Click here to switch to the map view.

The map label for this gene is metK

Identifier: 50085169

GI number: 50085169

Start: 2026565

End: 2027731

Strand: Direct

Name: metK

Synonym: ACIAD2037

Alternate gene names: 50085169

Gene position: 2026565-2027731 (Clockwise)

Preceding gene: 50085166

Following gene: 50085171

Centisome position: 56.32

GC content: 43.79

Gene sequence:

>1167_bases
ATGCGCGAGTACGCTGTTTTTACTTCGGAATCTGTAAGCGAAGGTCATCCCGATAAAATGGCAGATCAAATTAGTGACGC
TATTTTGGATGCAATCTTAAAAGAAGACCCATATGCACGGGTTGCTTGTGAAACACTCGTAAAAACAGGTGCTGTGGTTC
TAGCGGGTGAAATTACTACAACGGCCAATGTCGATTTTGAAGCAATTGTACGCCAAACCGTCAATGGTATTGGCTACCAT
CATTCAGATCTTGGATTTGATGGTTCAACCTGTGCCATTATCAACATGATTGGTAAGCAATCTCCAGAAATTGCTCAAGG
TGTAGATCGTCAGAAACCTGAAGATCAAGGCGCTGGTGACCAAGGCTTAATGTTTGGTTATGCAAGTCGTGAAACTGATG
TGCTTATGCCTGCACCAATTTCATACGCTCACCGCCTTATGGAACGTCAAGCTGAACTTCGTCGCTCAGGTGCTTTACCT
TGGTTACGTCCAGATGCTAAAAGCCAGGTTACCTTTGCTTATGAAAATGGTATGCCTGTACGTTTGGATGCAGTTGTACT
CTCCACTCAACACGATCCAGAAATTTCTCAGGCACAGTTAAAAGAAGCTGTGATTGAAGAAATTGTGAAACCTGTTATTC
CAAGCGAAATGTTTCATGCGGGTACTAAGTTTCATATCAACCCAACTGGTATGTTTGTGATTGGCGGCCCTGTAGGTGAC
TGCGGTCTCACTGGTCGTAAAATCATTGTAGATACTTATGGTGGTATGGCGCGTCACGGTGGTGGGGCTTTTTCAGGTAA
AGATCCTTCTAAAGTTGATCGCTCTGCTGCTTATGCTGGCCGTTATGTTGCGAAAAATATTGTTGCTGCTGGCTTGGCCG
ATAAATGTGAAATTCAGGTCAGTTATGCCATTGGTGTTGCTGAACCGACATCCATCTCGATTAATACCTTTAACACAGCA
AAAGTAGATGAAGAATTAATCATCGCCTTGGTTCGTGAGCATTTCGATTTACGTCCATATGGTATTACCCGTATGCTTGA
TTTGCTTCAACCGATGTATAAGCAGACCGCTGCTTACGGCCATTTTGGTCGTGAAGGTTCAGATACTGCCTTTACTTGGG
AAAAGACCGATAAGGTCGAAGTTCTTAAAGACGCTGCAGGTCTGTAA

Upstream 100 bases:

>100_bases
ATTGAAATAAATTCATGGATTTTAGTCAGCTTAAGCTAGTCCTAGCGTCTAAGTCGATGTAACATATTCCGTCTTTTGAA
TTAACTGTAATAGGACTTTC

Downstream 100 bases:

>100_bases
GATTAAATTCAAAAAAGTCCGATTTATCGGACTTTTTTATTTACATATAATTTAGTTAGCACAGCCTCTAGTTTTAATTA
TTCGATTTTGTGGTTTTATC

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase

Number of amino acids: Translated: 388; Mature: 388

Protein sequence:

>388_residues
MREYAVFTSESVSEGHPDKMADQISDAILDAILKEDPYARVACETLVKTGAVVLAGEITTTANVDFEAIVRQTVNGIGYH
HSDLGFDGSTCAIINMIGKQSPEIAQGVDRQKPEDQGAGDQGLMFGYASRETDVLMPAPISYAHRLMERQAELRRSGALP
WLRPDAKSQVTFAYENGMPVRLDAVVLSTQHDPEISQAQLKEAVIEEIVKPVIPSEMFHAGTKFHINPTGMFVIGGPVGD
CGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYAGRYVAKNIVAAGLADKCEIQVSYAIGVAEPTSISINTFNTA
KVDEELIIALVREHFDLRPYGITRMLDLLQPMYKQTAAYGHFGREGSDTAFTWEKTDKVEVLKDAAGL

