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The map label for this gene is tolB

Identifier: 50085692

GI number: 50085692

Start: 2575393

End: 2576670

Strand: Direct

Name: tolB

Synonym: ACIAD2622

Alternate gene names: 50085692

Gene position: 2575393-2576670 (Clockwise)

Preceding gene: 50085691

Following gene: 50085693

Centisome position: 71.57

GC content: 43.97

Gene sequence:

>1278_bases
ATGACGCGCAAACATATACTTTCATTTGCCCTGATGACGGCACTCGGCATGACCGTTACTTCTACAGCCTTTGCACAATT
ACATTTAGAAATTGCCAAAGCGCCAGAACAGGCTCCTAAAATCGCGATTGTGCCTTTTGGGAATGACCAGTCAATTTTTC
CTATTGTTGAAAATGACCTGAATCGATCAGGCCGTTTTAGCAGCGCATCAAAAAATCTACCAACCACAGCCTCAATTGAT
GCGATTCAGGCATCTGCATGGCAGGCTGCAGGTATCCCCTATGTAGTTGTTGGCAATCTAAAACCTGCTGCCAACAATGC
ATTTGAAGTGCATTACCAACTTTATGATGTACAAAAACAGCAATATCTGCTCAATGAAGTTCTAACTGTACCAGCATCAC
GTGTGCGACAGGCAGCGCATATGATTAGTGATGCCATTTATCAGGCACTTACTGGTATTGCTGGAGATTTTAGCGGACGT
ATCGCATATGTATTACGCAATCCTGCAACGCCAGATCAACGTTATACCTTACAGATTGCGGATACCGATGGTGAGCAGCC
TAAAACAGTCTTATCTTCACGCGATCCTATCTTGTCTCCTGCATGGACACCTGATGCCAAAAAAATTGCCTATGTTTCAT
TTGAAACCAAACGCCCAGCGATTTATTTACAAGACCTTGCTACTGGTCAGCGCGAAGTCCTCGCCAGCTTCAAAGGTTTA
AATGGCGCACCAAGCTTCTCTCCAGATGGTAAATCGATGTTATTTACAGCATCGATGAATGGTAATCCAGAAATCTATCA
AATGGATTTATCAACAAGACAATTGCAACGCATGACCAATGACAATGCTATTGACACAGAAGCACGTTACGCACCAGATG
GTAAATCATTCATCTTTACATCGGATCGCGGTGGTTCTCCGCAAATTTATCGATACAGTTTTGATGACTCATCAACTAAA
CGTTTGACCTTCCGTGGTGCATTTAATGCACGTGGTACGCTCAGCGCTGATGGTAAAAATATCGCGCTGGTTCATCGCCC
TTCTGGCAGCAATTACAAAGTTGCCATCATGAATATTTCGACTGGTATTGTAAATATTCTGACCCCAACCAGCCTGGATG
AATCACCAAGCTTTTCTCCAAATGGACAAATGGTGGTGTATGCAACCTATGAAGGGAGTCGTGGCTTGCTCTCTATCATG
TCGACTGATGGACGTTTCCGTATGAATCTTCCAAGCGAACAAGGCGAAGTAAGAGAACCAGCTTGGGCACCAAAGTAA

Upstream 100 bases:

>100_bases
TTGCTCACGATTACATGCTTTACATGGATTCAAAGTATGTAATCGTGCTAAAACAACACACGAGCAACTCAAACCAACCC
AAGCTATCACGATTATGGAT

Downstream 100 bases:

>100_bases
TTACCTGATATCACCTCCATGGAGATAAAAATGAACGCTATGAAACTATTTGTTTTACCTGTTTTGGCTACGGCTTTAGT
AATGACAGGTTGTGCAAGCC

Product: translocation protein TolB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 425; Mature: 424

Protein sequence:

