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The map label for this gene is trmJ [H]

Identifier: 50085211

GI number: 50085211

Start: 2075934

End: 2076749

Strand: Direct

Name: trmJ [H]

Synonym: ACIAD2090

Alternate gene names: 50085211

Gene position: 2075934-2076749 (Clockwise)

Preceding gene: 50085210

Following gene: 50085212

Centisome position: 57.69

GC content: 41.91

Gene sequence:

>816_bases
TTGACGCATGTACGAATTGTAATGGTAAATACGACACTGCCTGCAAATATCGGTAGCGCATTACGTGCGATGAAAACGAT
GGGACTATCTCAACTGGTTCTGGTTGCACCTAAAACTTACCCTCATCCTGATATTGATGCGCTGGCCGCAGGTGCGCAAG
ACCTGGTTGAAAAGATTAAAATTGTAGATACGCTAGAACAAGCCATTCAGGATTGTCATATTGTATTTGGTACAAGTGCT
CGAAGCCGTACTATTCCCTGGCCACTACTCGATGCGCGGCCTGCAGCAGAACTTTCAATGGATGCTGTTATCACTAGACA
GCAAGAAATTGCAATTGTGTTTGGTCGTGAAGATCGTGGACTGACCAACGAAGAACTTGCCTTAGCTAATTATCACCTGA
CCATCCCTGTCAATACAGATTATGGTGTACTCAATGTCGCCCAAGCGATTCAAGTCATTTGTTATGAAATGAGAATGGCA
ACTCTTGCCCAAGTACAAAATGATCAGGCATCTTCTGATTCTCAAAAAATGCAGGTAACTGAAAAAATCGAGATGGATTG
GGATGAACCTCTGGTTAATCATCAGCAAATGGAAGAGTTCTATCCACATCTTGAAAAAATGTTAGTCGATATAGAGTTTC
TTGATCCTCAAAATCCAAGATTATTACCATTAAGACTTCGTCGACTATTTGGTCGTGCACAATTAGATCGAATGGAATAC
CATTTGCTGCGAGGCATTTTCAGTCGAGTTCAAGCACTCAGTCATGGAAAATGGAAAAAAACAGCTCTGGACAAGGAGGA
ACAGTCAGATGTTTAA

Upstream 100 bases:

>100_bases
GAAGCTATGGATTTATATCAGGCCGAAGTCTCACAATACTCTTAAGTTTTGATTTGGATAATTTATGAATACTGCTGACA
ATGCGTCAATCAATGAATAT

Downstream 100 bases:

>100_bases
ACAGTTAAAAGAAGATATTCAGGCCGTTTTTGCTCGTGATCCTGCTGCTCGCAATACGCTGGAGGTGTTGACAACATATC
CAGGTATTCATGCGCTGATT

Product: tRNA/rRNA methyltransferase

Products: NA

Alternate protein names: tRNA Cm32/Um32 methyltransferase [H]

Number of amino acids: Translated: 271; Mature: 270

Protein sequence:

>271_residues
MTHVRIVMVNTTLPANIGSALRAMKTMGLSQLVLVAPKTYPHPDIDALAAGAQDLVEKIKIVDTLEQAIQDCHIVFGTSA
RSRTIPWPLLDARPAAELSMDAVITRQQEIAIVFGREDRGLTNEELALANYHLTIPVNTDYGVLNVAQAIQVICYEMRMA
TLAQVQNDQASSDSQKMQVTEKIEMDWDEPLVNHQQMEEFYPHLEKMLVDIEFLDPQNPRLLPLRLRRLFGRAQLDRMEY
HLLRGIFSRVQALSHGKWKKTALDKEEQSDV

Sequences:

>Translated_271_residues
MTHVRIVMVNTTLPANIGSALRAMKTMGLSQLVLVAPKTYPHPDIDALAAGAQDLVEKIKIVDTLEQAIQDCHIVFGTSA
RSRTIPWPLLDARPAAELSMDAVITRQQEIAIVFGREDRGLTNEELALANYHLTIPVNTDYGVLNVAQAIQVICYEMRMA
TLAQVQNDQASSDSQKMQVTEKIEMDWDEPLVNHQQMEEFYPHLEKMLVDIEFLDPQNPRLLPLRLRRLFGRAQLDRMEY
HLLRGIFSRVQALSHGKWKKTALDKEEQSDV
>Mature_270_residues
THVRIVMVNTTLPANIGSALRAMKTMGLSQLVLVAPKTYPHPDIDALAAGAQDLVEKIKIVDTLEQAIQDCHIVFGTSAR
SRTIPWPLLDARPAAELSMDAVITRQQEIAIVFGREDRGLTNEELALANYHLTIPVNTDYGVLNVAQAIQVICYEMRMAT
LAQVQNDQASSDSQKMQVTEKIEMDWDEPLVNHQQMEEFYPHLEKMLVDIEFLDPQNPRLLPLRLRRLFGRAQLDRMEYH
LLRGIFSRVQALSHGKWKKTALDKEEQSDV

Specific function: Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA [H]

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788881, Length=259, Percent_Identity=43.2432432432432, Blast_Score=196, Evalue=2e-51,
Organism=Escherichia coli, GI1790865, Length=185, Percent_Identity=36.7567567567568, Blast_Score=107, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 30738; Mature: 30607

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHVRIVMVNTTLPANIGSALRAMKTMGLSQLVLVAPKTYPHPDIDALAAGAQDLVEKIK
CCEEEEEEEECCCCCHHHHHHHHHHHCCHHHEEEECCCCCCCCCHHHHHCCHHHHHHHHH
IVDTLEQAIQDCHIVFGTSARSRTIPWPLLDARPAAELSMDAVITRQQEIAIVFGREDRG
HHHHHHHHHHHCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEEECCCCC
LTNEELALANYHLTIPVNTDYGVLNVAQAIQVICYEMRMATLAQVQNDQASSDSQKMQVT
CCCCCEEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
EKIEMDWDEPLVNHQQMEEFYPHLEKMLVDIEFLDPQNPRLLPLRLRRLFGRAQLDRMEY
HHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHCHHHHHHHHH
HLLRGIFSRVQALSHGKWKKTALDKEEQSDV
HHHHHHHHHHHHHHCCCHHHHHCCCHHHCCC
>Mature Secondary Structure 
THVRIVMVNTTLPANIGSALRAMKTMGLSQLVLVAPKTYPHPDIDALAAGAQDLVEKIK
CEEEEEEEECCCCCHHHHHHHHHHHCCHHHEEEECCCCCCCCCHHHHHCCHHHHHHHHH
IVDTLEQAIQDCHIVFGTSARSRTIPWPLLDARPAAELSMDAVITRQQEIAIVFGREDRG
HHHHHHHHHHHCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEEECCCCC
LTNEELALANYHLTIPVNTDYGVLNVAQAIQVICYEMRMATLAQVQNDQASSDSQKMQVT
CCCCCEEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
EKIEMDWDEPLVNHQQMEEFYPHLEKMLVDIEFLDPQNPRLLPLRLRRLFGRAQLDRMEY
HHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHCHHHHHHHHH
HLLRGIFSRVQALSHGKWKKTALDKEEQSDV
HHHHHHHHHHHHHHCCCHHHHHCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14528314 [H]