Sequences:

>Translated_388_residues
MREYAVFTSESVSEGHPDKMADQISDAILDAILKEDPYARVACETLVKTGAVVLAGEITTTANVDFEAIVRQTVNGIGYH
HSDLGFDGSTCAIINMIGKQSPEIAQGVDRQKPEDQGAGDQGLMFGYASRETDVLMPAPISYAHRLMERQAELRRSGALP
WLRPDAKSQVTFAYENGMPVRLDAVVLSTQHDPEISQAQLKEAVIEEIVKPVIPSEMFHAGTKFHINPTGMFVIGGPVGD
CGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYAGRYVAKNIVAAGLADKCEIQVSYAIGVAEPTSISINTFNTA
KVDEELIIALVREHFDLRPYGITRMLDLLQPMYKQTAAYGHFGREGSDTAFTWEKTDKVEVLKDAAGL
>Mature_388_residues
MREYAVFTSESVSEGHPDKMADQISDAILDAILKEDPYARVACETLVKTGAVVLAGEITTTANVDFEAIVRQTVNGIGYH
HSDLGFDGSTCAIINMIGKQSPEIAQGVDRQKPEDQGAGDQGLMFGYASRETDVLMPAPISYAHRLMERQAELRRSGALP
WLRPDAKSQVTFAYENGMPVRLDAVVLSTQHDPEISQAQLKEAVIEEIVKPVIPSEMFHAGTKFHINPTGMFVIGGPVGD
CGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYAGRYVAKNIVAAGLADKCEIQVSYAIGVAEPTSISINTFNTA
KVDEELIIALVREHFDLRPYGITRMLDLLQPMYKQTAAYGHFGREGSDTAFTWEKTDKVEVLKDAAGL

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family

Homologues:

Organism=Homo sapiens, GI4557737, Length=380, Percent_Identity=56.3157894736842, Blast_Score=408, Evalue=1e-114,
Organism=Homo sapiens, GI5174529, Length=381, Percent_Identity=55.1181102362205, Blast_Score=408, Evalue=1e-114,
Organism=Escherichia coli, GI1789311, Length=383, Percent_Identity=74.1514360313316, Blast_Score=595, Evalue=1e-171,
Organism=Caenorhabditis elegans, GI17538498, Length=384, Percent_Identity=54.4270833333333, Blast_Score=409, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI25145633, Length=384, Percent_Identity=54.4270833333333, Blast_Score=406, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI17538494, Length=391, Percent_Identity=53.9641943734015, Blast_Score=405, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI17551082, Length=386, Percent_Identity=52.0725388601036, Blast_Score=403, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI32565905, Length=391, Percent_Identity=48.849104859335, Blast_Score=343, Evalue=7e-95,
Organism=Caenorhabditis elegans, GI32565911, Length=338, Percent_Identity=51.4792899408284, Blast_Score=336, Evalue=1e-92,
Organism=Caenorhabditis elegans, GI25145635, Length=338, Percent_Identity=51.4792899408284, Blast_Score=332, Evalue=2e-91,
Organism=Caenorhabditis elegans, GI32565903, Length=319, Percent_Identity=51.0971786833856, Blast_Score=301, Evalue=5e-82,
Organism=Caenorhabditis elegans, GI32565909, Length=83, Percent_Identity=60.2409638554217, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI32565907, Length=44, Percent_Identity=70.4545454545455, Blast_Score=70, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6320710, Length=380, Percent_Identity=55, Blast_Score=404, Evalue=1e-114,
Organism=Saccharomyces cerevisiae, GI6323209, Length=375, Percent_Identity=54.4, Blast_Score=392, Evalue=1e-110,
Organism=Drosophila melanogaster, GI45552159, Length=381, Percent_Identity=55.1181102362205, Blast_Score=397, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24580529, Length=381, Percent_Identity=55.1181102362205, Blast_Score=397, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24580537, Length=381, Percent_Identity=55.1181102362205, Blast_Score=397, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24580533, Length=381, Percent_Identity=55.1181102362205, Blast_Score=397, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24580535, Length=381, Percent_Identity=55.1181102362205, Blast_Score=397, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24580531, Length=381, Percent_Identity=55.1181102362205, Blast_Score=397, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24580527, Length=381, Percent_Identity=55.1181102362205, Blast_Score=394, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24580525, Length=381, Percent_Identity=55.1181102362205, Blast_Score=394, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24580541, Length=369, Percent_Identity=54.20054200542, Blast_Score=373, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24580539, Length=117, Percent_Identity=60.6837606837607, Blast_Score=139, Evalue=3e-33,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): METK_ACIAD (Q6FAQ6)

Other databases:

- EMBL:   CR543861
- RefSeq:   YP_046679.1
- ProteinModelPortal:   Q6FAQ6
- SMR:   Q6FAQ6
- STRING:   Q6FAQ6
- GeneID:   2879491
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD2037
- NMPDR:   fig|62977.3.peg.1941
- eggNOG:   COG0192
- HOGENOM:   HBG443662
- OMA:   IYYAHRL
- PhylomeDB:   Q6FAQ6
- ProtClustDB:   PRK05250
- BioCyc:   ASP62977:ACIAD2037-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00086
- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636
- PANTHER:   PTHR11964
- PIRSF:   PIRSF000497
- TIGRFAMs:   TIGR01034

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N; SSF55973 S-AdoMet_synt

EC number: =2.5.1.6

Molecular weight: Translated: 42057; Mature: 42057

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS00376 ADOMET_SYNTHASE_1; PS00377 ADOMET_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MREYAVFTSESVSEGHPDKMADQISDAILDAILKEDPYARVACETLVKTGAVVLAGEITT
CCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEEECEEE
TANVDFEAIVRQTVNGIGYHHSDLGFDGSTCAIINMIGKQSPEIAQGVDRQKPEDQGAGD
ECCCCHHHHHHHHHHHCCEECCCCCCCCCHHHHHHHHCCCCCHHHHCCCCCCCCCCCCCC
QGLMFGYASRETDVLMPAPISYAHRLMERQAELRRSGALPWLRPDAKSQVTFAYENGMPV
CCEEEEECCCCCCEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCE
RLDAVVLSTQHDPEISQAQLKEAVIEEIVKPVIPSEMFHAGTKFHINPTGMFVIGGPVGD
EEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHCCCEEEECCCEEEEECCCCCC
CGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYAGRYVAKNIVAAGLADKCEIQV
CCCCCCEEEEECCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
SYAIGVAEPTSISINTFNTAKVDEELIIALVREHFDLRPYGITRMLDLLQPMYKQTAAYG
EEEEECCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHC
HFGREGSDTAFTWEKTDKVEVLKDAAGL
CCCCCCCCCEEEECCCCHHHHHHHHCCC
>Mature Secondary Structure
MREYAVFTSESVSEGHPDKMADQISDAILDAILKEDPYARVACETLVKTGAVVLAGEITT
CCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEEECEEE
TANVDFEAIVRQTVNGIGYHHSDLGFDGSTCAIINMIGKQSPEIAQGVDRQKPEDQGAGD
ECCCCHHHHHHHHHHHCCEECCCCCCCCCHHHHHHHHCCCCCHHHHCCCCCCCCCCCCCC
QGLMFGYASRETDVLMPAPISYAHRLMERQAELRRSGALPWLRPDAKSQVTFAYENGMPV
CCEEEEECCCCCCEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCE
RLDAVVLSTQHDPEISQAQLKEAVIEEIVKPVIPSEMFHAGTKFHINPTGMFVIGGPVGD
EEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHCCCEEEECCCEEEEECCCCCC
CGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYAGRYVAKNIVAAGLADKCEIQV
CCCCCCEEEEECCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
SYAIGVAEPTSISINTFNTAKVDEELIIALVREHFDLRPYGITRMLDLLQPMYKQTAAYG
EEEEECCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHC
HFGREGSDTAFTWEKTDKVEVLKDAAGL
CCCCCCCCCEEEECCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA