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The map label for this gene is nthB [H]

Identifier: 50084776

GI number: 50084776

Start: 1613938

End: 1614582

Strand: Reverse

Name: nthB [H]

Synonym: ACIAD1615

Alternate gene names: 50084776

Gene position: 1614582-1613938 (Counterclockwise)

Preceding gene: 50084777

Following gene: 50084775

Centisome position: 44.87

GC content: 43.26

Gene sequence:

>645_bases
ATGGATGGTTTTCATGATTTAGGTGGTGTACAAGGCTTTGGTGCTATTGATCATAAGATTAATTCTAAAAGCTACAAACC
TGTCTTTAAAAAAGATTGGGAACATCTTGCCTACACTTTAATGTTTTTAGGTGCTGGGCATCTAAATAAATTCAATATTG
ATCAGGTTCGCCATGCCGTTGAACGTATTGGATTACGTCAACATGTTGGAACTGAATATTATGAACGTTATGTGATCGCA
ACAGCTACATTACTGACCGAAGCAGGTGTAATTACCCAGCAAGAACTGGACGAAGCCCTGGGTTCACATTTCCAGCTTGC
GACTCCGAATCATTCTACTGGCAGATCAGCTATTGCAGATCGTCCACCTTTTGAAGTGGGTGACCAAGTGACTGTGCGTG
ATGAATACGTTTCGGGCCACATTCGAGTGCCTGCTTATGTACGAGGTAAAACTGGCGTCGTGGTTCATCGTACGACAGAA
AAATGGCCTTTCCCAGATGCGGCTGGTCATGGACATACTGATGCACTCCCTCAACCTACCTATCATGTGGAATTTAACAC
CCAGGAATTATGGGGTGATGCAGCAGATGCTGGATATGTAGTAGTCGATTTATTTGAAAGTTATCTCGACAAAGCCACAG
CATAG

Upstream 100 bases:

>100_bases
GCAAAAGCTGATTACCAAAGACGTCCTCATTGGCGTTGCACTGCCAAAATTACCTTAATTCTTTTGTAAAAATATATCGT
TACTTATATGGAGTAAAATT

Downstream 100 bases:

>100_bases
TTGATCAATACAAAATCACTGCCTTTAGGAGAATAAAAACATGACTGACGTTATATTGAATCCCACTAAATATGTTAACT
CCACACGCATTCCAGTGACT

Product: nitrile hydratase subunit beta

Products: NA

Alternate protein names: NHase; Nitrilase [H]

Number of amino acids: Translated: 214; Mature: 214

Protein sequence:

>214_residues
MDGFHDLGGVQGFGAIDHKINSKSYKPVFKKDWEHLAYTLMFLGAGHLNKFNIDQVRHAVERIGLRQHVGTEYYERYVIA
TATLLTEAGVITQQELDEALGSHFQLATPNHSTGRSAIADRPPFEVGDQVTVRDEYVSGHIRVPAYVRGKTGVVVHRTTE
KWPFPDAAGHGHTDALPQPTYHVEFNTQELWGDAADAGYVVVDLFESYLDKATA

Sequences:

>Translated_214_residues
MDGFHDLGGVQGFGAIDHKINSKSYKPVFKKDWEHLAYTLMFLGAGHLNKFNIDQVRHAVERIGLRQHVGTEYYERYVIA
TATLLTEAGVITQQELDEALGSHFQLATPNHSTGRSAIADRPPFEVGDQVTVRDEYVSGHIRVPAYVRGKTGVVVHRTTE
KWPFPDAAGHGHTDALPQPTYHVEFNTQELWGDAADAGYVVVDLFESYLDKATA
>Mature_214_residues
MDGFHDLGGVQGFGAIDHKINSKSYKPVFKKDWEHLAYTLMFLGAGHLNKFNIDQVRHAVERIGLRQHVGTEYYERYVIA
TATLLTEAGVITQQELDEALGSHFQLATPNHSTGRSAIADRPPFEVGDQVTVRDEYVSGHIRVPAYVRGKTGVVVHRTTE
KWPFPDAAGHGHTDALPQPTYHVEFNTQELWGDAADAGYVVVDLFESYLDKATA

Specific function: NHase catalyzes the hydration of various nitrile compounds to the corresponding amides [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrile hydratase subunit beta family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008990
- InterPro:   IPR003168 [H]

Pfam domain/function: NA

EC number: =4.2.1.84 [H]

Molecular weight: Translated: 23819; Mature: 23819

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDGFHDLGGVQGFGAIDHKINSKSYKPVFKKDWEHLAYTLMFLGAGHLNKFNIDQVRHAV
CCCCCCCCCCCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
ERIGLRQHVGTEYYERYVIATATLLTEAGVITQQELDEALGSHFQLATPNHSTGRSAIAD
HHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCEEEECCCCCCCCCCCCC
RPPFEVGDQVTVRDEYVSGHIRVPAYVRGKTGVVVHRTTEKWPFPDAAGHGHTDALPQPT
CCCCCCCCEEEEEHHHCCCEEECCEEEECCCCEEEEECCCCCCCCCCCCCCCCCCCCCCE
YHVEFNTQELWGDAADAGYVVVDLFESYLDKATA
EEEEECHHHHCCCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDGFHDLGGVQGFGAIDHKINSKSYKPVFKKDWEHLAYTLMFLGAGHLNKFNIDQVRHAV
CCCCCCCCCCCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
ERIGLRQHVGTEYYERYVIATATLLTEAGVITQQELDEALGSHFQLATPNHSTGRSAIAD
HHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCEEEECCCCCCCCCCCCC
RPPFEVGDQVTVRDEYVSGHIRVPAYVRGKTGVVVHRTTEKWPFPDAAGHGHTDALPQPT
CCCCCCCCEEEEEHHHCCCEEECCEEEECCCCEEEEECCCCCCCCCCCCCCCCCCCCCCE
YHVEFNTQELWGDAADAGYVVVDLFESYLDKATA
EEEEECHHHHCCCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2013568 [H]