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The map label for this gene is glnT [H]

Identifier: 50085607

GI number: 50085607

Start: 2485926

End: 2487317

Strand: Reverse

Name: glnT [H]

Synonym: ACIAD2528

Alternate gene names: 50085607

Gene position: 2487317-2485926 (Counterclockwise)

Preceding gene: 50085609

Following gene: 50085606

Centisome position: 69.12

GC content: 45.76

Gene sequence:

>1392_bases
ATGTTGCAGCAAGCCGTGAAGGAGATGTTTGATCATCATGAATTGACCAGTCAACCCATGTACCGACCAGAAGTACAAAA
GGTCATTGATCAGCATCATTTAAAATATGTTTTGGCGCAATTTGTTGATATTCATGGTGCTGCCAAAACCAAGTCGGTTC
CCATTTCAGGTTTGAAAGCGATTGAAAAAGACGGTGTTGGATTTGCAGGTTTTGCTATTTCTGGTATGGGAATGCAACCC
CATGGTCCTGACTTTATGGTGAAAGGCGATTTATCCACTCTAACTCCTGTGCCTTGGCAACCGGGTTACGGACGTGTAGT
TTGTGAAGGCTATGTTAACGGCAAGCCGCATCCTTACGATTCACGCTATGTATTACGTCAGCAAGTAGAACGTTTGAGTG
CGCAAGGCTGGACCTTAAATACTGGGCTTGAGCCAGAGTTTAGCCTGTTTCGCCGTAGCGAAGATGGCAAACTGAAAATG
GTCGATGACTCCGATAATCTGGATAAACCTTGTTATGACTATAAAGGCTTGTCTCGTTCACGTGAGTTTTTAGAGCGTTT
AACCGAAGCTCTCATTCCAGTCGGGTTTGATATTTACCAGATTGATCATGAAGATGCCAATGGTCAGTTTGAGATCAATT
ATACCTATGCAGACGCCATGAAGTCGGCAGATATGTTTACCTTTTTCCGTATGGCTGCGGGTGAAATCGCCAATGATTTG
GGCATGATCTGTTCATTTATGCCAAAACCTGATCCAAAACGTGCGGGCAATGGTATGCACTTCCACCTATCAATTGGCAG
TGATGAATGCAGCAACCTGTTCCATGATGCCAGTGATCCAAGTGGGATGGGCTTATCCAAAATGGCCTATCACTTTATTG
CAGGTTTACTTGAGCATGGTCCAGCACTCTGTGCTTTTTGTGCGCCCACGGTAAATTCTTATAAACGCCTTGTGGTGGGC
CGTTCACTCTCTGGCGCGACATGGGCACCTGCGTTCATTGCTTATGGTGGCAACAACCGTTCAGCCATGGTGCGTATTCC
ATATGGTCGTATCGAGCTGCGCTTACCCGATGCAGGCTGTAATCCTTATCTGGTGTCGGCTGCCATTATTGCAGCAGGGC
TTGACGGAATCGAACGTGAATTGATGCCACCAACTGCATGCAATGAAAACCTCTATAGCCTTGGGCTTGAAGAGATTGCA
GCACGCGGCATTCAAACCTTACCGCAGTCACTCAAAGAAGCCATTGATGCACTTAAGGCAGATCCATTGTTTGCACAGAA
AATGGGATCTGAAATTGTGAATGAGTTTATTGAACAAAAAAGCATGGAGTGGGTGGAATACTGCCGTCATGTGTCCGACT
GGGAAATTCAGCGTTATACCGAGTTTTTCTAA

Upstream 100 bases:

>100_bases
ATAGGAAATAAAGTTTCTTATACTGAAAGATGAATCATGTAAGACCACGATGCATTTTTCAAGCCAAATACAGTTTTGCC
ATATTAGGAGTATGTCAATG

Downstream 100 bases:

>100_bases
AGCATTAAACAACAGACAGATTAAAAATTAAATTTTGGAGACATCCTATGTGTGGAATCGTTGGTTTATATCTTAAAAAT
CCTGCGCTCGAATCTCAGCT

Product: glutamine synthetase

Products: NA

Alternate protein names: Glutamate--ammonia ligase; Glutamine synthetase III; GSIII [H]

Number of amino acids: Translated: 463; Mature: 463

Protein sequence:

>463_residues
MLQQAVKEMFDHHELTSQPMYRPEVQKVIDQHHLKYVLAQFVDIHGAAKTKSVPISGLKAIEKDGVGFAGFAISGMGMQP
HGPDFMVKGDLSTLTPVPWQPGYGRVVCEGYVNGKPHPYDSRYVLRQQVERLSAQGWTLNTGLEPEFSLFRRSEDGKLKM
VDDSDNLDKPCYDYKGLSRSREFLERLTEALIPVGFDIYQIDHEDANGQFEINYTYADAMKSADMFTFFRMAAGEIANDL
GMICSFMPKPDPKRAGNGMHFHLSIGSDECSNLFHDASDPSGMGLSKMAYHFIAGLLEHGPALCAFCAPTVNSYKRLVVG
RSLSGATWAPAFIAYGGNNRSAMVRIPYGRIELRLPDAGCNPYLVSAAIIAAGLDGIERELMPPTACNENLYSLGLEEIA
ARGIQTLPQSLKEAIDALKADPLFAQKMGSEIVNEFIEQKSMEWVEYCRHVSDWEIQRYTEFF

Sequences:

>Translated_463_residues
MLQQAVKEMFDHHELTSQPMYRPEVQKVIDQHHLKYVLAQFVDIHGAAKTKSVPISGLKAIEKDGVGFAGFAISGMGMQP
HGPDFMVKGDLSTLTPVPWQPGYGRVVCEGYVNGKPHPYDSRYVLRQQVERLSAQGWTLNTGLEPEFSLFRRSEDGKLKM
VDDSDNLDKPCYDYKGLSRSREFLERLTEALIPVGFDIYQIDHEDANGQFEINYTYADAMKSADMFTFFRMAAGEIANDL
GMICSFMPKPDPKRAGNGMHFHLSIGSDECSNLFHDASDPSGMGLSKMAYHFIAGLLEHGPALCAFCAPTVNSYKRLVVG
RSLSGATWAPAFIAYGGNNRSAMVRIPYGRIELRLPDAGCNPYLVSAAIIAAGLDGIERELMPPTACNENLYSLGLEEIA
ARGIQTLPQSLKEAIDALKADPLFAQKMGSEIVNEFIEQKSMEWVEYCRHVSDWEIQRYTEFF
>Mature_463_residues
MLQQAVKEMFDHHELTSQPMYRPEVQKVIDQHHLKYVLAQFVDIHGAAKTKSVPISGLKAIEKDGVGFAGFAISGMGMQP
HGPDFMVKGDLSTLTPVPWQPGYGRVVCEGYVNGKPHPYDSRYVLRQQVERLSAQGWTLNTGLEPEFSLFRRSEDGKLKM
VDDSDNLDKPCYDYKGLSRSREFLERLTEALIPVGFDIYQIDHEDANGQFEINYTYADAMKSADMFTFFRMAAGEIANDL
GMICSFMPKPDPKRAGNGMHFHLSIGSDECSNLFHDASDPSGMGLSKMAYHFIAGLLEHGPALCAFCAPTVNSYKRLVVG
RSLSGATWAPAFIAYGGNNRSAMVRIPYGRIELRLPDAGCNPYLVSAAIIAAGLDGIERELMPPTACNENLYSLGLEEIA
ARGIQTLPQSLKEAIDALKADPLFAQKMGSEIVNEFIEQKSMEWVEYCRHVSDWEIQRYTEFF

