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The map label for this gene is pcaF [H]

Identifier: 50084860

GI number: 50084860

Start: 1710591

End: 1711796

Strand: Direct

Name: pcaF [H]

Synonym: ACIAD1706

Alternate gene names: 50084860

Gene position: 1710591-1711796 (Clockwise)

Preceding gene: 50084859

Following gene: 50084861

Centisome position: 47.53

GC content: 56.3

Gene sequence:

>1206_bases
ATGAAACATGCATACATCGTCGATGCGATCCGTACCCCATTTGGCCGCTATGCAGGCGGATTGGCAGCAGTACGTGCGGA
TGATCTCGGCGCAATCCCGATTGCTGCCCTGATCGAACGCAACCCAAGCGTGAACTGGGCACAGGTCGATGACGTGATCT
ACGGCTGTGCCAATCAGGCAGGAGAAGACAACCGTAACGTTGGCCGTATGTCGGCACTGCTGGCAGGTCTACCGGTCGAA
GTGCCGGCAACGACGGTCAACCGCCTGTGTGGCTCATCTCTGGATGCAATCGCCATGGCAGCACGTGCGATCAAAGCAGG
CGAAGCCCATCTGATCATTGCAGGGGGTGTCGAAAGCATGAGCCGCGCACCGTATGTGATGGGGAAATCAGAAGGGGCGT
TTGGACGCACCCAGAAGATAGAAGACACCACCATGGGCTGGCGCTTCATCAATCCGAAGCTTAAAGCCATGTATGGTGTG
GATACCATGCCGCAAACCGCAGAAAATGTGGCCGAGCAGTTTGGCATCCAGCGTGAAGACCAAGATCAGTTTGCCTACAC
CAGCCAGCAACGCACCGCGGCGGCACAGGCCAAAGGCTACTTTGCAAAAGAAATCGTGCCTGTCACGATTCCACAGCGCA
AAGGAGAGCCGGTGGTGATCGACACCGATGAGCATCCACGTGCCTCAACCACACTAGAGGGACTGGCCAAGCTAAAAGGC
GTGGTCAAGCCAGAAGGTAGCGTGACAGCGGGCAATGCGTCAGGCATCAACGACGGTGCAGCGGCAGTTCTGATTGCATC
AGACGAAGCGGTTGCACAATACCAGCTCAAAGCACGTGCCAAGATCATTGCGTCCACCACGGTAGGCATCGAGCCACGCA
TCATGGGCTTTGCACCGGCACCGGCGATTAAGAAGCTACTCAAGCAGGCCAATCTGACGCTTGATCAGATGGATGTGATT
GAGCTGAACGAAGCCTTTGCTGCACAGGCACTGGCCTGTACCCGAGACTTAGGTCTGGCGGATGACGATGCACGGGTGAA
TCCGAATGGGGGTGCGATTGCACTGGGCCATCCATTGGGTGCATCTGGTGCACGTTTAGTGACGACGGCTTTAAACCAGC
TTGAACAGTCGGGCGGTAAGTATGCGCTGTGTTCGATGTGTATCGGCGTGGGTCAGGGGATTGCGCTCATTATTGAGCGG
GTTTGA

Upstream 100 bases:

>100_bases
GAAAGTGATCGAGAAAGTCGAAGGCTTAAGCTTTGAAGAACTACAACGCCTGACCGGTGCAACACTGATCGATGCGACAC
AAGGCTAAGGATAACCGAAT

Downstream 100 bases:

>100_bases
GGGGCGTCATCATGAGCCAGTTATATGCCAGCTTGTTTTACCAAAGAGATGTGACTGAAATCTTCAGTGATCGCGCTTTA
GTGTCATATATGGTTGAAGC

Product: beta-ketoadipyl CoA thiolase

Products: NA

Alternate protein names: 3-oxoadipyl-CoA thiolase [H]

Number of amino acids: Translated: 401; Mature: 401

Protein sequence:

>401_residues
MKHAYIVDAIRTPFGRYAGGLAAVRADDLGAIPIAALIERNPSVNWAQVDDVIYGCANQAGEDNRNVGRMSALLAGLPVE
VPATTVNRLCGSSLDAIAMAARAIKAGEAHLIIAGGVESMSRAPYVMGKSEGAFGRTQKIEDTTMGWRFINPKLKAMYGV
DTMPQTAENVAEQFGIQREDQDQFAYTSQQRTAAAQAKGYFAKEIVPVTIPQRKGEPVVIDTDEHPRASTTLEGLAKLKG
VVKPEGSVTAGNASGINDGAAAVLIASDEAVAQYQLKARAKIIASTTVGIEPRIMGFAPAPAIKKLLKQANLTLDQMDVI
ELNEAFAAQALACTRDLGLADDDARVNPNGGAIALGHPLGASGARLVTTALNQLEQSGGKYALCSMCIGVGQGIALIIER
V

