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The map label for this gene is braD [H]

Identifier: 50084917

GI number: 50084917

Start: 1773033

End: 1774679

Strand: Direct

Name: braD [H]

Synonym: ACIAD1766

Alternate gene names: 50084917

Gene position: 1773033-1774679 (Clockwise)

Preceding gene: 50084916

Following gene: 50084918

Centisome position: 49.27

GC content: 40.32

Gene sequence:

>1647_bases
ATGATGATCAAAGATTATCGGATCTGGGTGGTGTTGTGTTTAGTACTACAATTAGGGTGCATTTCTGCGTGGGCAGCAGA
AGAAACCCATCCTGTAGCTGATAATACACCTGCCATTCATAGCCTACAGCAAGAAGATGCGATTCAACAGTTTGTAAAAT
CTGATTTTAATACTCGTCGAATGATGCTCAATCAATGGCCTGCAAGTATTGAAATACTTGATAAACTTGTGACTTATATT
GATGAAAATGAGTTATATACAGATAGCGCAGGACATACCTATCTATTAAAAAATGATGAGACTTTACTGAGTTATCCAGA
TGAAAAAATTATAAAAGACTGGCCCGCAGATTTATCACAAGTCACTTTAGTCAATACATTGCGTAAAGCCTTAACTTTTG
GTCAAGCAAAAGCCAAGCTGAACTCTGAAGATGCGACCCAACGATTACGTGCAGTCGATATTTTAGAAAATAATATTTCT
GAGCTAGATACTCAATTTATCCGACAGCTTTACTTAAAAGAAACCAATAACACAGTAAAAGCACGTCTTGCACAGCTTAA
AGCACGACTCGATTTTGATAGTTCTGATATTTTAGTCAAAATCGATGCTGTAAAAGTGTTATCGGAATCGAATCGTCCTG
ATGTACTTGCGTTAATGGAACAAGAATTACAACAACCACAATTAAACCCAGAGCTTAAAGCAACCTTACTTGATGCAAAA
AATAAAATCAAAACACGCATACAAATCAGTGAGTGGTCTGGTCATGTCTTTTCAGGGTTAAGTACAGCCAGTATTTTGCT
GCTAGCAGCACTTGGACTTGCAATTACTTATGGGTTATTAGGCGTAATTAATATGGCGCATGGTGAGCTGATTATGATTG
GTGCCTATACCACATATGTCGTGCAAAGCCTGTTTAAAGCTCATCTTGCTCACTGGGTAGATTGGTATTTGCTGATTGCC
ATTCCAGCCGCATTTATCGTGAGTGCACTCATTGGCATGCTGATCGAACGCACGATTATACGGCCGCTGTATGGTCGTCA
ATTAGAAACGTTACTTGCAACTTTTGGTGTAAGTCTAATTTTGATGCAAGCCGTACGTATGGTTTTTGGTGCTCAAAACG
TGGAAGTATCAAATATCACATGGTTAAGCGGCGGTATAGCACTGACGCCAAGCCTTATGCTGCCTTATAACCGTATTGCG
ATTATCGTGTTTACAGTCACTATTTTGCTGCTATTGGTATTTTTACTCAATAAAACACGTTTTGGTTTATTCATTCGCGC
CGTTACCCAAAATCGTCAAATGGCAAGAGCGGTTGGAATTCGTTCGGCACGTATCGATATGATGGCTTTTGGTCTGGGAT
CAGGCCTTGCTGGACTGGCAGGCTGTGCTTTGGCGCAAGTGGGTAATGTTGGGCCAGATCTAGGTCAAAACTATATTATC
GATGCTTTTCTGGTGGTGGTCGTGGGTGGTGTAGGTCAAGTCTGGGGTGCTGTATTGGCAGCATTGGGTTTGGGCGTAAG
TGGAACAATTCTAGAAATTGGTTTAGGTGCAGTATTGGCCAAAATTATTCTGTTGGTTCTTGTGATTTTGTTTATTCAAA
AACGTCCACAAGGTTTATTTGCAATCAAAGGCCGTTTTGTGGAGTAA

Upstream 100 bases:

>100_bases
TCCTTACATTCCAAAATAATGAATGAATCTCACCCATTTCAAACGGAATGGGTGATTCCCCATATTTTGATGAAAATATT
CAACTGATTAAGGTTCCAAT

Downstream 100 bases:

>100_bases
ATAACAATGAAGATCGCCACTTTGTTTTCTGATAAACCCTATAGTGCTGTACTCATAGCGCTATGTTTTCTGGTTGTTAT
GATTTTGCCATGGCTACATC

Product: branched chain amino acid ABC transporter permease

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 548; Mature: 548

Protein sequence:

>548_residues
MMIKDYRIWVVLCLVLQLGCISAWAAEETHPVADNTPAIHSLQQEDAIQQFVKSDFNTRRMMLNQWPASIEILDKLVTYI
DENELYTDSAGHTYLLKNDETLLSYPDEKIIKDWPADLSQVTLVNTLRKALTFGQAKAKLNSEDATQRLRAVDILENNIS
ELDTQFIRQLYLKETNNTVKARLAQLKARLDFDSSDILVKIDAVKVLSESNRPDVLALMEQELQQPQLNPELKATLLDAK
NKIKTRIQISEWSGHVFSGLSTASILLLAALGLAITYGLLGVINMAHGELIMIGAYTTYVVQSLFKAHLAHWVDWYLLIA
IPAAFIVSALIGMLIERTIIRPLYGRQLETLLATFGVSLILMQAVRMVFGAQNVEVSNITWLSGGIALTPSLMLPYNRIA
IIVFTVTILLLLVFLLNKTRFGLFIRAVTQNRQMARAVGIRSARIDMMAFGLGSGLAGLAGCALAQVGNVGPDLGQNYII
DAFLVVVVGGVGQVWGAVLAALGLGVSGTILEIGLGAVLAKIILLVLVILFIQKRPQGLFAIKGRFVE

Sequences:

>Translated_548_residues
MMIKDYRIWVVLCLVLQLGCISAWAAEETHPVADNTPAIHSLQQEDAIQQFVKSDFNTRRMMLNQWPASIEILDKLVTYI
DENELYTDSAGHTYLLKNDETLLSYPDEKIIKDWPADLSQVTLVNTLRKALTFGQAKAKLNSEDATQRLRAVDILENNIS
ELDTQFIRQLYLKETNNTVKARLAQLKARLDFDSSDILVKIDAVKVLSESNRPDVLALMEQELQQPQLNPELKATLLDAK
NKIKTRIQISEWSGHVFSGLSTASILLLAALGLAITYGLLGVINMAHGELIMIGAYTTYVVQSLFKAHLAHWVDWYLLIA
IPAAFIVSALIGMLIERTIIRPLYGRQLETLLATFGVSLILMQAVRMVFGAQNVEVSNITWLSGGIALTPSLMLPYNRIA
IIVFTVTILLLLVFLLNKTRFGLFIRAVTQNRQMARAVGIRSARIDMMAFGLGSGLAGLAGCALAQVGNVGPDLGQNYII
DAFLVVVVGGVGQVWGAVLAALGLGVSGTILEIGLGAVLAKIILLVLVILFIQKRPQGLFAIKGRFVE
>Mature_548_residues
MMIKDYRIWVVLCLVLQLGCISAWAAEETHPVADNTPAIHSLQQEDAIQQFVKSDFNTRRMMLNQWPASIEILDKLVTYI
DENELYTDSAGHTYLLKNDETLLSYPDEKIIKDWPADLSQVTLVNTLRKALTFGQAKAKLNSEDATQRLRAVDILENNIS
ELDTQFIRQLYLKETNNTVKARLAQLKARLDFDSSDILVKIDAVKVLSESNRPDVLALMEQELQQPQLNPELKATLLDAK
NKIKTRIQISEWSGHVFSGLSTASILLLAALGLAITYGLLGVINMAHGELIMIGAYTTYVVQSLFKAHLAHWVDWYLLIA
IPAAFIVSALIGMLIERTIIRPLYGRQLETLLATFGVSLILMQAVRMVFGAQNVEVSNITWLSGGIALTPSLMLPYNRIA
IIVFTVTILLLLVFLLNKTRFGLFIRAVTQNRQMARAVGIRSARIDMMAFGLGSGLAGLAGCALAQVGNVGPDLGQNYII
DAFLVVVVGGVGQVWGAVLAALGLGVSGTILEIGLGAVLAKIILLVLVILFIQKRPQGLFAIKGRFVE

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0559

COG function: function code E; Branched-chain amino acid ABC-type transport system, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789866, Length=295, Percent_Identity=28.135593220339, Blast_Score=88, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851 [H]

Pfam domain/function: PF02653 BPD_transp_2 [H]

EC number: NA

Molecular weight: Translated: 60350; Mature: 60350

Theoretical pI: Translated: 7.62; Mature: 7.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMIKDYRIWVVLCLVLQLGCISAWAAEETHPVADNTPAIHSLQQEDAIQQFVKSDFNTRR
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHH
MMLNQWPASIEILDKLVTYIDENELYTDSAGHTYLLKNDETLLSYPDEKIIKDWPADLSQ
HHHHCCCHHHHHHHHHHHHHCCCCEEECCCCCEEEEECCCHHCCCCCHHHHHHCCCCHHH
VTLVNTLRKALTFGQAKAKLNSEDATQRLRAVDILENNISELDTQFIRQLYLKETNNTVK
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
ARLAQLKARLDFDSSDILVKIDAVKVLSESNRPDVLALMEQELQQPQLNPELKATLLDAK
HHHHHHHHHCCCCCCCEEEEEHHHHHHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHH
NKIKTRIQISEWSGHVFSGLSTASILLLAALGLAITYGLLGVINMAHGELIMIGAYTTYV
HHHHEEEEEECCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHH
VQSLFKAHLAHWVDWYLLIAIPAAFIVSALIGMLIERTIIRPLYGRQLETLLATFGVSLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LMQAVRMVFGAQNVEVSNITWLSGGIALTPSLMLPYNRIAIIVFTVTILLLLVFLLNKTR
HHHHHHHHHCCCCCEECEEEEECCCCEECCHHHCCHHHHHHHHHHHHHHHHHHHHHCCCH
FGLFIRAVTQNRQMARAVGIRSARIDMMAFGLGSGLAGLAGCALAQVGNVGPDLGQNYII
HHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCHHHH
DAFLVVVVGGVGQVWGAVLAALGLGVSGTILEIGLGAVLAKIILLVLVILFIQKRPQGLF
HHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
AIKGRFVE
EECCCCCC
>Mature Secondary Structure
MMIKDYRIWVVLCLVLQLGCISAWAAEETHPVADNTPAIHSLQQEDAIQQFVKSDFNTRR
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHH
MMLNQWPASIEILDKLVTYIDENELYTDSAGHTYLLKNDETLLSYPDEKIIKDWPADLSQ
HHHHCCCHHHHHHHHHHHHHCCCCEEECCCCCEEEEECCCHHCCCCCHHHHHHCCCCHHH
VTLVNTLRKALTFGQAKAKLNSEDATQRLRAVDILENNISELDTQFIRQLYLKETNNTVK
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
ARLAQLKARLDFDSSDILVKIDAVKVLSESNRPDVLALMEQELQQPQLNPELKATLLDAK
HHHHHHHHHCCCCCCCEEEEEHHHHHHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHH
NKIKTRIQISEWSGHVFSGLSTASILLLAALGLAITYGLLGVINMAHGELIMIGAYTTYV
HHHHEEEEEECCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHH
VQSLFKAHLAHWVDWYLLIAIPAAFIVSALIGMLIERTIIRPLYGRQLETLLATFGVSLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LMQAVRMVFGAQNVEVSNITWLSGGIALTPSLMLPYNRIAIIVFTVTILLLLVFLLNKTR
HHHHHHHHHCCCCCEECEEEEECCCCEECCHHHCCHHHHHHHHHHHHHHHHHHHHHCCCH
FGLFIRAVTQNRQMARAVGIRSARIDMMAFGLGSGLAGLAGCALAQVGNVGPDLGQNYII
HHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCHHHH
DAFLVVVVGGVGQVWGAVLAALGLGVSGTILEIGLGAVLAKIILLVLVILFIQKRPQGLF
HHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
AIKGRFVE
EECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043; 2509433 [H]