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The map label for this gene is ppsA [H]

Identifier: 50085508

GI number: 50085508

Start: 2387954

End: 2390332

Strand: Direct

Name: ppsA [H]

Synonym: ACIAD2423

Alternate gene names: 50085508

Gene position: 2387954-2390332 (Clockwise)

Preceding gene: 50085505

Following gene: 50085509

Centisome position: 66.36

GC content: 42.58

Gene sequence:

>2379_bases
TTGGAAGCGCGCGTAATCGGTCTAGAAAAATTAGGGAAGCACGATGTCGAACTCGTAGGTGGGAAAAACTCATCTTTGGG
TGAAATGATCAGCCATTTATCCAATGCTGGTGTATCTGTACCTGGTGGTTTTGCGACAACTGCAGCTGCTTATCGTGAAT
TCCTCGATCAAAGTGGACTCAACGCTAAAATTCAAGCAGAACTTGCAAAACTCAATGTAGAAGACGTGAATGCATTGGCT
GAATCAGGCGCAAAAATTCGTCAATGGATTATCGAAACTCCTCTTACTCCAGAATTTGAAAAAGAAGTACGCGAAGCTTT
TAACGCACTTTCTAACGGCAACCCTGAAATCGCGGTTGCCGTTCGTTCATCTGCGACTGCAGAAGATCTACCTGATGCTT
CTTTTGCAGGTCAACAAGAAACCTTCCTCAACATTCGTGGAATTGACAATGTGTTAATCGCGATTAAGGAAGTTTTTGCA
TCATTGTATAATGACCGTGCAATCGCTTATCGTGTTCACCAAGGTTTCGCACATGACGTTGTTGCGCTTTCAGCGGGTGT
GCAACGCATGGTGCGCTCTGAAACAGGTGCTGCAGGCGTAATGTTTACACTCGACACCGAATCTGGTTTCCGTGATGTTG
TGTTTATTACTGCATCTTATGGTCTTGGTGAAATGGTGGTACAAGGTGCTGTCAACCCTGATGAATTTTATTTATCAAAA
CCATTACTTAAAAATGGTAAACACTCTGTATTACGTCGCAATCTTGGCTCTAAACATCAGAAAATGATTTATGGGGAAGA
AGGTTCAGCAGGTAAGTCAGTTGTTGTAGTTGATGTAGAAAAACAAGACCGTCAACAATTTGCACTCAATGATCACGAAT
TACAAGAACTCGCTAAACAAGCCATGATCATTGAAGACCATTATGGTGCCCCAATGGACATCGAGTGGGCAAAAGATGGT
GATGATGGTCAAATTTATATCGTTCAGGCACGTCCTGAAACCGTAAAAAGCCGTCAAAACGTTGGCACAATGGAGCGTTA
CCTACTTAAGCAACGTGGTACAGTCGTTTGTGAAGGTCGCTCAATCGGTCAACGTATCGGTTCTGGTAAAGTTCGTATTG
TGAGTTCTATCAAGGAAATGGATAAAGTCCAAGACGGTGATGTTCTCGTTTCAGATATGACAGATCCAGATTGGGAACCA
GTGATGAAACGTGCGGCAGCGATTGTCACCAACCGCGGTGGTCGTACTTGCCACGCTGCAATTATTGCGCGTGAACTTGG
CGTACCTGCTATTGTTGGTTGCGGCAATGCAACTGAAGTGTTAACTGATGGACAAGAAGTGACGGTTTCTTGTGCAGAAG
GTGATACTGGCTTTATTTATGAAGGTTCACTTGATTTTGAAGTACAACGCAACTCTATTGAGTCTATGCCTAAACTTCCG
TTCAAGATCATGATGAATGTCGGTAATCCAGACCGCGCATTTGACTTTGCTCAAATTCCAAATGAAGGTATTGGTCTTGC
ACGTCTTGAGTTCATTATCAACCGCATGATTGGCGTACATCCTAAAGCATTGTTAAACATTGATAGCTTACCACGTGAAA
CTCGTGCTGCTGTTCTTGCCCGTACTGCTGGATATACCTCTCCTATCGATTTTTATGTTGAAAAGCTAGTTGAAGGCATT
TCAACATTGGCGGCAGCTTTTGCTGAGAAGCCAGTGATTGTTCGTATGTCAGACTTTAAATCAAATGAGTATGCCAATCT
GATTGGCGGAAAATTATATGAGCCTGAAGAAGAAAATCCAATGCTTGGCTTCCGTGGTGCAAGCCGTTATGTATCTGATA
ACTTCCGTGACTGTTTTGAGCTTGAATGTCGTGCCTTGAAAAAAGCACGTGATGAAATGGGCTTGACCAATATTCAAATC
ATGATTCCATTCGTACGTACTGTTGCAGAAGCGAAACGTGTAATTGAATTACTTGCTCTGAATGGCCTTAAACGTGGCGA
AAATGGCTTAAAAGTAATCATGATGTGCGAACTTCCAACTAACGCACTATTGGCTGAGCAATTCCTTGAACATTTCGATG
GTTTCTCAATCGGTTCAAATGATTTAACTCAGTTAACTTTAGGCTTAGATCGTGACTCTGGTATCGTATCTCATTTATTT
GATGAACGCGATCCAGCAGTAAAAGCATTACTTTCAATGGCGATTCATGCTTGCCGTAAAGCTGGAAAATATGTGGGTAT
CTGTGGCCAAGGCCCATCAGATCATCCAGATCTAGCAAAATGGTTAATGGAGCAAGGTATTGAATCTGTTTCACTTAATC
CAGATTCGGTGTTAGATACTTGGTTCTTCCTTGCCGAAGAGAAAACAAAGCAAGCCTGA