Sequences:

>Translated_401_residues
MKHAYIVDAIRTPFGRYAGGLAAVRADDLGAIPIAALIERNPSVNWAQVDDVIYGCANQAGEDNRNVGRMSALLAGLPVE
VPATTVNRLCGSSLDAIAMAARAIKAGEAHLIIAGGVESMSRAPYVMGKSEGAFGRTQKIEDTTMGWRFINPKLKAMYGV
DTMPQTAENVAEQFGIQREDQDQFAYTSQQRTAAAQAKGYFAKEIVPVTIPQRKGEPVVIDTDEHPRASTTLEGLAKLKG
VVKPEGSVTAGNASGINDGAAAVLIASDEAVAQYQLKARAKIIASTTVGIEPRIMGFAPAPAIKKLLKQANLTLDQMDVI
ELNEAFAAQALACTRDLGLADDDARVNPNGGAIALGHPLGASGARLVTTALNQLEQSGGKYALCSMCIGVGQGIALIIER
V
>Mature_401_residues
MKHAYIVDAIRTPFGRYAGGLAAVRADDLGAIPIAALIERNPSVNWAQVDDVIYGCANQAGEDNRNVGRMSALLAGLPVE
VPATTVNRLCGSSLDAIAMAARAIKAGEAHLIIAGGVESMSRAPYVMGKSEGAFGRTQKIEDTTMGWRFINPKLKAMYGV
DTMPQTAENVAEQFGIQREDQDQFAYTSQQRTAAAQAKGYFAKEIVPVTIPQRKGEPVVIDTDEHPRASTTLEGLAKLKG
VVKPEGSVTAGNASGINDGAAAVLIASDEAVAQYQLKARAKIIASTTVGIEPRIMGFAPAPAIKKLLKQANLTLDQMDVI
ELNEAFAAQALACTRDLGLADDDARVNPNGGAIALGHPLGASGARLVTTALNQLEQSGGKYALCSMCIGVGQGIALIIER
V

Specific function: Catalyzes thiolytic cleavage of beta-ketoadipyl-CoA to succinyl-CoA and acetyl-CoA [H]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI167614485, Length=401, Percent_Identity=43.3915211970075, Blast_Score=299, Evalue=4e-81,
Organism=Homo sapiens, GI148539872, Length=398, Percent_Identity=41.7085427135678, Blast_Score=279, Evalue=4e-75,
Organism=Homo sapiens, GI4501853, Length=400, Percent_Identity=41.25, Blast_Score=242, Evalue=4e-64,
Organism=Homo sapiens, GI4557237, Length=402, Percent_Identity=36.318407960199, Blast_Score=232, Evalue=4e-61,
Organism=Homo sapiens, GI4504327, Length=431, Percent_Identity=31.7865429234339, Blast_Score=183, Evalue=3e-46,
Organism=Homo sapiens, GI194353979, Length=400, Percent_Identity=32, Blast_Score=152, Evalue=5e-37,
Organism=Escherichia coli, GI1787663, Length=401, Percent_Identity=61.3466334164589, Blast_Score=508, Evalue=1e-145,
Organism=Escherichia coli, GI48994986, Length=404, Percent_Identity=44.8019801980198, Blast_Score=305, Evalue=5e-84,
Organism=Escherichia coli, GI87082165, Length=402, Percent_Identity=44.7761194029851, Blast_Score=301, Evalue=4e-83,
Organism=Escherichia coli, GI1788554, Length=402, Percent_Identity=44.7761194029851, Blast_Score=279, Evalue=2e-76,
Organism=Escherichia coli, GI1788683, Length=423, Percent_Identity=31.2056737588652, Blast_Score=164, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI133906874, Length=402, Percent_Identity=44.2786069651741, Blast_Score=307, Evalue=8e-84,
Organism=Caenorhabditis elegans, GI25147385, Length=402, Percent_Identity=35.8208955223881, Blast_Score=234, Evalue=5e-62,
Organism=Caenorhabditis elegans, GI17535921, Length=401, Percent_Identity=33.9152119700748, Blast_Score=202, Evalue=2e-52,
Organism=Caenorhabditis elegans, GI17551802, Length=429, Percent_Identity=34.2657342657343, Blast_Score=195, Evalue=4e-50,
Organism=Caenorhabditis elegans, GI17535917, Length=405, Percent_Identity=31.358024691358, Blast_Score=158, Evalue=4e-39,
Organism=Saccharomyces cerevisiae, GI6325229, Length=410, Percent_Identity=37.8048780487805, Blast_Score=238, Evalue=1e-63,
Organism=Saccharomyces cerevisiae, GI6322031, Length=407, Percent_Identity=36.8550368550369, Blast_Score=212, Evalue=7e-56,
Organism=Drosophila melanogaster, GI24655093, Length=402, Percent_Identity=47.5124378109453, Blast_Score=339, Evalue=2e-93,
Organism=Drosophila melanogaster, GI17648125, Length=406, Percent_Identity=42.8571428571429, Blast_Score=288, Evalue=5e-78,
Organism=Drosophila melanogaster, GI24640423, Length=397, Percent_Identity=37.7833753148615, Blast_Score=222, Evalue=4e-58,
Organism=Drosophila melanogaster, GI17137578, Length=426, Percent_Identity=33.5680751173709, Blast_Score=191, Evalue=7e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012793
- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.174 [H]

Molecular weight: Translated: 42096; Mature: 42096

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHAYIVDAIRTPFGRYAGGLAAVRADDLGAIPIAALIERNPSVNWAQVDDVIYGCANQA
CCCEEEHHHHHCCHHHHHCCEEEEECCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHCCC
GEDNRNVGRMSALLAGLPVEVPATTVNRLCGSSLDAIAMAARAIKAGEAHLIIAGGVESM
CCCCCCHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEECCHHHH
SRAPYVMGKSEGAFGRTQKIEDTTMGWRFINPKLKAMYGVDTMPQTAENVAEQFGIQRED
CCCCEEEECCCCCCCCCCCCCCCCCCEEEECCHHHHEECCCCCCHHHHHHHHHHCCCCCC
QDQFAYTSQQRTAAAQAKGYFAKEIVPVTIPQRKGEPVVIDTDEHPRASTTLEGLAKLKG
CHHHHHHHHHHHHHHHHCCCHHHCEEEEECCCCCCCEEEEECCCCCCHHHHHHHHHHHCC
VVKPEGSVTAGNASGINDGAAAVLIASDEAVAQYQLKARAKIIASTTVGIEPRIMGFAPA
CCCCCCCEECCCCCCCCCCCEEEEEECCHHHHHHHHHHHHEEEEEEECCCCCEEEECCCC
PAIKKLLKQANLTLDQMDVIELNEAFAAQALACTRDLGLADDDARVNPNGGAIALGHPLG
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCEEEECCCCC
ASGARLVTTALNQLEQSGGKYALCSMCIGVGQGIALIIERV
CCCHHHHHHHHHHHHHCCCCEEHHHHHHHCCCCCEEEEECC
>Mature Secondary Structure
MKHAYIVDAIRTPFGRYAGGLAAVRADDLGAIPIAALIERNPSVNWAQVDDVIYGCANQA
CCCEEEHHHHHCCHHHHHCCEEEEECCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHCCC
GEDNRNVGRMSALLAGLPVEVPATTVNRLCGSSLDAIAMAARAIKAGEAHLIIAGGVESM
CCCCCCHHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEECCHHHH
SRAPYVMGKSEGAFGRTQKIEDTTMGWRFINPKLKAMYGVDTMPQTAENVAEQFGIQRED
CCCCEEEECCCCCCCCCCCCCCCCCCEEEECCHHHHEECCCCCCHHHHHHHHHHCCCCCC
QDQFAYTSQQRTAAAQAKGYFAKEIVPVTIPQRKGEPVVIDTDEHPRASTTLEGLAKLKG
CHHHHHHHHHHHHHHHHCCCHHHCEEEEECCCCCCCEEEEECCCCCCHHHHHHHHHHHCC
VVKPEGSVTAGNASGINDGAAAVLIASDEAVAQYQLKARAKIIASTTVGIEPRIMGFAPA
CCCCCCCEECCCCCCCCCCCEEEEEECCHHHHHHHHHHHHEEEEEEECCCCCEEEECCCC
PAIKKLLKQANLTLDQMDVIELNEAFAAQALACTRDLGLADDDARVNPNGGAIALGHPLG
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCEEEECCCCC
ASGARLVTTALNQLEQSGGKYALCSMCIGVGQGIALIIERV
CCCHHHHHHHHHHHHHCCCCEEHHHHHHHCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8125318 [H]