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The map label for this gene is nasD [H]

Identifier: 50085049

GI number: 50085049

Start: 1898213

End: 1899850

Strand: Reverse

Name: nasD [H]

Synonym: ACIAD1909

Alternate gene names: 50085049

Gene position: 1899850-1898213 (Counterclockwise)

Preceding gene: 50085050

Following gene: 50085048

Centisome position: 52.79

GC content: 42.43

Gene sequence:

>1638_bases
ATGAGCAACTTAAAACAGTTAAATCAGATTGCAGAAAATGAATGGATTGATGTCTGTGCCTTGAGCGACATTACTCCAAA
TACGGGTGTCACAGCTTTAATTGATGATCAGCAAATTGCTTTATTTCGTGTCGGGCACGAACAGCGAATCTACGCGCTCA
GCAATCAAGATCCATTCAGTGGGGCAAATGTGATGTCACGTGGAATTATGGGTGATTTGCAAGGAGAACGAGTGATTGCT
TCGCCTATTTATAAACAGCACTTCAGTTTGGCAACGGGGCGTTGTCTGGAAGATAAAGAACAAAAGTTACTGGTTTTCCC
TTGTAAAGTACAATCAGGACGTGTTTGGGTGAGTCCTGTACCTCAAAAAACCTATATTACCAATAACGGATTGGCACAGG
ATAAATTAAAGCTGGTACTGGTAGGAAATGGACTAGCAGGCATGCGCTGTCTAGAAGATTTACTGGATATGAATCCCGAA
CGTTATGAAATCACAGTGATTGGTGAAGAACCGTGGGGGAATTATAACCGTATTATGCTGTCACCTGTTTTATCTGGTGA
AAAAACCATTGAAGATATTATGTTACATCCTCATCAATGGTATCAGGATAAAGCCATTCGCTTTGTGGCGGGCGATCCAG
CGATCAAGATTGATCGTAACCGTAAGCAAGTGCATACCCAAAATGGTGAGTGTGTTGAATATGACCGGCTTATTTTGGCT
ACTGGGTCTAAACCGTTTATTCCACCTATTCAAGGAGCAGACTTAAAAGGTGTCATTAGCTTTCGGGATATCTATGATGT
CAATACCATGGTGGATTACGCATCTCAGAAAAAGCATGCTGTGGTGATTGGTGGTGGCTTATTAGGTCTGGAAGCCGCCT
ATGGTTTAAAACAGCGCGGTATGAATGTGACGGTATTGCATTTGATGGATCGGATTATGGACCGTCAGTTAGATGCTCAA
GCCAGTGTGATGCTACGTCATAGTATTGAGCAAAAAGGTATTGAAATTATAACTGAAGCAAATACCGAGGCTTTGCTGGG
TAATGATCAAGGCCATGTAACGCATTTGCGTTTAAAAGATGGGCGTGTTCTGGATGCCGATTTGGTGGTATTTGCAGTGG
GAATTCGCCCGAATATTCGTCTGGCACAAAGTGCAGGCTTAAGATGTAATCGCGGAGTATTGGTGAATGATACGATGCAA
ACCTTCGACCCAAGTATTTATGCGGTGGGTGAGTGTATAGAACACCGTGGACAAACTTTTGGTTTGGTTGAACCTTTATG
GGGACAAGCATTTATTTGTGCCAGCCATCTGGCCGAACATGGTAGTTTGACTTTTAAAGCCCCAACGGTGCCTACACAGC
TTAAAGTGAGTGGTGTGGATGTGTTTTCGGCAGGTCAAATTGATATTGATGTTCGCCAATCGGAAGATGGTTATGAAGAC
ATTATTATGAATGATGAGAAACGTCAGATTTATAAGCGAATTATTATTCAGCATGATCGTGTCATTGGTGCTGTTTTATT
TGGTGATACCGAAGATGGTACATGGTACGCCGAATTGATCGCAGATCAGACCCCTATTTCTTCAATTCGTAGCAAGCTGC
TGTTTGGTCGAGATTTTGCTTTAAGAAAAGCAGGTTAA

Upstream 100 bases:

>100_bases
ATATCCAGTTTACAGAGGAGCGTGGTCAAATTCGTCCACTTAAAGCCAGTGAACGAGATGAGTCACGTATTCCATTGGTT
GAAGCATAAGGAGAACAAAT

Downstream 100 bases:

>100_bases
GTTAAGATAAAACCGGGTGATCAAGGAGCTTCAATGAATACGATTCCTGCTGTGCAACTCCAAAGTACCACCGATAATCA
GCTTGAATATTCTGTTCAGA

Product: nitrate reductase (electron transfer subunit) and nitrite reductase (small subunit)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 545; Mature: 544

Protein sequence:

>545_residues
MSNLKQLNQIAENEWIDVCALSDITPNTGVTALIDDQQIALFRVGHEQRIYALSNQDPFSGANVMSRGIMGDLQGERVIA
SPIYKQHFSLATGRCLEDKEQKLLVFPCKVQSGRVWVSPVPQKTYITNNGLAQDKLKLVLVGNGLAGMRCLEDLLDMNPE
RYEITVIGEEPWGNYNRIMLSPVLSGEKTIEDIMLHPHQWYQDKAIRFVAGDPAIKIDRNRKQVHTQNGECVEYDRLILA
TGSKPFIPPIQGADLKGVISFRDIYDVNTMVDYASQKKHAVVIGGGLLGLEAAYGLKQRGMNVTVLHLMDRIMDRQLDAQ
ASVMLRHSIEQKGIEIITEANTEALLGNDQGHVTHLRLKDGRVLDADLVVFAVGIRPNIRLAQSAGLRCNRGVLVNDTMQ
TFDPSIYAVGECIEHRGQTFGLVEPLWGQAFICASHLAEHGSLTFKAPTVPTQLKVSGVDVFSAGQIDIDVRQSEDGYED
IIMNDEKRQIYKRIIIQHDRVIGAVLFGDTEDGTWYAELIADQTPISSIRSKLLFGRDFALRKAG

Sequences:

>Translated_545_residues
MSNLKQLNQIAENEWIDVCALSDITPNTGVTALIDDQQIALFRVGHEQRIYALSNQDPFSGANVMSRGIMGDLQGERVIA
SPIYKQHFSLATGRCLEDKEQKLLVFPCKVQSGRVWVSPVPQKTYITNNGLAQDKLKLVLVGNGLAGMRCLEDLLDMNPE
RYEITVIGEEPWGNYNRIMLSPVLSGEKTIEDIMLHPHQWYQDKAIRFVAGDPAIKIDRNRKQVHTQNGECVEYDRLILA
TGSKPFIPPIQGADLKGVISFRDIYDVNTMVDYASQKKHAVVIGGGLLGLEAAYGLKQRGMNVTVLHLMDRIMDRQLDAQ
ASVMLRHSIEQKGIEIITEANTEALLGNDQGHVTHLRLKDGRVLDADLVVFAVGIRPNIRLAQSAGLRCNRGVLVNDTMQ
TFDPSIYAVGECIEHRGQTFGLVEPLWGQAFICASHLAEHGSLTFKAPTVPTQLKVSGVDVFSAGQIDIDVRQSEDGYED
IIMNDEKRQIYKRIIIQHDRVIGAVLFGDTEDGTWYAELIADQTPISSIRSKLLFGRDFALRKAG
>Mature_544_residues
SNLKQLNQIAENEWIDVCALSDITPNTGVTALIDDQQIALFRVGHEQRIYALSNQDPFSGANVMSRGIMGDLQGERVIAS
PIYKQHFSLATGRCLEDKEQKLLVFPCKVQSGRVWVSPVPQKTYITNNGLAQDKLKLVLVGNGLAGMRCLEDLLDMNPER
YEITVIGEEPWGNYNRIMLSPVLSGEKTIEDIMLHPHQWYQDKAIRFVAGDPAIKIDRNRKQVHTQNGECVEYDRLILAT
GSKPFIPPIQGADLKGVISFRDIYDVNTMVDYASQKKHAVVIGGGLLGLEAAYGLKQRGMNVTVLHLMDRIMDRQLDAQA
SVMLRHSIEQKGIEIITEANTEALLGNDQGHVTHLRLKDGRVLDADLVVFAVGIRPNIRLAQSAGLRCNRGVLVNDTMQT
FDPSIYAVGECIEHRGQTFGLVEPLWGQAFICASHLAEHGSLTFKAPTVPTQLKVSGVDVFSAGQIDIDVRQSEDGYEDI
IMNDEKRQIYKRIIIQHDRVIGAVLFGDTEDGTWYAELIADQTPISSIRSKLLFGRDFALRKAG

Specific function: Required for nitrite assimilation [H]

COG id: COG1251

COG function: function code C; NAD(P)H-nitrite reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Homo sapiens, GI65787454, Length=384, Percent_Identity=25.5208333333333, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI21389617, Length=384, Percent_Identity=25.5208333333333, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI226437568, Length=384, Percent_Identity=25.5208333333333, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI4757732, Length=227, Percent_Identity=27.3127753303965, Blast_Score=78, Evalue=3e-14,
Organism=Homo sapiens, GI22202629, Length=227, Percent_Identity=27.3127753303965, Blast_Score=77, Evalue=3e-14,
Organism=Escherichia coli, GI1789765, Length=383, Percent_Identity=38.1201044386423, Blast_Score=224, Evalue=1e-59,
Organism=Escherichia coli, GI1789065, Length=343, Percent_Identity=25.9475218658892, Blast_Score=130, Evalue=3e-31,
Organism=Escherichia coli, GI1788892, Length=237, Percent_Identity=27.4261603375527, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1789766, Length=103, Percent_Identity=42.7184466019417, Blast_Score=102, Evalue=7e-23,
Organism=Escherichia coli, GI87082354, Length=229, Percent_Identity=29.6943231441048, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1789915, Length=189, Percent_Identity=29.6296296296296, Blast_Score=62, Evalue=9e-11,
Organism=Caenorhabditis elegans, GI17559934, Length=408, Percent_Identity=26.9607843137255, Blast_Score=127, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI71982866, Length=167, Percent_Identity=26.9461077844311, Blast_Score=65, Evalue=7e-11,
Organism=Drosophila melanogaster, GI281359715, Length=280, Percent_Identity=26.7857142857143, Blast_Score=113, Evalue=4e-25,
Organism=Drosophila melanogaster, GI281359713, Length=280, Percent_Identity=26.7857142857143, Blast_Score=113, Evalue=4e-25,
Organism=Drosophila melanogaster, GI24639250, Length=280, Percent_Identity=26.7857142857143, Blast_Score=113, Evalue=4e-25,
Organism=Drosophila melanogaster, GI18543267, Length=280, Percent_Identity=26.7857142857143, Blast_Score=113, Evalue=4e-25,
Organism=Drosophila melanogaster, GI24639252, Length=280, Percent_Identity=26.7857142857143, Blast_Score=113, Evalue=4e-25,
Organism=Drosophila melanogaster, GI24639257, Length=282, Percent_Identity=26.241134751773, Blast_Score=113, Evalue=4e-25,
Organism=Drosophila melanogaster, GI24585130, Length=281, Percent_Identity=25.9786476868327, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI21358499, Length=221, Percent_Identity=28.0542986425339, Blast_Score=67, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007419
- InterPro:   IPR013027
- InterPro:   IPR005117
- InterPro:   IPR012744
- InterPro:   IPR017121
- InterPro:   IPR006067
- InterPro:   IPR006066
- InterPro:   IPR001327 [H]

Pfam domain/function: PF04324 Fer2_BFD; PF01077 NIR_SIR; PF03460 NIR_SIR_ferr; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.7.1.4 [H]

Molecular weight: Translated: 60643; Mature: 60512

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNLKQLNQIAENEWIDVCALSDITPNTGVTALIDDQQIALFRVGHEQRIYALSNQDPFS
CCCHHHHHHHHCCCCEEEEEECCCCCCCCEEEEECCCEEEEEEECCCCEEEEECCCCCCC
GANVMSRGIMGDLQGERVIASPIYKQHFSLATGRCLEDKEQKLLVFPCKVQSGRVWVSPV
CHHHHHCCCCCCCCCCEEEECHHHHHHHHHHCCCCCCCCCCEEEEEEEEECCCEEEECCC
PQKTYITNNGLAQDKLKLVLVGNGLAGMRCLEDLLDMNPERYEITVIGEEPWGNYNRIML
CCEEEEECCCCCCCCEEEEEEECCCHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCEEEE
SPVLSGEKTIEDIMLHPHQWYQDKAIRFVAGDPAIKIDRNRKQVHTQNGECVEYDRLILA
EECCCCCHHHHHHHCCCCCCCCCCEEEEEECCCEEEEECCCCEEECCCCCEEEECEEEEE
TGSKPFIPPIQGADLKGVISFRDIYDVNTMVDYASQKKHAVVIGGGLLGLEAAYGLKQRG
ECCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHCC
MNVTVLHLMDRIMDRQLDAQASVMLRHSIEQKGIEIITEANTEALLGNDQGHVTHLRLKD
CCEEHHHHHHHHHHHHCCHHHHHHHHHHHHHCCEEEEEECCCEEEECCCCCCEEEEEECC
GRVLDADLVVFAVGIRPNIRLAQSAGLRCNRGVLVNDTMQTFDPSIYAVGECIEHRGQTF
CCEEECCEEEEEECCCCCEEEHHCCCCEECCCEEEECCHHHHCCCHHHHHHHHHHCCCEE
GLVEPLWGQAFICASHLAEHGSLTFKAPTVPTQLKVSGVDVFSAGQIDIDVRQSEDGYED
CCCCCCCCCHHHHHHHHHHCCCEEEECCCCCCEEEEECEEEEECCEEEEEEECCCCCCHH
IIMNDEKRQIYKRIIIQHDRVIGAVLFGDTEDGTWYAELIADQTPISSIRSKLLFGRDFA
HEECCHHHHHHHHHHHHCCCEEEEEEECCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCE
LRKAG
EECCC
>Mature Secondary Structure 
SNLKQLNQIAENEWIDVCALSDITPNTGVTALIDDQQIALFRVGHEQRIYALSNQDPFS
CCHHHHHHHHCCCCEEEEEECCCCCCCCEEEEECCCEEEEEEECCCCEEEEECCCCCCC
GANVMSRGIMGDLQGERVIASPIYKQHFSLATGRCLEDKEQKLLVFPCKVQSGRVWVSPV
CHHHHHCCCCCCCCCCEEEECHHHHHHHHHHCCCCCCCCCCEEEEEEEEECCCEEEECCC
PQKTYITNNGLAQDKLKLVLVGNGLAGMRCLEDLLDMNPERYEITVIGEEPWGNYNRIML
CCEEEEECCCCCCCCEEEEEEECCCHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCEEEE
SPVLSGEKTIEDIMLHPHQWYQDKAIRFVAGDPAIKIDRNRKQVHTQNGECVEYDRLILA
EECCCCCHHHHHHHCCCCCCCCCCEEEEEECCCEEEEECCCCEEECCCCCEEEECEEEEE
TGSKPFIPPIQGADLKGVISFRDIYDVNTMVDYASQKKHAVVIGGGLLGLEAAYGLKQRG
ECCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHCC
MNVTVLHLMDRIMDRQLDAQASVMLRHSIEQKGIEIITEANTEALLGNDQGHVTHLRLKD
CCEEHHHHHHHHHHHHCCHHHHHHHHHHHHHCCEEEEEECCCEEEECCCCCCEEEEEECC
GRVLDADLVVFAVGIRPNIRLAQSAGLRCNRGVLVNDTMQTFDPSIYAVGECIEHRGQTF
CCEEECCEEEEEECCCCCEEEHHCCCCEECCCEEEECCHHHHCCCHHHHHHHHHHCCCEE
GLVEPLWGQAFICASHLAEHGSLTFKAPTVPTQLKVSGVDVFSAGQIDIDVRQSEDGYED
CCCCCCCCCHHHHHHHHHHCCCEEEECCCCCCEEEEECEEEEECCEEEEEEECCCCCCHH
IIMNDEKRQIYKRIIIQHDRVIGAVLFGDTEDGTWYAELIADQTPISSIRSKLLFGRDFA
HEECCHHHHHHHHHHHHCCCEEEEEEECCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCE
LRKAG
EECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7868621; 8969502; 9384377 [H]