Upstream 100 bases:

>100_bases
TTACATGACTGTCGCTTAGTAAAAATCAAAAATCTAAGCAAATTAAATCATGTCTTCATGCCAAGATAGTAATTAATACT
GGAAATGTGGAGTAAAAACT

Downstream 100 bases:

>100_bases
CTTTTATAAGAAAAAGACCCACCACACGTGGGTCTTTTTTCATCATTAGATGTTGAGATTGAAGACTTATGCAAATTTAC
CTGGCACGCAATAATCAACA

Product: phosphoenolpyruvate synthase

Products: NA

Alternate protein names: PEP synthase; Pyruvate, water dikinase [H]

Number of amino acids: Translated: 792; Mature: 792

Protein sequence:

>792_residues
MEARVIGLEKLGKHDVELVGGKNSSLGEMISHLSNAGVSVPGGFATTAAAYREFLDQSGLNAKIQAELAKLNVEDVNALA
ESGAKIRQWIIETPLTPEFEKEVREAFNALSNGNPEIAVAVRSSATAEDLPDASFAGQQETFLNIRGIDNVLIAIKEVFA
SLYNDRAIAYRVHQGFAHDVVALSAGVQRMVRSETGAAGVMFTLDTESGFRDVVFITASYGLGEMVVQGAVNPDEFYLSK
PLLKNGKHSVLRRNLGSKHQKMIYGEEGSAGKSVVVVDVEKQDRQQFALNDHELQELAKQAMIIEDHYGAPMDIEWAKDG
DDGQIYIVQARPETVKSRQNVGTMERYLLKQRGTVVCEGRSIGQRIGSGKVRIVSSIKEMDKVQDGDVLVSDMTDPDWEP
VMKRAAAIVTNRGGRTCHAAIIARELGVPAIVGCGNATEVLTDGQEVTVSCAEGDTGFIYEGSLDFEVQRNSIESMPKLP
FKIMMNVGNPDRAFDFAQIPNEGIGLARLEFIINRMIGVHPKALLNIDSLPRETRAAVLARTAGYTSPIDFYVEKLVEGI
STLAAAFAEKPVIVRMSDFKSNEYANLIGGKLYEPEEENPMLGFRGASRYVSDNFRDCFELECRALKKARDEMGLTNIQI
MIPFVRTVAEAKRVIELLALNGLKRGENGLKVIMMCELPTNALLAEQFLEHFDGFSIGSNDLTQLTLGLDRDSGIVSHLF
DERDPAVKALLSMAIHACRKAGKYVGICGQGPSDHPDLAKWLMEQGIESVSLNPDSVLDTWFFLAEEKTKQA

Sequences:

>Translated_792_residues
MEARVIGLEKLGKHDVELVGGKNSSLGEMISHLSNAGVSVPGGFATTAAAYREFLDQSGLNAKIQAELAKLNVEDVNALA
ESGAKIRQWIIETPLTPEFEKEVREAFNALSNGNPEIAVAVRSSATAEDLPDASFAGQQETFLNIRGIDNVLIAIKEVFA
SLYNDRAIAYRVHQGFAHDVVALSAGVQRMVRSETGAAGVMFTLDTESGFRDVVFITASYGLGEMVVQGAVNPDEFYLSK
PLLKNGKHSVLRRNLGSKHQKMIYGEEGSAGKSVVVVDVEKQDRQQFALNDHELQELAKQAMIIEDHYGAPMDIEWAKDG
DDGQIYIVQARPETVKSRQNVGTMERYLLKQRGTVVCEGRSIGQRIGSGKVRIVSSIKEMDKVQDGDVLVSDMTDPDWEP
VMKRAAAIVTNRGGRTCHAAIIARELGVPAIVGCGNATEVLTDGQEVTVSCAEGDTGFIYEGSLDFEVQRNSIESMPKLP
FKIMMNVGNPDRAFDFAQIPNEGIGLARLEFIINRMIGVHPKALLNIDSLPRETRAAVLARTAGYTSPIDFYVEKLVEGI
STLAAAFAEKPVIVRMSDFKSNEYANLIGGKLYEPEEENPMLGFRGASRYVSDNFRDCFELECRALKKARDEMGLTNIQI
MIPFVRTVAEAKRVIELLALNGLKRGENGLKVIMMCELPTNALLAEQFLEHFDGFSIGSNDLTQLTLGLDRDSGIVSHLF
DERDPAVKALLSMAIHACRKAGKYVGICGQGPSDHPDLAKWLMEQGIESVSLNPDSVLDTWFFLAEEKTKQA
>Mature_792_residues
MEARVIGLEKLGKHDVELVGGKNSSLGEMISHLSNAGVSVPGGFATTAAAYREFLDQSGLNAKIQAELAKLNVEDVNALA
ESGAKIRQWIIETPLTPEFEKEVREAFNALSNGNPEIAVAVRSSATAEDLPDASFAGQQETFLNIRGIDNVLIAIKEVFA
SLYNDRAIAYRVHQGFAHDVVALSAGVQRMVRSETGAAGVMFTLDTESGFRDVVFITASYGLGEMVVQGAVNPDEFYLSK
PLLKNGKHSVLRRNLGSKHQKMIYGEEGSAGKSVVVVDVEKQDRQQFALNDHELQELAKQAMIIEDHYGAPMDIEWAKDG
DDGQIYIVQARPETVKSRQNVGTMERYLLKQRGTVVCEGRSIGQRIGSGKVRIVSSIKEMDKVQDGDVLVSDMTDPDWEP
VMKRAAAIVTNRGGRTCHAAIIARELGVPAIVGCGNATEVLTDGQEVTVSCAEGDTGFIYEGSLDFEVQRNSIESMPKLP
FKIMMNVGNPDRAFDFAQIPNEGIGLARLEFIINRMIGVHPKALLNIDSLPRETRAAVLARTAGYTSPIDFYVEKLVEGI
STLAAAFAEKPVIVRMSDFKSNEYANLIGGKLYEPEEENPMLGFRGASRYVSDNFRDCFELECRALKKARDEMGLTNIQI
MIPFVRTVAEAKRVIELLALNGLKRGENGLKVIMMCELPTNALLAEQFLEHFDGFSIGSNDLTQLTLGLDRDSGIVSHLF
DERDPAVKALLSMAIHACRKAGKYVGICGQGPSDHPDLAKWLMEQGIESVSLNPDSVLDTWFFLAEEKTKQA

Specific function: Catalyzes the phosphorylation of pyruvate to phosphoenolpyruvate [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1787994, Length=784, Percent_Identity=66.4540816326531, Blast_Score=1110, Evalue=0.0,
Organism=Escherichia coli, GI1788756, Length=459, Percent_Identity=26.5795206971678, Blast_Score=125, Evalue=8e-30,
Organism=Escherichia coli, GI1788726, Length=382, Percent_Identity=25.130890052356, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI48994992, Length=190, Percent_Identity=32.1052631578947, Blast_Score=92, Evalue=2e-19,
Organism=Escherichia coli, GI1789193, Length=417, Percent_Identity=24.9400479616307, Blast_Score=88, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17564524, Length=329, Percent_Identity=26.7477203647416, Blast_Score=92, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR006319
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.2 [H]

Molecular weight: Translated: 86715; Mature: 86715

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEARVIGLEKLGKHDVELVGGKNSSLGEMISHLSNAGVSVPGGFATTAAAYREFLDQSGL
CCCEEECHHHCCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCC
NAKIQAELAKLNVEDVNALAESGAKIRQWIIETPLTPEFEKEVREAFNALSNGNPEIAVA
CCEEEHHHHHCCHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCEEEEE
VRSSATAEDLPDASFAGQQETFLNIRGIDNVLIAIKEVFASLYNDRAIAYRVHQGFAHDV
EECCCCHHCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHH
VALSAGVQRMVRSETGAAGVMFTLDTESGFRDVVFITASYGLGEMVVQGAVNPDEFYLSK
HHHHHHHHHHHHHCCCCCEEEEEEECCCCCCEEEEEEECCCCHHHHHHCCCCHHHHHHCC
PLLKNGKHSVLRRNLGSKHQKMIYGEEGSAGKSVVVVDVEKQDRQQFALNDHELQELAKQ
HHHHCCHHHHHHHHCCCHHHEEEECCCCCCCCEEEEEECCCCCHHHHCCCHHHHHHHHHH
AMIIEDHYGAPMDIEWAKDGDDGQIYIVQARPETVKSRQNVGTMERYLLKQRGTVVCEGR
HEEEECCCCCCEEEEECCCCCCCEEEEEECCCHHHHHHHCCCHHHHHHHHHCCCEEECCC
SIGQRIGSGKVRIVSSIKEMDKVQDGDVLVSDMTDPDWEPVMKRAAAIVTNRGGRTCHAA
HHHHHCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCHHHH
IIARELGVPAIVGCGNATEVLTDGQEVTVSCAEGDTGFIYEGSLDFEVQRNSIESMPKLP
HHHHHCCCCEEEECCCCHHHHCCCCEEEEEEECCCCCEEEECCCCEEEECCHHHCCCCCC
FKIMMNVGNPDRAFDFAQIPNEGIGLARLEFIINRMIGVHPKALLNIDSLPRETRAAVLA
EEEEEECCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHCCCCHHHCCCCCCCHHHHHHHHH
RTAGYTSPIDFYVEKLVEGISTLAAAFAEKPVIVRMSDFKSNEYANLIGGKLYEPEEENP
HHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHCCCCCCCCCCCC
MLGFRGASRYVSDNFRDCFELECRALKKARDEMGLTNIQIMIPFVRTVAEAKRVIELLAL
CEEECCCCHHHHCCHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHH
NGLKRGENGLKVIMMCELPTNALLAEQFLEHFDGFSIGSNDLTQLTLGLDRDSGIVSHLF
CCCCCCCCCCEEEEEEECCCHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCHHHHHH
DERDPAVKALLSMAIHACRKAGKYVGICGQGPSDHPDLAKWLMEQGIESVSLNPDSVLDT
CCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHCCCCEECCCCHHHHHH
WFFLAEEKTKQA
HHHHHHHHHCCC
>Mature Secondary Structure
MEARVIGLEKLGKHDVELVGGKNSSLGEMISHLSNAGVSVPGGFATTAAAYREFLDQSGL
CCCEEECHHHCCCCCEEEECCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCC
NAKIQAELAKLNVEDVNALAESGAKIRQWIIETPLTPEFEKEVREAFNALSNGNPEIAVA
CCEEEHHHHHCCHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCEEEEE
VRSSATAEDLPDASFAGQQETFLNIRGIDNVLIAIKEVFASLYNDRAIAYRVHQGFAHDV
EECCCCHHCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHH
VALSAGVQRMVRSETGAAGVMFTLDTESGFRDVVFITASYGLGEMVVQGAVNPDEFYLSK
HHHHHHHHHHHHHCCCCCEEEEEEECCCCCCEEEEEEECCCCHHHHHHCCCCHHHHHHCC
PLLKNGKHSVLRRNLGSKHQKMIYGEEGSAGKSVVVVDVEKQDRQQFALNDHELQELAKQ
HHHHCCHHHHHHHHCCCHHHEEEECCCCCCCCEEEEEECCCCCHHHHCCCHHHHHHHHHH
AMIIEDHYGAPMDIEWAKDGDDGQIYIVQARPETVKSRQNVGTMERYLLKQRGTVVCEGR
HEEEECCCCCCEEEEECCCCCCCEEEEEECCCHHHHHHHCCCHHHHHHHHHCCCEEECCC
SIGQRIGSGKVRIVSSIKEMDKVQDGDVLVSDMTDPDWEPVMKRAAAIVTNRGGRTCHAA
HHHHHCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCHHHH
IIARELGVPAIVGCGNATEVLTDGQEVTVSCAEGDTGFIYEGSLDFEVQRNSIESMPKLP
HHHHHCCCCEEEECCCCHHHHCCCCEEEEEEECCCCCEEEECCCCEEEECCHHHCCCCCC
FKIMMNVGNPDRAFDFAQIPNEGIGLARLEFIINRMIGVHPKALLNIDSLPRETRAAVLA
EEEEEECCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHCCCCHHHCCCCCCCHHHHHHHHH
RTAGYTSPIDFYVEKLVEGISTLAAAFAEKPVIVRMSDFKSNEYANLIGGKLYEPEEENP
HHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHCCCCCCCCCCCC
MLGFRGASRYVSDNFRDCFELECRALKKARDEMGLTNIQIMIPFVRTVAEAKRVIELLAL
CEEECCCCHHHHCCHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHH
NGLKRGENGLKVIMMCELPTNALLAEQFLEHFDGFSIGSNDLTQLTLGLDRDSGIVSHLF
CCCCCCCCCCEEEEEEECCCHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCHHHHHH
DERDPAVKALLSMAIHACRKAGKYVGICGQGPSDHPDLAKWLMEQGIESVSLNPDSVLDT
CCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHCCCCEECCCCHHHHHH
WFFLAEEKTKQA
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10710307 [H]