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The map label for this gene is lipA [H]

Identifier: 50085376

GI number: 50085376

Start: 2244827

End: 2245840

Strand: Direct

Name: lipA [H]

Synonym: ACIAD2275

Alternate gene names: 50085376

Gene position: 2244827-2245840 (Clockwise)

Preceding gene: 50085374

Following gene: 50085377

Centisome position: 62.38

GC content: 44.08

Gene sequence:

>1014_bases
ATGTCTGAAAGCCGTAAACCTGAACAGGGTGTAAAACTCCGTGGTGCCGAAAAAGTAGCCCGAATTCCTGTAAAAGTTGT
ACCTACGGTTGAAGTACCGCGTAAACCAGACTGGATTCGCGTCAAGATGACTGCGCCAGAAGAAGTGCAACGCATCAAAA
CCACATTACGATCACAAAAATTGCATACTGTATGTGAAGAAGCGGCTTGCCCCAATCTGCCAGAATGTTTTGGGGGCGGT
ACAGCGACATTTATGATTATGGGCGATATCTGTACACGTCGCTGCCCATTCTGCGATGTGGCTCATGGTCGTCCAAATGC
TTTAGATGCAGATGAACCTCGCCATATGGCAGAAACCATTGCAAATCTTAAGCTTAAGTATGCGGTAATTACCTCGGTAG
ACCGTGATGATTTGCTCGATGGTGGCGCTCAGCACTTTGTTGATTGTATTAAAGAAGCGCGCGCATTAAGCCCAAATACA
CTGCTTGAAATTCTGGTACCAGACTTCCGTGGTCGTATGGATATTGCACTTCGCATCATGACTGAATGCCCACCAGATGT
GTTTAATCACAATATTGAAACTGTTCCTCGTTTGTATAAAGCCATGCGTCCAGGTTCAGATTATCAGCATTCTTTAACAC
TGCTGAAAATGTTTAAAGAATATTGCCCAGATATTCCAACTAAATGTGGATTAATGGTCGGTATTGGTGAAACTGAAGAT
GAAGTCATTGCTTTACTTGATGACTTGCGTGCACATGATGTTGATTACGTCACGATTGGTCAATACTTACAGCCTTCAAA
ACAACATGCTCCTATTGATCGTTTTGTAACACCAGAAGAATTTGAACGTTATGCGGAACATGGTCGTCAACTTGGCTTTA
GAAATATCTGGTCGGCCCCAATGGTTCGCTCAAGCTACTTTGCAGATCGTCAATACCACGGCGAACCTGTACCAGCTGTA
CGCCGTAAAGTAGATCCTGCCAAAAAGATTGCTGTTCAGGCTATCGAAGCTTAA

Upstream 100 bases:

>100_bases
ACATTTTTTTTATGAATAATGAATTTGCCCTATTTATCATCAAGTCCGTTATAATAGTCAACGATAGAAATTCACGCGAA
TAACATTGGAATAGTTAACT

Downstream 100 bases:

>100_bases
TTAGTTTCTTCTTATTTAGAAAACCTCTCAAAACTTTGGGAGGTTTTTATTTTTTAGTAACCTTTTGTGACAAATCAATA
ACCTAACTCCCCTGCGAAAA

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA [H]

Number of amino acids: Translated: 337; Mature: 336

Protein sequence:

>337_residues
MSESRKPEQGVKLRGAEKVARIPVKVVPTVEVPRKPDWIRVKMTAPEEVQRIKTTLRSQKLHTVCEEAACPNLPECFGGG
TATFMIMGDICTRRCPFCDVAHGRPNALDADEPRHMAETIANLKLKYAVITSVDRDDLLDGGAQHFVDCIKEARALSPNT
LLEILVPDFRGRMDIALRIMTECPPDVFNHNIETVPRLYKAMRPGSDYQHSLTLLKMFKEYCPDIPTKCGLMVGIGETED
EVIALLDDLRAHDVDYVTIGQYLQPSKQHAPIDRFVTPEEFERYAEHGRQLGFRNIWSAPMVRSSYFADRQYHGEPVPAV
RRKVDPAKKIAVQAIEA

Sequences:

>Translated_337_residues
MSESRKPEQGVKLRGAEKVARIPVKVVPTVEVPRKPDWIRVKMTAPEEVQRIKTTLRSQKLHTVCEEAACPNLPECFGGG
TATFMIMGDICTRRCPFCDVAHGRPNALDADEPRHMAETIANLKLKYAVITSVDRDDLLDGGAQHFVDCIKEARALSPNT
LLEILVPDFRGRMDIALRIMTECPPDVFNHNIETVPRLYKAMRPGSDYQHSLTLLKMFKEYCPDIPTKCGLMVGIGETED
EVIALLDDLRAHDVDYVTIGQYLQPSKQHAPIDRFVTPEEFERYAEHGRQLGFRNIWSAPMVRSSYFADRQYHGEPVPAV
RRKVDPAKKIAVQAIEA
>Mature_336_residues
SESRKPEQGVKLRGAEKVARIPVKVVPTVEVPRKPDWIRVKMTAPEEVQRIKTTLRSQKLHTVCEEAACPNLPECFGGGT
ATFMIMGDICTRRCPFCDVAHGRPNALDADEPRHMAETIANLKLKYAVITSVDRDDLLDGGAQHFVDCIKEARALSPNTL
LEILVPDFRGRMDIALRIMTECPPDVFNHNIETVPRLYKAMRPGSDYQHSLTLLKMFKEYCPDIPTKCGLMVGIGETEDE
VIALLDDLRAHDVDYVTIGQYLQPSKQHAPIDRFVTPEEFERYAEHGRQLGFRNIWSAPMVRSSYFADRQYHGEPVPAVR
RKVDPAKKIAVQAIEA

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives [H]

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family [H]

Homologues:

Organism=Homo sapiens, GI37577166, Length=282, Percent_Identity=44.3262411347518, Blast_Score=251, Evalue=9e-67,
Organism=Homo sapiens, GI37577164, Length=247, Percent_Identity=44.1295546558704, Blast_Score=216, Evalue=2e-56,
Organism=Escherichia coli, GI1786846, Length=320, Percent_Identity=58.125, Blast_Score=380, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI32564533, Length=289, Percent_Identity=46.0207612456747, Blast_Score=241, Evalue=5e-64,
Organism=Saccharomyces cerevisiae, GI6324770, Length=291, Percent_Identity=40.893470790378, Blast_Score=235, Evalue=1e-62,
Organism=Drosophila melanogaster, GI221513272, Length=282, Percent_Identity=44.6808510638298, Blast_Score=249, Evalue=2e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.8.1.8 [H]

Molecular weight: Translated: 38035; Mature: 37904

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSESRKPEQGVKLRGAEKVARIPVKVVPTVEVPRKPDWIRVKMTAPEEVQRIKTTLRSQK
CCCCCCCCCCCEECCCHHHHHCCEEEECCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHH
LHTVCEEAACPNLPECFGGGTATFMIMGDICTRRCPFCDVAHGRPNALDADEPRHMAETI
HHHHHHHHCCCCCHHHHCCCCEEEEEEEHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHH
ANLKLKYAVITSVDRDDLLDGGAQHFVDCIKEARALSPNTLLEILVPDFRGRMDIALRIM
HHCEEEEEEEECCCHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCHHHHHHHH
TECPPDVFNHNIETVPRLYKAMRPGSDYQHSLTLLKMFKEYCPDIPTKCGLMVGIGETED
HHCCHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCHH
EVIALLDDLRAHDVDYVTIGQYLQPSKQHAPIDRFVTPEEFERYAEHGRQLGFRNIWSAP
HHHHHHHHHHHCCCCEEEHHHHHCCCCCCCCHHHCCCHHHHHHHHHHHHHCCHHHHCCCC
MVRSSYFADRQYHGEPVPAVRRKVDPAKKIAVQAIEA
HHHHHHHHCCCCCCCCCHHHHHCCCHHHHHHHHHHCC
>Mature Secondary Structure 
SESRKPEQGVKLRGAEKVARIPVKVVPTVEVPRKPDWIRVKMTAPEEVQRIKTTLRSQK
CCCCCCCCCCEECCCHHHHHCCEEEECCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHH
LHTVCEEAACPNLPECFGGGTATFMIMGDICTRRCPFCDVAHGRPNALDADEPRHMAETI
HHHHHHHHCCCCCHHHHCCCCEEEEEEEHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHH
ANLKLKYAVITSVDRDDLLDGGAQHFVDCIKEARALSPNTLLEILVPDFRGRMDIALRIM
HHCEEEEEEEECCCHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCHHHHHHHH
TECPPDVFNHNIETVPRLYKAMRPGSDYQHSLTLLKMFKEYCPDIPTKCGLMVGIGETED
HHCCHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCHH
EVIALLDDLRAHDVDYVTIGQYLQPSKQHAPIDRFVTPEEFERYAEHGRQLGFRNIWSAP
HHHHHHHHHHHCCCCEEEHHHHHCCCCCCCCHHHCCCHHHHHHHHHHHHHCCHHHHCCCC
MVRSSYFADRQYHGEPVPAVRRKVDPAKKIAVQAIEA
HHHHHHHHCCCCCCCCCHHHHHCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA