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The map label for this gene is amiC [H]

Identifier: 50084916

GI number: 50084916

Start: 1771665

End: 1772951

Strand: Direct

Name: amiC [H]

Synonym: ACIAD1765

Alternate gene names: 50084916

Gene position: 1771665-1772951 (Clockwise)

Preceding gene: 50084915

Following gene: 50084917

Centisome position: 49.23

GC content: 41.65

Gene sequence:

>1287_bases
ATGTTTCAACAAAAAATGATGTCAATTTCATTATGTGTAGCTGCAATCATGGGAAGTGCAGGCCTTGTGGGGTGTTCTAA
ATCTTCCGACAAACCAGCAGAGCAAGCTAAAGCTGCTTCAGCTCCTGTCGCCCAAAGTGGAGATACAATTAAAGTCGGAA
TTTTGCATTCACTTTCAGGAACGATGGCCATTTCTGAAACATCGTTAAAAGATACTGCATTAATGACGATTAATGAGATT
AACGCGAAAGGCGGTGTCTTAGGCAAAAAGCTTGAACCTGTGATTGTAGATCCTGCTTCAGATTGGCCACTTTTTGCTGA
AAAAGCACGTCAGCTCATCACACAAGATAAAGTGGCTGTGATTTTTGGATGCTGGACATCCGTTTCGCGTAAATCTGTAC
TGCCTGTGGTTGAAGAATTAAACGGTTTGTTGTTTTATCCAGTACAGTATGAAGGCCAGGAACAATCCAAAAATATTTTC
TATACGGGTGCTGCACCAAACCAGCAAGCGATTCCTGCTGTCGAGTATTTAATGAGTGACGAAGGAGGTAAAGCTCAACG
CTTTGTATTACTGGGAACAGATTATGTTTATCCACGCACCACCAACAAAATCTTACGCGCTTTCTTGAAATCTAAAGGCG
TAGCCGATGCCGACATCATGGAAGAATATACGCCATTTGGACATAATAATTATCAGACCATTGTGGCAAACATTAAAAAA
TTCTCGGCTGGTAAAAAAACCGCAGTAATCTCGACTATTAATGGTGATTCGAACGTTCCGTTCTATCGTGAACTGGGCAA
TCAGGGCATTAAAGCATCCGATATTCCAGTGATGGCATTTTCGGTAGGCGAAGAAGAGTTACGTGGTATTGATACCAAAC
CACTGGTTGGACATTTGGCAGCATGGAATTATTTTATGTCAGTCAAAAATCCTAAAAATACTGATTTCATTAATAAATTT
AAGCAATATGCGGTCGAGTACAAATTACCAAATGCCAATAAGGTTGTGACCAACGATCCGATGGAAGCGACCTATGTCGG
CATCAATATGTGGAAGCAAGCTGTTGAGAAAGCAGGTAGCACCGATGTTGATAAAGTGCGTACTGCGATGGCAGGTCAAA
CCTTCGCTGCACCGTCTGGTTACACCCTGAAAATGGATGATACCAATCATCACTTACATAAACCCGTCATGATTGGTCAG
ATTCGTGCTGATGGTCAATTTGATGTGGTTTACAAAACACCAAATACCATTCGTGCGGAGCCTTGGAGTCCTTACATTCC
AAAATAA

Upstream 100 bases:

>100_bases
ATCTTATTATTAAAAAAATAATATGGCATAAATTTTGAATAATACTAAAAAGTAATTGGTTTTATTGTGTGTAATCAACA
GGATTAGTTGGAGAAAAATC

Downstream 100 bases:

>100_bases
TGAATGAATCTCACCCATTTCAAACGGAATGGGTGATTCCCCATATTTTGATGAAAATATTCAACTGATTAAGGTTCCAA
TATGATGATCAAAGATTATC

Product: branched-chain amino acid ABC transporter periplasmic substrate-binding protein

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MFQQKMMSISLCVAAIMGSAGLVGCSKSSDKPAEQAKAASAPVAQSGDTIKVGILHSLSGTMAISETSLKDTALMTINEI
NAKGGVLGKKLEPVIVDPASDWPLFAEKARQLITQDKVAVIFGCWTSVSRKSVLPVVEELNGLLFYPVQYEGQEQSKNIF
YTGAAPNQQAIPAVEYLMSDEGGKAQRFVLLGTDYVYPRTTNKILRAFLKSKGVADADIMEEYTPFGHNNYQTIVANIKK
FSAGKKTAVISTINGDSNVPFYRELGNQGIKASDIPVMAFSVGEEELRGIDTKPLVGHLAAWNYFMSVKNPKNTDFINKF
KQYAVEYKLPNANKVVTNDPMEATYVGINMWKQAVEKAGSTDVDKVRTAMAGQTFAAPSGYTLKMDDTNHHLHKPVMIGQ
IRADGQFDVVYKTPNTIRAEPWSPYIPK

Sequences:

>Translated_428_residues
MFQQKMMSISLCVAAIMGSAGLVGCSKSSDKPAEQAKAASAPVAQSGDTIKVGILHSLSGTMAISETSLKDTALMTINEI
NAKGGVLGKKLEPVIVDPASDWPLFAEKARQLITQDKVAVIFGCWTSVSRKSVLPVVEELNGLLFYPVQYEGQEQSKNIF
YTGAAPNQQAIPAVEYLMSDEGGKAQRFVLLGTDYVYPRTTNKILRAFLKSKGVADADIMEEYTPFGHNNYQTIVANIKK
FSAGKKTAVISTINGDSNVPFYRELGNQGIKASDIPVMAFSVGEEELRGIDTKPLVGHLAAWNYFMSVKNPKNTDFINKF
KQYAVEYKLPNANKVVTNDPMEATYVGINMWKQAVEKAGSTDVDKVRTAMAGQTFAAPSGYTLKMDDTNHHLHKPVMIGQ
IRADGQFDVVYKTPNTIRAEPWSPYIPK
>Mature_428_residues
MFQQKMMSISLCVAAIMGSAGLVGCSKSSDKPAEQAKAASAPVAQSGDTIKVGILHSLSGTMAISETSLKDTALMTINEI
NAKGGVLGKKLEPVIVDPASDWPLFAEKARQLITQDKVAVIFGCWTSVSRKSVLPVVEELNGLLFYPVQYEGQEQSKNIF
YTGAAPNQQAIPAVEYLMSDEGGKAQRFVLLGTDYVYPRTTNKILRAFLKSKGVADADIMEEYTPFGHNNYQTIVANIKK
FSAGKKTAVISTINGDSNVPFYRELGNQGIKASDIPVMAFSVGEEELRGIDTKPLVGHLAAWNYFMSVKNPKNTDFINKF
KQYAVEYKLPNANKVVTNDPMEATYVGINMWKQAVEKAGSTDVDKVRTAMAGQTFAAPSGYTLKMDDTNHHLHKPVMIGQ
IRADGQFDVVYKTPNTIRAEPWSPYIPK

Specific function: Negatively regulates the expression of the aliphatic amidase operon. AmiC functions by inhibiting the action of AmiR at the protein level. It exhibits protein kinase activity [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000709 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 46786; Mature: 46786

Theoretical pI: Translated: 8.86; Mature: 8.86

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFQQKMMSISLCVAAIMGSAGLVGCSKSSDKPAEQAKAASAPVAQSGDTIKVGILHSLSG
CCHHHHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHCCCCCCCCCCEEEEEEEECCCC
TMAISETSLKDTALMTINEINAKGGVLGKKLEPVIVDPASDWPLFAEKARQLITQDKVAV
CEEEECCCCCCEEEEEEEECCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHCCCEEE
IFGCWTSVSRKSVLPVVEELNGLLFYPVQYEGQEQSKNIFYTGAAPNQQAIPAVEYLMSD
EEEECHHCCHHHHHHHHHHCCCEEEEEEEECCCCCCCCEEEECCCCCCCCCHHHHHHHCC
EGGKAQRFVLLGTDYVYPRTTNKILRAFLKSKGVADADIMEEYTPFGHNNYQTIVANIKK
CCCCEEEEEEEECCEECCCCHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCHHHHHHHHHH
FSAGKKTAVISTINGDSNVPFYRELGNQGIKASDIPVMAFSVGEEELRGIDTKPLVGHLA
HCCCCCEEEEEEECCCCCCCHHHHHCCCCCCCCCCCEEEEECCHHHHHCCCCCCHHHHHH
AWNYFMSVKNPKNTDFINKFKQYAVEYKLPNANKVVTNDPMEATYVGINMWKQAVEKAGS
HHHEEEECCCCCCCHHHHHHHHHEEEEECCCCCEEEECCCCCEEEECHHHHHHHHHHCCC
TDVDKVRTAMAGQTFAAPSGYTLKMDDTNHHLHKPVMIGQIRADGQFDVVYKTPNTIRAE
CCHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCEEEEEEECCCEEEEEEECCCCEECC
PWSPYIPK
CCCCCCCC
>Mature Secondary Structure
MFQQKMMSISLCVAAIMGSAGLVGCSKSSDKPAEQAKAASAPVAQSGDTIKVGILHSLSG
CCHHHHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHCCCCCCCCCCEEEEEEEECCCC
TMAISETSLKDTALMTINEINAKGGVLGKKLEPVIVDPASDWPLFAEKARQLITQDKVAV
CEEEECCCCCCEEEEEEEECCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHCCCEEE
IFGCWTSVSRKSVLPVVEELNGLLFYPVQYEGQEQSKNIFYTGAAPNQQAIPAVEYLMSD
EEEECHHCCHHHHHHHHHHCCCEEEEEEEECCCCCCCCEEEECCCCCCCCCHHHHHHHCC
EGGKAQRFVLLGTDYVYPRTTNKILRAFLKSKGVADADIMEEYTPFGHNNYQTIVANIKK
CCCCEEEEEEEECCEECCCCHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCHHHHHHHHHH
FSAGKKTAVISTINGDSNVPFYRELGNQGIKASDIPVMAFSVGEEELRGIDTKPLVGHLA
HCCCCCEEEEEEECCCCCCCHHHHHCCCCCCCCCCCEEEEECCHHHHHCCCCCCHHHHHH
AWNYFMSVKNPKNTDFINKFKQYAVEYKLPNANKVVTNDPMEATYVGINMWKQAVEKAGS
HHHEEEECCCCCCCHHHHHHHHHEEEEECCCCCEEEECCCCCEEEECHHHHHHHHHHCCC
TDVDKVRTAMAGQTFAAPSGYTLKMDDTNHHLHKPVMIGQIRADGQFDVVYKTPNTIRAE
CCHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCEEEEEEECCCEEEEEEECCCCEECC
PWSPYIPK
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1907262; 10984043; 1762155; 7813419; 10508151; 10708652 [H]