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family. Type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=443, Percent_Identity=24.8306997742664, Blast_Score=122, Evalue=6e-28,
Organism=Escherichia coli, GI1790301, Length=479, Percent_Identity=29.8538622129436, Blast_Score=159, Evalue=3e-40,
Organism=Escherichia coli, GI87081870, Length=467, Percent_Identity=27.4089935760171, Blast_Score=137, Evalue=2e-33,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR017536 [H]

Pfam domain/function: PF00120 Gln-synt_C [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 51614; Mature: 51614

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQQAVKEMFDHHELTSQPMYRPEVQKVIDQHHLKYVLAQFVDIHGAAKTKSVPISGLKA
CHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
IEKDGVGFAGFAISGMGMQPHGPDFMVKGDLSTLTPVPWQPGYGRVVCEGYVNGKPHPYD
HHHCCCCEEHHEEECCCCCCCCCCEEEECCCCCCCCCCCCCCCCCEEEEEECCCCCCCCC
SRYVLRQQVERLSAQGWTLNTGLEPEFSLFRRSEDGKLKMVDDSDNLDKPCYDYKGLSRS
HHHHHHHHHHHHHCCCCEECCCCCCCHHHHHCCCCCCEEEECCCCCCCCCCHHHCCCHHH
REFLERLTEALIPVGFDIYQIDHEDANGQFEINYTYADAMKSADMFTFFRMAAGEIANDL
HHHHHHHHHHHHHCCCEEEEECCCCCCCEEEEEEEHHHHHHCCHHHHHHHHHHHHHHHHH
GMICSFMPKPDPKRAGNGMHFHLSIGSDECSNLFHDASDPSGMGLSKMAYHFIAGLLEHG
HHHHHCCCCCCCCCCCCCEEEEEEECCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCC
PALCAFCAPTVNSYKRLVVGRSLSGATWAPAFIAYGGNNRSAMVRIPYGRIELRLPDAGC
CCEEHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCEEEEEECCCEEEEECCCCCC
NPYLVSAAIIAAGLDGIERELMPPTACNENLYSLGLEEIAARGIQTLPQSLKEAIDALKA
CHHHHHHHHHHHCCHHHHHHCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCC
DPLFAQKMGSEIVNEFIEQKSMEWVEYCRHVSDWEIQRYTEFF
CCHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCHHHHHHHCC
>Mature Secondary Structure
MLQQAVKEMFDHHELTSQPMYRPEVQKVIDQHHLKYVLAQFVDIHGAAKTKSVPISGLKA
CHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
IEKDGVGFAGFAISGMGMQPHGPDFMVKGDLSTLTPVPWQPGYGRVVCEGYVNGKPHPYD
HHHCCCCEEHHEEECCCCCCCCCCEEEECCCCCCCCCCCCCCCCCEEEEEECCCCCCCCC
SRYVLRQQVERLSAQGWTLNTGLEPEFSLFRRSEDGKLKMVDDSDNLDKPCYDYKGLSRS
HHHHHHHHHHHHHCCCCEECCCCCCCHHHHHCCCCCCEEEECCCCCCCCCCHHHCCCHHH
REFLERLTEALIPVGFDIYQIDHEDANGQFEINYTYADAMKSADMFTFFRMAAGEIANDL
HHHHHHHHHHHHHCCCEEEEECCCCCCCEEEEEEEHHHHHHCCHHHHHHHHHHHHHHHHH
GMICSFMPKPDPKRAGNGMHFHLSIGSDECSNLFHDASDPSGMGLSKMAYHFIAGLLEHG
HHHHHCCCCCCCCCCCCCEEEEEEECCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCC
PALCAFCAPTVNSYKRLVVGRSLSGATWAPAFIAYGGNNRSAMVRIPYGRIELRLPDAGC
CCEEHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCEEEEEECCCEEEEECCCCCC
NPYLVSAAIIAAGLDGIERELMPPTACNENLYSLGLEEIAARGIQTLPQSLKEAIDALKA
CHHHHHHHHHHHCCHHHHHHCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCC
DPLFAQKMGSEIVNEFIEQKSMEWVEYCRHVSDWEIQRYTEFF
CCHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1356885 [H]