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The map label for this gene is otsA [H]

Identifier: 50084925

GI number: 50084925

Start: 1780468

End: 1781904

Strand: Reverse

Name: otsA [H]

Synonym: ACIAD1774

Alternate gene names: 50084925

Gene position: 1781904-1780468 (Counterclockwise)

Preceding gene: 50084926

Following gene: 50084923

Centisome position: 49.52

GC content: 35.35

Gene sequence:

>1437_bases
ATGTCTAGACTTATTATTTTATCCAATCGTGTGAGTTTACCTCAGACTGAATGTGTAGCAGGTGGATTAGCTGTTGCCAT
GCAAGATGCGTTAAGTCAGCTTGGTGGTGTTTGGGTTGGTTGGAATGGCAAAAAAATTGAACAACCATTTGAACAGCATT
TTGATATTTTATACAAATCCAAAATTACTTATATGACCTGTGCACTGACACAGCAACAGTATCAGGATTATTACTGTGGT
TTTTCAAATAATACATTATGGCCAGCAATGCATGACCGTGAAGACCTGATCGAGTATAACCCTAAAGAATTCAAAACTTA
TCAAGATGTGAACCGTTTATTTGCCACCAAAATTGCAGAATTTGCCCAACCAGATGATCTAATCTGGGTACAGGATTATC
ATTTTCTAAGTGTTGCTCGCCATTGTCGTGAGCTGGGAATGCAAAATAAAATCGGATTCTTTCTTCATATTCCTTTTGCC
TCACTGGAAATATGGAGAAAGATTCCTTGTGCAGAGCAGCTTATGGATGATCTATGTCATTATAATTTAATAGGTTTGCA
AACTGCTCAAGATCAGGAAAACTGTATTTCAGTATGTAGTGTACTTTTAAAAGCTCAGAAAATTCAAAACAATATGATTT
CATTGAATCAAAGAGTGACGCAAATTCAAGACTATCCAATTGGTGTTGATGTTAAAGGTATTCAAAAAACACTCTCAAAT
TTATCTGATCAAGATCAATATTTGACAAACCCCTCTGCTATCAAAACAATTATTGGGGTCGATCGAATCGATTATTCAAA
AGGTTTATTCGAAAGATTTAATGCCTTTGCCGAGTTTTTAGATCGCAACACAGCGTTTCATCAAAAAGTTCAACATGTGC
AAATAGCTTCACCTTGCCGTCTGGAAGTTGCTACATATAAGCGACTATTTGAACGGTTTAAGTTGAAAGTAGAATTAATC
AATAGTGAATTTTCCCAAGATAACTGGCTTCCAATTGTTTGTCATTATGATTCAAAACCACATCACGAACTTATGCAATT
TTATCGTAATGCGGATATCTGCTGGGTTACTTCACTTAAAGATGGTATGAATTTAGTTGCTAAAGAATATATTGCTGCAC
AAAATCCTGATAACCCTGGCGTACTCATCTTATCTGAATTTGCAGGGGCGGCAGAACAAATGCAAGATGCTCTAATCGTT
AATCCCAAGAACCGTGATGCAATGATTAAAGCCTTGAAGCTTGCGTTAAATATGTCTTTGGAGGAGCGTCAATATCGTTA
TCATAAACTTATAAAACAACTTATAGAATATGATATTAATCAGTGGCGAGATGATTTTCTGAAAGACTTGAATTCCTCAC
AATACATTCAAAACTTCGACTATAATCATCGTTTATCAACTGTATTGTTTTATCCAAATTATTCTTCCAGAATCTAA

Upstream 100 bases:

>100_bases
AGATTGAAAAATATTGAGCACGTCGAAGAATTTTTAAACTTGCTCTCTCATCATTTTCCAGATTCATCTACTCAAATTAC
TAAAAAATATGGAGAACAAC

Downstream 100 bases:

>100_bases
TTGTTATTATGCTTCATCGCTCATATTTTTCATAAGCGATGAAGCTATTCATATAAAGATATCTATTATTTTACTTGTAA
CTGACTAAAATAATTGAGCA

Product: trehalose-6-phosphate synthase

Products: NA

Alternate protein names: Osmoregulatory trehalose synthesis protein A; Trehalose-6-phosphate synthase; UDP-glucose-glucosephosphate glucosyltransferase [H]

Number of amino acids: Translated: 478; Mature: 477

Protein sequence:

>478_residues
MSRLIILSNRVSLPQTECVAGGLAVAMQDALSQLGGVWVGWNGKKIEQPFEQHFDILYKSKITYMTCALTQQQYQDYYCG
FSNNTLWPAMHDREDLIEYNPKEFKTYQDVNRLFATKIAEFAQPDDLIWVQDYHFLSVARHCRELGMQNKIGFFLHIPFA
SLEIWRKIPCAEQLMDDLCHYNLIGLQTAQDQENCISVCSVLLKAQKIQNNMISLNQRVTQIQDYPIGVDVKGIQKTLSN
LSDQDQYLTNPSAIKTIIGVDRIDYSKGLFERFNAFAEFLDRNTAFHQKVQHVQIASPCRLEVATYKRLFERFKLKVELI
NSEFSQDNWLPIVCHYDSKPHHELMQFYRNADICWVTSLKDGMNLVAKEYIAAQNPDNPGVLILSEFAGAAEQMQDALIV
NPKNRDAMIKALKLALNMSLEERQYRYHKLIKQLIEYDINQWRDDFLKDLNSSQYIQNFDYNHRLSTVLFYPNYSSRI

Sequences:

>Translated_478_residues
MSRLIILSNRVSLPQTECVAGGLAVAMQDALSQLGGVWVGWNGKKIEQPFEQHFDILYKSKITYMTCALTQQQYQDYYCG
FSNNTLWPAMHDREDLIEYNPKEFKTYQDVNRLFATKIAEFAQPDDLIWVQDYHFLSVARHCRELGMQNKIGFFLHIPFA
SLEIWRKIPCAEQLMDDLCHYNLIGLQTAQDQENCISVCSVLLKAQKIQNNMISLNQRVTQIQDYPIGVDVKGIQKTLSN
LSDQDQYLTNPSAIKTIIGVDRIDYSKGLFERFNAFAEFLDRNTAFHQKVQHVQIASPCRLEVATYKRLFERFKLKVELI
NSEFSQDNWLPIVCHYDSKPHHELMQFYRNADICWVTSLKDGMNLVAKEYIAAQNPDNPGVLILSEFAGAAEQMQDALIV
NPKNRDAMIKALKLALNMSLEERQYRYHKLIKQLIEYDINQWRDDFLKDLNSSQYIQNFDYNHRLSTVLFYPNYSSRI
>Mature_477_residues
SRLIILSNRVSLPQTECVAGGLAVAMQDALSQLGGVWVGWNGKKIEQPFEQHFDILYKSKITYMTCALTQQQYQDYYCGF
SNNTLWPAMHDREDLIEYNPKEFKTYQDVNRLFATKIAEFAQPDDLIWVQDYHFLSVARHCRELGMQNKIGFFLHIPFAS
LEIWRKIPCAEQLMDDLCHYNLIGLQTAQDQENCISVCSVLLKAQKIQNNMISLNQRVTQIQDYPIGVDVKGIQKTLSNL
SDQDQYLTNPSAIKTIIGVDRIDYSKGLFERFNAFAEFLDRNTAFHQKVQHVQIASPCRLEVATYKRLFERFKLKVELIN
SEFSQDNWLPIVCHYDSKPHHELMQFYRNADICWVTSLKDGMNLVAKEYIAAQNPDNPGVLILSEFAGAAEQMQDALIVN
PKNRDAMIKALKLALNMSLEERQYRYHKLIKQLIEYDINQWRDDFLKDLNSSQYIQNFDYNHRLSTVLFYPNYSSRI

Specific function: Catalyzes the transfer of glucose from UDP-glucose to glucose-6-phosphate to form alpha,alpha-1,1 trehalose-6-phosphate. Acts with retention of the anomeric configuration of the UDP-sugar donor [H]

COG id: COG0380

COG function: function code G; Trehalose-6-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 20 family [H]

Homologues:

Organism=Escherichia coli, GI1788206, Length=459, Percent_Identity=39.4335511982571, Blast_Score=332, Evalue=4e-92,
Organism=Caenorhabditis elegans, GI115533560, Length=421, Percent_Identity=27.5534441805226, Blast_Score=143, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI115533558, Length=421, Percent_Identity=27.5534441805226, Blast_Score=143, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI71987012, Length=363, Percent_Identity=29.7520661157025, Blast_Score=129, Evalue=5e-30,
Organism=Saccharomyces cerevisiae, GI6319602, Length=467, Percent_Identity=31.6916488222698, Blast_Score=236, Evalue=7e-63,
Organism=Saccharomyces cerevisiae, GI6320279, Length=423, Percent_Identity=29.0780141843972, Blast_Score=155, Evalue=1e-38,
Organism=Saccharomyces cerevisiae, GI6323917, Length=453, Percent_Identity=26.9315673289183, Blast_Score=154, Evalue=4e-38,
Organism=Saccharomyces cerevisiae, GI6323537, Length=417, Percent_Identity=27.0983213429257, Blast_Score=149, Evalue=7e-37,
Organism=Drosophila melanogaster, GI19920676, Length=525, Percent_Identity=29.9047619047619, Blast_Score=247, Evalue=1e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001830
- InterPro:   IPR012766 [H]

Pfam domain/function: PF00982 Glyco_transf_20 [H]

EC number: =2.4.1.15 [H]

Molecular weight: Translated: 55659; Mature: 55528

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: PS00018 EF_HAND_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCEEEEEEECCCCCCCC
>Mature Secondary Structure 
SRLIILSNRVSLPQTECVAGGLAVAMQDALSQLGGVWVGWNGKKIEQPFEQHFDILYKS
CCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCEEECCCCCHHHHHHHHHHHHHHHC
KITYMTCALTQQQYQDYYCGFSNNTLWPAMHDREDLIEYNPKEFKTYQDVNRLFATKIAE
CHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHH
FAQPDDLIWVQDYHFLSVARHCRELGMQNKIGFFLHIPFASLEIWRKIPCAEQLMDDLCH
HCCCCCEEEECCHHHHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHCCHHHHHHHHHHC
YNLIGLQTAQDQENCISVCSVLLKAQKIQNNMISLNQRVTQIQDYPIGVDVKGIQKTLSN
CCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHC
LSDQDQYLTNPSAIKTIIGVDRIDYSKGLFERFNAFAEFLDRNTAFHQKVQHVQIASPCR
CCCCHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHEECCCCH
LEVATYKRLFERFKLKVELINSEFSQDNWLPIVCHYDSKPHHELMQFYRNADICWVTSLK
HHHHHHHHHHHHHHHHHEEECCCCCCCCCEEEEEECCCCCHHHHHHHHCCCCEEEEECCH
DGMNLVAKEYIAAQNPDNPGVLILSEFAGAAEQMQDALIVNPKNRDAMIKALKLALNMSL
HHHHHHHHHHHHCCCCCCCCEEEEHHHCCHHHHHCCCEEECCCCHHHHHHHHHHHHCCCH
EERQYRYHKLIKQLIEYDINQWRDDFLKDLNSSQYIQNFDYNHRLSTVLFYPNYSSRI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCEEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA