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The map label for this gene is ahpF [H]

Identifier: 50085355

GI number: 50085355

Start: 2221393

End: 2222997

Strand: Reverse

Name: ahpF [H]

Synonym: ACIAD2250

Alternate gene names: 50085355

Gene position: 2222997-2221393 (Counterclockwise)

Preceding gene: 50085356

Following gene: 50085354

Centisome position: 61.77

GC content: 45.05

Gene sequence:

>1605_bases
ATGATCCCCATCAAGTTAAAGAATTTACTCCTTTGGAGCTTCAACATGTTAGACCAAAATATCAAAACTCAACTTAAAGC
TTATTTAGAACGTTTGGAAAGCCCAATTGAATTGGTCGCTGCACTCGATGACTCAGACAAAGCTGCCAAAATCAAAGAAC
TGGTCACTGAAATTGCAGAACTCTCCAACCAGGTGACAGCACGTTTTGATGGTCAGAATACACGCCGTCCAAGTTTCGGT
ATTGCCAAAGCCGGTGAACAACCTCGTGTGTTCTTTGCAGGCTTGCCGATGGGGCATGAGTTTACCTCACTCATTCTGGC
ATTGTTACAAACCTCAGGTTATGCACCTAAAGTGTCTGATGAAATCTTAAACCAGATTAAAGGCCTTAACTTAAAAGCTG
ATTTTGATGTGTTTGTTTCTTTAAGCTGTCATAACTGTCCAGACGTGGTCCAGGCGTTAAACTTGATTGCGATCTATAAT
CCAAACAGTACTGCGACCATGATTGATGGTGCTTTCTTTCAGGATGAAGTCGAAGAACGCAAAATCATGGCCGTACCAAT
GGTGTTCCAGAATGGCAATCACATCGGTCAGGGTCGTATGACGCTAGAGGAAATTGTCGCGAAACTGGACAGCAATGCTG
CTGAGAAAGATGCCGCTGCCATTAATGCAAAAGATGCTTTTGATGTATTGGTCGTAGGTGCTGGTCCTGCTGGTGGTACT
GCCGCGATCTATGCAGCACGTAAAGGTATTCGTACTGGTATGATTGCCGAGCGCTTTGGTGGTCAGGTGATGGACACCAT
GGACATTGAAAATTTCACCTCTGTACAAAAAACGGTGGGGCCAAAGTTTGCTGCGGATATGGAAGCGCATGTCCGTGAAT
ATGATGTTGATATCATGAATTTGCAACGTGTCAGTAAGATCGTGGGTGCAGATCAAACTGCTTCAGGTCTGGTTGAAGTT
GAACTGGAAAATGGGGCTAAGGTTGAATCAAAAACCGTGATTTTATCGACGGGTGCGCGCTGGAGAAAAATGGGGGTTCC
TGGTGAGCAGGAATATGCCACTCGTGGTGTCGCATATTGCCCACACTGTGATGGTCCATTGTTTAAAGGCAAGAAAGTTG
CGGTGATTGGTGGAGGTAACTCAGGTGTCGAAGCTGCAATTGATTTAGCAGGAATTGTTGAGCATGTAACGCTGGTAGAA
TTTGATACCAAACTTCGTGCAGATCAGGTATTGCAGGACAAGCTGAATAGTTTGCCAAATGCGACCATTATTACCAACGC
ACTTTCTACAGAGGTGATCGGTGATGGTGCTCAGGTGACGGGTTTGAAGTATAAAGATCGTGCGACAGATACAGAACACA
CGATTGAGCTGGCAGGGATTTTTGTACAGATTGGCTTGTTACCAAACACTGACTTTCTTAAAGGTTCGGTAGAGTTAAGC
CCACGAGGTGAGATCATCATCAATGAGCGCAATGAAACCAATGTGAAGGGTGTGTTTGCAGCGGGTGACTGTACCACAGT
GCCGTACAAGCAGATCATTATTGCCACAGGTGAAGGTGCCAAAGCGTCTCTGTCTGCTTTTGATTATATTATTCGTCATG
GCTGA

Upstream 100 bases:

>100_bases
CACACTTCGAATTGAACAAACATCTTAATCAATTTTAAAATTCAAAAAACCGATTAAAACAATAAAAACATACTGTTTCA
GCTATTCTAACTTTTATCGT

Downstream 100 bases:

>100_bases
TTGATTAGATAAAGATTCAAGTTACATTGGATTGACTTGATTAAATCACTTTACTTTACTTTACGGAGTCTGATCTTCTA
ACAGTCAGGCTCCTTTTTTT

Product: alkyl hydroperoxide reductase subunit F

Products: an alcohol; H2O; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 534; Mature: 534

Protein sequence:

>534_residues
MIPIKLKNLLLWSFNMLDQNIKTQLKAYLERLESPIELVAALDDSDKAAKIKELVTEIAELSNQVTARFDGQNTRRPSFG
IAKAGEQPRVFFAGLPMGHEFTSLILALLQTSGYAPKVSDEILNQIKGLNLKADFDVFVSLSCHNCPDVVQALNLIAIYN
PNSTATMIDGAFFQDEVEERKIMAVPMVFQNGNHIGQGRMTLEEIVAKLDSNAAEKDAAAINAKDAFDVLVVGAGPAGGT
AAIYAARKGIRTGMIAERFGGQVMDTMDIENFTSVQKTVGPKFAADMEAHVREYDVDIMNLQRVSKIVGADQTASGLVEV
ELENGAKVESKTVILSTGARWRKMGVPGEQEYATRGVAYCPHCDGPLFKGKKVAVIGGGNSGVEAAIDLAGIVEHVTLVE
FDTKLRADQVLQDKLNSLPNATIITNALSTEVIGDGAQVTGLKYKDRATDTEHTIELAGIFVQIGLLPNTDFLKGSVELS
PRGEIIINERNETNVKGVFAAGDCTTVPYKQIIIATGEGAKASLSAFDYIIRHG

Sequences:

>Translated_534_residues
MIPIKLKNLLLWSFNMLDQNIKTQLKAYLERLESPIELVAALDDSDKAAKIKELVTEIAELSNQVTARFDGQNTRRPSFG
IAKAGEQPRVFFAGLPMGHEFTSLILALLQTSGYAPKVSDEILNQIKGLNLKADFDVFVSLSCHNCPDVVQALNLIAIYN
PNSTATMIDGAFFQDEVEERKIMAVPMVFQNGNHIGQGRMTLEEIVAKLDSNAAEKDAAAINAKDAFDVLVVGAGPAGGT
AAIYAARKGIRTGMIAERFGGQVMDTMDIENFTSVQKTVGPKFAADMEAHVREYDVDIMNLQRVSKIVGADQTASGLVEV
ELENGAKVESKTVILSTGARWRKMGVPGEQEYATRGVAYCPHCDGPLFKGKKVAVIGGGNSGVEAAIDLAGIVEHVTLVE
FDTKLRADQVLQDKLNSLPNATIITNALSTEVIGDGAQVTGLKYKDRATDTEHTIELAGIFVQIGLLPNTDFLKGSVELS
PRGEIIINERNETNVKGVFAAGDCTTVPYKQIIIATGEGAKASLSAFDYIIRHG
>Mature_534_residues
MIPIKLKNLLLWSFNMLDQNIKTQLKAYLERLESPIELVAALDDSDKAAKIKELVTEIAELSNQVTARFDGQNTRRPSFG
IAKAGEQPRVFFAGLPMGHEFTSLILALLQTSGYAPKVSDEILNQIKGLNLKADFDVFVSLSCHNCPDVVQALNLIAIYN
PNSTATMIDGAFFQDEVEERKIMAVPMVFQNGNHIGQGRMTLEEIVAKLDSNAAEKDAAAINAKDAFDVLVVGAGPAGGT
AAIYAARKGIRTGMIAERFGGQVMDTMDIENFTSVQKTVGPKFAADMEAHVREYDVDIMNLQRVSKIVGADQTASGLVEV
ELENGAKVESKTVILSTGARWRKMGVPGEQEYATRGVAYCPHCDGPLFKGKKVAVIGGGNSGVEAAIDLAGIVEHVTLVE
FDTKLRADQVLQDKLNSLPNATIITNALSTEVIGDGAQVTGLKYKDRATDTEHTIELAGIFVQIGLLPNTDFLKGSVELS
PRGEIIINERNETNVKGVFAAGDCTTVPYKQIIIATGEGAKASLSAFDYIIRHG

Specific function: Serves to protect the cell against DNA damage by alkyl hydroperoxides. It can use either NADH or NADPH as electron donor for direct reduction of redox dyes or of alkyl hydroperoxides when combined with the AhpC protein [H]

COG id: COG3634

COG function: function code O; Alkyl hydroperoxide reductase, large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI87081763, Length=518, Percent_Identity=62.1621621621622, Blast_Score=647, Evalue=0.0,
Organism=Escherichia coli, GI1787114, Length=316, Percent_Identity=35.126582278481, Blast_Score=164, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6321898, Length=339, Percent_Identity=31.5634218289086, Blast_Score=162, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6320560, Length=312, Percent_Identity=32.6923076923077, Blast_Score=148, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012081
- InterPro:   IPR013027
- InterPro:   IPR002109
- InterPro:   IPR008255
- InterPro:   IPR001327
- InterPro:   IPR000103
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.6.4.- [C]

Molecular weight: Translated: 57589; Mature: 57589

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS00573 PYRIDINE_REDOX_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPIKLKNLLLWSFNMLDQNIKTQLKAYLERLESPIELVAALDDSDKAAKIKELVTEIAE
CCCEEECHHHEEEHHHHHHHHHHHHHHHHHHHCCHHHHHEECCCCCHHHHHHHHHHHHHH
LSNQVTARFDGQNTRRPSFGIAKAGEQPRVFFAGLPMGHEFTSLILALLQTSGYAPKVSD
HCCCEEEEECCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCHHH
EILNQIKGLNLKADFDVFVSLSCHNCPDVVQALNLIAIYNPNSTATMIDGAFFQDEVEER
HHHHHHCCCCEEECCEEEEEEECCCCHHHHHHCCEEEEECCCCCEEEECCHHHHHHHHCC
KIMAVPMVFQNGNHIGQGRMTLEEIVAKLDSNAAEKDAAAINAKDAFDVLVVGAGPAGGT
EEEEEEEEEECCCEECCCCHHHHHHHHHHCCCCCCCHHHEECCCCCEEEEEEECCCCCCH
AAIYAARKGIRTGMIAERFGGQVMDTMDIENFTSVQKTVGPKFAADMEAHVREYDVDIMN
HHHHHHHHCCHHCHHHHHHCCCEEECCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHH
LQRVSKIVGADQTASGLVEVELENGAKVESKTVILSTGARWRKMGVPGEQEYATRGVAYC
HHHHHHHHCCCCCCCCEEEEEECCCCEEECCEEEEECCCCEEECCCCCCHHHHHCCCEEC
PHCDGPLFKGKKVAVIGGGNSGVEAAIDLAGIVEHVTLVEFDTKLRADQVLQDKLNSLPN
CCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHEEEEECCHHHHHHHHHHHHHCCCC
ATIITNALSTEVIGDGAQVTGLKYKDRATDTEHTIELAGIFVQIGLLPNTDFLKGSVELS
CEEEEHHHHHHHCCCCCEEECEEECCCCCCCHHHHEEEEEEEEEECCCCCCCCCCEEEEC
PRGEIIINERNETNVKGVFAAGDCTTVPYKQIIIATGEGAKASLSAFDYIIRHG
CCCCEEEECCCCCCCEEEEECCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MIPIKLKNLLLWSFNMLDQNIKTQLKAYLERLESPIELVAALDDSDKAAKIKELVTEIAE
CCCEEECHHHEEEHHHHHHHHHHHHHHHHHHHCCHHHHHEECCCCCHHHHHHHHHHHHHH
LSNQVTARFDGQNTRRPSFGIAKAGEQPRVFFAGLPMGHEFTSLILALLQTSGYAPKVSD
HCCCEEEEECCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCHHH
EILNQIKGLNLKADFDVFVSLSCHNCPDVVQALNLIAIYNPNSTATMIDGAFFQDEVEER
HHHHHHCCCCEEECCEEEEEEECCCCHHHHHHCCEEEEECCCCCEEEECCHHHHHHHHCC
KIMAVPMVFQNGNHIGQGRMTLEEIVAKLDSNAAEKDAAAINAKDAFDVLVVGAGPAGGT
EEEEEEEEEECCCEECCCCHHHHHHHHHHCCCCCCCHHHEECCCCCEEEEEEECCCCCCH
AAIYAARKGIRTGMIAERFGGQVMDTMDIENFTSVQKTVGPKFAADMEAHVREYDVDIMN
HHHHHHHHCCHHCHHHHHHCCCEEECCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHH
LQRVSKIVGADQTASGLVEVELENGAKVESKTVILSTGARWRKMGVPGEQEYATRGVAYC
HHHHHHHHCCCCCCCCEEEEEECCCCEEECCEEEEECCCCEEECCCCCCHHHHHCCCEEC
PHCDGPLFKGKKVAVIGGGNSGVEAAIDLAGIVEHVTLVEFDTKLRADQVLQDKLNSLPN
CCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHEEEEECCHHHHHHHHHHHHHCCCC
ATIITNALSTEVIGDGAQVTGLKYKDRATDTEHTIELAGIFVQIGLLPNTDFLKGSVELS
CEEEEHHHHHHHCCCCCEEECEEECCCCCCCHHHHEEEEEEEEEECCCCCCCCCCEEEEC
PRGEIIINERNETNVKGVFAAGDCTTVPYKQIIIATGEGAKASLSAFDYIIRHG
CCCCEEEECCCCCCCEEEEECCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ROOH; Proton; NADH [C]

Specific reaction: ROOH + Proton + NADH = an alcohol + H2O + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]