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The map label for this gene is folE2 [H]

Identifier: 50085551

GI number: 50085551

Start: 2432069

End: 2432662

Strand: Direct

Name: folE2 [H]

Synonym: ACIAD2470

Alternate gene names: 50085551

Gene position: 2432069-2432662 (Clockwise)

Preceding gene: 50085547

Following gene: 50085552

Centisome position: 67.58

GC content: 40.74

Gene sequence:

>594_bases
ATGCCGACCTTATTTAATCGAGTTAAGTCCAAACGTCTTATGTCAATGCAGCAATCTTACGCAAATATCCTGACCGCAGT
GGGCGAGGATTTAAACCGTCCGGGGCTAAAAGATACGCCTATTCGCGCTGCCAAGGCATTTTCGTTTTTAACCTCTGGCT
ATGGAAAAAGTCTGGAAGAAGTGACGAATAATGCAGTCTTCCCTTCAGACAACCATGAAATGGTTCTCGTTAAAAATATT
GAATTTTATTCTTTATGTGAACATCATTTACTTCCATTTTATGGACGTGTACATATTGCTTATCTTCCAGAAGGTCAAGT
TTTAGGCTTATCAAAGTTTGCACGTATTACAGAAATGTTTGCGCGACGTTTGCAAATTCAGGAAAATCTGACTCAGCAAA
TTGCACAAGCAGTTGAAGAAGTAACCAAAGCGCGTGGTGTGGCAGTCATGATTGATTCAGCACATATGTGCATGATGATG
CGCGGTGTAGGCAAACAAGAATCAACTACACGTACTGTATCGTTCATTGGCGACTTTAAAACCGATAAAGAAGCGCGCCG
TGAATTTTTAAGTGCAGTACCAGAAAGCTATTAA

Upstream 100 bases:

>100_bases
TTTTTTAAAGATGAAAATTATTCAGTTTTTATTTAATTCGCCCAGAGGGTTGTAAATATAGCTCGACAGCCTATGAACAT
CAGACAATGTTTCTGGTACA

Downstream 100 bases:

>100_bases
TTCCTTGACATCATTTTAGTTAAGATGTTGTCTATCTTGGTGAGTCATATCTTTAGTTTATGCATTAACGAAGAGGTAAA
AACGAGATGAAGGTAGATCG

Product: GTP cyclohydrolase I

Products: NA

Alternate protein names: GTP cyclohydrolase I; GTP-CH-I [H]

Number of amino acids: Translated: 197; Mature: 196

Protein sequence:

>197_residues
MPTLFNRVKSKRLMSMQQSYANILTAVGEDLNRPGLKDTPIRAAKAFSFLTSGYGKSLEEVTNNAVFPSDNHEMVLVKNI
EFYSLCEHHLLPFYGRVHIAYLPEGQVLGLSKFARITEMFARRLQIQENLTQQIAQAVEEVTKARGVAVMIDSAHMCMMM
RGVGKQESTTRTVSFIGDFKTDKEARREFLSAVPESY

Sequences:

>Translated_197_residues
MPTLFNRVKSKRLMSMQQSYANILTAVGEDLNRPGLKDTPIRAAKAFSFLTSGYGKSLEEVTNNAVFPSDNHEMVLVKNI
EFYSLCEHHLLPFYGRVHIAYLPEGQVLGLSKFARITEMFARRLQIQENLTQQIAQAVEEVTKARGVAVMIDSAHMCMMM
RGVGKQESTTRTVSFIGDFKTDKEARREFLSAVPESY
>Mature_196_residues
PTLFNRVKSKRLMSMQQSYANILTAVGEDLNRPGLKDTPIRAAKAFSFLTSGYGKSLEEVTNNAVFPSDNHEMVLVKNIE
FYSLCEHHLLPFYGRVHIAYLPEGQVLGLSKFARITEMFARRLQIQENLTQQIAQAVEEVTKARGVAVMIDSAHMCMMMR
GVGKQESTTRTVSFIGDFKTDKEARREFLSAVPESY

Specific function: Tetrahydrofolate biosynthesis; first step. [C]

COG id: COG0302

COG function: function code H; GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family [H]

Homologues:

Organism=Homo sapiens, GI66932968, Length=181, Percent_Identity=50.2762430939227, Blast_Score=203, Evalue=6e-53,
Organism=Homo sapiens, GI4503949, Length=181, Percent_Identity=50.2762430939227, Blast_Score=203, Evalue=6e-53,
Organism=Homo sapiens, GI66932972, Length=141, Percent_Identity=51.063829787234, Blast_Score=163, Evalue=9e-41,
Organism=Homo sapiens, GI66932970, Length=151, Percent_Identity=48.3443708609272, Blast_Score=163, Evalue=9e-41,
Organism=Escherichia coli, GI1788476, Length=176, Percent_Identity=38.6363636363636, Blast_Score=106, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17560486, Length=185, Percent_Identity=54.0540540540541, Blast_Score=212, Evalue=1e-55,
Organism=Saccharomyces cerevisiae, GI6321706, Length=181, Percent_Identity=51.3812154696133, Blast_Score=197, Evalue=9e-52,
Organism=Drosophila melanogaster, GI24656796, Length=181, Percent_Identity=54.1436464088398, Blast_Score=192, Evalue=2e-49,
Organism=Drosophila melanogaster, GI24656791, Length=181, Percent_Identity=54.1436464088398, Blast_Score=192, Evalue=2e-49,
Organism=Drosophila melanogaster, GI24656782, Length=181, Percent_Identity=54.1436464088398, Blast_Score=192, Evalue=2e-49,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001474
- InterPro:   IPR020602
- InterPro:   IPR018234 [H]

Pfam domain/function: PF01227 GTP_cyclohydroI [H]

EC number: =3.5.4.16 [H]

Molecular weight: Translated: 22260; Mature: 22129

Theoretical pI: Translated: 9.06; Mature: 9.06

Prosite motif: PS00859 GTP_CYCLOHYDROL_1_1 ; PS00860 GTP_CYCLOHYDROL_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTLFNRVKSKRLMSMQQSYANILTAVGEDLNRPGLKDTPIRAAKAFSFLTSGYGKSLEE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCHHHHH
VTNNAVFPSDNHEMVLVKNIEFYSLCEHHLLPFYGRVHIAYLPEGQVLGLSKFARITEMF
HHCCCCCCCCCCCEEEEECCHHHHHHHHHCCCHHCCEEEEECCCCCEECHHHHHHHHHHH
ARRLQIQENLTQQIAQAVEEVTKARGVAVMIDSAHMCMMMRGVGKQESTTRTVSFIGDFK
HHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHCCCCCCCHHHHHHHHHCCC
TDKEARREFLSAVPESY
CCHHHHHHHHHHCCCCC
>Mature Secondary Structure 
PTLFNRVKSKRLMSMQQSYANILTAVGEDLNRPGLKDTPIRAAKAFSFLTSGYGKSLEE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCHHHHH
VTNNAVFPSDNHEMVLVKNIEFYSLCEHHLLPFYGRVHIAYLPEGQVLGLSKFARITEMF
HHCCCCCCCCCCCEEEEECCHHHHHHHHHCCCHHCCEEEEECCCCCEECHHHHHHHHHHH
ARRLQIQENLTQQIAQAVEEVTKARGVAVMIDSAHMCMMMRGVGKQESTTRTVSFIGDFK
HHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHCCCCCCCHHHHHHHHHCCC
TDKEARREFLSAVPESY
CCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA