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The map label for this gene is nifA [H]

Identifier: 50084782

GI number: 50084782

Start: 1620783

End: 1621742

Strand: Reverse

Name: nifA [H]

Synonym: ACIAD1624

Alternate gene names: 50084782

Gene position: 1621742-1620783 (Counterclockwise)

Preceding gene: 50084792

Following gene: 50084780

Centisome position: 45.07

GC content: 37.92

Gene sequence:

>960_bases
ATGAAACAGCATATCGAACTTCAATCAAGGTTCCATAGTGAATCTGCTAAAATGCAGCATATGTTTTCAATTATAAAAAA
AGTTGCCATAACTGAGTTTCCAGTATTGGTTCGAGGAGAATCTGGCAGTGGCAAAGAACTTGTGGCGCGTGCCATTCATG
ATTTGAGTCACCGTAAAAATGAGGTATTTATTGCGGTCAATTGTGCGGCTTTAAATGAGAATATTCTTGAAAGTGAGCTT
TTTGGACATGTTAAAGGTGCATTTACAGGTGCAATCCGTGAGCATAAAGGTGTGTTTGAACGCGCCAAAGGCGGTACACT
TTTTCTAGATGAAATTGCAGAAATTCCAATGGCTTTACAAGCCAAATTATTACGTGTTTTGGAAGCTGGAGAATTTACCC
CATTGGGTGGAGAATATGCGATCAAATCTAATGTACGTATTGTGACTGCAACGCATCGTGCCTTAAGAGAAGAAGCAAAA
GTTGGAAATTTTCGTCAAGATTTACTCTATCGTTTACGCGTGATTCCAATTTTTGTTCCACCTCTACGTGAGCGTAAAGA
AGATATTGCTCATATCGCTCAGTATATTCTTGATCAACAACACCATGATTCGACCTCTAAACCCGCGTTGACTCAGGCTG
CACTTGAAGTTTTGCTTAATTACGACTGGCCAGGCAATGTACGTGAACTTAAGAACACTTTACTCTATGCACTCACCATG
ACAGATCATCCCTCAGCAATTGATGTTCATGACTTACCTGATGAAATATATAATTTAGAAGATTATTCTGTGCAATCATC
AAAATCTATTAGCGAACACTTTCCAAAGTATGGAAAATTTACTTATCACTTGATTCAAGAAACACTCATAAAATATCACT
ATGATTTAGCTTTAACCGCTAAAGCATTAGGAATCGGGCGTACGACCTTATGGCGATATAGAAAACAATATAATTTATAG

Upstream 100 bases:

>100_bases
TAAAATCAAATATTTATATCAATTCATTGAAATTTATAATTGCTAATTGTTTAATTTGTTCCATAATTTAAAACTAAATA
TTCTATTTTTGAAACATATA

Downstream 100 bases:

>100_bases
TCTTTTCACATCAAGTATTCAACTTAACTTGGATGTTGATACAACATGAATATGCTGGATCGTCTGATTACAGATTGGTG
AACAGATATTGGAGATTTAT

Product: transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MKQHIELQSRFHSESAKMQHMFSIIKKVAITEFPVLVRGESGSGKELVARAIHDLSHRKNEVFIAVNCAALNENILESEL
FGHVKGAFTGAIREHKGVFERAKGGTLFLDEIAEIPMALQAKLLRVLEAGEFTPLGGEYAIKSNVRIVTATHRALREEAK
VGNFRQDLLYRLRVIPIFVPPLRERKEDIAHIAQYILDQQHHDSTSKPALTQAALEVLLNYDWPGNVRELKNTLLYALTM
TDHPSAIDVHDLPDEIYNLEDYSVQSSKSISEHFPKYGKFTYHLIQETLIKYHYDLALTAKALGIGRTTLWRYRKQYNL

Sequences:

>Translated_319_residues
MKQHIELQSRFHSESAKMQHMFSIIKKVAITEFPVLVRGESGSGKELVARAIHDLSHRKNEVFIAVNCAALNENILESEL
FGHVKGAFTGAIREHKGVFERAKGGTLFLDEIAEIPMALQAKLLRVLEAGEFTPLGGEYAIKSNVRIVTATHRALREEAK
VGNFRQDLLYRLRVIPIFVPPLRERKEDIAHIAQYILDQQHHDSTSKPALTQAALEVLLNYDWPGNVRELKNTLLYALTM
TDHPSAIDVHDLPDEIYNLEDYSVQSSKSISEHFPKYGKFTYHLIQETLIKYHYDLALTAKALGIGRTTLWRYRKQYNL
>Mature_319_residues
MKQHIELQSRFHSESAKMQHMFSIIKKVAITEFPVLVRGESGSGKELVARAIHDLSHRKNEVFIAVNCAALNENILESEL
FGHVKGAFTGAIREHKGVFERAKGGTLFLDEIAEIPMALQAKLLRVLEAGEFTPLGGEYAIKSNVRIVTATHRALREEAK
VGNFRQDLLYRLRVIPIFVPPLRERKEDIAHIAQYILDQQHHDSTSKPALTQAALEVLLNYDWPGNVRELKNTLLYALTM
TDHPSAIDVHDLPDEIYNLEDYSVQSSKSISEHFPKYGKFTYHLIQETLIKYHYDLALTAKALGIGRTTLWRYRKQYNL

Specific function: Required for activation of most nif operons, which are directly involved in nitrogen fixation [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1789233, Length=325, Percent_Identity=41.5384615384615, Blast_Score=234, Evalue=6e-63,
Organism=Escherichia coli, GI1788550, Length=314, Percent_Identity=42.0382165605096, Blast_Score=229, Evalue=2e-61,
Organism=Escherichia coli, GI87082117, Length=229, Percent_Identity=48.0349344978166, Blast_Score=222, Evalue=3e-59,
Organism=Escherichia coli, GI1790437, Length=229, Percent_Identity=48.471615720524, Blast_Score=218, Evalue=4e-58,
Organism=Escherichia coli, GI87081872, Length=320, Percent_Identity=41.25, Blast_Score=216, Evalue=1e-57,
Organism=Escherichia coli, GI87082152, Length=319, Percent_Identity=39.1849529780564, Blast_Score=216, Evalue=2e-57,
Organism=Escherichia coli, GI1790299, Length=249, Percent_Identity=46.1847389558233, Blast_Score=215, Evalue=4e-57,
Organism=Escherichia coli, GI1788905, Length=238, Percent_Identity=45.3781512605042, Blast_Score=211, Evalue=5e-56,
Organism=Escherichia coli, GI1786524, Length=321, Percent_Identity=40.4984423676012, Blast_Score=206, Evalue=2e-54,
Organism=Escherichia coli, GI1789087, Length=242, Percent_Identity=45.4545454545455, Blast_Score=202, Evalue=2e-53,
Organism=Escherichia coli, GI1787583, Length=241, Percent_Identity=39.0041493775934, Blast_Score=176, Evalue=1e-45,
Organism=Escherichia coli, GI1789828, Length=273, Percent_Identity=34.7985347985348, Blast_Score=144, Evalue=8e-36,
Organism=Escherichia coli, GI87081858, Length=304, Percent_Identity=31.5789473684211, Blast_Score=124, Evalue=1e-29,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR010113
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 36384; Mature: 36384

Theoretical pI: Translated: 8.41; Mature: 8.41

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQHIELQSRFHSESAKMQHMFSIIKKVAITEFPVLVRGESGSGKELVARAIHDLSHRKN
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHHHCCCC
EVFIAVNCAALNENILESELFGHVKGAFTGAIREHKGVFERAKGGTLFLDEIAEIPMALQ
EEEEEEEEHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHCCHHHH
AKLLRVLEAGEFTPLGGEYAIKSNVRIVTATHRALREEAKVGNFRQDLLYRLRVIPIFVP
HHHHHHHHCCCCCCCCCCEEEECCCEEEEHHHHHHHHHHHCCCHHHHHHHHHHHHHEECC
PLRERKEDIAHIAQYILDQQHHDSTSKPALTQAALEVLLNYDWPGNVRELKNTLLYALTM
CHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHEEEEEE
TDHPSAIDVHDLPDEIYNLEDYSVQSSKSISEHFPKYGKFTYHLIQETLIKYHYDLALTA
CCCCCCCCCCCCCHHHHCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
KALGIGRTTLWRYRKQYNL
HHHCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MKQHIELQSRFHSESAKMQHMFSIIKKVAITEFPVLVRGESGSGKELVARAIHDLSHRKN
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHHHCCCC
EVFIAVNCAALNENILESELFGHVKGAFTGAIREHKGVFERAKGGTLFLDEIAEIPMALQ
EEEEEEEEHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHCCHHHH
AKLLRVLEAGEFTPLGGEYAIKSNVRIVTATHRALREEAKVGNFRQDLLYRLRVIPIFVP
HHHHHHHHCCCCCCCCCCEEEECCCEEEEHHHHHHHHHHHCCCHHHHHHHHHHHHHEECC
PLRERKEDIAHIAQYILDQQHHDSTSKPALTQAALEVLLNYDWPGNVRELKNTLLYALTM
CHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHEEEEEE
TDHPSAIDVHDLPDEIYNLEDYSVQSSKSISEHFPKYGKFTYHLIQETLIKYHYDLALTA
CCCCCCCCCCCCCHHHHCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
KALGIGRTTLWRYRKQYNL
HHHCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3027503 [H]