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The map label for this gene is thiM

Identifier: 50085146

GI number: 50085146

Start: 2002829

End: 2003653

Strand: Direct

Name: thiM

Synonym: ACIAD2011

Alternate gene names: 50085146

Gene position: 2002829-2003653 (Clockwise)

Preceding gene: 50085145

Following gene: 50085150

Centisome position: 55.66

GC content: 43.15

Gene sequence:

>825_bases
ATGTATAACTCATCTAATATTATTGATTACGTGATTGATGCTTGGGAAAAATTACAGGAGCAAGCGCCCCTTGTGCAATG
TATTACCAATAGTGTTGCAGCCAATTATGCAGCTAATGTATTGCTGGCCGCTGGCGCATCACCTGCTATGATTGATAACC
CATTTGAAGCACGAAGTTTCACTGAAATTAGTGGTGCGCTCAGTATTAATGTAGGCACACCGACGACTGAACAAATCCAG
GCAATGCAAATTTCTGCAAAAACAGCACACGAGCATGATATTCCTTGGGTGCTTGACCCCGTTGGTTACGGCCCTATTTT
GAAGTGGCGTAGTGATATGGTGGATGAACTATTACAGTATCATCCTTCTGTGATTCGGGGTAATGCTTCTGAAATTGGTG
CACTGGCAGGCAGTTTAGTACAAAGTAAAGGCGTTGATAGCACTTTAAAAAGTGATGAGATGTTCCAGCTTGCACACAAC
TTACTCACCAAGACAAGCTGTGTCGCCATCTCGGGTGAAACTGACTACATCTTGTCAAATGCAATGACCTGCATTGTAAA
AGTTCGCGGTGGTAGTCACTTACAGCCTAAAATTACCGCCACTGGCTGTGCTTTAGGTACACTCATCGCAGCGTACTGTG
CAGTCGCGCCTGTGCATTTGGCTACAATAGCAGCTCATATTCACTTTGCCATTGCAGGTAAGCTCGCTTATGATCAGGCA
CAGACCATTGGTAGTTTCAATACCATGTTTATGGATTATATCCACATGATGGATGCCAATTTAATTGAACAATATGCTTG
TATCGAGCTAATTTCAGATGATTGA

Upstream 100 bases:

>100_bases
TGATTATGACGCAACAGATTATGTGTCTTTAATTTGCCGCAATTGCAAACATCAAGATCATTATTTTACACAATAATTTT
TCATTCTTTGCAGGGAAGAT

Downstream 100 bases:

>100_bases
AAATCTCAATTCTATATTTTTAAAATATTTAGAGACGATTTTAATCGTCTCTAAATCGGTTTATTTTAGCAGCAAATAAA
TATCGGTGACCAAATCAGCT

Product: hydoxyethylthiazole kinase

Products: NA

Alternate protein names: 4-methyl-5-beta-hydroxyethylthiazole kinase; TH kinase; Thz kinase

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MYNSSNIIDYVIDAWEKLQEQAPLVQCITNSVAANYAANVLLAAGASPAMIDNPFEARSFTEISGALSINVGTPTTEQIQ
AMQISAKTAHEHDIPWVLDPVGYGPILKWRSDMVDELLQYHPSVIRGNASEIGALAGSLVQSKGVDSTLKSDEMFQLAHN
LLTKTSCVAISGETDYILSNAMTCIVKVRGGSHLQPKITATGCALGTLIAAYCAVAPVHLATIAAHIHFAIAGKLAYDQA
QTIGSFNTMFMDYIHMMDANLIEQYACIELISDD

Sequences:

