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The map label for this gene is trmA

Identifier: 50084803

GI number: 50084803

Start: 1640614

End: 1641699

Strand: Reverse

Name: trmA

Synonym: ACIAD1645

Alternate gene names: 50084803

Gene position: 1641699-1640614 (Counterclockwise)

Preceding gene: 50084804

Following gene: 50084802

Centisome position: 45.62

GC content: 38.03

Gene sequence:

>1086_bases
ATGTCTCAAACCTATCAGCAGCAGTTGCAAAGCAAAATTGAACGAATCACCCATCAATTCGCAGAATTTAACCCACCTGC
CCTTGAAGTTTTTGAATCTCCCGAGCAGCATTTTCGTATGCGTGCAGAATTTCGAATCTGGCATACAGATAATGATTTAT
TTTATGCAATGTTTGAACGCAATGAGGACGGTAAGCAAAAAGAAGTGATTCGTGTAGACGAATTTCCAATTGCAGATCAA
AGTATCAATCAACTCATGCCACGCTTGTTAGAAGAACTCAAAGCCAATCCAACGTTATCTCAGCGAATCTTTGAAGCTGA
TTTTTTAACGACTTTAAGCGGTGAAATGCTAGTTACGCTCATTTACCATCGAAAGTTAGATACAGAATGGGAAAGTGCTG
CTAAAGCTTTGGCCGAAAAATTAAATATTAAAATTATGGGTCGTAGTCGCGGTCAAAAGGTAATTATTGGCGATGACTAC
GTAGTAGAGCAACTCAATGTGCATGGTCGCACTTTTAAATACAAACAAATCGAAAGTAGCTTTACCCAACCCAATGCTGA
GGTTTGCCAAAAGATGCTCACCTGGGCATGTGATGTAATTCAGGGTTCAAATCAGGATTTGCTCGAGTTGTATTGTGGAA
ATGGCAATTTCACCCTGCCATTGTCATTACATTTTCGCCGAGTTCTTGCAACAGAATTAGCCAAAAGTTCAGTTTATGCT
GCGCAATGGAATATCGAGCAAAATCAGATCGAAAATATTCAAATTGCACGTCTTTCTGCAGAAGAATTTACGCAGGCTTA
TCAAGGTGAACGTGAATTCAGACGTTTGCAAGAAGCTAATATTGATATTCAAAGCTATGAGTTTGATACGATATTTGTTG
ATCCGCCACGTGCTGGTATCGACGATGAAACCTTAAAACTCCTTAAAGGCTTTAAACGAATTCTATATATTTCATGCAAT
CCAGATACCTTATACAACAATTTAAAAACATTGAGTCAAACACATAAAATTACTCGTTTTGCTTTGTTTGATCAGTTCCC
TTTTACTCACCATGTTGAGTCTGGTGTGTTACTTGAATTAATTTAA

Upstream 100 bases:

>100_bases
TCAAAAATATTACAAGTGCAATAAGGCCAAGTTTTCTCCCTGTGAATGTGTTTTTTTGCTAGTATTTGCGCTTTGATTGA
TCTTGATATTTTACTTATTT

Downstream 100 bases:

>100_bases
ACAAATTATTGCAATGTAGATAACATGTAATAGGTTACGACTGTAACCTATTATTATTTATGAAAATTTACACTTAAAAA
TACAATATAAATCAAAGATC

Product: tRNA (uracil-5-)-methyltransferase

Products: NA

Alternate protein names: tRNA(M-5-U54)-methyltransferase; RUMT

Number of amino acids: Translated: 361; Mature: 360

Protein sequence:

>361_residues
MSQTYQQQLQSKIERITHQFAEFNPPALEVFESPEQHFRMRAEFRIWHTDNDLFYAMFERNEDGKQKEVIRVDEFPIADQ
SINQLMPRLLEELKANPTLSQRIFEADFLTTLSGEMLVTLIYHRKLDTEWESAAKALAEKLNIKIMGRSRGQKVIIGDDY
VVEQLNVHGRTFKYKQIESSFTQPNAEVCQKMLTWACDVIQGSNQDLLELYCGNGNFTLPLSLHFRRVLATELAKSSVYA
AQWNIEQNQIENIQIARLSAEEFTQAYQGEREFRRLQEANIDIQSYEFDTIFVDPPRAGIDDETLKLLKGFKRILYISCN
PDTLYNNLKTLSQTHKITRFALFDQFPFTHHVESGVLLELI

Sequences:

>Translated_361_residues
MSQTYQQQLQSKIERITHQFAEFNPPALEVFESPEQHFRMRAEFRIWHTDNDLFYAMFERNEDGKQKEVIRVDEFPIADQ
SINQLMPRLLEELKANPTLSQRIFEADFLTTLSGEMLVTLIYHRKLDTEWESAAKALAEKLNIKIMGRSRGQKVIIGDDY
VVEQLNVHGRTFKYKQIESSFTQPNAEVCQKMLTWACDVIQGSNQDLLELYCGNGNFTLPLSLHFRRVLATELAKSSVYA
AQWNIEQNQIENIQIARLSAEEFTQAYQGEREFRRLQEANIDIQSYEFDTIFVDPPRAGIDDETLKLLKGFKRILYISCN
PDTLYNNLKTLSQTHKITRFALFDQFPFTHHVESGVLLELI
>Mature_360_residues
SQTYQQQLQSKIERITHQFAEFNPPALEVFESPEQHFRMRAEFRIWHTDNDLFYAMFERNEDGKQKEVIRVDEFPIADQS
INQLMPRLLEELKANPTLSQRIFEADFLTTLSGEMLVTLIYHRKLDTEWESAAKALAEKLNIKIMGRSRGQKVIIGDDYV
VEQLNVHGRTFKYKQIESSFTQPNAEVCQKMLTWACDVIQGSNQDLLELYCGNGNFTLPLSLHFRRVLATELAKSSVYAA
QWNIEQNQIENIQIARLSAEEFTQAYQGEREFRRLQEANIDIQSYEFDTIFVDPPRAGIDDETLKLLKGFKRILYISCNP
DTLYNNLKTLSQTHKITRFALFDQFPFTHHVESGVLLELI

Specific function: Catalyzes the formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA M5U methyltransferase family. TrmA subfamily

Homologues:

Organism=Escherichia coli, GI1790403, Length=362, Percent_Identity=53.8674033149171, Blast_Score=406, Evalue=1e-114,
Organism=Escherichia coli, GI1789148, Length=187, Percent_Identity=32.620320855615, Blast_Score=90, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24666579, Length=230, Percent_Identity=25.2173913043478, Blast_Score=79, Evalue=7e-15,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRMA_ACIAD (Q6FBS0)

Other databases:

- EMBL:   CR543861
- RefSeq:   YP_046313.1
- ProteinModelPortal:   Q6FBS0
- SMR:   Q6FBS0
- STRING:   Q6FBS0
- GeneID:   2880728
- GenomeReviews:   CR543861_GR
- KEGG:   aci:ACIAD1645
- NMPDR:   fig|62977.3.peg.1459
- eggNOG:   COG2265
- HOGENOM:   HBG608402
- OMA:   NEVRPFR
- PhylomeDB:   Q6FBS0
- ProtClustDB:   PRK05031
- BioCyc:   ASP62977:ACIAD1645-MONOMER
- HAMAP:   MF_01011
- InterPro:   IPR011869
- InterPro:   IPR010280
- TIGRFAMs:   TIGR02143

Pfam domain/function: PF05958 tRNA_U5-meth_tr

EC number: =2.1.1.35

Molecular weight: Translated: 42118; Mature: 41987

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS01230 TRMA_1; PS01231 TRMA_2

Important sites: ACT_SITE 319-319 BINDING 183-183 BINDING 211-211 BINDING 216-216 BINDING 232-232 BINDING 294-294

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQTYQQQLQSKIERITHQFAEFNPPALEVFESPEQHFRMRAEFRIWHTDNDLFYAMFER
CCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCHHHHEEEEEEEEEECCCCEEEEEEEC
NEDGKQKEVIRVDEFPIADQSINQLMPRLLEELKANPTLSQRIFEADFLTTLSGEMLVTL
CCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHH
IYHRKLDTEWESAAKALAEKLNIKIMGRSRGQKVIIGDDYVVEQLNVHGRTFKYKQIESS
HHHHHCCCCHHHHHHHHHHHCCEEEEECCCCCEEEECCCHHHHHHCCCCCEEEHHHHHHH
FTQPNAEVCQKMLTWACDVIQGSNQDLLELYCGNGNFTLPLSLHFRRVLATELAKSSVYA
CCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEHHHHHHHHHHHHHHHHCEEE
AQWNIEQNQIENIQIARLSAEEFTQAYQGEREFRRLQEANIDIQSYEFDTIFVDPPRAGI
EEECCCHHHHCCEEEEEECHHHHHHHHCCHHHHHHHHHCCCCEEEEEEEEEEECCCCCCC
DDETLKLLKGFKRILYISCNPDTLYNNLKTLSQTHKITRFALFDQFPFTHHVESGVLLEL
CHHHHHHHHHHHEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCEEEEE
I
C
>Mature Secondary Structure 
SQTYQQQLQSKIERITHQFAEFNPPALEVFESPEQHFRMRAEFRIWHTDNDLFYAMFER
CHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCHHHHEEEEEEEEEECCCCEEEEEEEC
NEDGKQKEVIRVDEFPIADQSINQLMPRLLEELKANPTLSQRIFEADFLTTLSGEMLVTL
CCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHH
IYHRKLDTEWESAAKALAEKLNIKIMGRSRGQKVIIGDDYVVEQLNVHGRTFKYKQIESS
HHHHHCCCCHHHHHHHHHHHCCEEEEECCCCCEEEECCCHHHHHHCCCCCEEEHHHHHHH
FTQPNAEVCQKMLTWACDVIQGSNQDLLELYCGNGNFTLPLSLHFRRVLATELAKSSVYA
CCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEHHHHHHHHHHHHHHHHCEEE
AQWNIEQNQIENIQIARLSAEEFTQAYQGEREFRRLQEANIDIQSYEFDTIFVDPPRAGI
EEECCCHHHHCCEEEEEECHHHHHHHHCCHHHHHHHHHCCCCEEEEEEEEEEECCCCCCC
DDETLKLLKGFKRILYISCNPDTLYNNLKTLSQTHKITRFALFDQFPFTHHVESGVLLEL
CHHHHHHHHHHHEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCEEEEE
I
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA