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The map label for this gene is purT

Identifier: 50085199

GI number: 50085199

Start: 2058547

End: 2059761

Strand: Direct

Name: purT

Synonym: ACIAD2078

Alternate gene names: 50085199

Gene position: 2058547-2059761 (Clockwise)

Preceding gene: 50085198

Following gene: 50085200

Centisome position: 57.2

GC content: 42.8

Gene sequence:

>1215_bases
ATGAGCGTCACGATTGGTACTCCCCTTCAATCTTCTGCATTTAAAGTTTTATTATTAGGTTCGGGAGAGCTTGGAAAAGA
GGTCGTCATTTCATTACAGCGCCTTGGGGTCGAAGTTCATGCGGCAGATCGCTACGATCATGCGCCAGCAATGCAAGTTG
CACATTATTCATATACGCTTAATATGGCAGATCCTGTTGAGCTGAAAAAACTGATTGAAAGCATTCAACCTGATTTAATT
GTTCCAGAAATTGAAGCAATTGCCACAGAAGTTTTAATTGAAGTTGAAAATAATAAACTTGCCACAGTCATTCCTTCTGC
CAAAGCTGTAAATCTCACGATGAATCGTGAAGGTATTCGTCGCTTAGCTGCAGAAGAACTTGGTTTACCAACATCGGCAT
ATCGTTTTGCCGACTCTTTAGAAAGCTTCCGAGCTGCGTGTGATGACATTGGTTACCCAAACTTTGTAAAACCTGTAATG
TCTTCTTCTGGTAAAGGTCAGTCACGTGTCAAAAGTTTTGAAGAAGTTGATGCTGCTTGGGATTATGCACAAACTGGCGG
GCGTGTGAAGCAAGGCAAGGTAATTGTTGAATCACAAATTGATTTTGATTTTGAAATTACTTTACTTACTGTACGTGCTA
AAAACCCAGAAACAGGTGCAATTGAAACTCAGTATTGTGATCCGATTGGCCACCGTCAAGACGCTGGTGATTATGTTGAA
AGCTGGCAACCGCAAGCGATGACAGATGCAGCACTTACAGAAGCAAAGCGTATCGCACATAAGGTTACTGTGGCACTGGG
TGGTTGTGGTATTTTTGGTGTCGAACTCTTCATCAAAGGTGATCACGTGTGGTTTAGTGAGGTTTCTCCACGCCCACATG
ACACAGGTTTAGTGACTTTAGCATCTCAATTTCAAAGTGAATTTGAACTTCATGCACGCGCAATTCTAGGCTTACCAGTC
AATACAACTCGTCATAGCGTGGCAGCAAGTGCTGTTATTTATGCGGGTGTAGATGCGGACAACCTCAGTTTCAGTCAGCT
CAATCAAGCATTAGCGCATCCAGAAACTGATCTACGTTTATTTGGAAAACCAGAAGGCTTTAAAAGACGTCGTATGGGCG
TAGCCACTGCACGAGCTGAAACTACCGATCAAGCTAGAGAATTAGCAACCCAAGCCGCTGAACACGTCCAAGTTATTGTT
GCAAGTAATCAATAA

Upstream 100 bases:

>100_bases
TATTAAACAACAAAAGTCATTTTTTTATGTGAATGTAAGCTTAATTCATGCAAGAGTTCTGTTATGCTTAAGAACCTTAC
TTCTTGCATGGATGATTCGC

Downstream 100 bases:

>100_bases
ACTGAGTATGAGACCATTTTTATGATTATAACTTCACCATTGCTCGACTACGTCACCAGTCATCAAGATATTAAAGCAAT
TAATCAATGGCGCGCCGATG

Product: phosphoribosylglycinamide formyltransferase 2

Products: 5'-Phosphoribosyl-N-Formylglycinamide; Pyrophosphate; acetylphosphate; ADP [C]

Alternate protein names: GART 2; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2

Number of amino acids: Translated: 404; Mature: 403

Protein sequence:

>404_residues
MSVTIGTPLQSSAFKVLLLGSGELGKEVVISLQRLGVEVHAADRYDHAPAMQVAHYSYTLNMADPVELKKLIESIQPDLI
VPEIEAIATEVLIEVENNKLATVIPSAKAVNLTMNREGIRRLAAEELGLPTSAYRFADSLESFRAACDDIGYPNFVKPVM
SSSGKGQSRVKSFEEVDAAWDYAQTGGRVKQGKVIVESQIDFDFEITLLTVRAKNPETGAIETQYCDPIGHRQDAGDYVE
SWQPQAMTDAALTEAKRIAHKVTVALGGCGIFGVELFIKGDHVWFSEVSPRPHDTGLVTLASQFQSEFELHARAILGLPV
NTTRHSVAASAVIYAGVDADNLSFSQLNQALAHPETDLRLFGKPEGFKRRRMGVATARAETTDQARELATQAAEHVQVIV
ASNQ

Sequences:

>Translated_404_residues
MSVTIGTPLQSSAFKVLLLGSGELGKEVVISLQRLGVEVHAADRYDHAPAMQVAHYSYTLNMADPVELKKLIESIQPDLI
VPEIEAIATEVLIEVENNKLATVIPSAKAVNLTMNREGIRRLAAEELGLPTSAYRFADSLESFRAACDDIGYPNFVKPVM
SSSGKGQSRVKSFEEVDAAWDYAQTGGRVKQGKVIVESQIDFDFEITLLTVRAKNPETGAIETQYCDPIGHRQDAGDYVE
SWQPQAMTDAALTEAKRIAHKVTVALGGCGIFGVELFIKGDHVWFSEVSPRPHDTGLVTLASQFQSEFELHARAILGLPV
NTTRHSVAASAVIYAGVDADNLSFSQLNQALAHPETDLRLFGKPEGFKRRRMGVATARAETTDQARELATQAAEHVQVIV
ASNQ
>Mature_403_residues
SVTIGTPLQSSAFKVLLLGSGELGKEVVISLQRLGVEVHAADRYDHAPAMQVAHYSYTLNMADPVELKKLIESIQPDLIV
PEIEAIATEVLIEVENNKLATVIPSAKAVNLTMNREGIRRLAAEELGLPTSAYRFADSLESFRAACDDIGYPNFVKPVMS
SSGKGQSRVKSFEEVDAAWDYAQTGGRVKQGKVIVESQIDFDFEITLLTVRAKNPETGAIETQYCDPIGHRQDAGDYVES
WQPQAMTDAALTEAKRIAHKVTVALGGCGIFGVELFIKGDHVWFSEVSPRPHDTGLVTLASQFQSEFELHARAILGLPVN
TTRHSVAASAVIYAGVDADNLSFSQLNQALAHPETDLRLFGKPEGFKRRRMGVATARAETTDQARELATQAAEHVQVIVA
SNQ

Specific function: Catalyzes two reactions:the first one is the production of beta-formyl glycinamide ribonucleotide (GAR) from formate, ATP and beta GAR; the second, a side reaction, is the production of acetyl phosphate and ADP from acetate and ATP

COG id: COG0027

COG function: function code F; Formate-dependent phosphoribosylglycinamide formyltransferase (GAR transformylase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1788155, Length=395, Percent_Identity=58.2278481012658, Blast_Score=431, Evalue=1e-122,
Organism=Saccharomyces cerevisiae, GI6324702, Length=401, Percent_Identity=25.6857855361596, Blast_Score=110, Evalue=5e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PURT_ACIAD (Q6FAM6)

Other databases:

- EMBL:   CR543861
- RefSeq:   YP_046709.1
- HSSP:   P33221
- ProteinModelPortal:   Q6FAM6
- SMR:   Q6FAM6
- STRING:   Q6FAM6
- GeneID:   2880910
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD2078
- NMPDR:   fig|62977.3.peg.1971
- eggNOG:   COG0027
- HOGENOM:   HBG516369
- OMA:   PDYIVPE
- PhylomeDB:   Q6FAM6
- ProtClustDB:   PRK09288
- BioCyc:   ASP62977:ACIAD2078-MONOMER
- HAMAP:   MF_01643
- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR005862
- InterPro:   IPR011054
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- PANTHER:   PTHR23047:SF2
- TIGRFAMs:   TIGR01142

Pfam domain/function: PF02222 ATP-grasp; SSF52440 PreATP-grasp-like; SSF51246 Rudmnt_hyb_motif

EC number: 2.1.2.- [C]

Molecular weight: Translated: 43972; Mature: 43841

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: PS50975 ATP_GRASP; PS00761 SPASE_I_3

Important sites: BINDING 83-83 BINDING 116-116 BINDING 157-157 BINDING 205-205 BINDING 275-275 BINDING 287-287 BINDING 294-294 BINDING 363-363

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVTIGTPLQSSAFKVLLLGSGELGKEVVISLQRLGVEVHAADRYDHAPAMQVAHYSYTL
CEEEECCCCCCCCEEEEEECCCCCCHHHHHHHHHCCEEEEECCCCCCCCHHEEEEEEEEE
NMADPVELKKLIESIQPDLIVPEIEAIATEVLIEVENNKLATVIPSAKAVNLTMNREGIR
CCCCHHHHHHHHHHCCCCEECCHHHHHHEEEEEEECCCEEEEEECCCEEEEEEECHHHHH
RLAAEELGLPTSAYRFADSLESFRAACDDIGYPNFVKPVMSSSGKGQSRVKSFEEVDAAW
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHH
DYAQTGGRVKQGKVIVESQIDFDFEITLLTVRAKNPETGAIETQYCDPIGHRQDAGDYVE
HHHHCCCEEECCEEEEEECCCCEEEEEEEEEEECCCCCCCEECCCCCCCCCCCCCCHHHH
SWQPQAMTDAALTEAKRIAHKVTVALGGCGIFGVELFIKGDHVWFSEVSPRPHDTGLVTL
CCCCCHHHHHHHHHHHHHHHHEEEEECCCCEEEEEEEEECCEEEEECCCCCCCCCHHHHH
ASQFQSEFELHARAILGLPVNTTRHSVAASAVIYAGVDADNLSFSQLNQALAHPETDLRL
HHHHHHHHHHHHHEEEECCCCCCHHHHHHEEEEEECCCCCCCCHHHHHHHHHCCCCCEEE
FGKPEGFKRRRMGVATARAETTDQARELATQAAEHVQVIVASNQ
ECCCCCHHHHHCCHHHHHCHHHHHHHHHHHHHHHHEEEEEECCC
>Mature Secondary Structure 
SVTIGTPLQSSAFKVLLLGSGELGKEVVISLQRLGVEVHAADRYDHAPAMQVAHYSYTL
EEEECCCCCCCCEEEEEECCCCCCHHHHHHHHHCCEEEEECCCCCCCCHHEEEEEEEEE
NMADPVELKKLIESIQPDLIVPEIEAIATEVLIEVENNKLATVIPSAKAVNLTMNREGIR
CCCCHHHHHHHHHHCCCCEECCHHHHHHEEEEEEECCCEEEEEECCCEEEEEEECHHHHH
RLAAEELGLPTSAYRFADSLESFRAACDDIGYPNFVKPVMSSSGKGQSRVKSFEEVDAAW
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHH
DYAQTGGRVKQGKVIVESQIDFDFEITLLTVRAKNPETGAIETQYCDPIGHRQDAGDYVE
HHHHCCCEEECCEEEEEECCCCEEEEEEEEEEECCCCCCCEECCCCCCCCCCCCCCHHHH
SWQPQAMTDAALTEAKRIAHKVTVALGGCGIFGVELFIKGDHVWFSEVSPRPHDTGLVTL
CCCCCHHHHHHHHHHHHHHHHEEEEECCCCEEEEEEEEECCEEEEECCCCCCCCCHHHHH
ASQFQSEFELHARAILGLPVNTTRHSVAASAVIYAGVDADNLSFSQLNQALAHPETDLRL
HHHHHHHHHHHHHEEEECCCCCCHHHHHHEEEEEECCCCCCCCHHHHHHHHHCCCCCEEE
FGKPEGFKRRRMGVATARAETTDQARELATQAAEHVQVIVASNQ
ECCCCCHHHHHCCHHHHHCHHHHHHHHHHHHHHHHEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Formate; 5'-Phospho-Ribosylglycinamide; acetate; ATP [C]

Specific reaction: Formate + ATP + 5'-Phospho-Ribosylglycinamide = 5'-Phosphoribosyl-N-Formylglycinamide + ADP + Pyrophosphate. acetate + ATP = acetylphosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA