BacMap

Definition	Acinetobacter sp. ADP1 chromosome, complete genome.
Accession	NC_005966
Length	3,598,621

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The map label for this gene is ephD [H]

Identifier: 50084885

GI number: 50084885

Start: 1740301

End: 1741128

Strand: Direct

Name: ephD [H]

Synonym: ACIAD1731

Alternate gene names: 50084885

Gene position: 1740301-1741128 (Clockwise)

Preceding gene: 50084884

Following gene: 50084886

Centisome position: 48.36

GC content: 40.82

Gene sequence:

>828_bases
ATGAAACTATTCAGCTCTCAACATAAAAAGCCAAGTCAACAGGCCTATGCTGTAGTGACTGGTGCAGGTAGTGGCATTGG
GCGTAGTTTTGCACTCGAATTGGCACGTCGCGGCGGTACAGTAGTGTGTTCGGATATCAATCTAGATGCAGCCAAAGAAA
CGGTGAGTTTAATTCAAGAATTGGGTGCACAAGCTTTTCCAGTGCAATGTGATGTGGCTAAAGCTGAACAGGTGTCGCAA
CTGGCAGAGCATGCAGAAAAACTGATGCAAAATCCAGTCACATTGGTGATCAATAATGCAGGTATTGGTTTGGGGGGTAA
GTTTGATGAAATGACCACTGAAGACTGGCAGTGGTGTCTGGATATTAATCTGTGGGGTGTTATTCATGGTTGTCGTGCAT
TTGTTCCCAAATTTAAACAATTAGGACATGGTGCGATTATCAATGTTGCATCAGCGGCTTCATTTACCGCAGCACCTGAA
ATGACGGTATATAACGTCAGCAAAGCAGGGGTCCGTGCATTATCTGAGACCTTATCAGCCGAGCTAAAAAAGTTTGATAT
TAAGGTCAATGTGTTATGCCCGACATTGGTGCCTACCAATATTATCAAAAATGGCAAAGTACCTCATAAAGGAATTTTAG
ATTATGCATTAAACAATTTAGCTTTTACCACCAGTGATAAAGTAGCAAAACTCACATTGGATCGTCTGGATAAAGATAAG
CTTTATACCATTCCACAAATTGATGCCAAATTATTCTGGTTAATGAAGCGAGCAGCACCAAACTTATATGCAAAATTTTT
AGGTACTTCATATCGATTGATCAAATGA

Upstream 100 bases:

>100_bases
CTTATCTGGTAAAAACATTTACGCCACCAGTACGTCACTAATTTTTTGTCGTTTCAGCAATGAAGTTCAGTCAATTGGCT
GAAAACAATAAGGATATGCT

Downstream 100 bases:

>100_bases
GCCCAAAATTTTGGTGACTTATGATCAAATTGAGATGAACACTCAATCAAAGATTGGTTTAAAAATATTTTTAAGTAAAA
GGATATTCTGAATGACAGCT

Product: short-chain dehydrogenase

Products: 3alpha12alpha-dihydroxy-oxobeta-cholanate; NADH [C]

Alternate protein names: NA

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MKLFSSQHKKPSQQAYAVVTGAGSGIGRSFALELARRGGTVVCSDINLDAAKETVSLIQELGAQAFPVQCDVAKAEQVSQ
LAEHAEKLMQNPVTLVINNAGIGLGGKFDEMTTEDWQWCLDINLWGVIHGCRAFVPKFKQLGHGAIINVASAASFTAAPE
MTVYNVSKAGVRALSETLSAELKKFDIKVNVLCPTLVPTNIIKNGKVPHKGILDYALNNLAFTTSDKVAKLTLDRLDKDK
LYTIPQIDAKLFWLMKRAAPNLYAKFLGTSYRLIK

Sequences:

>Translated_275_residues
MKLFSSQHKKPSQQAYAVVTGAGSGIGRSFALELARRGGTVVCSDINLDAAKETVSLIQELGAQAFPVQCDVAKAEQVSQ
LAEHAEKLMQNPVTLVINNAGIGLGGKFDEMTTEDWQWCLDINLWGVIHGCRAFVPKFKQLGHGAIINVASAASFTAAPE
MTVYNVSKAGVRALSETLSAELKKFDIKVNVLCPTLVPTNIIKNGKVPHKGILDYALNNLAFTTSDKVAKLTLDRLDKDK
LYTIPQIDAKLFWLMKRAAPNLYAKFLGTSYRLIK
>Mature_275_residues
MKLFSSQHKKPSQQAYAVVTGAGSGIGRSFALELARRGGTVVCSDINLDAAKETVSLIQELGAQAFPVQCDVAKAEQVSQ
LAEHAEKLMQNPVTLVINNAGIGLGGKFDEMTTEDWQWCLDINLWGVIHGCRAFVPKFKQLGHGAIINVASAASFTAAPE
MTVYNVSKAGVRALSETLSAELKKFDIKVNVLCPTLVPTNIIKNGKVPHKGILDYALNNLAFTTSDKVAKLTLDRLDKDK
LYTIPQIDAKLFWLMKRAAPNLYAKFLGTSYRLIK

Specific function: 7-Alpha-Dehydroxylation Of Cholic Acid, Yielding Deoxycholic Acid And Lithocholic Acid, Respectively. Highest Affinity With Taurochenodeoxycholic Acid. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI25282469, Length=230, Percent_Identity=30.8695652173913, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI15277342, Length=215, Percent_Identity=33.0232558139535, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI40807363, Length=191, Percent_Identity=30.8900523560209, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI32483357, Length=194, Percent_Identity=32.4742268041237, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI210032110, Length=207, Percent_Identity=29.951690821256, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI40254992, Length=191, Percent_Identity=33.5078534031414, Blast_Score=95, Evalue=8e-20,
Organism=Homo sapiens, GI142976729, Length=209, Percent_Identity=31.5789473684211, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI310128830, Length=267, Percent_Identity=25.4681647940075, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI310115468, Length=267, Percent_Identity=25.4681647940075, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI126723191, Length=195, Percent_Identity=31.2820512820513, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI209862901, Length=230, Percent_Identity=28.2608695652174, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI19923983, Length=230, Percent_Identity=28.2608695652174, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI4504505, Length=203, Percent_Identity=29.5566502463054, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI4503817, Length=188, Percent_Identity=30.3191489361702, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI31542939, Length=199, Percent_Identity=30.1507537688442, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI126723750, Length=199, Percent_Identity=30.6532663316583, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI44680136, Length=189, Percent_Identity=27.5132275132275, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI44680133, Length=189, Percent_Identity=27.5132275132275, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI17738292, Length=189, Percent_Identity=27.5132275132275, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI5031737, Length=188, Percent_Identity=31.9148936170213, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI33667109, Length=197, Percent_Identity=30.9644670050761, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI66933014, Length=214, Percent_Identity=28.0373831775701, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI4557649, Length=228, Percent_Identity=27.1929824561404, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI19923817, Length=192, Percent_Identity=25.5208333333333, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI4504503, Length=240, Percent_Identity=27.0833333333333, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI150247226, Length=191, Percent_Identity=29.3193717277487, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI22507405, Length=236, Percent_Identity=27.1186440677966, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI50726952, Length=193, Percent_Identity=29.0155440414508, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI7705925, Length=249, Percent_Identity=29.3172690763052, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI7657478, Length=188, Percent_Identity=28.1914893617021, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI20149619, Length=202, Percent_Identity=27.2277227722772, Blast_Score=67, Evalue=1e-11,
Organism=Escherichia coli, GI1787905, Length=194, Percent_Identity=35.5670103092784, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI2367175, Length=206, Percent_Identity=35.4368932038835, Blast_Score=91, Evalue=6e-20,
Organism=Escherichia coli, GI1790717, Length=186, Percent_Identity=27.4193548387097, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI87082100, Length=187, Percent_Identity=31.0160427807487, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1789208, Length=185, Percent_Identity=30.2702702702703, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1786812, Length=188, Percent_Identity=28.1914893617021, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1787335, Length=232, Percent_Identity=27.1551724137931, Blast_Score=74, Evalue=8e-15,
Organism=Escherichia coli, GI1787820, Length=181, Percent_Identity=28.7292817679558, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1786701, Length=192, Percent_Identity=26.5625, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI87082160, Length=207, Percent_Identity=27.0531400966184, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1788459, Length=188, Percent_Identity=27.6595744680851, Blast_Score=66, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17567345, Length=243, Percent_Identity=30.4526748971193, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17564282, Length=196, Percent_Identity=27.0408163265306, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI25147288, Length=186, Percent_Identity=32.7956989247312, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI193203117, Length=262, Percent_Identity=25.1908396946565, Blast_Score=85, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI193203115, Length=271, Percent_Identity=24.7232472324723, Blast_Score=85, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17568967, Length=209, Percent_Identity=30.1435406698565, Blast_Score=81, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17508895, Length=201, Percent_Identity=24.3781094527363, Blast_Score=75, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI115534660, Length=192, Percent_Identity=31.25, Blast_Score=75, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI32563809, Length=199, Percent_Identity=32.1608040201005, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17507613, Length=199, Percent_Identity=32.1608040201005, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17562910, Length=200, Percent_Identity=30, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17555706, Length=184, Percent_Identity=29.3478260869565, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI115534694, Length=251, Percent_Identity=27.4900398406374, Blast_Score=71, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI193210355, Length=190, Percent_Identity=27.8947368421053, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71994604, Length=194, Percent_Identity=28.3505154639175, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17562904, Length=201, Percent_Identity=26.3681592039801, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17559104, Length=195, Percent_Identity=27.6923076923077, Blast_Score=65, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI71994600, Length=192, Percent_Identity=27.0833333333333, Blast_Score=65, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17557780, Length=200, Percent_Identity=25, Blast_Score=64, Evalue=1e-10,
Organism=Saccharomyces cerevisiae, GI6322861, Length=207, Percent_Identity=32.8502415458937, Blast_Score=86, Evalue=8e-18,
Organism=Saccharomyces cerevisiae, GI6323882, Length=242, Percent_Identity=29.7520661157025, Blast_Score=86, Evalue=9e-18,
Organism=Saccharomyces cerevisiae, GI6320089, Length=206, Percent_Identity=28.6407766990291, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6322067, Length=232, Percent_Identity=27.5862068965517, Blast_Score=74, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24639444, Length=190, Percent_Identity=36.8421052631579, Blast_Score=101, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24649181, Length=191, Percent_Identity=33.5078534031414, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI281364892, Length=202, Percent_Identity=30.1980198019802, Blast_Score=95, Evalue=4e-20,
Organism=Drosophila melanogaster, GI21355631, Length=246, Percent_Identity=27.6422764227642, Blast_Score=90, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24640492, Length=193, Percent_Identity=28.4974093264249, Blast_Score=90, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24584752, Length=200, Percent_Identity=33.5, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI28571526, Length=194, Percent_Identity=31.4432989690722, Blast_Score=85, Evalue=4e-17,
Organism=Drosophila melanogaster, GI23397609, Length=212, Percent_Identity=33.9622641509434, Blast_Score=81, Evalue=7e-16,
Organism=Drosophila melanogaster, GI24643142, Length=189, Percent_Identity=30.1587301587302, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24584748, Length=189, Percent_Identity=28.5714285714286, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI161076933, Length=189, Percent_Identity=28.5714285714286, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI21356301, Length=193, Percent_Identity=29.0155440414508, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24584744, Length=194, Percent_Identity=30.9278350515464, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI28574402, Length=193, Percent_Identity=30.0518134715026, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24640442, Length=211, Percent_Identity=30.3317535545024, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI18859837, Length=204, Percent_Identity=30.3921568627451, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI21357041, Length=204, Percent_Identity=26.9607843137255, Blast_Score=74, Evalue=7e-14,
Organism=Drosophila melanogaster, GI116007236, Length=198, Percent_Identity=31.3131313131313, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24644339, Length=191, Percent_Identity=27.2251308900524, Blast_Score=71, Evalue=9e-13,
Organism=Drosophila melanogaster, GI116007238, Length=201, Percent_Identity=29.8507462686567, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI281364494, Length=202, Percent_Identity=31.1881188118812, Blast_Score=68, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24581726, Length=191, Percent_Identity=29.8429319371728, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24641232, Length=207, Percent_Identity=31.4009661835749, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00561 Abhydrolase_1; PF00106 adh_short [H]

EC number: 1.1.1.159 [C]

Molecular weight: Translated: 29951; Mature: 29951

Theoretical pI: Translated: 9.54; Mature: 9.54

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLFSSQHKKPSQQAYAVVTGAGSGIGRSFALELARRGGTVVCSDINLDAAKETVSLIQE
CCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHH
LGAQAFPVQCDVAKAEQVSQLAEHAEKLMQNPVTLVINNAGIGLGGKFDEMTTEDWQWCL
HCCCCCCEEECHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCEEEE
DINLWGVIHGCRAFVPKFKQLGHGAIINVASAASFTAAPEMTVYNVSKAGVRALSETLSA
EEHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCEEEEECHHHHHHHHHHHHHH
ELKKFDIKVNVLCPTLVPTNIIKNGKVPHKGILDYALNNLAFTTSDKVAKLTLDRLDKDK
HHHHHEEEEEEEECCCCCHHHHCCCCCCCHHHHHHHHHCCEEECCCCHHHHHHHHCCCCC
LYTIPQIDAKLFWLMKRAAPNLYAKFLGTSYRLIK
EEECCCHHHHHHHHHHHHCHHHHHHHHCCCCEECC
>Mature Secondary Structure
MKLFSSQHKKPSQQAYAVVTGAGSGIGRSFALELARRGGTVVCSDINLDAAKETVSLIQE
CCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHH
LGAQAFPVQCDVAKAEQVSQLAEHAEKLMQNPVTLVINNAGIGLGGKFDEMTTEDWQWCL
HCCCCCCEEECHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCEEEE
DINLWGVIHGCRAFVPKFKQLGHGAIINVASAASFTAAPEMTVYNVSKAGVRALSETLSA
EEHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCEEEEECHHHHHHHHHHHHHH
ELKKFDIKVNVLCPTLVPTNIIKNGKVPHKGILDYALNNLAFTTSDKVAKLTLDRLDKDK
HHHHHEEEEEEEECCCCCHHHHCCCCCCCHHHHHHHHHCCEEECCCCHHHHHHHHCCCCC
LYTIPQIDAKLFWLMKRAAPNLYAKFLGTSYRLIK
EEECCCHHHHHHHHHHHHCHHHHHHHHCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: 2-Mercaptoethanol; Dithiothreitol; NAD+; NADH [C]

Metal ions: CaCl2; KCl; MnCl2; NaCl [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.8 {cholic} 0.085 {glycochenodeoxycholic} 0.24 {taurochenodeoxycholic} 1 {glycocholic} 0.06 {chenodeoxycholic} [C]

Substrates: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate!; NAD [C]

Specific reaction: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate! + NAD+ = 3alpha12alpha-dihydroxy-oxobeta-cholanate + NADH [C]

General reaction: Redox reaction [C]

Inhibitor: Ascorbicacid; BaCl2; CoCl2; CuCl2; EDTA; FeCl3; HgCl2; MgCl2; NH4Cl; Potassiumoxalate; Sodiumcitrate; Sodiumlaurylsulfate; Sodiumperchlorate; Sodiumperiodate; Sodiumpersulfate; TritonX-100; Tween; ZnCl2 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]