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The map label for this gene is ntaB [H]

Identifier: 50085621

GI number: 50085621

Start: 2504335

End: 2505267

Strand: Reverse

Name: ntaB [H]

Synonym: ACIAD2544

Alternate gene names: 50085621

Gene position: 2505267-2504335 (Counterclockwise)

Preceding gene: 50085622

Following gene: 50085620

Centisome position: 69.62

GC content: 41.69

Gene sequence:

>933_bases
ATGAGCGAAATGGATGCAGTGAATAAAATACGTGAACTACGTGATGCCTTTGGATCATTTATGACAGGCGTGACGGTCGT
GACCACGTGTAAAGATGACGGGACGCCACTTGGTTTCACCGCAAACTCTTTTGCTTCGGTGTCTTTGGACCCAGCTTTGC
TTTTAGTCAGCATTGCCAAGACATCAAGCAATTATCATAACTTTGCAGATGCATCACACTTTGCCATTAATATTTTGGCC
GAAGAACAAAAAGATGTATCCAACATATTTGCTCGTCCAAGTGATGATCGCTTTGCTCAATTGGTCTGGGCAAAAAGTGA
ATATCAAAATCCATTAATTGACGGGGTCAGTGCTTGGTTCGACTGTACAACCTATCAGGTGGTTGATGCAGGTGATCACG
CGATTTTAATTGGTAAGGTTGAAAACTTTACTTCGGCAGGTTTTGCAGGTCTTGGTTATTATCGTGGCGCTTATTTCACG
CCAGCCAAATCCTCTACAGATGTCATTTCAAGCATGAAAGTCATGATGATGGCATTGATTGGGCACGAAAATAAAATCTT
GCTCGAACAGACAGCCGATCATAAATGGGCCTTGCCGCATTTGATGGTCGAAAAAGACGGTGCAGAAAAAGCCTTGGAAA
AGATCTTTGCAACCTATCAACCAGAGGCATCACCCAGCTTTATCTATTCTGTCTATGATGATGTGACGACGCAGCAGCAA
TATATTGCATTTTTATGTAATACCCCTGTTCCAACCGCGCATAAAGGTCAGTTTGTCGATTTAAATGATCTTGAAAAACT
TACTTTTACGGATAGTGCCTTACAAAGCATGTTAATGCGCTATCGCAAAGAAAATTATCTGAAAACCTACGGTGTTTATT
ACGGTAATCATACCAGCGGCACAGTTCGTCAAATTGTTAAAGAGGGAGTTTAA

Upstream 100 bases:

>100_bases
ATATGGTAAATCTCACCGCGCCTGAAAAAGTCAATCAAGCCATGCAAGCGTGGTTGGAAACAACCCCCTAATTTGTTCCA
GAGATTGAAGGAGTATTGAA

Downstream 100 bases:

>100_bases
CCATGCGTTTTTCACTATTTGTACATATGGAACGTGTCTCGGATCAGCAAACCCAGAAACAGCTTTACGATGAAATGATT
GAGCTATGTCAGATTGCAGA

Product: monooxygenase oxydoreductase protein

Products: NAD(+); reduced flavin [C]

Alternate protein names: NTA monooxygenase component B; NTA-MO B [H]

Number of amino acids: Translated: 310; Mature: 309

Protein sequence:

>310_residues
MSEMDAVNKIRELRDAFGSFMTGVTVVTTCKDDGTPLGFTANSFASVSLDPALLLVSIAKTSSNYHNFADASHFAINILA
EEQKDVSNIFARPSDDRFAQLVWAKSEYQNPLIDGVSAWFDCTTYQVVDAGDHAILIGKVENFTSAGFAGLGYYRGAYFT
PAKSSTDVISSMKVMMMALIGHENKILLEQTADHKWALPHLMVEKDGAEKALEKIFATYQPEASPSFIYSVYDDVTTQQQ
YIAFLCNTPVPTAHKGQFVDLNDLEKLTFTDSALQSMLMRYRKENYLKTYGVYYGNHTSGTVRQIVKEGV

Sequences:

>Translated_310_residues
MSEMDAVNKIRELRDAFGSFMTGVTVVTTCKDDGTPLGFTANSFASVSLDPALLLVSIAKTSSNYHNFADASHFAINILA
EEQKDVSNIFARPSDDRFAQLVWAKSEYQNPLIDGVSAWFDCTTYQVVDAGDHAILIGKVENFTSAGFAGLGYYRGAYFT
PAKSSTDVISSMKVMMMALIGHENKILLEQTADHKWALPHLMVEKDGAEKALEKIFATYQPEASPSFIYSVYDDVTTQQQ
YIAFLCNTPVPTAHKGQFVDLNDLEKLTFTDSALQSMLMRYRKENYLKTYGVYYGNHTSGTVRQIVKEGV
>Mature_309_residues
SEMDAVNKIRELRDAFGSFMTGVTVVTTCKDDGTPLGFTANSFASVSLDPALLLVSIAKTSSNYHNFADASHFAINILAE
EQKDVSNIFARPSDDRFAQLVWAKSEYQNPLIDGVSAWFDCTTYQVVDAGDHAILIGKVENFTSAGFAGLGYYRGAYFTP
AKSSTDVISSMKVMMMALIGHENKILLEQTADHKWALPHLMVEKDGAEKALEKIFATYQPEASPSFIYSVYDDVTTQQQY
IAFLCNTPVPTAHKGQFVDLNDLEKLTFTDSALQSMLMRYRKENYLKTYGVYYGNHTSGTVRQIVKEGV

Specific function: Hydroxylation of nitrilotriacetate [H]

COG id: COG1853

COG function: function code R; Conserved protein/domain typically associated with flavoprotein oxygenases, DIM6/NTAB family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87081819, Length=140, Percent_Identity=31.4285714285714, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002563
- InterPro:   IPR012349
- InterPro:   IPR009002
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01613 Flavin_Reduct [H]

EC number: 1.6.8.- [C]

Molecular weight: Translated: 34415; Mature: 34284

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEMDAVNKIRELRDAFGSFMTGVTVVTTCKDDGTPLGFTANSFASVSLDPALLLVSIAK
CCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEECCCCEECCCCHHHHHHHHHH
TSSNYHNFADASHFAINILAEEQKDVSNIFARPSDDRFAQLVWAKSEYQNPLIDGVSAWF
CCCCCCCCCCCCCEEEEEEEHHHHHHHHHHCCCCCCHHHHHEECHHHHCCHHHHHHHHHH
DCTTYQVVDAGDHAILIGKVENFTSAGFAGLGYYRGAYFTPAKSSTDVISSMKVMMMALI
CCCEEEEEECCCCEEEEEECCCCCCCCCCHHHHHCCCEECCCCCCHHHHHHHHHHHHHHH
GHENKILLEQTADHKWALPHLMVEKDGAEKALEKIFATYQPEASPSFIYSVYDDVTTQQQ
CCCCEEEEEECCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCCHHEEHHHHHHHHHHH
YIAFLCNTPVPTAHKGQFVDLNDLEKLTFTDSALQSMLMRYRKENYLKTYGVYYGNHTSG
HHHHEECCCCCCCCCCCEECCCCCHHHEECHHHHHHHHHHHHHHHHHEEEEEEECCCCCH
TVRQIVKEGV
HHHHHHHHCC
>Mature Secondary Structure 
SEMDAVNKIRELRDAFGSFMTGVTVVTTCKDDGTPLGFTANSFASVSLDPALLLVSIAK
CHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEECCCCEECCCCHHHHHHHHHH
TSSNYHNFADASHFAINILAEEQKDVSNIFARPSDDRFAQLVWAKSEYQNPLIDGVSAWF
CCCCCCCCCCCCCEEEEEEEHHHHHHHHHHCCCCCCHHHHHEECHHHHCCHHHHHHHHHH
DCTTYQVVDAGDHAILIGKVENFTSAGFAGLGYYRGAYFTPAKSSTDVISSMKVMMMALI
CCCEEEEEECCCCEEEEEECCCCCCCCCCHHHHHCCCEECCCCCCHHHHHHHHHHHHHHH
GHENKILLEQTADHKWALPHLMVEKDGAEKALEKIFATYQPEASPSFIYSVYDDVTTQQQ
CCCCEEEEEECCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCCHHEEHHHHHHHHHHH
YIAFLCNTPVPTAHKGQFVDLNDLEKLTFTDSALQSMLMRYRKENYLKTYGVYYGNHTSG
HHHHEECCCCCCCCCCCEECCCCCHHHEECHHHHHHHHHHHHHHHHHEEEEEEECCCCCH
TVRQIVKEGV
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; flavin [C]

Specific reaction: NADH + flavin = NAD(+) + reduced flavin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8892809; 9023192; 1735711 [H]