>425_residues
MTRKHILSFALMTALGMTVTSTAFAQLHLEIAKAPEQAPKIAIVPFGNDQSIFPIVENDLNRSGRFSSASKNLPTTASID
AIQASAWQAAGIPYVVVGNLKPAANNAFEVHYQLYDVQKQQYLLNEVLTVPASRVRQAAHMISDAIYQALTGIAGDFSGR
IAYVLRNPATPDQRYTLQIADTDGEQPKTVLSSRDPILSPAWTPDAKKIAYVSFETKRPAIYLQDLATGQREVLASFKGL
NGAPSFSPDGKSMLFTASMNGNPEIYQMDLSTRQLQRMTNDNAIDTEARYAPDGKSFIFTSDRGGSPQIYRYSFDDSSTK
RLTFRGAFNARGTLSADGKNIALVHRPSGSNYKVAIMNISTGIVNILTPTSLDESPSFSPNGQMVVYATYEGSRGLLSIM
STDGRFRMNLPSEQGEVREPAWAPK

Sequences:

>Translated_425_residues
MTRKHILSFALMTALGMTVTSTAFAQLHLEIAKAPEQAPKIAIVPFGNDQSIFPIVENDLNRSGRFSSASKNLPTTASID
AIQASAWQAAGIPYVVVGNLKPAANNAFEVHYQLYDVQKQQYLLNEVLTVPASRVRQAAHMISDAIYQALTGIAGDFSGR
IAYVLRNPATPDQRYTLQIADTDGEQPKTVLSSRDPILSPAWTPDAKKIAYVSFETKRPAIYLQDLATGQREVLASFKGL
NGAPSFSPDGKSMLFTASMNGNPEIYQMDLSTRQLQRMTNDNAIDTEARYAPDGKSFIFTSDRGGSPQIYRYSFDDSSTK
RLTFRGAFNARGTLSADGKNIALVHRPSGSNYKVAIMNISTGIVNILTPTSLDESPSFSPNGQMVVYATYEGSRGLLSIM
STDGRFRMNLPSEQGEVREPAWAPK
>Mature_424_residues
TRKHILSFALMTALGMTVTSTAFAQLHLEIAKAPEQAPKIAIVPFGNDQSIFPIVENDLNRSGRFSSASKNLPTTASIDA
IQASAWQAAGIPYVVVGNLKPAANNAFEVHYQLYDVQKQQYLLNEVLTVPASRVRQAAHMISDAIYQALTGIAGDFSGRI
AYVLRNPATPDQRYTLQIADTDGEQPKTVLSSRDPILSPAWTPDAKKIAYVSFETKRPAIYLQDLATGQREVLASFKGLN
GAPSFSPDGKSMLFTASMNGNPEIYQMDLSTRQLQRMTNDNAIDTEARYAPDGKSFIFTSDRGGSPQIYRYSFDDSSTKR
LTFRGAFNARGTLSADGKNIALVHRPSGSNYKVAIMNISTGIVNILTPTSLDESPSFSPNGQMVVYATYEGSRGLLSIMS
TDGRFRMNLPSEQGEVREPAWAPK

Specific function: Involved in the tonB-independent uptake of proteins

COG id: COG0823

COG function: function code U; Periplasmic component of the Tol biopolymer transport system

Gene ontology:

Cell location: Periplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tolB family

Homologues:

Organism=Escherichia coli, GI1786961, Length=427, Percent_Identity=37.0023419203747, Blast_Score=277, Evalue=1e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TOLB_ACIAD (Q6F985)

Other databases:

- EMBL:   CR543861
- RefSeq:   YP_047202.1
- ProteinModelPortal:   Q6F985
- SMR:   Q6F985
- STRING:   Q6F985
- GeneID:   2880759
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD2622
- NMPDR:   fig|62977.3.peg.2395
- eggNOG:   COG0823
- HOGENOM:   HBG644919
- OMA:   RVADYDG
- PhylomeDB:   Q6F985
- ProtClustDB:   PRK04922
- BioCyc:   ASP62977:ACIAD2622-MONOMER
- HAMAP:   MF_00671
- InterPro:   IPR011659
- InterPro:   IPR014167
- InterPro:   IPR007195
- InterPro:   IPR015943
- Gene3D:   G3DSA:3.40.50.10070
- Gene3D:   G3DSA:2.130.10.10
- TIGRFAMs:   TIGR02800

Pfam domain/function: PF07676 PD40; PF04052 TolB_N; SSF52964 TolB_N

EC number: NA

Molecular weight: Translated: 46429; Mature: 46298

Theoretical pI: Translated: 9.20; Mature: 9.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRKHILSFALMTALGMTVTSTAFAQLHLEIAKAPEQAPKIAIVPFGNDQSIFPIVENDL
CCHHHHHHHHHHHHHCHHHHHHHHHHEEEHHHCCCCCCCCEEEEECCCCCEEEEEECCCC
NRSGRFSSASKNLPTTASIDAIQASAWQAAGIPYVVVGNLKPAANNAFEVHYQLYDVQKQ
CCCCCCCCCCCCCCCCCCCHHHHHCCHHHCCCCEEEEECCCCCCCCEEEEEEEEEHHHHH
QYLLNEVLTVPASRVRQAAHMISDAIYQALTGIAGDFSGRIAYVLRNPATPDQRYTLQIA
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCEEEEEEE
DTDGEQPKTVLSSRDPILSPAWTPDAKKIAYVSFETKRPAIYLQDLATGQREVLASFKGL
CCCCCCCHHHHCCCCCCCCCCCCCCCCEEEEEEEECCCCEEEEEHHCCCHHHHHHHHCCC
NGAPSFSPDGKSMLFTASMNGNPEIYQMDLSTRQLQRMTNDNAIDTEARYAPDGKSFIFT
CCCCCCCCCCCEEEEEEECCCCCCEEEEECCHHHHHHHCCCCCCCCCCEECCCCCEEEEE
SDRGGSPQIYRYSFDDSSTKRLTFRGAFNARGTLSADGKNIALVHRPSGSNYKVAIMNIS
CCCCCCCEEEEEECCCCCCEEEEEEECCCCCCEEECCCCEEEEEECCCCCCEEEEEEEEC
TGIVNILTPTSLDESPSFSPNGQMVVYATYEGSRGLLSIMSTDGRFRMNLPSEQGEVREP
CCEEEEECCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEEECCCEEEECCCCCCCCCCCC
AWAPK
CCCCC
>Mature Secondary Structure 
TRKHILSFALMTALGMTVTSTAFAQLHLEIAKAPEQAPKIAIVPFGNDQSIFPIVENDL
CHHHHHHHHHHHHHCHHHHHHHHHHEEEHHHCCCCCCCCEEEEECCCCCEEEEEECCCC
NRSGRFSSASKNLPTTASIDAIQASAWQAAGIPYVVVGNLKPAANNAFEVHYQLYDVQKQ
CCCCCCCCCCCCCCCCCCCHHHHHCCHHHCCCCEEEEECCCCCCCCEEEEEEEEEHHHHH
QYLLNEVLTVPASRVRQAAHMISDAIYQALTGIAGDFSGRIAYVLRNPATPDQRYTLQIA
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCEEEEEEE
DTDGEQPKTVLSSRDPILSPAWTPDAKKIAYVSFETKRPAIYLQDLATGQREVLASFKGL
CCCCCCCHHHHCCCCCCCCCCCCCCCCEEEEEEEECCCCEEEEEHHCCCHHHHHHHHCCC
NGAPSFSPDGKSMLFTASMNGNPEIYQMDLSTRQLQRMTNDNAIDTEARYAPDGKSFIFT
CCCCCCCCCCCEEEEEEECCCCCCEEEEECCHHHHHHHCCCCCCCCCCEECCCCCEEEEE
SDRGGSPQIYRYSFDDSSTKRLTFRGAFNARGTLSADGKNIALVHRPSGSNYKVAIMNIS
CCCCCCCEEEEEECCCCCCEEEEEEECCCCCCEEECCCCEEEEEECCCCCCEEEEEEEEC
TGIVNILTPTSLDESPSFSPNGQMVVYATYEGSRGLLSIMSTDGRFRMNLPSEQGEVREP
CCEEEEECCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEEECCCEEEECCCCCCCCCCCC
AWAPK
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA