQPRRVALGLEQNRLNMLLAVMHRHGGVQTSGQDVYVNVVGGLKITETGSDLAVLLACASSLRGKALPQQLAVFGEVGLS
GEIRPVPNGQERLKEATKHGFTHIILPRGNAPQKKIEGVHVIAVARLHEALQTAMELSS

Sequences:

>Translated_459_residues
MSKVKTVYRCEQCGADHPKWAGQCSECGEWNSLTEVRLETSTNHRAKPKLGGYAGEISTVTTLNQISVSHETRVATGIGE
FDRVLGGGLVTGSVVLIGGDPGIGKSTILLQTATYMASSNSSALYVTGEESLSQVALRAQRLDLPTDKLKVMAETCVERI
CEILAHERPTVAILDSIQTLYTETLQSAPGGVSQIRESAALLTRFAKHSGTALFIVGHVTKEGALAGPRVLEHMVDCVLY
FEGQSDSRYRMIRAVKNRFGAVNELGVFGMTDKGLREVANPSAIFLSRYDEAIPGSIVMISREGTRPLLVEVQALVDDAQ
GQPRRVALGLEQNRLNMLLAVMHRHGGVQTSGQDVYVNVVGGLKITETGSDLAVLLACASSLRGKALPQQLAVFGEVGLS
GEIRPVPNGQERLKEATKHGFTHIILPRGNAPQKKIEGVHVIAVARLHEALQTAMELSS
>Mature_458_residues
SKVKTVYRCEQCGADHPKWAGQCSECGEWNSLTEVRLETSTNHRAKPKLGGYAGEISTVTTLNQISVSHETRVATGIGEF
DRVLGGGLVTGSVVLIGGDPGIGKSTILLQTATYMASSNSSALYVTGEESLSQVALRAQRLDLPTDKLKVMAETCVERIC
EILAHERPTVAILDSIQTLYTETLQSAPGGVSQIRESAALLTRFAKHSGTALFIVGHVTKEGALAGPRVLEHMVDCVLYF
EGQSDSRYRMIRAVKNRFGAVNELGVFGMTDKGLREVANPSAIFLSRYDEAIPGSIVMISREGTRPLLVEVQALVDDAQG
QPRRVALGLEQNRLNMLLAVMHRHGGVQTSGQDVYVNVVGGLKITETGSDLAVLLACASSLRGKALPQQLAVFGEVGLSG
EIRPVPNGQERLKEATKHGFTHIILPRGNAPQKKIEGVHVIAVARLHEALQTAMELSS

Specific function: May play a role in the repair of endogenous alkylation damage [H]

COG id: COG1066

COG function: function code O; Predicted ATP-dependent serine protease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recA family. RadA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790850, Length=456, Percent_Identity=58.7719298245614, Blast_Score=553, Evalue=1e-159,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR014774
- InterPro:   IPR004504
- InterPro:   IPR008269
- InterPro:   IPR020568 [H]

Pfam domain/function: PF06745 KaiC; PF05362 Lon_C [H]

EC number: NA

Molecular weight: Translated: 49312; Mature: 49181

Theoretical pI: Translated: 7.59; Mature: 7.59

Prosite motif: PS50162 RECA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKVKTVYRCEQCGADHPKWAGQCSECGEWNSLTEVRLETSTNHRAKPKLGGYAGEISTV
CCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCHHH
TTLNQISVSHETRVATGIGEFDRVLGGGLVTGSVVLIGGDPGIGKSTILLQTATYMASSN
EEHHHHCCCCCHHHHHCCHHHHHHHCCCEEECEEEEEECCCCCCCCEEEEEEHHHHCCCC
SSALYVTGEESLSQVALRAQRLDLPTDKLKVMAETCVERICEILAHERPTVAILDSIQTL
CCEEEEECHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
YTETLQSAPGGVSQIRESAALLTRFAKHSGTALFIVGHVTKEGALAGPRVLEHMVDCVLY
HHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCHHHHHHHHHEEEE
FEGQSDSRYRMIRAVKNRFGAVNELGVFGMTDKGLREVANPSAIFLSRYDEAIPGSIVMI
EECCCCCHHHHHHHHHHHHCCHHHCCEECCCCHHHHHHCCCCEEEEEHHCCCCCCCEEEE
SREGTRPLLVEVQALVDDAQGQPRRVALGLEQNRLNMLLAVMHRHGGVQTSGQDVYVNVV
ECCCCCCHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEE
GGLKITETGSDLAVLLACASSLRGKALPQQLAVFGEVGLSGEIRPVPNGQERLKEATKHG
CCEEEEECCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCC
FTHIILPRGNAPQKKIEGVHVIAVARLHEALQTAMELSS
CEEEEEECCCCCHHHHCCEEEEHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SKVKTVYRCEQCGADHPKWAGQCSECGEWNSLTEVRLETSTNHRAKPKLGGYAGEISTV
CCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCHHH
TTLNQISVSHETRVATGIGEFDRVLGGGLVTGSVVLIGGDPGIGKSTILLQTATYMASSN
EEHHHHCCCCCHHHHHCCHHHHHHHCCCEEECEEEEEECCCCCCCCEEEEEEHHHHCCCC
SSALYVTGEESLSQVALRAQRLDLPTDKLKVMAETCVERICEILAHERPTVAILDSIQTL
CCEEEEECHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
YTETLQSAPGGVSQIRESAALLTRFAKHSGTALFIVGHVTKEGALAGPRVLEHMVDCVLY
HHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCHHHHHHHHHEEEE
FEGQSDSRYRMIRAVKNRFGAVNELGVFGMTDKGLREVANPSAIFLSRYDEAIPGSIVMI
EECCCCCHHHHHHHHHHHHCCHHHCCEECCCCHHHHHHCCCCEEEEEHHCCCCCCCEEEE
SREGTRPLLVEVQALVDDAQGQPRRVALGLEQNRLNMLLAVMHRHGGVQTSGQDVYVNVV
ECCCCCCHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEE
GGLKITETGSDLAVLLACASSLRGKALPQQLAVFGEVGLSGEIRPVPNGQERLKEATKHG
CCEEEEECCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCC
FTHIILPRGNAPQKKIEGVHVIAVARLHEALQTAMELSS
CEEEEEECCCCCHHHHCCEEEEHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]