>Translated_274_residues
MYNSSNIIDYVIDAWEKLQEQAPLVQCITNSVAANYAANVLLAAGASPAMIDNPFEARSFTEISGALSINVGTPTTEQIQ
AMQISAKTAHEHDIPWVLDPVGYGPILKWRSDMVDELLQYHPSVIRGNASEIGALAGSLVQSKGVDSTLKSDEMFQLAHN
LLTKTSCVAISGETDYILSNAMTCIVKVRGGSHLQPKITATGCALGTLIAAYCAVAPVHLATIAAHIHFAIAGKLAYDQA
QTIGSFNTMFMDYIHMMDANLIEQYACIELISDD
>Mature_274_residues
MYNSSNIIDYVIDAWEKLQEQAPLVQCITNSVAANYAANVLLAAGASPAMIDNPFEARSFTEISGALSINVGTPTTEQIQ
AMQISAKTAHEHDIPWVLDPVGYGPILKWRSDMVDELLQYHPSVIRGNASEIGALAGSLVQSKGVDSTLKSDEMFQLAHN
LLTKTSCVAISGETDYILSNAMTCIVKVRGGSHLQPKITATGCALGTLIAAYCAVAPVHLATIAAHIHFAIAGKLAYDQA
QTIGSFNTMFMDYIHMMDANLIEQYACIELISDD

Specific function: Thiamine biosynthesis. [C]

COG id: COG2145

COG function: function code H; Hydroxyethylthiazole kinase, sugar kinase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Thz kinase family

Homologues:

Organism=Escherichia coli, GI1788421, Length=239, Percent_Identity=40.1673640167364, Blast_Score=157, Evalue=6e-40,
Organism=Saccharomyces cerevisiae, GI6325042, Length=269, Percent_Identity=28.6245353159851, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIM_ACIAD (Q6FAS9)

Other databases:

- EMBL:   CR543861
- RefSeq:   YP_046656.1
- HSSP:   P39593
- ProteinModelPortal:   Q6FAS9
- SMR:   Q6FAS9
- STRING:   Q6FAS9
- GeneID:   2880748
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD2011
- NMPDR:   fig|62977.3.peg.1918
- eggNOG:   COG2145
- HOGENOM:   HBG351126
- OMA:   AIRGNAG
- PhylomeDB:   Q6FAS9
- ProtClustDB:   CLSK707428
- BioCyc:   ASP62977:ACIAD2011-MONOMER
- HAMAP:   MF_00228
- InterPro:   IPR000417
- PANTHER:   PTHR20857:SF14
- PIRSF:   PIRSF000513
- PRINTS:   PR01099

Pfam domain/function: PF02110 HK

EC number: =2.7.1.50

Molecular weight: Translated: 29467; Mature: 29467

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: NA

Important sites: BINDING 50-50 BINDING 126-126 BINDING 171-171 BINDING 200-200

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYNSSNIIDYVIDAWEKLQEQAPLVQCITNSVAANYAANVLLAAGASPAMIDNPFEARSF
CCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCHH
TEISGALSINVGTPTTEQIQAMQISAKTAHEHDIPWVLDPVGYGPILKWRSDMVDELLQY
HEECCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHH
HPSVIRGNASEIGALAGSLVQSKGVDSTLKSDEMFQLAHNLLTKTSCVAISGETDYILSN
CCHHCCCCHHHHHHHHHHHHHHCCCCCHHCHHHHHHHHHHHHHHHHEEEECCCCHHHHHH
AMTCIVKVRGGSHLQPKITATGCALGTLIAAYCAVAPVHLATIAAHIHFAIAGKLAYDQA
HEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QTIGSFNTMFMDYIHMMDANLIEQYACIELISDD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MYNSSNIIDYVIDAWEKLQEQAPLVQCITNSVAANYAANVLLAAGASPAMIDNPFEARSF
CCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCHH
TEISGALSINVGTPTTEQIQAMQISAKTAHEHDIPWVLDPVGYGPILKWRSDMVDELLQY
HEECCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHH
HPSVIRGNASEIGALAGSLVQSKGVDSTLKSDEMFQLAHNLLTKTSCVAISGETDYILSN
CCHHCCCCHHHHHHHHHHHHHHCCCCCHHCHHHHHHHHHHHHHHHHEEEECCCCHHHHHH
AMTCIVKVRGGSHLQPKITATGCALGTLIAAYCAVAPVHLATIAAHIHFAIAGKLAYDQA
HEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QTIGSFNTMFMDYIHMMDANLIEQYACIELISDD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA