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The map label for this gene is ruvC [H]

Identifier: 50085173

GI number: 50085173

Start: 2029228

End: 2029803

Strand: Direct

Name: ruvC [H]

Synonym: ACIAD2041

Alternate gene names: 50085173

Gene position: 2029228-2029803 (Clockwise)

Preceding gene: 50085171

Following gene: 50085175

Centisome position: 56.39

GC content: 42.88

Gene sequence:

>576_bases
ATGGGCAATATTTTATTTTTAAAGTCTTTTATGCCATTAATTATTGGAATTGATCCAGGTTCACGTTTAACAGGTTATGG
AATCATTGAAAAAAATGGTAATGAGCTGCGTTTTATCGATGCAGGCACAATTCGTACTGAAAGTCAGGACATGCCAGAAC
GATTAAAACGAATTTTTGCTGGTGTGGAACGAATTGTTAAATTTCATGGCCCAACACAAGCCGCGGTGGAACAAGTCTTT
ATGGCACAAAACCCCGACTCTGCACTTAAGCTAGGCCAAGCCAGAGGTGCTGCAATTGCAGCTTTGGTCAATCTGGATCT
GGAAGTAGCCGAATATACAGCAAGACAAATTAAACAATCGGTTGTGGGTTATGGTGCAGCCGACAAAGAACAGGTGCAGA
TGATGGTAATGCGTATATTAAATTTAACGATTAAACCACAATCCGATGCCGCAGATGCACTTGCAGCCGCCATTTGTCAT
GCACACGCTTCTGGCAGTATGAGTAAAATGGCTGTTTTAAATGCTTTAGGTGGTATGGCACGTGGACGAAGCCGATCCAG
TACCCGTCGTCGCTAA

Upstream 100 bases:

>100_bases
TTCATTTGAAACTTTGTCTATCTCAAAAATTAAAGGTTTTACGCAGTATCAAGACCGACTCAAATAGAATCAAGCTTTTT
TCATACGCTTATGTAAAAAT

Downstream 100 bases:

>100_bases
TCATTTTATGGGTGATTCACCACATGATAAATCGCTTTGGCCATATCATAGGCCTGAATCCCTCGTTGCCCATTCTGAGC
AAGTAAATCACCCGCTTGCG

Product: Holliday junction resolvase

Products: NA

Alternate protein names: Holliday junction nuclease ruvC; Holliday junction resolvase ruvC [H]

Number of amino acids: Translated: 191; Mature: 190

Protein sequence:

>191_residues
MGNILFLKSFMPLIIGIDPGSRLTGYGIIEKNGNELRFIDAGTIRTESQDMPERLKRIFAGVERIVKFHGPTQAAVEQVF
MAQNPDSALKLGQARGAAIAALVNLDLEVAEYTARQIKQSVVGYGAADKEQVQMMVMRILNLTIKPQSDAADALAAAICH
AHASGSMSKMAVLNALGGMARGRSRSSTRRR

Sequences:

>Translated_191_residues
MGNILFLKSFMPLIIGIDPGSRLTGYGIIEKNGNELRFIDAGTIRTESQDMPERLKRIFAGVERIVKFHGPTQAAVEQVF
MAQNPDSALKLGQARGAAIAALVNLDLEVAEYTARQIKQSVVGYGAADKEQVQMMVMRILNLTIKPQSDAADALAAAICH
AHASGSMSKMAVLNALGGMARGRSRSSTRRR
>Mature_190_residues
GNILFLKSFMPLIIGIDPGSRLTGYGIIEKNGNELRFIDAGTIRTESQDMPERLKRIFAGVERIVKFHGPTQAAVEQVFM
AQNPDSALKLGQARGAAIAALVNLDLEVAEYTARQIKQSVVGYGAADKEQVQMMVMRILNLTIKPQSDAADALAAAICHA
HASGSMSKMAVLNALGGMARGRSRSSTRRR

Specific function: Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leav

COG id: COG0817

COG function: function code L; Holliday junction resolvasome, endonuclease subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvC family [H]

Homologues:

Organism=Escherichia coli, GI1788170, Length=175, Percent_Identity=55.4285714285714, Blast_Score=169, Evalue=7e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012337
- InterPro:   IPR020563
- InterPro:   IPR002176 [H]

Pfam domain/function: PF02075 RuvC [H]

EC number: =3.1.22.4 [H]

Molecular weight: Translated: 20628; Mature: 20497

Theoretical pI: Translated: 10.46; Mature: 10.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNILFLKSFMPLIIGIDPGSRLTGYGIIEKNGNELRFIDAGTIRTESQDMPERLKRIFA
CCCEEEHHHCCCEEEECCCCCCEEEEEEEECCCCEEEEEECCCCCCCCCCHHHHHHHHHH
GVERIVKFHGPTQAAVEQVFMAQNPDSALKLGQARGAAIAALVNLDLEVAEYTARQIKQS
HHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHH
VVGYGAADKEQVQMMVMRILNLTIKPQSDAADALAAAICHAHASGSMSKMAVLNALGGMA
HHCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
RGRSRSSTRRR
CCCCCCCCCCC
>Mature Secondary Structure 
GNILFLKSFMPLIIGIDPGSRLTGYGIIEKNGNELRFIDAGTIRTESQDMPERLKRIFA
CCEEEHHHCCCEEEECCCCCCEEEEEEEECCCCEEEEEECCCCCCCCCCHHHHHHHHHH
GVERIVKFHGPTQAAVEQVFMAQNPDSALKLGQARGAAIAALVNLDLEVAEYTARQIKQS
HHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHH
VVGYGAADKEQVQMMVMRILNLTIKPQSDAADALAAAICHAHASGSMSKMAVLNALGGMA
HHCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
RGRSRSSTRRR
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA